Advanced Fluorescence Imaging Technology in the Near-Infrared-II Window for Biomedical Applications

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(35), P. 14789 - 14804

Published: Aug. 7, 2020

Fluorescence imaging has become a fundamental tool for biomedical applications; nevertheless, its intravital capacity in the conventional wavelength range (400–950 nm) been restricted by extremely limited tissue penetration. To tackle this challenge, novel approach using fluorescence second near-infrared window (NIR-II, 1000–1700 developed past decade to achieve deep penetration and high-fidelity imaging, thus significant applications have begun emerge. In Perspective, we first examine recent discoveries challenges development of NIR-II fluorophores compatible apparatuses. Subsequently, advances bioimaging, biosensing, therapy such cutting-edge technique are highlighted. Finally, based on achievement representative studies, elucidate main concerns regarding give some advice prospects future applications.

Language: Английский

---

Molecular Engineering of NIR‐II Fluorophores for Improved Biomedical Detection

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(30), P. 16294 - 16308

Published: Aug. 12, 2020

The development of fluorophores for the second near-infrared window (NIR-II, 1000-1700 nm) represents an emerging, significant, and vibrant field in analytic chemistry, chemical biology, biomedical engineering. wavelength, brightness, stability are three crucial factors that determine performance NIR-II fluorophore. Up to now, significant progress has been made fluorescence molecular probes, including synthesis D-A-D D-π-A with improved imaging construction off-on probes ratiometric via energy transfer or structure modification. In this review, we summarize most recent advances engineering design strategies then highlight a selection bioimaging biosensing applications. We also provide perspectives on potential challenges opportunities emerging field.

Language: Английский

---

J-Aggregates of Cyanine Dye for NIR-II in Vivo Dynamic Vascular Imaging beyond 1500 nm

Journal of the American Chemical Society, Journal Year: 2019, Volume and Issue: 141(49), P. 19221 - 19225

Published: Nov. 20, 2019

Light in the second near-infrared window, especially beyond 1500 nm, shows enhanced tissue transparency for high-resolution vivo optical bioimaging due to decreased scattering, absorption, and autofluorescence. Despite some inorganic luminescent nanoparticles have been developed improve around it is still a great challenge synthesize organic molecules with absorption emission toward this region. Here, we present J-aggregates 1360 nm 1370 formed by self-assembly of amphiphilic cyanine dye FD-1080 1,2-dimyristoyl-sn-glycero-3-phosphocholine. Molecular dynamics simulations were further employed illustrate process. Superior spatial resolution high signal-to-background ratio demonstrated noninvasive brain hindlimb vasculature nm. The efficacy evaluation clinically used hypotensor successfully achieved dynamic vascular imaging J-aggregates.

Language: Английский

---

Organic NIR-II molecule with long blood half-life for in vivo dynamic vascular imaging

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: June 18, 2020

Real-time monitoring of vessel dysfunction is great significance in preclinical research. Optical bioimaging the second near-infrared (NIR-II) window provides advantages including high resolution and fast feedback. However, reported molecular dyes are hampered by limited blood circulation time (~ 5-60 min) short absorption emission wavelength, which impede accurate long-term monitoring. Here, we report a NIR-II molecule (LZ-1105) with beyond 1000 nm. Thanks to long (half-life 3.2 h), fluorophore used for continuous real-time dynamic vascular processes, ischemic reperfusion hindlimbs, thrombolysis carotid artery opening recovery brain barrier (BBB). LZ-1105 an approach researchers assess due excitation wavelength properties.

Language: Английский

---

Semiconducting Polymer Nanoparticles as Theranostic System for Near-Infrared-II Fluorescence Imaging and Photothermal Therapy under Safe Laser Fluence

ACS Nano, Journal Year: 2020, Volume and Issue: 14(2), P. 2509 - 2521

Published: Feb. 5, 2020

Theranostic systems combining fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) and photothermal therapy (PTT) under safe laser fluence have great potential preclinical research clinical practice, but development of such with sufficient effective NIR-II brightness excellent properties is still challenging. Here we report a theranostic system based on semiconducting polymer nanoparticles (L1057 NPs) for PTT 980 nm irradiation, low (25 mW/cm2) high (720 fluence, respectively. Taking into consideration multiple parameters including extinction coefficient, quantum yield, portion emission region, L1057 NPs much higher than most reported organic fluorophores. The brightness, together good stability biocompatibility, allows real-time visualization whole body brain vessels detection cerebral ischemic stroke tumors clarity. maximal permissible exposure limit at allow fluence. This study demonstrates that behave as an wide range biomedical applications.

