Dual-sided centripetal microgrooved poly (D,L-lactide-co-caprolactone) disk encased in immune-regulating hydrogels for enhanced bone regeneration

Materials Today Bio, Journal Year: 2025, Volume and Issue: 30, P. 101436 - 101436

Published: Jan. 5, 2025

Well-designed artificial scaffolds are urgently needed due to the limited self-repair capacity of bone, which hampers effective regeneration in critical defects. Optimal must provide physical guidance recruit cells and immune regulation improve regenerative microenvironment. This study presents a novel scaffold composed dual-sided centripetal microgrooved poly(D,L-lactide-co-caprolactone) (PLCL) film combined with dynamic hydrogel containing prednisolone (PLS)-loaded Prussian blue nanoparticles (PB@PLS). The microgrooves on surface PLCL were imprinted using micropatterned polydimethylsiloxane (PDMS) template. Following aminolysis, was covalently grafted EM-7 peptide via glutaraldehyde. Functional group analysis, morphology hydrophilicity evaluated X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), an optical contact angle measuring instrument, respectively. Bone regeneration-related (e.g., bone marrow mesenchymal stem cells, macrophages, Schwann endothelial cells) cultured films tended align along stripes migrate from periphery toward center region vitro. Subsequently, encapsulated immune-regulating synthesized thiol-modified gelatin Cu2+ presence PB@PLS nanoparticles, demonstrated excellent antioxidant properties. significantly accelerated critical-sized regeneration, as evidenced by increase volume newly formed histological images vivo. innovative approach holds substantial promise for clinical applications broader tissue repair.

Language: Английский

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Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

Advanced Science, Journal Year: 2023, Volume and Issue: 10(18)

Published: April 25, 2023

The rapid degradation of magnesium (Mg) alloy implants erodes mechanical performance and interfacial bioactivity, thereby limiting their clinical utility. Surface modification is among the solutions to improve corrosion resistance bioefficacy Mg alloys. Novel composite coatings that incorporate nanostructures create new opportunities for expanded use. Particle size dominance impermeability may increase prolong implant service time. Nanoparticles with specific biological effects be released into peri-implant microenvironment during promote healing. Composite nanocoatings provide nanoscale surfaces cell adhesion proliferation. activate cellular signaling pathways, while those porous or core-shell structures carry antibacterial immunomodulatory drugs. vascular reendothelialization osteogenesis, attenuate inflammation, inhibit bacterial growth, thus increasing applicability in complex microenvironments such as atherosclerosis open fractures. This review combines physicochemical properties efficiency Mg-based biomedical summarize advantages nanocoatings, analyzes mechanisms action, proposes design construction strategies, purpose providing a reference promoting application further nanocoatings.

Language: Английский

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Magnetic Nanoparticles and Methylprednisolone Based Physico‐Chemical Bifunctional Neural Stem Cells Delivery System for Spinal Cord Injury Repair

Advanced Science, Journal Year: 2024, Volume and Issue: 11(21)

Published: March 22, 2024

Abstract Neural stem cells (NSCs) transplantation is an attractive and promising treatment strategy for spinal cord injury (SCI). Various pathological processes including the severe inflammatory cascade difficulty in stable proliferation differentiation of NSCs limit its application translation. Here, a novel physico‐chemical bifunctional neural delivery system containing magnetic nanoparticles (MNPs methylprednisolone (MP) designed to repair SCI, former regulates through mechanical stimulation chronic phase, while latter alleviates response acute phase. The releases MP promote microglial M2 polarization, inhibit M1 reduce neuronal apoptosis. Meanwhile, tend differentiate into functional neurons with generated by MNPs static field, which related activation PI3K/AKT/mTOR pathway. SCI mice achieve better recovery after receiving via system, has milder inflammation, higher number microglia, more neurons, axonal regeneration. Together, this combined physical chemical drug therapy demonstrated be effective, provides new insights clinical transformation repair.

