Targeting Bacteria‐Induced Ferroptosis of Bone Marrow Mesenchymal Stem Cells to Promote the Repair of Infected Bone Defects DOI Creative Commons
Kai Yuan, Yiqi Yang, Yixuan Lin

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(39)

Published: Aug. 21, 2024

Abstract The specific mechanisms underlying bacteria‐triggered cell death and osteogenic dysfunction in host bone marrow mesenchymal stem cells (BMSCs) remain unclear, posing a significant challenge to the repair of infected defects. This study identifies ferroptosis as predominant cause BMSCs microenvironment. Mechanistically, bacteria‐induced activation innate immune response leads upregulation phosphorylation interferon regulatory factor 7 (IRF7), thus facilitating IRF7‐dependent through transcriptional acyl‐coenzyme A synthetase long‐chain family member 4 (ACSL4). Moreover, it is found that intervening can partially rescue injuries dysfunction. Based on these findings, hydrogel composite 3D‐printed scaffold designed with reactive oxygen species (ROS)‐responsive release antibacterial quaternized chitosan sustained delivery inhibitor Ferrostatin‐1 (Fer‐1), capable eradicating pathogens promoting regeneration rat model Together, this suggests promising therapeutic target for defect repair.

Language: Английский

Combined photothermal and sonodynamic therapy using a 2D black phosphorus nanosheets loaded coating for efficient bacterial inhibition and bone-implant integration DOI
Junkai Zeng, Changjiang Gu, Xiangwu Geng

et al.

Biomaterials, Journal Year: 2023, Volume and Issue: 297, P. 122122 - 122122

Published: April 11, 2023

Language: Английский

Citations

91
Bioinspired Mild Photothermal Effect-Reinforced Multifunctional Fiber Scaffolds Promote Bone Regeneration DOI
Xiaodi Zhang, Qi Li, Longfei Li

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(7), P. 6466 - 6479

Published: March 30, 2023

Bone fractures are often companied with poor bone healing and high rates of infection. Early recruitment mesenchymal stem cells (MSCs) is critical for initiating efficient repair, mild thermal stimulation can accelerate the recovery chronic diseases. Here, a bioinspired, staged photothermal effect-reinforced multifunctional scaffold was fabricated repair. Uniaxially aligned electrospun polycaprolactone nanofibers were doped black phosphorus nanosheets (BP NSs) to endow excellent near-infrared (NIR) responsive capability. Apt19S then decorated on surface selectively recruit MSCs toward injured site. Afterward, microparticles phase change materials loaded antibacterial drugs also deposited scaffold, which could undergo solid-to-liquid transition above 39 °C, triggering release payload eliminate bacteria prevent Under NIR irradiation, photothermal-mediated up-regulation heat shock proteins accelerated biodegradation BP NSs promote osteogenic differentiation biomineralization. Overall, this strategy shows ability elimination, recruitment, regeneration promotion assistance effect in vitro vivo, emphasizes design bioinspired its potential tissue engineering.

Language: Английский

Citations

86
GelMA-based bioactive hydrogel scaffolds with multiple bone defect repair functions: therapeutic strategies and recent advances DOI Creative Commons

Bixia Zhou,

Xulei Jiang, Xinxin Zhou

et al.

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Feb. 9, 2023

Currently, the clinical treatment of critical bone defects attributed to various causes remains a great challenge, and repairing these with synthetic substitutes is most common strategy. In general, tissue engineering materials that mimic structural, mechanical biological properties natural have been extensively applied fill promote in situ regeneration. Hydrogels extracellular matrix (ECM)-like are materials, among which methacrylate-based gelatin (GelMA) hydrogels widely used because their tunable properties, excellent photocrosslinking capability good biocompatibility. Owing lack osteogenic activity, however, GelMA combined other types activities improve current composites. There three main aspects consider when enhancing regenerative performance composite materials: osteoconductivity, vascularization osteoinduction. Bioceramics, bioglass, biomimetic scaffolds, inorganic ions, bionic periosteum, growth factors two-dimensional (2D) nanomaterials combinations achieve enhanced regeneration activities. Three-dimensional (3D)-bioprinted scaffolds popular research topic (BTE), printed customized suitable for restoring large irregular due shape structural tunability, Herein, recent progress on GelMA-based hydrogel as multifunctional platforms plastic or orthopedic clinics systematically reviewed summarized. These strategies pave way design effective reconstruction biosafety. This review provides novel insights into development trends (BTE) correcting defects, contents summarized emphasized from perspectives (osteoconductivity, vascularization, osteoinduction 3D-bioprinting). addition, advantages deficiencies put forward, corresponding improvement measures presented prior application near future (created BioRender.com).

