Unusual phototransduction via cross-motif signaling from G q to adenylyl cyclase in intrinsically photosensitive retinalganglion cells DOI Creative Commons
Lujing Chen, Guang Li,

Zheng Jiang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 120(1)

Published: Dec. 29, 2022

Nonimage-forming vision in mammals is mediated primarily by melanopsin (OPN4)-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs). In mouse M1-ipRGCs, predominantly activates, via Gαq,11,14, phospholipase C-β4 to open transient receptor 6 (TRPC6) and TRPC7 channels. M2- M4-ipRGCs, however, a prominent phototransduction mechanism involves the opening of hyperpolarization- cyclic nucleotide-gated channels nucleotide, although upstream steps remain uncertain. We report here experiments, on with photo-uncaging nucleotides virally expressed CNGA2 conclude that second messenger adenosine monophosphate (cAMP) - very surprising considering guanosine (cGMP) used almost all nucleotide-mediated mechanisms across animal kingdom. further found G protein likewise Gq, which its Gβγ subunits directly activates adenylyl cyclase (AC). Our findings are demonstration native cell cross-motif GPCR signaling pathway from Gq AC specific function.

Language: Английский

Single-Cell Profiles of Retinal Ganglion Cells Differing in Resilience to Injury Reveal Neuroprotective Genes DOI Creative Commons
Nicholas M. Tran, Karthik Shekhar,

Irene E. Whitney

et al.

Neuron, Journal Year: 2019, Volume and Issue: 104(6), P. 1039 - 1055.e12

Published: Nov. 26, 2019

Language: Английский

Citations

534
Melanopsin and the Intrinsically Photosensitive Retinal Ganglion Cells: Biophysics to Behavior DOI Creative Commons
Michael Tri H.

Neuron, Journal Year: 2019, Volume and Issue: 104(2), P. 205 - 226

Published: Oct. 1, 2019

Language: Английский

Citations

243
A Visual Circuit Related to Habenula Underlies the Antidepressive Effects of Light Therapy DOI Creative Commons
Lu Huang, Yue Xi,

Yanfang Peng

et al.

Neuron, Journal Year: 2019, Volume and Issue: 102(1), P. 128 - 142.e8

Published: Feb. 19, 2019

Language: Английский

Citations

241
Diversity of intrinsically photosensitive retinal ganglion cells: circuits and functions DOI
Marcos L. Aranda, Tiffany M. Schmidt

Cellular and Molecular Life Sciences, Journal Year: 2020, Volume and Issue: 78(3), P. 889 - 907

Published: Sept. 23, 2020

Language: Английский

Citations

135
Circadian Photoentrainment in Mice and Humans DOI Creative Commons
F. Foster, Steven Hughes, Stuart N. Peirson

et al.

Biology, Journal Year: 2020, Volume and Issue: 9(7), P. 180 - 180

Published: July 21, 2020

Light around twilight provides the primary entrainment signal for circadian rhythms. Here we review mechanisms and responses of mouse human systems to light. Both utilize a network photosensitive retinal ganglion cells (pRGCs) expressing photopigment melanopsin (OPN4). In both species action spectra functional expression OPN4 in vitro show that has λmax close 480 nm. Anatomical findings demonstrate there are multiple pRGC sub-types, with some evidence mice, but little humans, regarding their roles regulating physiology behavior. Studies non-human primates rods cones project can modulate light pRGCs. Such an integration signals enables detect dim light, higher intensities intermittent exposure, whilst measures bright over extended periods time. Although photoreceptor similar, sensitivity thresholds differ markedly between mice humans. Mice entrain at approximately 1 lux few minutes, humans require high irradiance (>100's lux) long duration (>30 min). The basis this difference remains unclear. As our exposure is highly dynamic, because interactions complex difficult model, attempts develop evidence-based lighting enhance very challenging. A way forward will be define artificial natural "real world" where intensity, duration, spectral quality, time day, history age each assessed.

Language: Английский

Citations

124
Functional diversity of human intrinsically photosensitive retinal ganglion cells DOI Open Access
Ludovic S. Mure, Frans Vinberg, Anne Hanneken

et al.