Language: Английский

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Electrospun Composite PLLA‐PPSB Nanofiber Nerve Conduits for Peripheral Nerve Defects Repair and Regeneration

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(10)

Published: Jan. 18, 2024

Peripheral nerve injury (PNI) is a common clinical problem and regenerating peripheral defects remain significant challenge. Poly(polyol sebacate) (PPS) polymers are developed as promising materials for biomedical applications due to their biodegradability, biocompatibility, elastomeric properties, ease of production. However, the application PPS-based biomaterials in tissue engineering, especially PNI repair, limited. In this study, composite nanofibers poly(l-lactic acid)-poly(polycaprolactone triol-co-sebacic acid-co-N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt) (PLLA-PPSB) aimed construct through electrospinning assess vitro biocompatibility with Schwann cells (SCs) vivo repair capabilities defects. For first time, bioactivity nanomaterial examined at molecular, cellular, animal levels repair. Electrospun PLLA-PPSB display favorable physicochemical properties providing an effective interface proliferation, glial expression, adhesion SCs vitro. experiments using 10-mm rat sciatic defect model show that nanofiber conduits enhance myelin formation, axonal regeneration, angiogenesis, functional recovery. Transcriptome analysis biological validation indicate may promote SC proliferation by activating PI3K/Akt signaling pathway. This suggests potential

Language: Английский

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Nanoparticle-Facilitated Therapy: Advancing Tools in Peripheral Nerve Regeneration

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 19 - 34

Published: Jan. 1, 2024

Abstract: Peripheral nerve injuries, arising from a diverse range of etiologies such as trauma and underlying medical conditions, pose substantial challenges in both clinical management subsequent restoration functional capacity. Addressing these challenges, nanoparticles have emerged promising therapeutic modality poised to augment the process peripheral regeneration. However, comprehensive elucidation complicated mechanistic foundations responsible for favorable effects nanoparticle-based therapy on regeneration remains imperative. This review aims scrutinize potential innovative carriers promoting repair. encompasses an in-depth exploration classifications synthesis methodologies associated with nanoparticles. Additionally, we discuss summarize multifaceted roles that play, including neuroprotection, facilitation axonal growth, efficient drug delivery mechanisms. Furthermore, present essential considerations highlight synergies integrating emerging technologies. Through this review, indispensable role propelling advancements Keywords: nanoparticle, nanomedicine, biomaterials, regeneration, tissue

Language: Английский

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Electrospun Scaffolds are Not Necessarily Always Made of Nanofibers as Demonstrated by Polymeric Heart Valves for Tissue Engineering

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(16)

Published: March 30, 2024

Abstract In the last 30 years, there are ≈60 000 publications about electrospun nanofibers, but it is still unclear whether nanoscale fibers really necessary for tissue engineering scaffolds. The present report puts forward this argument and reveals that compared with microfibers diameter of ≈3 µm (named as “oligo‐micro fiber”) more appropriate scaffolds owing to their better cell infiltration ability caused by larger pores available nuclear deformation. To further increase pore sizes, poly(ε‐caprolactone) (PCL) fabricated using latticed collectors meshes. Fiber orientation leads sufficient mechanical strength albeit increases porosity. exhibit good biocompatibility improve infiltration. Under aortic conditions in vitro, performances satisfactory terms acute systolic hemodynamic functionality, except higher regurgitation fraction enlarged pores. This hierarchical scaffold sparse macropores oligo‐micro filaments provides new insights into design scaffolds, may provide living heart valves regenerative capabilities patients severe valve disease future.

Language: Английский

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Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber‐Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair

Advanced Science, Journal Year: 2022, Volume and Issue: 10(4)

Published: Dec. 9, 2022

Peripheral nerve injury is a serious medical problem with limited surgical and clinical treatment options. It of great significance to integrate multiple guidance cues in one platform conduits (NGCs) promote axonal elongation functional recovery. Here, multi-functional NGC constructed regeneration by combining ordered topological structure, density gradient biomacromolecular nanoparticles, controlled delivery biological effectors provide the topographical, haptotactic, cues, respectively. On surface aligned polycaprolactone nanofibers, bioactive nanoparticles capable delivering recombinant human acidic fibroblast growth factor deposited. graded scaffold, proliferation Schwann cells promoted, directional extension neurites from both PC12 dorsal root ganglions improved direction increasing particle density. After being implanted vivo for 6 12 weeks repair 10-mm rat sciatic defect, promotes remyelination, achieving not only anatomical structure but also Taken together, provides favorable microenvironment peripheral holds promise realizing an efficacy close autograft.

