Activity‐based Photoacoustic Probes Reveal Elevated Intestinal MGL and FAAH Activity in a Murine Model of Obesity DOI

Melissa Y. Lucero,

Sarah H. Gardner, Anuj K. Yadav

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(44)

Published: Sept. 9, 2022

Abstract Obesity is a chronic health condition characterized by the accumulation of excessive body fat which can lead to and exacerbate cardiovascular disease, type‐II diabetes, high blood pressure, cancer through systemic inflammation. Unfortunately, visualizing key mediators inflammatory response, such as monoacylglycerol lipase (MGL) fatty acid amide hydrolase (FAAH), in selective manner profound challenge owing an overlapping substrate scope that involves arachidonic (AA). Specifically, these enzymes work concert generate AA, context obesity, has been implicated control appetite energy metabolism. In this study, we developed first activity‐based sensing probes detect MGL (PA‐HD‐MGL) FAAH (PA‐HD‐FAAH) activity via photoacoustic imaging. Activation PA‐HD‐MGL PA‐HD‐FAAH their target resulted 1.74‐fold 1.59‐fold signal enhancements, respectively. Due exceptional selectivity profiles deep‐tissue imaging capabilities, were employed measure murine model obesity. Contrary conflicting reports suggesting levels be attenuated or elevated, our results support latter. Indeed, discovered marked increase both targets gastrointestinal tract. These findings set stage uncover role endocannabinoid pathway obesity‐mediated

Language: Английский

Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology DOI
Helmut Sies, Vsevolod V. Belousov, Navdeep S. Chandel

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 23(7), P. 499 - 515

Published: Feb. 21, 2022

Language: Английский

Citations

1012
Activity-based NIR fluorescent probes based on the versatile hemicyanine scaffold: design strategy, biomedical applications, and outlook DOI
Haidong Li, Heejeong Kim, Feng Xu

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(5), P. 1795 - 1835

Published: Jan. 1, 2022

The discovery of a near-infrared (NIR, 650-900 nm) fluorescent chromophore hemicyanine dye with high structural tailorability is great significance in the field detection, bioimaging, and medical therapeutic applications. It exhibits many outstanding advantages including absorption emission NIR region, tunable spectral properties, photostability as well large Stokes shift. These properties are superior to those conventional fluorogens, such coumarin, fluorescein, naphthalimides, rhodamine, cyanine. Researchers have made remarkable progress developing activity-based multifunctional probes based on skeletons for monitoring vital biomolecules living systems through output fluorescence/photoacoustic signals, integration diagnosis treatment diseases using chemotherapy or photothermal/photodynamic therapy combination therapy. achievements prompted researchers develop more smart fluorogen template. In this review, we begin by describing brief history dyes, synthetic approaches, design strategies functional probes. Then, selected hemicyanine-based that can detect ions, small biomolecules, overexpressed enzymes diagnostic reagents systematically highlighted. Finally, potential drawbacks outlook future investigation clinical medicine transformation activatable also discussed.

Language: Английский

Citations

341
Fluorescent probes for the detection of disease-associated biomarkers DOI
Wei‐Tao Dou, Hai‐Hao Han, Adam C. Sedgwick

et al.

Science Bulletin, Journal Year: 2022, Volume and Issue: 67(8), P. 853 - 878

Published: Jan. 17, 2022

Language: Английский

Citations

185
HPCA1 is required for systemic reactive oxygen species and calcium cell-to-cell signaling and plant acclimation to stress DOI Open Access
Yosef Fichman, Sara I. Zandalinas, Scott C. Peck

et al.

The Plant Cell, Journal Year: 2022, Volume and Issue: 34(11), P. 4453 - 4471

Published: Aug. 4, 2022

Abstract Reactive oxygen species (ROS), produced by respiratory burst oxidase homologs (RBOHs) at the apoplast, play a key role in local and systemic cell-to-cell signaling, required for plant acclimation to stress. Here we reveal that Arabidopsis thaliana leucine-rich-repeat receptor-like kinase H2O2-INDUCED CA2+ INCREASES 1 (HPCA1) acts as central ROS receptor propagation of signals, signaling response different biotic abiotic stresses, stress responses tissues, stress, following treatment high light (HL) We further report HPCA1 is calcium but not membrane depolarization responses, identify calcium-permeable channel MECHANOSENSITIVE ION CHANNEL LIKE 3, CALCINEURIN B-LIKE CALCIUM SENSOR 4 (CBL4), CBL4-INTERACTING PROTEIN KINASE 26 Sucrose-non-fermenting-1-related Protein Kinase 2.6/OPEN STOMATA (OST1) signals. In addition, serine residues S343 S347 RBOHD (the putative targets OST1) playing application HL Our findings plays mediating coordinating signals

Language: Английский

Citations

99
Unveiling the Crucial Roles of O2•–and ATP in Hepatic Ischemia–Reperfusion Injury Using Dual-Color/Reversible Fluorescence Imaging DOI Creative Commons
Jihong Liu, Wen Zhang, Xin Wang

et al.

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(36), P. 19662 - 19675

Published: Sept. 1, 2023

Hepatic ischemia-reperfusion injury (HIRI) is mainly responsible for morbidity or death due to graft rejection after liver transplantation. During HIRI, superoxide anion (O2•-) and adenosine-5'-triphosphate (ATP) have been identified as pivotal biomarkers associated with oxidative stress energy metabolism, respectively. However, how the temporal spatial fluctuations of O2•- ATP coordinate changes in HIRI particularly they synergistically regulate each other pathological mechanism remains unclear. Herein, we rationally designed successfully synthesized a dual-color dual-reversible molecular fluorescent probe (UDP) dynamic simultaneous visualization real-time, uncovered their interrelationship synergy HIRI. UDP featured excellent sensitivity, selectivity, reversibility response ATP, which rendered suitable detecting generating independent responses blue red fluorescence channels without spectral crosstalk. Notably, situ imaging revealed first time synchronous bursts depletion hepatocytes mouse livers during process Surprisingly, slight increase was observed reperfusion. More importantly, intracellular O2•-─succinate dehydrogenase (SDH)─mitochondrial (Mito) reduced nicotinamide adenine dinucleotide (NADH)─Mito ATP─intracellular cascade signaling pathway unveiled illustrated correlation between time. This research confirms potential monitoring provides clear illustration pathogenesis.