Science, Journal Year: 2019, Volume and Issue: 366(6470), P. 1251 - 1255

Published: Dec. 6, 2019

Sensing light without forming images In the rodent retina, intrinsically photosensitive retinal ganglion cells (ipRGCs) entrain circadian rhythms, modulate mood, and signal pupillary accommodation. Such responses are light-driven but not image-based. Working with donated human organ tissues, Mure et al. used electrophysiological approaches to identify ipRGCs in retina. The retina has more cones than retinas of nocturnal mice rats. Differences sensitivity, latency, duration identified three subtypes ipRGCs. Science , this issue p. 1251

Language: Английский

Citations

112
Distinct ipRGC subpopulations mediate light’s acute and circadian effects on body temperature and sleep DOI Creative Commons
Alan C. Rupp, Michelle Ren,

Cara M. Altimus

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: July 23, 2019

The light environment greatly impacts human alertness, mood, and cognition by both acute regulation of physiology indirect alignment circadian rhythms. These processes require the melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs), but relevant downstream brain areas involved remain elusive. ipRGCs project widely in brain, including to central pacemaker, suprachiasmatic nucleus (SCN). Here we show that body temperature sleep responses exposure are absent after genetic ablation all except a subpopulation projects SCN. Furthermore, chemogenetic activation avoid SCN, these sufficient for changes temperature. Our results challenge idea SCN is major relay effects on non-image forming behaviors identify sensory initiate light's profound sleep.

Language: Английский

Citations

101
Cell-type-specific binocular vision guides predation in mice DOI Creative Commons

Keith P. Johnson,

Michael J. Fitzpatrick, Lei Zhao

et al.

Neuron, Journal Year: 2021, Volume and Issue: 109(9), P. 1527 - 1539.e4

Published: March 29, 2021

Language: Английский

Citations

97
The Lighting Environment, Its Metrology, and Non-visual Responses DOI Creative Commons
Luc J. M. Schlangen, Luke Price

Frontiers in Neurology, Journal Year: 2021, Volume and Issue: 12

Published: March 4, 2021

International standard CIE S 026:2018 provides lighting professionals and field researchers in chronobiology with a method to characterize light exposures respect non-visual photoreception responses. This defines five spectral sensitivity functions that describe optical radiation for its ability stimulate each of the α-opic retinal photoreceptor classes contribute effects humans via intrinsically-photosensitive ganglion cells (ipRGCs). The also recently published an open-access toolbox calculates all quantities ratios metrology photometric, radiometric photon systems, based on either measured (user-defined) spectrum or selected illuminants (A, D65, E, FL11, LED-B3) built into toolbox. For wide variety ecologically-valid conditions, melanopsin-based ipRGCs has been shown account responses, from shifting timing nocturnal sleep melatonin secretion regulating steady-state pupil diameter. Recent findings continue confirm photopigment melanopsin plays role visual may have significant influence brightness perception aspects spatial vision. Although knowledge concerning extent which rods cones interact driving is still growing, position statement used melanopic equivalent daylight (D65) illuminance preliminary guidance applying “proper at proper time” manipulate Further this approach awaited participants 2nd Workshop Circadian Neurophysiological Photometry (in Manchester, August 2019). new 026 enables traceable measurements formal, quantitative specification personal exposures, photic interventions designs. Here, we apply everyday sources including natural time series, range LED products and, using toobox, smartphone display screen. collection examples suggests ways variations content over day can be adopted strategies use support human health well-being.

Language: Английский

Citations

90
Feature Detection by Retinal Ganglion Cells DOI Creative Commons
Daniel Kerschensteiner

Annual Review of Vision Science, Journal Year: 2022, Volume and Issue: 8(1), P. 135 - 169

Published: April 6, 2022

Retinal circuits transform the pixel representation of photoreceptors into feature representations ganglion cells, whose axons transmit these to brain. Functional, morphological, and transcriptomic surveys have identified more than 40 retinal cell (RGC) types in mice. RGCs extract features varying complexity; some simply signal local differences brightness (i.e., luminance contrast), whereas others detect specific motion trajectories. To understand retina, we need know how give rise diverse RGC representations. A catalog set, turn, is fundamental understanding visual processing Anterograde tracing indicates that innervate 50 areas mouse Current maps connecting brain are rudimentary, as our signals transformed downstream guide behavior. In this article, I review selectivities RGCs, they arise, utilized downstream. Not only knowledge behavioral purpose critical for contributions vision; it can also us most relevant space.

Language: Английский

Citations

70