Advanced Fluorescence Imaging Technology in the Near-Infrared-II Window for Biomedical Applications

Journal of the American Chemical Society, Journal Year: 2020, Volume and Issue: 142(35), P. 14789 - 14804

Published: Aug. 7, 2020

Fluorescence imaging has become a fundamental tool for biomedical applications; nevertheless, its intravital capacity in the conventional wavelength range (400–950 nm) been restricted by extremely limited tissue penetration. To tackle this challenge, novel approach using fluorescence second near-infrared window (NIR-II, 1000–1700 developed past decade to achieve deep penetration and high-fidelity imaging, thus significant applications have begun emerge. In Perspective, we first examine recent discoveries challenges development of NIR-II fluorophores compatible apparatuses. Subsequently, advances bioimaging, biosensing, therapy such cutting-edge technique are highlighted. Finally, based on achievement representative studies, elucidate main concerns regarding give some advice prospects future applications.

Language: Английский

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Design of superior phototheranostic agents guided by Jablonski diagrams

Chemical Society Reviews, Journal Year: 2020, Volume and Issue: 49(22), P. 8179 - 8234

Published: Jan. 1, 2020

Phototheranostics represents a promising direction for modern precision medicine, which has recently attracted great research interest from multidisciplinary areas. Organic optical agents including small molecular fluorophores, semiconducting/conjugated polymers, aggregation-induced emission luminogens, etc. with tuneable photophysical properties, high biosafety and biocompatibility, facile processability ease of functionalization have delivered encouraging performance in disease phototheranostics. This review summarizes the recent progress organic phototheranostic an emphasis on main strategies to manipulate three excitation energy dissipation pathways, namely, radiative decay, thermal deactivation, intersystem crossing, assistance Jablonski diagram, particularly showcases how diagram been guiding design molecule aggregate levels promote outcomes. Molecular nanoengineering modulate processes convert absorbed photons into fluorescent/phosphorescent/photoacoustic signals and/or photodynamic/photothermal curing effects improved phototheranostics are elaborated. Noteworthily, adaptive activatable transformable functions demand, regulation such as chemiexcitation efficacies also included. A brief summary discussion current challenges future perspectives this field is further presented.

Language: Английский

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Molecular Engineering of NIR‐II Fluorophores for Improved Biomedical Detection

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(30), P. 16294 - 16308

Published: Aug. 12, 2020

The development of fluorophores for the second near-infrared window (NIR-II, 1000-1700 nm) represents an emerging, significant, and vibrant field in analytic chemistry, chemical biology, biomedical engineering. wavelength, brightness, stability are three crucial factors that determine performance NIR-II fluorophore. Up to now, significant progress has been made fluorescence molecular probes, including synthesis D-A-D D-π-A with improved imaging construction off-on probes ratiometric via energy transfer or structure modification. In this review, we summarize most recent advances engineering design strategies then highlight a selection bioimaging biosensing applications. We also provide perspectives on potential challenges opportunities emerging field.

Language: Английский

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Stimuli-Responsive Polymeric Nanocarriers for Drug Delivery, Imaging, and Theragnosis

Polymers, Journal Year: 2020, Volume and Issue: 12(6), P. 1397 - 1397

Published: June 22, 2020

In the past few decades, polymeric nanocarriers have been recognized as promising tools and gained attention from researchers for their potential to efficiently deliver bioactive compounds, including drugs, proteins, genes, nucleic acids, etc., in pharmaceutical biomedical applications. Remarkably, these could be further modified stimuli-responsive systems based on mechanism of triggered release, i.e., response a specific stimulus, either endogenous (pH, enzymes, temperature, redox values, hypoxia, glucose levels) or exogenous (light, magnetism, ultrasound, electrical pulses) effective biodistribution controlled release drugs genes at sites. Various nanoparticles (NPs) functionalized used templates imaging form metallic NPs, dendrimers, quantum dots, liposomes. The use active compounds has attracted considerable interest various cancer therapy fields. So-called smart nanopolymer are built respond certain stimuli such pH, light intensity wavelength, electrical, magnetic ultrasonic Many techniques explored optical imaging, resonance (MRI), nuclear photoacoustic (PAI), single photon emission computed tomography (SPECT), positron (PET). This review reports most recent developments methods by analyzing examples nanopolymers that can imaged using one more techniques. Unique features, nontoxicity, water solubility, biocompatibility, presence multiple functional groups, designate nanocues attractive nanomedicine candidates. this context, we summarize classes multifunctional, polymeric, nano-sized formulations liposomes, micelles, nanogels, dendrimers.

