Autocatalytic DNA circuitries DOI
Qiong Wu,

Wei Xu,

Jinhua Shang

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(22), P. 10878 - 10899

Published: Jan. 1, 2024

Autocatalysis, a self-sustained replication process where at least one of the products functions as catalyst, plays pivotal role in life's evolution, from genome duplication to emergence autocatalytic subnetworks cell division and metabolism. Leveraging their programmability, controllability, rich functionalities, DNA molecules have become cornerstone for engineering circuits, driving diverse technological applications. In this tutorial review, we offer comprehensive survey recent advances circuits practical implementations. We delve into fundamental principles underlying construction these highlighting reliance on DNAzyme biocatalysis, enzymatic catalysis, dynamic hybridization assembly. The discussed circuitry techniques revolutionized ultrasensitive sensing biologically significant molecules, encompassing genomic DNAs, RNAs, viruses, proteins. Furthermore, amplicons produced by serve building blocks higher-order nanostructures, facilitating biomimetic behaviors such high-performance intracellular bioimaging precise algorithmic summarize applications extensively address current challenges, potential solutions, future trajectories circuits. This review promises novel insights advancement utilization across bioanalysis, biomedicine, biomimetics.

Language: Английский

DNA Tetrahedra as Functional Nanostructures: From Basic Principles to Applications DOI Creative Commons
Yu Ouyang, Pu Zhang, Itamar Willner

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(41)

Published: July 22, 2024

Abstract Self‐assembled supramolecular DNA tetrahedra composed of programmed sequence‐engineered complementary base‐paired strands represent elusive nanostructures having key contributions to the development and diverse applications nanotechnology. By appropriate engineering strands, tuneable sizes chemical functionalities were designed. Programmed for integrated into structures including sequence‐specific recognition (aptamers), catalytic DNAzymes, nanoparticles, proteins, or fluorophore. The article presents a comprehensive review addressing methods assemble characterize nanostructures, framework are discussed. Topics being addressed include application structurally functionalized nanostructure assembly optical electrochemical sensing platforms intracellular imaging modules. In addition, triggered reconfiguration dynamic networks circuits emulating biological transformations introduced. Moreover, functionalization frameworks with nanoparticles provides building units devices crystallization nanoparticle superlattices. Finally, in field nanomedicine addressed. These tetrahedra‐assisted permeation nanocarriers cells imaging, controlled drug release, active chemodynamic/photodynamic treatment target tissues, regenerative medicine.

Language: Английский

Citations

10
The Evolution of Nucleic Acid Nanotechnology: From DNA Assembly to DNA‐Encoded Library DOI Open Access
Qigui Nie, Xianfu Fang,

Jiale Huang

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 13, 2025

Abstract Deoxyribonucleic acid (DNA), a fundamental biomacromolecule in living organisms, serves as the carrier of genetic information. Beyond its role encoding biological functions, DNA's inherent ability to hybridize through base pairing has opened new avenues for application sciences. This review introduces DNA nanotechnology and DNA‐encoded library (DEL), highlights their shared design principles related assembly. First, foundational overview structural nanotechnology, including strategies historical development is provided. Subsequently, various approaches are examined dynamic from strand displacement reactions DNA‐templated polymer synthesis. Second, how principle assembly facilitated diverse formats self‐assembly‐based DEL synthesis, DNA‐template (DTS), template‐mediated proximity induction effects examined. These advancements all underpinned by unique property Finally, this summarizes common terms methodology design. Additionally, potential synergies explored between these two technologies, envisioning future applications where they can be combined create more versatile exquisite functionalities.

Language: Английский

Citations

1
External stimulation-controlled dynamic DNA devices for biosensing and biomedical applications DOI

Wei Xu,

Feifei Hu, Jiajing Li

et al.

Science China Chemistry, Journal Year: 2023, Volume and Issue: 66(11), P. 3105 - 3115

Published: Oct. 7, 2023

Language: Английский

Citations

18
Synthetic DNA-based Swimmers Driven by Enzyme Catalysis DOI

Tania Patiño Padial,

Erica Del Grosso,

Serena Gentile

et al.

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(18), P. 12664 - 12671

Published: April 8, 2024

Here, we report DNA-based synthetic nanostructures decorated with enzymes (hereafter referred to as DNA–enzyme swimmers) that self-propel by converting the enzymatic substrate product in solution. The swimmers are obtained from tubular DNA structures self-assemble spontaneously hybridization of tiles. We functionalize these two different enzymes, urease and catalase, show they exhibit concentration-dependent movement enhanced diffusion upon addition (i.e., urea H2O2). To demonstrate programmability such swimmers, also engineer strands displace enzyme scaffold, thus acting molecular "brakes" on swimmers. These results serve a first proof principle for development enzyme-powered can fluids.

Language: Английский

Citations

6
A facile DNA coacervate platform for engineering wetting, engulfment, fusion and transient behavior DOI Creative Commons
Wei Liu, Jie Deng,

Siyu Song

et al.