Language: Английский

Citations

83
Recent advances in GelMA hydrogel transplantation for musculoskeletal disorders and related disease treatment DOI Creative Commons
Bin Lv, Lu Li,

Liangcong Hu

et al.

Theranostics, Journal Year: 2023, Volume and Issue: 13(6), P. 2015 - 2039

Published: Jan. 1, 2023

Increasing data reveals that gelatin has been methacrylated is involved in a variety of physiologic processes are important for therapeutic interventions. Gelatin methacryloyl (GelMA) hydrogel highly attractive hydrogels-based bioink because its good biocompatibility, low cost, and photo-cross-linking structure useful cell survivability monitoring. Methacrylated established itself as typical composition with extensive biomedical applications. Recent advances GelMA have focused on integrating them bioactive functional nanomaterials, the goal improving GelMA's physical, chemical, biological properties. ability to modify characteristics due synthesis technique also makes it choice soft hard tissues. become an independent or supplementary technology musculoskeletal problems. Here, we systematically review mechanism-of-action, uses, challenges future direction disorders. We give overview nanocomposite different applications disorders, such osteoarthritis, intervertebral disc degeneration, bone regeneration, tendon disorders so on.

Language: Английский

Citations

80
Nanomaterials in Antibacterial Photodynamic Therapy and Antibacterial Sonodynamic Therapy DOI

Chaonan He,

Peipei Feng, Mingming Hao

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(38)

Published: April 27, 2024

Abstract Antibacterial photodynamic therapy (aPDT) and antibacterial sonodynamic (aSDT) utilize sensitizers (photosensitizers/sonosensitizers) to produce reactive oxygen species (ROS) for treatment under the stimulation of light/ultrasound, which have characteristics broad‐spectrum properties, low drug‐resistance, effective targeting infected tissues. Nanomaterials in aPDT/aSDT are primarily used as nano‐sensitizers or nano‐carriers sensitizers. They enhance stability permeability sensitizers, improve strengthen photodynamic/sonodynamic properties (modification absorption efficiency light/ultrasonic response stress by modulation nanoparticle shape, size, structure). Also, they modifiability (controlling release rate time sensitizer needed optimize therapeutic effect), programmability multifunctionality (flexible application nanotechnology designing with multiple functions, such drug delivery, targeted therapy, monitoring), expand possibilities combination therapies (the can be loaded other agents, enabling therapies). expected further promote development achieve improved effects. This review summarizes progress nanomaterials recent years based on current strategies provide a theoretical reference aPDT/aSDT.

Language: Английский

Citations

54
Zinc‐Based Tannin‐Modified Composite Microparticulate Scaffolds with Balanced Antimicrobial Activity and Osteogenesis for Infected Bone Defect Repair DOI
Yitao Zhao, Jintao Li, Lingli Liu

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(20)

Published: March 25, 2023

Treatment of infected bone defects is a major clinical challenge; bioactive materials combining sufficient antimicrobial activity and favorable osteogenic ability are urgently needed. In this study, through facile one-pot hydrothermal reaction zinc acetate in the presence tannic acid (TA), with or without silver nitrate (AgNO3 ), used to synthesize TA nanoparticles (Ag NPs) bulk-modified oxide (ZnO) (ZnO-TA ZnO-TA-Ag), which further composited zein fabricate porous microparticulate scaffolds for defect repair. Bulk modification significantly improves release rate antibacterial metal ions (Zn2+ >100 times that ZnO). Fast long-lasting (>35 d) Zn2+ Ag+ guaranteed capability excellent properties promoting differentiation marrow mesenchymal stem cells endogenous citric production mineralization providing considerable immunomodulatory M2 polarization macrophages. At same time, synchronously-released could scavenge reactive oxygen species (ROS) ROS produced by ions, effectively balancing osteogenesis sufficiently control infection while protecting surrounding tissue from damage, thus

Language: Английский

Citations

46
Multifunctional Conductive and Electrogenic Hydrogel Repaired Spinal Cord Injury via Immunoregulation and Enhancement of Neuronal Differentiation DOI
Mingshan Liu, Wencan Zhang,