Language: Английский

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Micro-nanofiber composite biomimetic conduits promote long-gap peripheral nerve regeneration in canine models

Bioactive Materials, Journal Year: 2023, Volume and Issue: 30, P. 98 - 115

Published: July 27, 2023

Peripheral nerve injuries may result in severe long-gap interruptions that are challenging to repair. Autografting is the gold standard surgical approach for repairing but can prominent donor-site complications. Instead, imitating native neural microarchitecture using synthetic conduits expected offer an alternative strategy improving regeneration. Here, we designed composed of high-resolution anisotropic microfiber grid-cordes with randomly organized nanofiber sheaths interrogate positive effects these biomimetic structures on peripheral Anisotropic microfiber-grids demonstrated capacity directionally guide Schwann cells and neurites. Nanofiber conveyed adequate elasticity permeability, whilst exhibiting a barrier function against infiltration fibroblasts. We then used composite bridge 30-mm long sciatic defects canine models. At 12 months post-implant, morphometric histological recovery, gait electrophysiological function, degree muscle atrophy were assessed. The newly regenerated tissue formed within showed restored neurological functions superior compared sheaths-only scaffolds Neurolac conduit controls. Our findings demonstrate feasibility biophysical cues effectively indicates promising clinical application prospects conduits.

Language: Английский

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Nerve Guide Conduits Integrated with Fisetin‐Loaded Chitosan Hydrogels for Reducing Oxidative Stress, Inflammation, and Nerve Regeneration

Macromolecular Bioscience, Journal Year: 2024, Volume and Issue: 24(5)

Published: Jan. 21, 2024

Peripheral nerve injuries (PNI) represent a prevalent and severe category of damage resulting from traumatic incidents. Predominantly, the deficiency in regeneration can be ascribed to enduring inflammatory reactions, hence imposing substantial clinical implications for patients. Fisetin, flavonoid derived plants, is naturally present an array vegetables fruits, including strawberries, apples, onions, cucumbers. It exhibits immunomodulatory properties through reduction inflammation oxidative stress. In research, defect addressed first time utilizing scaffold primed controlled fisetin release. this regard, fisetin-loaded chitosan hydrogels are incorporated into lumen polycaprolactone (PCL) guide conduits (NGCs). The hydrogel maintained steady release appropriate dosage. study outcomes indicated that fisetin/chitosan/polycaprolactone (FIS/CS/PCL) NGCs amplified Schwann cell proliferation neural expression, curtailed stress, alleviated inflammation, improved functions, electrophysiological properties, morphology. This pioneering has potential contribute significantly field neuroengineering.

Language: Английский

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Electroconductive and Immunomodulatory Natural Polymer‐Based Hydrogel Bandages Designed for Peripheral Nerve Regeneration

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(8)

Published: Nov. 10, 2023

Abstract Successful regeneration of the peripheral nerve relies on collaborative efforts neural cells and immune cells. Conductive hydrogels have yielded promising results in supporting axonal growth; however, their inability to regulate response poor biological integration with tissues hinder repair injured nerves. Herein, an adhesive conductive immunomodulatory hydrogel bandage is developed for regeneration. The prepared from bioactive material extracellular matrix (ECM), oxidized polysaccharides, poly(3,4‐ethylenedioxythiophene)/poly(styrenesulfonate) (PEDOT:PSS) by self‐assembly dynamic Schiff base cross‐linking. drug indole‐3‐propionic acid (IPA) loaded into bandages contributes rapid chemotaxis neutrophils dorsal root ganglia modulation system. In addition, exhibits close conformal contact nerve, forming a stable tightly coupled electrical bridge electroresponsive tissue. summary, effectively promote enable both anatomical functional recovery tissue while preventing muscle atrophy. This work provides new strategy may critical clinical applications future.

Language: Английский

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