The role of the immune microenvironment in bone, cartilage, and soft tissue regeneration: from mechanism to therapeutic opportunity

Military Medical Research, Journal Year: 2022, Volume and Issue: 9(1)

Published: Nov. 19, 2022

Abstract Bone, cartilage, and soft tissue regeneration is a complex spatiotemporal process recruiting variety of cell types, whose activity interplay must be precisely mediated for effective healing post-injury. Although extensive strides have been made in the understanding immune microenvironment processes governing bone, regeneration, clinical translation these mechanisms remains challenge. Regulation increasingly becoming favorable target regeneration; therefore, an in-depth communication between cells functional would valuable. Herein, we review regulatory role promotion maintenance stem states context repair regeneration. We discuss roles various subsets introduce novel strategies, example, biomaterial-targeting activity, aimed at regulating healing. Understanding crosstalk pathways may shed light on new therapeutic opportunities enhancing through regulation microenvironment.

Language: Английский

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Smart‐Responsive Multifunctional Therapeutic System for Improved Regenerative Microenvironment and Accelerated Bone Regeneration via Mild Photothermal Therapy

Advanced Science, Journal Year: 2023, Volume and Issue: 11(2)

Published: Nov. 7, 2023

Abstract The treatment of bone defects remains a substantial clinical challenge due to the lack spatiotemporal management immune microenvironment, revascularization, and osteogenic differentiation. Herein, deferoxamine (DFO)‐loaded black phosphorus nanosheets decorated by polydopamine layer are prepared (BPPD) compounded into gelatin methacrylate/sodium alginate methacrylate (GA) hybrid hydrogel as smart‐responsive therapeutic system (GA/BPPD) for accelerated regeneration. BPPD nanocomposites served bioactive components near‐infrared (NIR) photothermal agents, which conferred with excellent NIR/pH dual‐responsive properties, realizing stimuli‐responsive release DFO PO 4 3 − during Under action NIR‐triggered mild therapy, GA/BPPD exhibited positive effect on promoting osteogenesis angiogenesis, eliminating excessive reactive oxygen species, inducing macrophage polarization M2 phenotype. More significantly, through polarization‐induced osteoimmune this platform could also drive functional cytokine secretion enhanced angiogenesis osteogenesis. In vivo experiments further demonstrated that facilitate healing attenuating local inflammatory response, increasing pro‐healing factors, stimulating endogenous cell recruitment, accelerating revascularization. Collectively, proposed intelligent provides promising strategy reshape damaged tissue microenvironment augmented

Language: Английский

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Supramolecular Peptide Nanofiber Hydrogels for Bone Tissue Engineering: From Multihierarchical Fabrications to Comprehensive Applications

Advanced Science, Journal Year: 2022, Volume and Issue: 9(11)

Published: Feb. 7, 2022

Abstract Bone tissue engineering is becoming an ideal strategy to replace autologous bone grafts for surgical repair, but the multihierarchical complexity of natural still difficult emulate due lack suitable biomaterials. Supramolecular peptide nanofiber hydrogels (SPNHs) are emerging biomaterials because their inherent biocompatibility, satisfied biodegradability, high purity, facile functionalization, and tunable mechanical properties. This review initially focuses on fabrications by SPNHs bony extracellular matrix. Structurally, supramolecular peptides based distinctive building blocks can assemble into hydrogels, which be used as nanomorphology‐mimetic scaffolds engineering. Biochemically, bioactive motifs factors covalently tethered or physically absorbed endow various functions depending physiological pharmacological requirements. Mechanically, four strategies summarized optimize biophysical microenvironment regeneration. Furthermore, comprehensive applications about reviewed. The directly in form injectable composite nanoscaffolds, they construct engineered bioprinting bioreactors. Finally, continuing challenges outlook discussed.

Language: Английский

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Recent advances of electrospun nanofiber-enhanced hydrogel composite scaffolds in tissue engineering

Journal of Manufacturing Processes, Journal Year: 2024, Volume and Issue: 123, P. 112 - 127

Published: June 5, 2024

Language: Английский

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Bioinspired soft-hard combined system with mild photothermal therapeutic activity promotes diabetic bone defect healing via synergetic effects of immune activation and angiogenesis

Theranostics, Journal Year: 2024, Volume and Issue: 14(10), P. 4014 - 4057

Published: Jan. 1, 2024

Background:The comprehensive management of diabetic bone defects remains a substantial clinical challenge due to the hostile regenerative microenvironment characterized by aggravated inflammation, excessive reactive oxygen species (ROS), bacterial infection, impaired angiogenesis, and unbalanced homeostasis.Thus, an advanced multifunctional therapeutic platform capable simultaneously achieving immune regulation, elimination, tissue regeneration is urgently designed for augmented under pathological milieu.Methods Results: Herein, photoactivated soft-hard combined scaffold system (PGCZ) was engineered introducing polydopamine-modified zeolitic imidazolate framework-8-loaded double-network hydrogel (soft matrix component) into 3D-printed poly(ε-caprolactone) (PCL) (hard component).The versatile PGCZ based on PCL thus prepared features highly extracellular matrix-mimicking microstructure, suitable biodegradability mechanical properties, excellent photothermal performance, allowing long-term structural stability support regeneration.Under periodic near-infrared (NIR) irradiation, localized effect triggers on-demand release Zn 2+ , which, together with repeated mild hyperthermia, collectively accelerates proliferation osteogenic differentiation preosteoblasts potently inhibits growth biofilm formation.Additionally, also presents outstanding immunomodulatory ROS scavenging capacities, which regulate M2 polarization macrophages drive functional cytokine secretion, leading pro-regenerative in situ enhanced vascularization.In vivo experiments further demonstrated that conjunction activity remarkably attenuated local inflammatory cascade, initiated endogenous stem cell recruitment neovascularization, orchestrated osteoblast/osteoclast balance, ultimately accelerating regeneration. Ivyspring

