LncRNA LINC00942 promotes chemoresistance in gastric cancer by suppressing MSI2 degradation to enhance c‐Myc mRNA stability

Clinical and Translational Medicine, Journal Year: 2022, Volume and Issue: 12(1)

Published: Jan. 1, 2022

Language: Английский

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Non-coding RNA in cancer drug resistance: Underlying mechanisms and clinical applications

Frontiers in Oncology, Journal Year: 2022, Volume and Issue: 12

Published: Aug. 17, 2022

Cancer is one of the most frequently diagnosed malignant diseases worldwide, posing a serious, long-term threat to patients’ health and life. Systemic chemotherapy remains first-line therapeutic approach for recurrent or metastatic cancer patients after surgery, with potential effectively extend patient survival. However, development drug resistance seriously limits clinical efficiency ultimately results in treatment failure death. A large number studies have shown that non-coding RNAs (ncRNAs), particularly microRNAs, long RNAs, circular are widely involved regulation resistance. Their dysregulation contributes by modulating expression specific target genes cellular apoptosis, autophagy, efflux, epithelial-to-mesenchymal transition (EMT), stem cells (CSCs). Moreover, some ncRNAs also possess great as efficient, biomarkers diagnosis prognosis well targets patients. In this review, we summarize recent findings on emerging role underlying mechanisms focus their applications treatment. This information will be benefit early prognostic assessments ncRNA-based strategies

Language: Английский

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Writers, readers, and erasers RNA modifications and drug resistance in cancer

Molecular Cancer, Journal Year: 2024, Volume and Issue: 23(1)

Published: Aug. 30, 2024

Drug resistance in cancer cells significantly diminishes treatment efficacy, leading to recurrence and metastasis. A critical factor contributing this is the epigenetic alteration of gene expression via RNA modifications, such as N6-methyladenosine (m6A), N1-methyladenosine (m1A), 5-methylcytosine (m5C), 7-methylguanosine (m7G), pseudouridine (Ψ), adenosine-to-inosine (A-to-I) editing. These modifications are pivotal regulating splicing, translation, transport, degradation, stability. Governed by "writers," "readers," "erasers," impact numerous biological processes progression, including cell proliferation, stemness, autophagy, invasion, apoptosis. Aberrant can lead drug adverse outcomes various cancers. Thus, targeting modification regulators offers a promising strategy for overcoming enhancing efficacy. This review consolidates recent research on role prevalent resistance, with focus m6A, m1A, m5C, m7G, Ψ, A-to-I Additionally, it examines regulatory mechanisms linked underscores existing limitations field.

Language: Английский

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Progress in the studies on the molecular mechanisms associated with multidrug resistance in cancers

Acta Pharmaceutica Sinica B, Journal Year: 2022, Volume and Issue: 13(3), P. 982 - 997

Published: Oct. 7, 2022

Chemotherapy is one of the important methods to treat cancer, and emergence multidrug resistance (MDR) major cause for failure cancer chemotherapy. Almost all anti-tumor drugs develop drug over a period time application in patients, reducing their effects on killing cells. Chemoresistance can lead rapid recurrence cancers ultimately patient death. MDR may be induced by multiple mechanisms, which are associated with complex process genes, factors, pathways, steps, today MDR-associated mechanisms largely unknown. In this paper, from aspects protein–protein interactions, alternative splicing (AS) pre-mRNA, non-coding RNA (ncRNA) mediation, genome mutations, variance cell functions, influence tumor microenvironment, we summarize molecular cancers. end, prospects exploration antitumor that reverse briefly discussed angle systems improved targeting properties, biocompatibility, availability, other advantages.

Language: Английский

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MiR-124-3p impedes the metastasis of non-small cell lung cancer via extracellular exosome transport and intracellular PI3K/AKT signaling

Biomarker Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Jan. 4, 2023

Metastasis is a significant factor that affects the survival of patients with non-small cell lung cancer (NSCLC). Nevertheless, molecular regulatory mechanism underlying metastasis currently not fully understood. This study aims to identify important role miR-124-3p in NSCLC, thereby providing potential therapeutic intervention.Exosome secretion was determined by Nanoparticle Tracking Analysis (NTA) and uptake measured fluorescence inverted microscope. The binding between its upstream or downstream target genes validated experimentally Luciferase reporter. Cells migration evaluated transwell assays. Transcriptome sequencing on A549 carried out verify signaling pathway regulation. Western blotting analysis used assess level AKT, p-AKT, PI3K, p-PI3K protein expression NSCLC lines. suppress tumor verified xenograft model.Exosomes were more abundant serum from advanced (n = 24 patients) than these early-stage 30 patients), which suggested correlation amount exosome NSCLC. Interestingly, release, cells notably inhibited miR-124-3p. LINC00511 suppressed facilitate transport due as competitive endogenous RNA for could directly 3'-UTR Rab27a inhibit prevent invasion. Aside inhibition transport, activation PI3K/AKT intracellular environment. Finally, measuring subcutaneous weight volume metastasis, we also demonstrated growth vivo.In significantly through extracellular signaling. These findings provide new insights toward better understanding suggest treatment biomarker

Language: Английский

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RNA modification in cardiovascular disease: implications for therapeutic interventions

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: Oct. 27, 2023

Abstract Cardiovascular disease (CVD) is the leading cause of death in world, with a high incidence and youth-oriented tendency. RNA modification ubiquitous indispensable cell, maintaining cell homeostasis function by dynamically regulating gene expression. Accumulating evidence has revealed role aberrant expression CVD caused dysregulated modification. In this review, we focus on nine common modifications: N 6 -methyladenosine (m A), 1 5-methylcytosine 5 C), 7 -methylguanosine G), 4 -acetylcytosine (ac pseudouridine (Ψ), uridylation, adenosine-to-inosine (A-to-I) editing, modifications U34 tRNA wobble. We summarize key regulators their effects expression, such as splicing, maturation, transport, stability, translation. Then, based classification CVD, mechanisms which occurs progresses through are discussed. Potential therapeutic strategies, therapy, reviewed these mechanisms. Herein, some (such stroke peripheral vascular disease) not included due to limited availability literature. Finally, prospective applications challenges discussed for purpose facilitating clinical Moreover, look forward more studies exploring roles future, there substantial uncultivated areas be explored.

