Science China Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 21, 2024
Language: Английский
Science China Chemistry, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 5, 2024
Language: Английский
Citations
17
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(26)
Published: Feb. 22, 2024
Abstract Synthetic biochemical circuits (e.g., DNA circuits) remain at the forefront of intracellular biosensing tasks yet are hindered by undesired off‐site activation and accompanying signal leakage. Herein, study attempts to overcome this limitation developing a simple‐yet‐powerful endogenous glutathione (GSH)‐regulating tactic that permits robust distinguishable on‐site microRNA (miRNA) imaging under disturbed redox homeostasis. Specifically, hierarchically activated catalytic (HAD) circuit is fabricated grafting disulfide linkage within entropy‐driven circuitry (EDC) reactants. It exemplified HAD system promises spatiotemporally selective microRNA‐21 (miR‐21) in living cells differentiation tumor from normal cells. The correlationship between GSH miRNA extensively explored live cells, can substantially expand toolbox for profiling processes.
Language: Английский
Citations
10
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(23), P. 9666 - 9675
Published: May 30, 2024
Epigenetic modification plays an indispensable role in regulating routine molecular signaling pathways, yet it is rarely used to modulate self-assembly networks. Herein, we constructed a bioorthogonal demethylase-stimulated DNA circuitry (DSC) system for high-fidelity imaging of microRNA (miRNA) live cells and mice by eliminating undesired off-site signal leakage. The simple robust DSC composed primary cell-specific regulation (CR) module ultimate signal-transducing amplifier (SA) module. After the modularly designed was delivered into target cells, DNAzyme CR site-specifically activated endogenous demethylase produce fuel strands subsequent miRNA-targeting SA Through on-site multiply guaranteed recognitions, lucid efficient realized reliably amplified vivo miRNA sensing enabled in-depth exploration demethylase-involved pathway with cells. Our bioorthogonally on-site-activated represents universal versatile biomolecular platform via various regulations shows more prospects different personalized theragnostics.
Language: Английский
Citations
10
Science China Chemistry, Journal Year: 2024, Volume and Issue: 67(7), P. 2412 - 2422
Published: June 13, 2024
Language: Английский
Citations
7
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(31), P. 12854 - 12861
Published: July 23, 2024
Sensitive and reliable microRNA imaging in living cells has significant implications for clinical diagnosis monitoring. Catalytic DNA circuits have emerged as potent tools tracking these intracellular biomarkers probing the corresponding biochemical processes. However, their utility is hindered by low resistance to external interference, leading undesired off-site activation consequent signal leakage. Therefore, achieving endogenous control of circuit's preferable target analysis cells. In this study, we attempted address challenge engineering a simple yet effective glutathione (GSH)-regulated hybridization chain reaction (HCR) circuit acquiring high-contrast miRNA imaging. Initially, HCR hairpin reactants were blocked engineered disulfide-integrated duplex, thus effectively passivating sensing function. And precaged was liberated cell-specific GSH molecule, initiating system selectively amplified detection microRNA-21 (miR-21). This approach prevented unwanted leakage before exposure into cells, ensuring robust miR-21 with high accuracy reliability specific tumor Moreover, endogenously responsive established link between small regulatory factors miRNA, thereby enhancing gain. summary, activatable represents versatile toolbox bioanalysis exploration potential signaling pathways
Language: Английский
Citations
5
Small, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 16, 2024
Abstract Artificial DNA circuits represent a versatile yet promising toolbox for in situ monitoring and concomitant regulation of diverse biological events within live cells. Nonetheless, their performance is significantly impeded by the diffusion‐dominated slow reaction kinetics uncontrollable off‐target activation. Herein, self‐localized cascade (SLC) circuit reported robust efficient microRNA (miRNA) analysis living The SLC consists cell‐specific localization module analyte‐specific signal amplification module. By integrating probes these two modules, complexity system reduced to realize responsive co‐localization circuitry simultaneous amplification. Taking advantage specifically activated, self‐localized, design, successfully achieves miRNA‐21 (miR‐21) imaging accurate cells differentiation. Moreover, reverse mechanism explored between messenger RNA (mRNA) miRNA through engineered further elucidates underlying signaling pathways them. Therefore, provides powerful tool sensitive detection intracellular biomolecules study corresponding cell regulatory mechanisms.
Language: Английский
Citations
4
Current Applied Physics, Journal Year: 2024, Volume and Issue: 67, P. 1 - 17
Published: July 14, 2024
Language: Английский
Citations
3
Analytical Methods, Journal Year: 2024, Volume and Issue: 16(36), P. 6079 - 6097
Published: Jan. 1, 2024
Nonenzymatic biosensors for sports wearables face challenges in sensitivity & specificity. Antigen–antibody, aptamer, molecular imprinting strategies are explored, enhancing performance via probes, nanostructures, multi-targets, microfluidics.
Language: Английский
Citations
3
Science China Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 6, 2025
Language: Английский
Citations
0
Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117404 - 117404
Published: March 1, 2025
Language: Английский
Citations
0