Rational Design of MMP-Independent Near-Infrared Fluorescent Probes for Accurately Monitoring Mitochondrial Viscosity DOI
Xue Wu, Ruixin Zhang, Ying Li

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(19), P. 7611 - 7619

Published: May 3, 2023

Mitochondrial viscosity affects metabolite diffusion and mitochondrial metabolism is associated with many diseases. However, the accuracy of mitochondria-targeting fluorescent probes in measuring unsatisfactory because these can diffuse from mitochondria during mitophagy a decreased membrane potential (MMP). To avoid this problem, by incorporating different alkyl side chains into dihydroxanthene fluorophores (denoted as DHX), we developed six near-infrared (NIR) for accurate detection viscosity, sensitivity to targeting anchoring capability increased increasing chain length. Among them, DHX-V-C12 had highly selective response variations minimum interference polarity, pH, other biologically relevant species. Furthermore, was used monitor changes HeLa cells treated ionophores (nystatin, monensin) or under starvation conditions. We hope that strategy based on length will be general analytes, enabling study functions.

Language: Английский

Near-infrared (NIR) fluorescence-emitting small organic molecules for cancer imaging and therapy DOI
Hui Li, Yujun Kim,

Hyoje Jung

et al.

Chemical Society Reviews, Journal Year: 2022, Volume and Issue: 51(21), P. 8957 - 9008

Published: Jan. 1, 2022

We discuss recent advances made in the development of NIR fluorescence-emitting small organic molecules for tumor imaging and therapy.

Language: Английский

Citations

223
The Chemistry of Organic Contrast Agents in the NIR‐II Window DOI
Jing Mu, Ming Xiao, Yu Shi

et al.

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 61(14)

Published: Dec. 7, 2021

Abstract Optical imaging, especially fluorescence and photoacoustic is a non‐invasive imaging approach with high spatial temporal resolution sensitivity, compared to positron emission tomography (PET) or magnetic resonance (MRI). Due the merits of using second near‐infrared (NIR‐II) window, like deeper penetration depth, signal‐to‐noise ratio, resolution, low tissue damage, researchers have devoted great effort developing contrast agents NIR‐II absorption emission. In this Review, we summarize recently developed organic luminescent materials, ranging from small molecules conjugated polymers. Then, systematically introduce engineering strategies describe performance classified by skeleton cores. Finally, elucidate challenges prospects these dyes for potential clinical applications. We expect our summary can inspire expand spectrum diverse bioapplications.

Language: Английский

Citations

219
Activatable molecular probes for fluorescence-guided surgery, endoscopy and tissue biopsy DOI
Yan Zhang, Guopeng Zhang,

Ziling Zeng

et al.

Chemical Society Reviews, Journal Year: 2021, Volume and Issue: 51(2), P. 566 - 593

Published: Dec. 20, 2021

The real-time, dynamic optical visualization of lesions and margins ensures not only complete resection the malignant tissues but also better preservation vital organs/tissues during surgical procedures. Most imaging probes with an "always-on" signal encounter high background noise due to their non-specific accumulation in normal tissues. By contrast, activatable molecular "turn on" signals upon reaction targeted biomolecules that are overexpressed cells, offering target-to-background ratios specificity sensitivity. This review summarizes recent progress diagnosis. design principle mechanism discussed, followed by specific emphasis on applications ranging from fluorescence-guided surgery endoscopy tissue biopsy. Finally, potential challenges perspectives field probe-enabled discussed.

Language: Английский

Citations

172
Chemical Design of Activatable Photoacoustic Probes for Precise Biomedical Applications DOI

Yongchao Liu,

Lili Teng,

Baoli Yin

et al.

Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(6), P. 6850 - 6918

Published: March 2, 2022

Photoacoustic (PA) imaging technology, a three-dimensional hybrid modality that integrates the advantage of optical and acoustic imaging, has great application prospects in molecular due to its high depth resolution. To endow PA with ability for real-time visualization precise biomedical diagnosis, numerous activatable probes which can specifically alter their intensities upon reacting targets or biological events interest have been developed. This review highlights recent developments applications including detection biotargets events. First, generation mechanism signals will be given, followed by brief introduction contrast agents used probe design. Then we particularly summarize general design principles alteration strategies developing probes. Furthermore, give detailed discussion living systems. At last, current challenges outlooks future discussed. We hope this stimulate new ideas explore potentials future.

Language: Английский

Citations

167
Fluorescent dyes based on rhodamine derivatives for bioimaging and therapeutics: recent progress, challenges, and prospects DOI

Shuang Zeng,

Xiaosheng Liu, Yves S. Kafuti

et al.

