Light‐Activated Decellularized Extracellular Matrix‐Based Bioinks for Volumetric Tissue Analogs at the Centimeter Scale

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 31(32)

Published: May 13, 2021

Abstract Tissue engineering requires not only tissue‐specific functionality but also a realistic scale. Decellularized extracellular matrix (dECM) is presently applied to the extrusion‐based 3D printing technology. It has demonstrated excellent efficiency as bioscaffolds that allow of living constructs with elaborate microarchitectures well biochemical milieu target tissues and organs. However, dECM bioinks have poor printability physical properties, resulting in limited shape fidelity scalability. In this study, new light‐activated ruthenium/sodium persulfate (dERS) are introduced. The materials can be polymerized via dityrosine‐based cross‐linking system rapid reaction kinetics improved mechanical properties. Complicated high aspect ratios fabricated similar geometry desired increased versatility using dERS. Furthermore, tissue safely regenerative capacity identical pure dECM. dERS may serve platform for wider biofabrication window through building complex centimeter‐scale supporting performances encapsulated cells. This capability opens avenues upscaling production hydrogel‐based without additional processes, applicable medicine.

Language: Английский

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Emerging silk fibroin materials and their applications: New functionality arising from innovations in silk crosslinking

Materials Today, Journal Year: 2023, Volume and Issue: 65, P. 244 - 259

Published: April 20, 2023

The ability to reverse engineer the silkworm fiber has led tremendous advancements in field of silk materials over last several decades, with fibroin applied biomedical, food, and high technology industries. This progress is largely due process into a regenerated solution that can be engineered variety material formats stabilized by reintroduction non-covalent native β-sheet protein structure. next revolution involves stabilizing through covalent crosslinking plasticization. These approaches have transformed not only strong, but also elastic flexible, making it compatible modern fabrication approaches. significantly broadened strategies include photolithography, digital light processing, extrusion-based 3D printing. As result, now used range applications including ocular prostheses, bio-adhesives, tissue engineering matrices, green biodegradable LEDs batteries, on-skin implantable sensors, bioplastics. In this review, we discuss evolution materials, focusing on tyrosine- methacrylate-based crosslinks, structural changes brough about plasticizing using glycerol calcium ions. We describe how advances development unique paving way for new across multiple industries, ushering era materials.

Language: Английский

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Peptide‐Based Coacervate‐Core Vesicles with Semipermeable Membranes

Advanced Materials, Journal Year: 2022, Volume and Issue: 34(34)

Published: July 7, 2022

Coacervates droplets have long been considered as potential protocells to mimic living cells. However, these lack a membrane and are prone coalescence, limiting their ability survive, interact, organize into higher-order assemblies. This work shows that tyrosine-rich peptide conjugates can undergo liquid-liquid phase separation in well-defined pH window transform stable membrane-enclosed by enzymatic oxidation cross-linking at the interface. The of peptides dityrosine creates semipermeable, flexible around coacervates with tunable thickness, which displays strong intrinsic fluorescence, stabilizes coacervate against coalescence. membranes an effective molecular weight cut-off 2.5 kDa, determined from partitioning small dyes labeled peptides, RNA, polymers protocells. Flicker spectroscopy reveals bending rigidity only 0.1kB T, is substantially lower than phospholipid bilayers despite larger thickness. Finally, it shown enzymes be stably encapsulated inside supplied substrates outside, opens way for membrane-bound compartments used molecularly crowded artificial cells capable communication or vehicle drug delivery.

Language: Английский

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Molecular Crowding: The History and Development of a Scientific Paradigm

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(6), P. 3186 - 3219

Published: March 11, 2024

It is now generally accepted that macromolecules do not act in isolation but "live" a crowded environment, is, an environment populated by numerous different molecules. The field of molecular crowding has its origins the far 80s became only end 90s. In present issue, we discuss various aspects are influenced and need to consider effects. This Review meant as introduction theme analysis evolution concept through time from colloidal polymer physics more biological perspective. We introduce themes will be thoroughly treated other Reviews issue. our intentions, each may stand itself, complete collection aspiration provide complementary perspectives propose holistic view crowding.

Language: Английский

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Self-propelled assembly of nanoparticles with self-catalytic regulation for tumour-specific imaging and therapy

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 11, 2024

Abstract Targeted assembly of nanoparticles in biological systems holds great promise for disease-specific imaging and therapy. However, the current manipulation nanoparticle dynamics is primarily limited to organic pericyclic reactions, which necessitate introduction synthetic functional groups as bioorthogonal handles on nanoparticles, leading complex laborious design processes. Here, we report synthesis tyrosine (Tyr)-modified peptides-capped iodine (I) doped CuS (CuS-I@P1 NPs) self-catalytic building blocks that undergo self-propelled inside tumour cells via Tyr-Tyr condensation reactions catalyzed by themselves. Upon cellular internalization, CuS-I@P1 NPs furin-guided formation CuS-I assemblies through dityrosine bond. The tumour-specific furin-instructed intracellular exhibits activatable dual-modal capability enhanced photothermal effect, enabling highly efficient therapy tumours. robust self-catalysis-regulated situ assembly, facilitated natural handles, offers advantages convenient fabrication, high reaction specificity, biocompatibility, representing a generalizable strategy target-specific biomedical