Language: Английский

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Versatile Types of Inorganic/Organic NIR-IIa/IIb Fluorophores: From Strategic Design toward Molecular Imaging and Theranostics

Chemical Reviews, Journal Year: 2021, Volume and Issue: 122(1), P. 209 - 268

Published: Oct. 19, 2021

In vivo imaging in the second near-infrared window (NIR-II, 1000-1700 nm), which enables us to look deeply into living subjects, is producing marvelous opportunities for biomedical research and clinical applications. Very recently, there has been an upsurge of interdisciplinary studies focusing on developing versatile types inorganic/organic fluorophores that can be used noninvasive NIR-IIa/IIb (NIR-IIa, 1300-1400 nm; NIR-IIb, 1500-1700 nm) with near-zero tissue autofluorescence deeper penetration. This review provides overview reports published date design, properties, molecular imaging, theranostics fluorophores. First, we summarize design concepts up-to-date functional biomaterials, order single-walled carbon nanotubes (SWCNTs), quantum dots (QDs), rare-earth-doped nanoparticles (RENPs), organic (OFs). Then, these novel modalities applications brought by superior fluorescent properties are reviewed. Finally, challenges perspectives future translation, aiming at boosting application progress NIR-IIa NIR-IIb technology highlighted.

Language: Английский

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Design of AIEgens for near-infrared IIb imaging through structural modulation at molecular and morphological levels

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: March 9, 2020

Abstract Fluorescence imaging in near-infrared IIb (NIR-IIb, 1500–1700 nm) spectrum holds a great promise for tissue imaging. While few inorganic NIR-IIb fluorescent probes have been reported, their organic counterparts are still rarely developed, possibly due to the shortage of efficient materials with long emission wavelength. Herein, we propose molecular design philosophy explore pure fluorophores by manipulation effects twisted intramolecular charge transfer and aggregation-induced at morphological levels. An dye emitting up 1600 nm quantum yield 11.5% NIR-II region is developed. fluorescence blood vessels deeply-located intestinal tract live mice based on dyes achieved high clarity enhanced signal-to-background ratio. We hope this study will inspire further development evolution bio-imaging.

Language: Английский

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Organic NIR-II molecule with long blood half-life for in vivo dynamic vascular imaging

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: June 18, 2020

Real-time monitoring of vessel dysfunction is great significance in preclinical research. Optical bioimaging the second near-infrared (NIR-II) window provides advantages including high resolution and fast feedback. However, reported molecular dyes are hampered by limited blood circulation time (~ 5-60 min) short absorption emission wavelength, which impede accurate long-term monitoring. Here, we report a NIR-II molecule (LZ-1105) with beyond 1000 nm. Thanks to long (half-life 3.2 h), fluorophore used for continuous real-time dynamic vascular processes, ischemic reperfusion hindlimbs, thrombolysis carotid artery opening recovery brain barrier (BBB). LZ-1105 an approach researchers assess due excitation wavelength properties.

Language: Английский

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Near-infrared luminescence high-contrast in vivo biomedical imaging

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(1), P. 60 - 78

Published: Jan. 19, 2023

Language: Английский

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Gathering brings strength: How organic aggregates boost disease phototheranostics

Aggregate, Journal Year: 2021, Volume and Issue: 2(1), P. 95 - 113

Published: Jan. 22, 2021

Abstract Phototheranostics that concurrently and complementarily integrate real‐time diagnosis in situ therapeutic capabilities one platform has become the advancing edge of precision medicine. Organic agents possess merits facile preparation, high purity, tunable photophysical property, good biocompatibility, potential biodegradability, which have shown great promise for disease theranostics. This review summarizes recent achievements organic phototheranostic applications, especially rationally utilize energy dissipation pathways Jablonski diagram to modulate fluorescence emission, photoacoustic/photothermal production, photodynamic processes. Of particular interest are systems exhibiting huge differences aggregate state as compared with solution or single molecule form, during intramolecular motions play an important role regulating properties. The advances from such aspect biomedical applications including high‐resolution imaging, activatable imaging therapy, adaptive theranostics, image‐guided surgery, immunotherapy, afterglow discussed. A brief summary perspective this field also presented. We hope will be helpful researchers interested bioprobe design theranostic inspire new insights into linkage between science field.

Language: Английский

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Perfecting and extending the near-infrared imaging window

Light Science & Applications, Journal Year: 2021, Volume and Issue: 10(1)

Published: Sept. 24, 2021

In vivo fluorescence imaging in the second near-infrared window (NIR-II) has been considered as a promising technique for visualizing mammals. However, definition of NIR-II region and mechanism accounting excellent performance still need to be perfected. Herein, we simulate photon propagation NIR (to 2340 nm), confirm positive contribution moderate light absorption by water intravital perfect 900-1880 nm, where 1400-1500 1700-1880 nm are defined NIR-IIx NIR-IIc regions, respectively. Moreover, 2080-2340 is newly proposed third (NIR-III) window, which believed provide best quality. The wide-field microscopy brain performed around region, with optical sectioning strength largest depth date. We also propose 1400 long-pass detection off-peak whose exceeds that NIR-IIb imaging, using bright fluorophores short emission wavelength.

Language: Английский

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