A Recombinant Parathyroid Hormone‐Related Peptide Locally Applied in Osteoporotic Bone Defect DOI Creative Commons
Yi Wang, Yingkun Hu, Shenghui Lan

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(22)

Published: May 25, 2023

Abstract The local application of drug‐loaded bioactive scaffold materials is one the important directions to solve clinical problem osteoporotic (OP) bone defects. This study retains advantages drug loading and mechanical properties natural 3D scaffolds. scaffolds are functionally modified through chemical self‐assembly approaches with polydopamine (PDA) nanoparticles parathyroid hormone‐related peptide‐1 (PTHrP‐1) for efficient loading. investigates effects novel on ossification, osteoclastogenesis, macrophage polarization. work elucidates in regulating osteoclastic activity new formation vitro. Further studies establishment repair OP defects small animals conducted, potential porous promote initially verified. preparation safe economical anti‐OP material provides a theoretical basis translational applications.

Language: Английский

A Comprehensive Review on Silk Fibroin as a Persuasive Biomaterial for Bone Tissue Engineering DOI Open Access
Minghui Li, Jiaqian You,

Qiuyue Qin

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(3), P. 2660 - 2660

Published: Jan. 31, 2023

Bone tissue engineering (BTE) utilizes a special mix of scaffolds, cells, and bioactive factors to regulate the microenvironment bone regeneration form three-dimensional simulation structure regenerate tissue. Silk fibroin (SF) is perhaps most encouraging material for BTE given its tunable mechanical properties, controllable biodegradability, excellent biocompatibility. Numerous studies have confirmed significance SF stimulating formation. In this review, we start by introducing characteristics SF. After that, immunological mechanism osteogenesis summarized, various forms biomaterials latest development prospects in are emphatically introduced. Biomaterials based on great potential engineering, review will serve as resource future design research.

Language: Английский

Citations

28
Sericin/Nano-Hydroxyapatite Hydrogels Based on Graphene Oxide for Effective Bone Regeneration via Immunomodulation and Osteoinduction DOI Creative Commons

Mei Fu,

Jun Li, Mingchong Liu

et al.

International Journal of Nanomedicine, Journal Year: 2023, Volume and Issue: Volume 18, P. 1875 - 1895

Published: April 1, 2023

Immune responses and osteogenesis differentiation induced by implants are crucial for bone tissue regeneration. Consideration of only one those properties is not sufficient. To investigate the synergistic actions, we designed alginate/graphene oxide/sericin/nanohydroxyapatite (Alg/GO/Ser/nHAP) nanocomposite hydrogels with both osteoimmunomodulatory osteoinductive activities. This study aimed to explore effect hydrogel on promoting marrow stem cells (BMSCs).Alg/GO/Ser/nHAP was fabricated characterized SEM, FTIR, XRD, stress-strain, rheology, swelling degradation. After impact sericin M2 macrophage polarization identified, BMSCs viability adhesion were evaluated CCK8 assay, live/dead staining, cytoskeleton staining. The cell osteogenic observed ALP/ARS immunofluorescence RT-PCR, Western blotting, respectively. Rat cranial defect model used assess effects scaffolds in vivo micro‑computed tomographic, histological, immunohistochemical analyses after 8 weeks healing.In vitro experiments revealed that presented desirable mechanical strength, stability, porosity, biocompatibility. Significantly, nHAP appeared exert Sericin inhibit immune response inducing M2-type create a positive osteoimmune microenvironment, contributing improving osseointegration at bone-implant interface promote osteogenesis. However, rat further enhanced combining hydrogel. Eventually, implanted into model, assisting reduction local inflammation efficient regeneration.The stimulated formation immunomodulation direct induction nHAP, demonstrating such scaffold modulates microenvironment promising approach development engineering future.

Language: Английский

Citations

26
Advanced injectable hydrogels for bone tissue regeneration DOI

Hassan Thoulfikar A.Alamir,

Ghufran Lutfi Ismaeel, Abduladheem Turki Jalil

et al.

Biophysical Reviews, Journal Year: 2023, Volume and Issue: 15(2), P. 223 - 237

Published: April 1, 2023

Language: Английский

Citations

26
Application of functional peptides in the electrochemical and optical biosensing of cancer biomarkers DOI
Yue Cao, Liang Zhou,

Zhikai Fang

et al.

Chemical Communications, Journal Year: 2023, Volume and Issue: 59(23), P. 3383 - 3398

Published: Jan. 1, 2023

This review aims to provide an overview of recent advances in the field functional peptide-based biosensing cancer biomarkers. Particular attention is focused on use electrochemical and optical techniques.

Language: Английский

Citations

25
Self-Assembling Peptide Nanofibers Anchored Parathyroid Hormone Derivative for Bone Tissue Engineering DOI
Zhuowen Hao, Tianhong Chen, Ying Wang

et al.