Shuwei Han

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(21)

Published: Feb. 3, 2024

Abstract Spinal cord injury (SCI) is a refractory neurological disorder. Due to the complex pathological processes, especially secondary inflammatory cascade and lack of intrinsic regenerative capacity, it difficult recover function after SCI. Meanwhile, simulating conductive microenvironment spinal reconstructs electrical neural signal transmission interrupted by SCI facilitates repair. Therefore, double‐crosslinked hydrogel (BP@Hydrogel) containing black phosphorus nanoplates (BP) synthesized. When placed in rotating magnetic field (RMF), BP@Hydrogel can generate stable signals exhibit electrogenic characteristic. In vitro, shows satisfactory biocompatibility alleviate activation microglia. RMF, enhances anti‐inflammatory effects. wireless stimulation promotes differentiation stem cells (NSCs) into neurons, which associated with PI3K/AKT pathway. vivo, injectable elicit behavioral electrophysiological recovery complete transected mice alleviating inflammation facilitating endogenous NSCs form functional neurons synapses under RMF. The present research develops multifunctional for repair targeting multiple mechanisms including immunoregulation enhancement neuronal differentiation.

Language: Английский

Citations

37
Electroactive Biomaterials Regulate the Electrophysiological Microenvironment to Promote Bone and Cartilage Tissue Regeneration DOI
Li Chen,

Jianye Yang,

Zhengwei Cai

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(23)

Published: Jan. 7, 2024

Abstract The incidence of large bone and articular cartilage defects caused by traumatic injury is increasing worldwide; the tissue regeneration process for these injuries lengthy due to limited self‐healing ability. Endogenous bioelectrical phenomenon has been well recognized play an important role in homeostasis regeneration. Studies have reported that electrical stimulation (ES) can effectively regulate various biological processes holds promise as external intervention enhance synthesis extracellular matrix, thereby accelerating Hence, electroactive biomaterials considered a biomimetic approach ensure functional recovery integrating physiological signals, including electrical, biochemical, mechanical signals. This review will discuss endogenous bioelectricity tissue, effects ES on cellular behaviors. Then, recent advances materials their applications are systematically overviewed, with focus advantages disadvantages repair performances modulation cell fate. Finally, significance mimicking electrophysiological microenvironment target emphasized future development challenges strategies proposed.

Language: Английский

Citations

24
Effects of Electric Field‐Modulated Conductive Hydrogel on Osseoperception and Osseointegration of Dental Implants DOI
Wen Qin, Ling Li, Wen Niu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(28)

Published: March 14, 2024

Abstract Achieving optimal implant restoration hinges on both robust osseointegration as the structural foundation and favorable osseoperception for advanced masticatory function. The significance of nerve innervation in peri‐implant environment cornerstone is often underestimated. Despite integral role endogenous electric fields (EFs) human body, particularly electrosensitive tissues like tissue, current approach involving external electrical stimulation invasive not clinically applicable. present study introduces a conductive hydrogel designed to respond EFs, aiming foster regeneration around dental implants coordinate osseoperception. promotes neurite outgrowth by upregulating intracellular Ca 2+ concentration activating subsequent pathways. Furthermore, enhanced release neuropeptides from neurocells improves osteogenesis osteoblasts. impact also thoroughly investigated vivo. This represents unique strategy enhancing within EFs environment. advancement opens door achieving physiological psychological integration implants.

Language: Английский

Citations

22
Recent Advances in Two-Dimensional Nanomaterials for Healthcare Monitoring DOI

Arun Kumar Manoharan,

Mohamed Ismail Kamal Batcha,

Shanmugam Mahalingam

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 1706 - 1734

Published: April 2, 2024

The development of advanced technologies for the fabrication functional nanomaterials, nanostructures, and devices has facilitated biosensors analyses. Two-dimensional (2D) with unique hierarchical structures, a high surface area, ability to be functionalized target detection at surface, exhibit potential biosensing applications. electronic properties, mechanical flexibility, optical, electrochemical, physical properties 2D nanomaterials can easily modulated, enabling construction platforms various analytes targeted recognition, sensitivity, selectivity. This review provides an overview recent advances in nanostructures used biosensor wearable-sensor healthcare health-monitoring Finally, advantages 2D-nanomaterial-based several challenges their optimal operation have been discussed facilitate smart high-performance future.

Language: Английский

Citations

19