Language: Английский

Citations

60
A General Approach to Convert Hemicyanine Dyes into Highly Optimized Photoacoustic Scaffolds for Analyte Sensing** DOI
Sarah H. Gardner, Catharine Brady, Cameron Keeton

et al.

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(34), P. 18860 - 18866

Published: June 5, 2021

Most photoacoustic (PA) imaging agents are based on the repurposing of existing fluorescent dye platforms that exhibit non-optimal properties for PA applications. Herein, we introduce PA-HD, a new scaffold optimized probe development features 4.8-fold increase in sensitivity and red-shift λabs from 690 nm to 745 enable ratiometric imaging. Computational modeling was used elucidate origin these enhanced properties. To demonstrate generalizability our remodeling efforts, developed three probes β-galactosidase activity (PA-HD-Gal), nitroreductase (PA-HD-NTR), H2 O2 (PA-HD-H2 ). We generated two cancer models evaluate PA-HD-Gal PA-HD-NTR. employed murine model Alzheimer's disease test PA-HD-H2 . There, observed signal at 735 1.79±0.20-fold relative background, indicating presence oxidative stress. These results were confirmed via calibration, which not possible using parent HD platform.

Language: Английский

Citations

98
Design of an HPPD fluorescent probe and visualization of plant responses to abiotic stress DOI Creative Commons

Xiaoyan Zeng,

Yurou Huang,

Jing Dong

et al.

Advanced Agrochem, Journal Year: 2022, Volume and Issue: 1(1), P. 73 - 84

Published: Aug. 11, 2022

The upregulation of 4-hydroxyphenylpyruvate dioxygenase (HPPD) can protect plants from adverse abiotic stress. Therefore, studying the changes HPPD under stress is extremely valuable. In this study, we employed a rational molecular design strategy to prepare an HPPD-responsive fluorescent probe consisting pyrene fluorophore, linker and benquitrione skeleton recognition moiety that functions via aggregation–disaggregation sensing mechanism. As predicted, exhibited obvious turn-on fluorescence response towards HPPD. addition, in vivo imaging demonstrated excellent capability track Arabidopsis thaliana, dynamic were monitored different degrees high temperature Cadmium (II) ion (Cd2+) This work provides efficient for acquiring noninvasive probe, which could evaluate degree be further exploring resistance mechanism plants.

Language: Английский

Citations

62
Antigen-Specific T Cell Detection via Photocatalytic Proximity Cell Labeling (PhoXCELL) DOI
Hongyu Liu,

Huixin Luo,

Qi Xue

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(12), P. 5517 - 5526

Published: March 21, 2022

Quantitative detection and characterization of antigen-specific T cells are crucial to our understanding immune responses as well the development new immunotherapies. Herein, we report a spatiotemporally resolved method for quantification cell–cell interactions via Photocatalytic proXimity CELl Labeling (PhoXCELL). The biocompatible photosensitizer dibromofluorescein (DBF) was leveraged optimized nongenetic alternative enzymatic approaches efficient generation singlet oxygen upon photoirradiation (520 nm) on cell surface, which allowed subsequent labeling nearby oxidized proteins with primary aliphatic amine-based probes. We demonstrated that DBF-functionalized dendritic (DCs) could label interacting in synapses rapid quantitatively discriminated interaction strength, revealed distinct gene signatures strongly interact antigen-pulsed DCs. Furthermore, employed PhoXCELL simultaneously detect tumor CD8+ CD4+ from tumor-infiltrating lymphocytes draining lymph nodes murine models, enabling powerful platform identify receptor (TCR)-relevant personal immunotherapy.

Language: Английский

Citations

52
Methionine oxidation activates pyruvate kinase M2 to promote pancreatic cancer metastasis DOI Creative Commons
Dan He,

Huijin Feng,

Belen Sundberg

et al.

Molecular Cell, Journal Year: 2022, Volume and Issue: 82(16), P. 3045 - 3060.e11

Published: June 24, 2022

Language: Английский

Citations

46
Enlarging the Stokes Shift by Weakening the π‐Conjugation of Cyanines for High Signal‐to‐Noise Ratiometric Imaging DOI
Yongkang Yue, Tingting Zhao, Xu Zhou

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 10(3)

Published: Nov. 24, 2022

The signal-to-noise ratio (SNR) is one of the key features a fluorescent probe and that often defines its potential utility for in vivo labeling analyte detection applications. Here, it reported introducing pyridine group into traditional cyanine-7 dyes an asymmetric manner provides series tunable NIR (Cy-Mu-7) characterized by enhanced Stokes shifts (≈230 nm) compared to parent cyanine 7 dye (<25 nm). observed shift increase ascribed symmetry breaking Cy-Mu-7 core reduction extent conjugation. fluorescence signals are upon confinement within hydrophobic cavity albumin or via spontaneous encapsulation micelles aqueous media. Utilizing Cy-Mu-7, ultra-fast kidney mice realized, found liver injury will aggravate burden monitoring intensity liver. In addition, could be used as efficient chemiluminescence resonance energy transfer acceptor reaction between H

Language: Английский

Citations

39