Journal of the American Chemical Society,
Journal Year:
2020,
Volume and Issue:
142(31), P. 13350 - 13355
Published: July 24, 2020
We
report
the
development
of
a
new
strategy
for
chemical
analysis
live
cells
based
on
protein
spherical
nucleic
acids
(ProSNAs).
The
ProSNA
architecture
enables
analyte
detection
via
highly
programmable
acid
shell
or
functional
core.
As
proof-of-concept,
we
use
an
i-motif
as
recognition
element
to
probe
pH
in
living
cells.
By
interfacing
with
forced-intercalation
readout,
introduce
quencher-free
approach
that
is
resistant
false-positive
signals,
overcoming
limitations
associated
conventional
fluorophore/quencher-based
gold
NanoFlares.
Using
glucose
oxidase
core,
show
activity-based,
amplified
sensing
glucose.
This
enzymatic
system
affords
greater
than
100-fold
fluorescence
turn
buffer,
selective
presence
close
analogs
(i.e.,
glucose-6-phosphate),
and
can
detect
above
threshold
concentration
∼5
μM,
which
study
relative
changes
intracellular
concentrations.
Angewandte Chemie International Edition,
Journal Year:
2022,
Volume and Issue:
61(19)
Published: Feb. 26, 2022
Small-molecule-based
second
near-infrared
(NIR-II)
activatable
fluorescent
probes
can
potentially
provide
a
high
target-to-background
ratio
and
deep
tissue
penetration.
However,
most
of
the
reported
NIR-II
small-molecule
exhibit
poor
versatility
owing
to
lack
general
stable
optically
tunable
group.
In
this
study,
we
designed
NIRII-HDs,
novel
dye
scaffold
optimized
for
probe
development.
particular,
NIRII-HD5
showed
best
optical
properties
such
as
proper
pKa
value,
excellent
stability,
brightness,
which
be
beneficial
in
vivo
imaging
with
contrast.
To
demonstrate
applicability
dye,
three
target-activatable
ROS,
thiols,
enzymes.
Using
these
probes,
not
only
realized
reliable
different
diseases
mouse
models
but
also
evaluated
redox
potential
liver
during
injury
fidelity.
Chemical Society Reviews,
Journal Year:
2022,
Volume and Issue:
51(3), P. 829 - 868
Published: Jan. 1, 2022
Photoacoustic
(PA)
imaging
has
emerged
as
a
powerful
technique
for
the
high
resolution
visualization
of
biological
processes
within
deep
tissue.
Through
development
and
application
exogenous
targeted
contrast
agents
activatable
probes
that
can
respond
to
given
cancer
biomarker,
researchers
image
molecular
events
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(12), P. 6596 - 6614
Published: March 13, 2023
The
use
of
two
or
more
metal
catalysts
in
a
reaction
is
powerful
synthetic
strategy
to
access
complex
targets
efficiently
and
selectively
from
simple
starting
materials.
While
capable
uniting
distinct
reactivities,
the
principles
governing
multimetallic
catalysis
are
not
always
intuitive,
making
discovery
optimization
new
reactions
challenging.
Here,
we
outline
our
perspective
on
design
elements
using
precedent
well-documented
C–C
bond-forming
reactions.
These
strategies
provide
insight
into
synergy
compatibility
individual
components
reaction.
Advantages
limitations
discussed
promote
further
development
field.
Chemical Reviews,
Journal Year:
2024,
Volume and Issue:
124(9), P. 5846 - 5929
Published: April 24, 2024
Although
transition
metals
constitute
less
than
0.1%
of
the
total
mass
within
a
human
body,
they
have
substantial
impact
on
fundamental
biological
processes
across
all
kingdoms
life.
Indeed,
these
nutrients
play
crucial
roles
in
physiological
functions
enzymes,
with
redox
properties
many
being
essential
to
their
activity.
At
same
time,
imbalances
metal
pools
can
be
detrimental
health.
Modern
analytical
techniques
are
helping
illuminate
workings
homeostasis
at
molecular
and
atomic
level,
spatial
localization
real
implications
dysregulation
disease
pathogenesis.
Fluorescence
microscopy
has
proven
one
most
promising
non-invasive
methods
for
studying
samples.
The
accuracy
sensitivity
bioimaging
experiments
predominantly
determined
by
fluorescent
metal-responsive
sensor,
highlighting
importance
rational
probe
design
such
measurements.
This
review
covers
activity-
binding-based
sensors
that
been
applied
cellular
studies.
We
focus
redox-active
metals:
iron,
copper,
manganese,
cobalt,
chromium,
nickel.
aim
encourage
further
targeted
efforts
developing
innovative
approaches
understanding
chemistry
metals.
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(5), P. 3035 - 3041
Published: Jan. 25, 2021
Inflammation
is
an
important
protection
reaction
in
living
organisms
associated
with
many
diseases.
Since
peroxynitrite
(ONOO–)
engaged
the
inflammatory
processes,
illustrating
key
nexus
between
ONOO–
and
inflammation
significant.
Due
to
lack
of
sensitive
vivo
detection
methods,
research
still
remains
at
its
infancy.
Herein,
a
highly
NIR
fluorescence
probe
DDAO-PN
for
progress
was
reported.
The
responded
significant
enhancement
657
nm
(84-fold)
within
30
s
solution.
Intracellular
imaging
exogenous
demonstrated
68-fold
increase
(F/F0).
Impressively,
can
detect
fluxes
LPS-induced
rear
leg
4.0-fold
peritonitis
8.0-fold
remarkable
quick
response
enabled
real-time
tracking
large
signal-to-noise
(S/N)
ratio.
These
results
clearly
denoted
that
able
be
high-fidelity
high
sensitivity
will
boost
inflammation-related
Proceedings of the National Academy of Sciences,
Journal Year:
2021,
Volume and Issue:
118(9)
Published: Feb. 23, 2021
Reactive
oxygen
species
(ROS)
like
hydrogen
peroxide
(H2O2)
are
transient
that
have
broad
actions
in
signaling
and
stress,
but
spatioanatomical
understanding
of
their
biology
remains
insufficient.
Here,
we
report
a
tandem
activity-based
sensing
labeling
strategy
for
H2O2
imaging
enables
capture
permanent
recording
localized
fluxes.
Peroxy
Green-1
Fluoromethyl
(PG1-FM)
is
diffusible
small-molecule
probe
senses
by
boronate
oxidation
reaction
to
trigger
dual
release
covalent
fluorescent
product,
thus
preserving
spatial
information
on
local
changes.
This
unique
reagent
visualization
transcellular
redox
microglia-neuron
coculture
cell
model,
where
selective
activation
microglia
ROS
production
increases
nearby
neurons.
In
addition
identifying
ROS-mediated
cell-to-cell
communication,
this
work
provides
starting
point
the
design
chemical
probes
can
achieve
high
fidelity
combining
strategies.