Language: Английский

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Tyrosine‐Rich Peptides as a Platform for Assembly and Material Synthesis

Advanced Science, Journal Year: 2018, Volume and Issue: 6(4)

Published: Nov. 15, 2018

Abstract The self‐assembly of biomolecules can provide a new approach for the design functional systems with diverse range hierarchical nanoarchitectures and atomically defined structures. In this regard, peptides, particularly short are attractive building blocks because their ease establishing structure–property relationships, productive synthesis, possibility hybridization other motifs. Several assembling such as ionic‐complementary cyclic peptide amphiphiles, Fmoc‐peptide, aromatic dipeptides, widely studied. Recently, studies on material synthesis application tyrosine‐rich peptide‐based have demonstrated that tyrosine units serve not only excellent assembly motifs but also multifunctional templates. Tyrosine has phenolic group contributes to π–π interactions conformation control efficient charge transport by proton‐coupled electron‐transfer reactions in natural systems. Here, critical roles motif respect its electrochemical, chemical, structural properties discussed recent discoveries advances made from self‐assembled structures peptide/inorganic hybrid materials highlighted. A brief account opportunities optimization applications biomimetic is included.

Language: Английский

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Growth‐Factor Free Multicomponent Nanocomposite Hydrogels That Stimulate Bone Formation

Advanced Functional Materials, Journal Year: 2020, Volume and Issue: 30(14)

Published: Feb. 16, 2020

Abstract Synthetic osteo‐promoting materials that are able to stimulate and accelerate bone formation without the addition of exogenous cells or growth factors represent a major opportunity for an aging world population. A co‐assembling system integrates hyaluronic acid tyramine ( HA‐Tyr ), bioactive peptide amphiphiles GHK‐Cu 2+ Laponite Lap ) engineer hydrogels with physical, mechanical, biomolecular signals can be tuned enhance regeneration is reported. The central design element multicomponent integration self‐assembly enzyme‐mediated oxidative coupling optimize structure mechanical properties in combination incorporation osteo‐ angio‐promoting segments facilitate signaling. Spectroscopic techniques used confirm interplay orthogonal covalent supramolecular interactions hydrogel formation. Furthermore, physico‐mechanical characterizations reveal exhibit improved compressive strength, stress relaxation profile, low swelling ratio, retarded enzymatic degradation compared single component hydrogels. Applicability validated vitro using human mesenchymal stem umbilical vein endothelial cells, vivo rabbit maxillary sinus floor reconstruction model. Animals treated HA‐Tyr‐HA‐Tyr‐GHK‐Cu significantly enhanced relative controls including commercially available Bio‐Oss.

Language: Английский

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Histidine as a key modulator of molecular self-assembly: Peptide-based supramolecular materials inspired by biological systems

Materials Today, Journal Year: 2022, Volume and Issue: 60, P. 106 - 127

Published: Aug. 28, 2022

Language: Английский

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A Strategy Based on the Enzyme-Catalyzed Polymerization Reaction of Asp-Phe-Tyr Tripeptide for Cancer Immunotherapy

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(13), P. 5127 - 5140

Published: March 25, 2021

Immunotherapy has provided a promising strategy for the treatment of cancers. However, even in tumors with high antigen burdens, systemic inhibition presentation still greatly restricts application immunotherapy. Here, we construct tumor protein-engineering system based on functional tripeptide, Asp-Phe-Tyr (DFY), which can automatically collect and deliver immunogenetic proteins from targeted cells to immune cells. Through tyrosinase-catalyzed polymerization, DFY tripeptide selectively accumulates tyrosinase high-expressed melanoma Then quinone-rich intermediates are covalently linked tumor-specific by Michael addition form protein-carried microfibers that could be engulfed antigen-presenting exhibited antigenic properties boosting effect. In deficient presentation, this successfully enrich transport antigen-containing Furthermore, vivo study murine melanoma, transdermal delivery suppressed growth postsurgery recurrence. Our findings provide an avenue regulation organism taking advantage certain polymerization reactions virtue chemical biology.

Language: Английский

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Strategies for improving the 3D printability of decellularized extracellular matrix bioink

Theranostics, Journal Year: 2023, Volume and Issue: 13(8), P. 2562 - 2587

Published: Jan. 1, 2023

3D bioprinting is a revolutionary technology capable of replicating native tissue and organ microenvironments by precisely placing cells into structures using bioinks.However, acquiring the ideal bioink to manufacture biomimetic constructs challenging.A natural extracellular matrix (ECM) an organ-specific material that provides physical, chemical, biological, mechanical cues are hard mimic small number components.Organ-derived decellularized ECM (dECM) has optimal properties.However, dECM always "non-printable" owing its poor properties.Recent studies have focused on strategies improve printability bioink.In this review, we highlight decellularization methods procedures used produce these bioinks, effective their printability, recent advances in regeneration dECM-based bioinks.Finally, discuss challenges associated with manufacturing bioinks potential large-scale applications.

Language: Английский

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