Chemical Society Reviews, Journal Year: 2023, Volume and Issue: 52(16), P. 5607 - 5651

Published: Jan. 1, 2023

Since their inception, rhodamine dyes have been extensively applied in biotechnology as fluorescent markers or for the detection of biomolecules owing to good optical physical properties. Accordingly, they emerged a powerful tool visualization living systems. In addition fluorescence bioimaging, molecular design derivatives with disease therapeutic functions (e.g., cancer and bacterial infection) has recently attracted increased research attention, which is significantly important construction libraries diagnostic integration. However, reviews focusing on integrated strategies dye-based diagnosis treatment wide application are extremely rare. this review, first, brief history development dyes, transformation from bioimaging therapy, concept optics-based integration its significance human presented. Next, systematic review several excellent rhodamine-based well treatment, Finally, challenges practical future outlook clinical translation also discussed.

Language: Английский

Citations

158
A Dual‐Locked Activatable Phototheranostic Probe for Biomarker‐Regulated Photodynamic and Photothermal Cancer Therapy DOI
Xin Wei, Chi Zhang, Shasha He

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(26)

Published: April 9, 2022

Activatable phototheranostics holds promise for precision cancer treatment owing to the "turn-on" signals and therapeutic effects. However, most activatable phototheranostic probes only possess photodynamic therapy (PDT) or photothermal (PTT), which suffer from poor efficacy due deficient cellular oxygen complex tumor microenvironment. We herein report a dual-locked probe that activates near-infrared fluorescence (NIRF) in tumor, triggers PDT response tumor-periphery biomarker, switches PTT upon detecting tumor-core-hypoxia biomarker. This PDT-PTT auto-regulated exhibits complete ablation under photoirradiation of single laser source by producing cytotoxic singlet at periphery generating hyperthermia tumor-core where is too hypoxic PDT. represents promising molecular design approach toward precise phototheranostics.

Language: Английский

Citations

127
Near infrared fluorescent probes for detecting and imaging active small molecules DOI

Caiping Ding,

Tian‐Bing Ren

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 482, P. 215080 - 215080

Published: Feb. 21, 2023

Language: Английский

Citations

127
Acceptor engineering for NIR-II dyes with high photochemical and biomedical performance DOI Creative Commons

Aiyan Ji,

Hongyue Lou,

Chunrong Qu

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: July 2, 2022

It is highly important and challenging to develop donor-acceptor-donor structured small-molecule second near-infrared window (NIR-II) dyes with excellent properties such as water-solubility chem/photostability. Here, we discovery an electron acceptor, 6,7-di(thiophen-2-yl)-[1,2,5]thiadiazolo[3,4-g]quinoxaline (TQT) highest stability in alkaline conditions, compared conventional NIR-II building block benzobisthiadiazole (BBT) 6,7-diphenyl-[1,2,5] thiadiazolo[3,4-g]quinoxaline (PTQ). The sulfonated hydrophilic dye, FT-TQT, further synthesized 2.13-fold increased quantum yield than its counterpart FT-BBT BBT acceptor. FT-TQT complexed FBS also prepared displays a 16-fold increase fluorescence intensity alone. demonstrates real-time cerebral tumor vessel imaging capability µm-scale resolution. Dynamic monitoring of vascular disruption after drug treatment achieved by fluorescent imaging. Overall, TQT efficient acceptor for designing innovative dyes. engineering strategy provides promising approach design next generation fluorophores which open new biomedical applications.

Language: Английский

Citations

114
Engineering of BODIPY-based theranostics for cancer therapy DOI
Zhiqiang Mao, Ji Hyeon Kim, Jieun Lee

et al.

Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 476, P. 214908 - 214908

Published: Nov. 7, 2022

Language: Английский

Citations

101
NIRII‐HDs: A Versatile Platform for Developing Activatable NIR‐II Fluorogenic Probes for Reliable In Vivo Analyte Sensing DOI

Zuojia Qin,

Tian‐Bing Ren, Huijie Zhou

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(19)

Published: Feb. 26, 2022

Small-molecule-based second near-infrared (NIR-II) activatable fluorescent probes can potentially provide a high target-to-background ratio and deep tissue penetration. However, most of the reported NIR-II small-molecule exhibit poor versatility owing to lack general stable optically tunable group. In this study, we designed NIRII-HDs, novel dye scaffold optimized for probe development. particular, NIRII-HD5 showed best optical properties such as proper pKa value, excellent stability, brightness, which be beneficial in vivo imaging with contrast. To demonstrate applicability dye, three target-activatable ROS, thiols, enzymes. Using these probes, not only realized reliable different diseases mouse models but also evaluated redox potential liver during injury fidelity.

Language: Английский

Citations

98