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: May 1, 2024

Biomolecular coacervates are emerging models to understand biological systems and important building blocks for designer applications. DNA can be used build up programmable coacervates, but often the processes make those only available specialists. Here, we report a simple approach formation of dynamic, multivalency-driven using long single-stranded homopolymer in combination with series palindromic binders serve as synthetic coacervate droplet. We reveal details on how length sequence multivalent influence formation, introduce switching autonomous behavior reaction circuits, well engineer wetting, engulfment fusion multi-coacervate system. Our simple-to-use model enhance understanding dynamics, fusion, phase transition mechanisms, wetting between forming solid foundation development innovative fundamental studies

Language: Английский

Citations

5
Dynamic Gold Nanostructures Based on DNA Self Assembly DOI
Bo Kou, Zhichao Wang,

Shikufa Mousavi

et al.

Small, Journal Year: 2023, Volume and Issue: 20(23)

Published: Dec. 24, 2023

Abstract The combination of DNA nanotechnology and Nano Gold (NG) plasmon has opened exciting possibilities for a new generation functional plasmonic systems that exhibit tailored optical properties find utility in various applications. In this review, the booming development dynamic gold nanostructures are summarized, which formed by self‐assembly using DNA‐modified NG, frameworks, driving forces. utilization bottom‐up strategies enables precise control over assembly reversible aggregations, nano‐switcher structures, robotic nanomachines capable undergoing on‐demand, structural changes profoundly impact their properties. Benefiting from vast design possibilities, complete addressability, sub‐10 nm resolution, duplexes, tiles, single‐stranded tiles origami structures serve as excellent platforms constructing diverse 3D reconfigurable with Leveraging responsive nature interactions, fabrication assemblies NG becomes readily achievable, environmental stimulation can be harnessed force nanomotors. It is envisioned intelligent DNA‐assembled nanodevices will assume increasingly important roles realms biological, biomedical, nanomechanical studies, opening avenue toward exploration innovation.

Language: Английский

Citations

11
Monodispersed and Monofunctionalized DNA‐Caged Au Nano‐Clusters with Enhanced Optical Properties for STED Imaging DOI

Liqing Qi,

Yating Xiao,

Xiaoyi Fu

et al.

Small, Journal Year: 2024, Volume and Issue: 20(29)

Published: Feb. 22, 2024

The performance of Stimulated Emission Depletion (STED) microscopy depends critically on the fluorescent probe. Ultrasmall Au nanoclusters (Au NCs) exhibit large Stokes shift, and good stimulated emission response, which are potentially useful for STED imaging. However, NCs polydispersed in size, sensitive to surrounding environment, difficult control surface functional group stoichiometry, results reduced density high heterogeneity labeling biological structures. Here, this limitation is overcome by developing a method encapsulate ultrasmall with DNA cages, yielded monodispersed, monofunctionalized that long-term stable. Moreover, DNA-caging also greatly improved fluorescence quantum yield photostability NCs. In imaging, DNA-caged ≈40 nm spatial resolution able resolve microtubule line shapes homogeneity. contrast, without caging, NCs-DNA conjugates only achieved ≈55 spotted, poorly resolved structures, due presence aggregates. Overall, developed achieve precise functionalization improve monodispersity, stability, as well optical properties NCs, providing promising class probes

Language: Английский

Citations

4
DNA polymer conjugates: Revolutionizing neurological disorder treatment through targeted drug delivery DOI Creative Commons
Pratikeswar Panda,

Swapna Sudhir Kar,

Rajaram Mohapatra

et al.

Next Materials, Journal Year: 2024, Volume and Issue: 7, P. 100336 - 100336

Published: Aug. 3, 2024

Language: Английский

Citations

4
Folding Competition and Dynamic Transformation in DNA Origami: Parallel Versus Antiparallel Crossovers DOI Creative Commons
Jung Yeon Lee, Qi Yang, Xu Chang

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract DNA is a versatile abiomaterial for constructing nanostructures with biomedical and biotechnological applications. Among the methods available, origami robust widely recognized technique. Traditionally, most designs adopt antiparallel crossovers in both scaffold staple strands, less emphasis on parallel crossovers, which offer advantages like enhanced nuclease resistance single‐strand routing potential. Here, nanostructure designed, featuring two rotational panels that can be locked into configurations based either or crossovers. By systematically varying length arrangement of these key staples, 36 pairs are studied competitive folding tests, providing insights relative preference each design. The 12 ranked, their pathways examined, assessed. results reveal staples near central crossover crucial shifting between conformations. Additionally, two‐way isothermal transformation driven by toehold‐mediated displacement reactions demonstrated, highlighting potential as dynamic nanodevices temperature‐sensitive environments. This study offers valuable ‐ dynamics origami, opening opportunities designing

Language: Английский

Citations

0
DNA‑Directed Assembly of Photonic Nanomaterials for Diagnostic and Therapeutic Applications DOI Creative Commons
Longjiang Ding, Bing Liu, Andreas Peil

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Abstract DNA‐directed assembly has emerged as a versatile and powerful approach for constructing complex structured materials. By leveraging the programmability of DNA nanotechnology, highly organized photonic systems can be developed to optimize light‐matter interactions improved diagnostics therapeutic outcomes. These enable precise spatial arrangement components, minimizing material usage, simplifying fabrication processes. nanostructures, such origami, provide robust platform building multifunctional devices with tailored optical properties. This review highlights recent progress in nanomaterials, focusing on their applications therapeutics. It provides an overview latest advancements field, discussing principles assembly, strategies functionalizing blocks, innovations design, resulting effects that drive these developments. The also explores how architectures contribute diagnostic applications, emphasizing potential create efficient effective specific healthcare needs.

Language: Английский

Citations

0