Language: Английский

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Current Perspectives of Protein in Bone Tissue Engineering: Bone Structure, Ideal Scaffolds, Fabrication Techniques, Applications, Scopes, and Future Advances

ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(8), P. 5082 - 5106

Published: July 15, 2024

In view of their exceptional approach, excellent inherent biocompatibility and biodegradability properties, interaction with the local extracellular matrix, protein-based polymers have received attention in bone tissue engineering, which is a multidisciplinary field that repairs regenerates fractured bones. Bone multihierarchical complex structure, it performs several essential biofunctions, including maintaining mineral balance structural support protecting soft organs. Protein-based gained interest developing ideal scaffolds as emerging biomaterials for healing regeneration, challenging to design substitutes perfect biomaterials. Several polymers, collagen, keratin, gelatin, serum albumin, etc., are potential materials due cytocompatibility, controlled biodegradability, high biofunctionalization, tunable mechanical characteristics. While numerous studies indicated encouraging possibilities proteins BTE, there still major challenges concerning stability physiological conditions, continuous release growth factors bioactive molecules. Robust derived from can be used replace broken or diseased biocompatible substitute; proteins, being biopolymers, provide engineering. Herein, recent developments protein cutting-edge engineering addressed this review within 3–5 years, focus on significant future perspectives. The first section discusses fundamentals anatomy scaffolds, second describes fabrication techniques scaffolds. third highlights importance applications BTE. Hence, development state-of-the-art has been discussed, highlighting

Language: Английский

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Copper Ion‐Modified Germanium Phosphorus Nanosheets Integrated with an Electroactive and Biodegradable Hydrogel for Neuro‐Vascularized Bone Regeneration

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(27)

Published: July 8, 2023

Severe bone defects accompanied by vascular and peripheral nerve injuries represent a huge orthopedic challenge are often the risk of infection. Thus, biomaterials with antibacterial neurovascular regeneration properties highly desirable. Here, newly designed biohybrid biodegradable hydrogel (GelMA) containing copper ion-modified germanium-phosphorus (GeP) nanosheets, which act as neuro-vascular agents, is designed. The ion modification process serves to improve stability GeP nanosheets offers platform for sustained release bioactive ions. Study findings show that GelMA/GeP@Cu has effective properties. integrated can significantly boost osteogenic differentiation marrow mesenchymal stem cells, facilitate angiogenesis in human umbilical vein endothelial up-regulate neural differentiation-related proteins cells vitro. In vivo, rat calvarial defect mode, found enhance neurogenesis, eventually contributing regeneration. These indicate field tissue engineering, serve valuable biomaterial neuro-vascularized infection prevention.

Language: Английский

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A comprehensive review on peptide-bearing biomaterials: From ex situ to in situ self-assembly

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 502, P. 215600 - 215600

Published: Dec. 14, 2023

Language: Английский

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Short Peptide Nanofiber Biomaterials Ameliorate Local Hemostatic Capacity of Surgical Materials and Intraoperative Hemostatic Applications in Clinics

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(39)

Published: March 21, 2023

Short designer self-assembling peptide (dSAP) biomaterials are a new addition to the hemostat group. It may provide diverse and robust toolbox for surgeons integrate wound microenvironment with much safer stronger hemostatic capacity than conventional materials agents. Especially in noncompressible torso hemorrhage (NCTH), diffuse mucosal surface bleeding, internal medical bleeding (IMB), respect optimal formulation, dSAP ingenious nanofiber alternatives make bioactive neural scaffold, nasal packing, large coverage gastrointestinal surgery (esophagus, gastric lesion, duodenum, lower digestive tract), epicardiac cell-delivery carrier, transparent matrix barrier, so on. Herein, multiple surgical specialties, dSAP-biomaterial-based nano-hemostats achieve safe, effective, immediate hemostasis, facile healing, potentially reduce risks delayed rebleeding, post-operative or related complications. The biosafety vivo, indications, tissue-sealing quality, feasibility, local usability addressed comprehensively sequentially pursued develop useful techniques better performance. Here, state of art all-round advancements nano-hemostatic approaches provided. Relevant critical insights will inspire exciting investigations on nanotechnology, next-generation biomaterials, promising prospects clinics.

Language: Английский

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Designed peptide amphiphiles as scaffolds for tissue engineering

Advances in Colloid and Interface Science, Journal Year: 2023, Volume and Issue: 314, P. 102866 - 102866

Published: Feb. 27, 2023

Language: Английский

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