Advanced Fiber Materials, Journal Year: 2024, Volume and Issue: 6(2), P. 583 - 606

Published: Feb. 21, 2024

Language: Английский

Citations

14
Osteoporotic osseointegration: therapeutic hallmarks and engineering strategies DOI Creative Commons
Xiaocui Chen, Zhuowen Hao, Hanke Li

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(10), P. 3859 - 3899

Published: Jan. 1, 2024

Osteoporosis is a systemic skeletal disease caused by an imbalance between bone resorption and formation. Current treatments primarily involve medication hormone therapy. However, these lack directionality are often ineffective for locally severe osteoporosis, with the potential complex adverse reactions. Consequently, treatment strategies using bioactive materials or external interventions have emerged as most promising approaches. This review proposes twelve microenvironmental targets osteoporosis-related pathological changes, including local accumulation of inflammatory factors reactive oxygen species (ROS), mitochondrial dynamics, insulin resistance, disruption cell autophagy, apoptosis, changes in neural secretions, aging cells, increased tissue vascular destruction, decreased regeneration. Additionally, this examines current research status effective biophysical biochemical stimuli based on summarizes advantages optimal parameters different bioengineering to support preclinical clinical osteoporosis Finally, addresses ongoing challenges future prospects.

Language: Английский

Citations

14
Enhancing calvarial defects repair with PDGF-BB mimetic peptide hydrogels DOI
Xia Wu, Mingming Yan,

Jun Shen

et al.

Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 370, P. 277 - 286

Published: May 1, 2024

Language: Английский

Citations

12
Therapeutic Potential of Nanocarrier Mediated Delivery of Peptides for Wound Healing: Current Status, Challenges and Future Prospective DOI
Mohit Kumar,

Dikshant Kumar,

Devesh Kumar

et al.

AAPS PharmSciTech, Journal Year: 2024, Volume and Issue: 25(5)

Published: May 10, 2024

Language: Английский

Citations

11
Polysaccharide-Based Hydrogels for Advanced Biomedical Engineering Applications DOI Creative Commons
Md. Mahamudul Hasan Rumon, Anwarul Azim Akib, Stephen Don Sarkar

et al.

ACS Polymers Au, Journal Year: 2024, Volume and Issue: 4(6), P. 463 - 486

Published: Aug. 20, 2024

In recent years, numerous applications of hydrogels using polysaccharides have evolved, benefiting from their widespread availability, excellent biodegradability, biocompatibility, and nonpoisonous nature. These natural polymers are typically sourced renewable materials or manufacturing processes, contributing collaboratively to waste management demonstrating the potential for enhanced enduring sustainability. field novel bioactive molecule carriers biotherapeutics, attracting attention due inherent properties adaptable chemical structures. offer versatile matrices with a range architectures mechanical properties, while retaining bioactivity incorporated biomolecules. However, conventional polysaccharide-based suffer inadequate toughness large swelling which prohibit efficacy in real-world applications. This review offers insights into latest advancements development diverse biotherapeutic administrations, either standalone conjunction other drug delivery systems, pharmaceutical biomedical fields.

Language: Английский

Citations

11
Investigating the Role of Amino Acids in Short Peptides for Hydroxyapatite Binding and Osteogenic Differentiation of Mesenchymal Stem Cells to Aid Bone Regeneration DOI

Moumita Halder,

Ananya Singh,

Deepa Negi

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(4), P. 2286 - 2301

Published: March 19, 2024

Bone defects show a slow rate of osteoconduction and imperfect reconstruction, the current treatment strategies to treat bone suffer from limitations like immunogenicity, lack cell adhesion, absence osteogenic activity. In this context, bioactive supramolecular peptides peptide gels offer unique opportunities develop biomaterials that can play dominant role in biomineralization tissues promote formation. article, we have demonstrated potential six tetrapeptides for specific binding hydroxyapatite (HAp), major inorganic component bone, their effect on growth differentiation mesenchymal stem cells (MSCs). We adopted simplistic approach rationally designing amphiphilic by incorporating amino acids, Ser, pSer, Pro, Hyp, Asp, Glu, which are present either collagenous or noncollagenous proteins render properties antioxidant, calcification, mineralization. A total tetrapeptides, Trp–Trp–His–Ser (WWHS), Trp–Trp–His–pSer (WWHJ), Trp–Trp–His–Pro (WWHP), Trp–Trp–His–Hyp (WWHO), Trp–Trp–His–Asp (WWHD), Trp–Trp–His–Glu (WWHE), were synthesized. Four found self-assemble into nanofibrillar resembling extracellular matrix (ECM), remaining two (WWHJ, WWHP) self-assembled nanorods. The showed excellent encapsulation, proliferation, migration induced (MSCs), as evident enhanced mineralization, resulting upregulation markers, RUNX 2, COL I, OPN, OCN, alkaline phosphatase (ALP) production, calcium deposition. also downregulation inflammatory TNF-α iNOS, anti-inflammatory marker, IL-10, M2 macrophage polarization. RANKL TRAP genes downregulated coculture system MC3T3-E1 RAW 264.7 cells, implying osteogenesis inhibit osteoclastogenesis. peptide-based developed work enhance regeneration capacity strong scaffolds tissue engineering.

Language: Английский

Citations

9