Language: Английский

---

Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time

Nature Chemistry, Journal Year: 2020, Volume and Issue: 12(12), P. 1123 - 1130

Published: Oct. 19, 2020

Language: Английский

---

Combating Concentration Quenching in Upconversion Nanoparticles

Accounts of Chemical Research, Journal Year: 2019, Volume and Issue: 53(2), P. 358 - 367

Published: Oct. 21, 2019

Lanthanide-doped upconversion nanoparticles (UCNPs) are a special class of luminescent nanomaterials that convert multiwavelength near-infrared (NIR) excitation into tunable emissions spanning the deep ultraviolet (UV) to NIR regions. In addition large anti-Stokes shift, UCNPs also feature sharp emission bandwidth, long excited-state lifetime, as well high resistance optical blinking and photobleaching. Therefore, have been identified promising candidates solve many challenging problems in fields ranging from biological imaging therapeutics photovoltaics photonics. Nevertheless, progress utilizing an process is being hindered by limited intensity, principally due low oscillator strength these nanoparticles. essentially resemble characteristics their bulk counterparts, which take advantage electronic transition within 4f configuration lanthanide dopants realize photon energy conversions. general, dopant concentration promotes luminescence providing density centers collect sustain light. However, increase induces self-quenching processes offset gain may eventually result attenuation overall intensity. This phenomenon known quenching represents major obstacle constructing bright UCNPs. recent years, advances nanoparticle research led emergence several strategies for mitigating loss at elevated concentrations. consequence, doping levels ions has become viable solution boosting intensity upconversion. On account extensive exchange interaction heavily doped UCNPs, spectrum tunability greatly enhanced. These largely expanded scope research. To provide guidelines enhancing through heavy doping, we attempt review understanding control With significant advancements made chemical synthesis, now able exquisitely various well-defined size, morphology, core-shell structure. We show that, confining transfer nanostructured host materials conjunction with innovative schemes, alleviated. As result, unusually concentrations can be used construct displaying brightness shift. demonstrate development enables advanced bioimaging photonic applications hardly fulfilled conventional comprising dopants.

Language: Английский

---

NIR-II bioluminescence for in vivo high contrast imaging and in situ ATP-mediated metastases tracing

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Aug. 21, 2020

Abstract Bioluminescence imaging has been widely used in life sciences and biomedical applications. However, conventional bioluminescence usually operates the visible region, which hampers high-performance vivo optical due to strong tissue absorption scattering. To address this challenge, here we present probes (BPs) with emission second near infrared (NIR-II) region at 1029 nm by employing resonance energy transfer (BRET) two-step fluorescence (FRET) a specially designed cyanine dye FD-1029. The biocompatible NIR-II-BPs are successfully applied vessels lymphatics mice, gives ~5 times higher signal-to-noise ratios ~1.5 spatial resolution than those obtained NIR-II imaging. Their capability of multiplexed is also well displayed. Taking advantage ATP-responding character, able recognize tumor metastasis high tumor-to-normal ratio 83.4.

Language: Английский

---

J-aggregates of meso-[2.2]paracyclophanyl-BODIPY dye for NIR-II imaging

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: April 22, 2021

Abstract J-aggregation is an efficient strategy for the development of fluorescent imaging agents in second near-infrared window. However, design J-aggregates challenging due to lack suitable dyes. Herein, we report meso -[2.2]paracyclophanyl-3,5-bis- N , -dimethylaminostyrl BODIPY (PCP-BDP2) as example dye with induced fluorescence. PCP-BDP2 shows emission maximum at 1010 nm state. Mechanism studies reveal that steric and conjugation effect PCP group on play key roles behavior photophysical properties tuning. Notably, can be utilized lymph node fluorescence-guided surgery nude mouse, which demonstrates their potential clinical application. This study alternate platform developing agents.

Language: Английский

---

The Chemistry of Organic Contrast Agents in the NIR‐II Window

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 61(14)

Published: Dec. 7, 2021

Abstract Optical imaging, especially fluorescence and photoacoustic is a non‐invasive imaging approach with high spatial temporal resolution sensitivity, compared to positron emission tomography (PET) or magnetic resonance (MRI). Due the merits of using second near‐infrared (NIR‐II) window, like deeper penetration depth, signal‐to‐noise ratio, resolution, low tissue damage, researchers have devoted great effort developing contrast agents NIR‐II absorption emission. In this Review, we summarize recently developed organic luminescent materials, ranging from small molecules conjugated polymers. Then, systematically introduce engineering strategies describe performance classified by skeleton cores. Finally, elucidate challenges prospects these dyes for potential clinical applications. We expect our summary can inspire expand spectrum diverse bioapplications.

Language: Английский

---