Language: Английский

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Cancer chemoresistance and its mechanisms: Associated molecular factors and its regulatory role

Medical Oncology, Journal Year: 2023, Volume and Issue: 40(9)

Published: Aug. 7, 2023

Language: Английский

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GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: Dec. 18, 2023

Abstract Background Research has indicated that long-term sleep deprivation can lead to immune dysfunction and participate in the occurance progression of tumors. However, relationship between colon cancer remains unclear. This study explored specific mechanism through which promotes proliferation migration cancer, with a focus on neurotransmitter GABA. Methods Chronic mice model were used investigate effect disorder We detected levels peripheral blood using ELISA. CCK-8 assay, colony formation wound healing transwell assay performed GABA cells, while immunofluorescence showed distribution macrophages lung metastatic tissues. isolated exosomes from GABA-induced culture medium explore effects cells macrophages. Gain- loss-of-function experiments, luciferase report analysis, immunohistochemistry, cytokine detection reveal crosstalk Results Sleep promote level cell migration. Immunofluorescence analysis revealed metastasis is associated enhanced recruitment lungs. The co-culture results intensified M2 polarization macrophage induced by cells. due activation MAPK pathway tumor-derived exosomal miR-223-3p. Furthermore, M2-like tumor secreting IL-17. also identified an endogenous miR-223-3p downregulation E3 ligase CBLB, enhances stability cMYC protein augments ability. Notably, acts as transcription factor regulate expression Conclusion Our suggest GABA, leading CBLB inhibition ubiquitination. Simultaneously, extracellular polarization, leads secretion IL-17, further enhancing

Language: Английский

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The prevention of multi-drug resistance in cancers through the application of nanotechnology-based targeted delivery systems for combination therapies involving traditional Chinese medicine

Pharmacological Research - Modern Chinese Medicine, Journal Year: 2024, Volume and Issue: 10, P. 100386 - 100386

Published: Feb. 4, 2024

The most significant obstacle in addressing cancer is the development of multidrug resistance during chemotherapy. Cancer cells have ability to endure numerous cycles traditional chemotherapy drugs before ultimately developing treatment. Combining a strategy used address problem resistance. Persistent challenges with synthetic include catastrophic adverse reactions and other consequences. Natural compounds originating from Chinese medicine are acknowledged for their minimal toxicity possible benefits reversing induced by mainstream chemotherapeutic or targeted treatments. (MDR) an immediate clinical justification unsatisfactory recurrence metastasis precipitated MDR, leading high risk death. unique nano-delivery method therapeutic management MDR cancers has emerged as key use nanotechnology. Because its anticancer prospective, (TCM)-combination therapy emerging treatment modality essential option cancer. By precisely regulating release medicines delivering pharmaceuticals cancerous cells, nano drug-delivery devices offer amazing pave way growth TCM-combination therapies. present review examines literature on three important topics: mechanism chemotherapy, role herbs formulation treatment, incorporation TCM nanotechnology-based drug delivery systems circumvent limitations system. Major sources work PubMed, Google Scholar, ResearchGate, Embase, ScienceDirect. All aspects pertaining TCM, nanotechnology more than 50 years were considered, emphasis formulation, various forms advances system result nanotechnology, everything else between. As becomes closely associated realm practice broadening. Furthermore, it enhances prospects future research facilitates advancement modern Traditional Medicine (TCM). integrating into meticulously designed system, will enhance both treat prevent diseases even greater efficacy previously. This article nano-TCM explores characteristics, compositions, relevant techniques preparation application MDR. In addition, comprehensive elucidation had been given respect objective this study was analyze existing body (TCM) that can be encompasses like active components utilization (MDR), potential advancements obstacles TCM-based combinations.

Language: Английский

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LINC00942 inhibits ferroptosis and induces the immunosuppression of regulatory T cells by recruiting IGF2BP3/SLC7A11 in hepatocellular carcinoma

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(1)

Published: Feb. 1, 2024

Abstract Hepatocellular carcinoma (HCC) is a common malignant tumor with high recurrence rate and poor prognosis. Long intergenic nonprotein coding RNA 942 (LINC00942) reported to be related ferroptosis the immune response in HCC serves as an oncogene various cancers. This research aimed explore contribution of LINC00942 progression. Functional assays were used evaluate functional role vitro vivo. Mechanistic conducted assess association insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3) solute carrier family 7 member 11 (SLC7A11) regulatory pattern cells. was found exhibit upregulation tissue facilitated cell proliferation, suppressed ferroptosis, converted naive CD4 + T cells inducible Treg (iTreg) by regulating SLC7A11. Furthermore, SLC7A11 expression positively modulated IGF2BP3 shared RNA-binding (RBP) for The between 3′ untranslated region verified, recruit promote stability m6A-dependent manner. Moreover, mouse proliferation inhibited, number FOXP3 CD25 increased, while enhanced after knockdown suppresses induces immunosuppression recruiting enhance stability, which may provide novel therapeutic targets HCC.

Language: Английский

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