Analytical Techniques for Metabolomic Studies: A Review

Bioanalysis, Journal Year: 2019, Volume and Issue: 11(24), P. 2297 - 2318

Published: Dec. 1, 2019

Metabolomics is the comprehensive study of small-molecule metabolites. Obtaining a wide coverage metabolome challenging because broad range physicochemical properties small molecules. To compounds interest spectroscopic (NMR), spectrometric (MS) and separation techniques (LC, GC, supercritical fluid chromatography, CE) are used. The choice for given technique influenced by sample matrix, concentration metabolites, amount sample. This review discusses most commonly used analytical metabolomic studies, including their advantages, drawbacks some applications.

Language: Английский

---

Novel Strategies to Address the Challenges in Top-Down Proteomics

Journal of the American Society for Mass Spectrometry, Journal Year: 2021, Volume and Issue: 32(6), P. 1278 - 1294

Published: May 13, 2021

Top-down mass spectrometry (MS)-based proteomics is a powerful technology for comprehensively characterizing proteoforms to decipher post-translational modifications (PTMs) together with genetic variations and alternative splicing isoforms toward proteome-wide understanding of protein functions. In the past decade, top-down has experienced rapid growth benefiting from groundbreaking technological advances, which have begun reveal potential basic biological functions, unraveling disease mechanisms, discovering new biomarkers. However, many challenges remain be addressed. this Account & Perspective, we discuss major currently facing field, particularly in solubility, proteome dynamic range, complexity, data analysis, proteoform-function relationship, analytical throughput precision medicine. We specifically review developments addressing these an emphasis on our research group's efforts, including development MS-compatible surfactants solubilization, functionalized nanoparticles enrichment low-abundance proteoforms, strategies multidimensional chromatography separation proteins, comprehensive user-friendly software package proteomics. also made efforts connect functions provide visions what future holds

Language: Английский

---

To metabolomics and beyond: a technological portfolio to investigate cancer metabolism

Signal Transduction and Targeted Therapy, Journal Year: 2023, Volume and Issue: 8(1)

Published: March 22, 2023

Abstract Tumour cells have exquisite flexibility in reprogramming their metabolism order to support tumour initiation, progression, metastasis and resistance therapies. These reprogrammed activities include a complete rewiring of the bioenergetic, biosynthetic redox status sustain increased energetic demand cells. Over last decades, cancer field has seen an explosion new biochemical technologies giving more tools than ever before navigate this complexity. Within cell or tissue, metabolites constitute direct signature molecular phenotype thus profiling concrete clinical applications oncology. Metabolomics fluxomics, are key technological approaches that mainly revolutionized enabling researchers both qualitative mechanistic model cancer. Furthermore, upgrade from bulk single-cell analysis provided unprecedented opportunity investigate biology at cellular resolution allowing depth quantitative complex heterogenous diseases. More recently, advent functional genomic screening allowed identification pathways, processes, biomarkers novel therapeutic targets concert with other allow patient stratification treatment regimens. This review is intended be guide for metabolism, highlighting current emerging technologies, emphasizing advantages, disadvantages potential leading development innovative anti-cancer

Language: Английский

---

Exploring the Potential of Bioactive Peptides: From Natural Sources to Therapeutics

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1391 - 1391

Published: Jan. 23, 2024

Bioactive peptides, specific protein fragments with positive health effects, are gaining traction in drug development for advantages like enhanced penetration, low toxicity, and rapid clearance. This comprehensive review navigates the intricate landscape of peptide science, covering discovery to functional characterization. Beginning a peptidomic exploration natural sources, emphasizes search novel peptides. Extraction approaches, including enzymatic hydrolysis, microbial fermentation, specialized methods disulfide-linked extensively covered. Mass spectrometric analysis techniques data acquisition identification, such as liquid chromatography, capillary electrophoresis, untargeted analysis, bioinformatics, thoroughly outlined. The bioactivity incorporates various methodologies, from vitro assays silico techniques, advanced approaches phage display cell-based assays. also discusses structure–activity relationship context antimicrobial peptides (AMPs), ACE-inhibitory (ACEs), antioxidative (AOPs). Concluding key findings future research directions, this interdisciplinary serves reference, offering holistic understanding their potential therapeutic applications.

Language: Английский

---

Mass spectrometry using electrospray ionization

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: March 23, 2023

Language: Английский

---

Top-down proteomics

Nature Reviews Methods Primers, Journal Year: 2024, Volume and Issue: 4(1)

Published: June 13, 2024

Language: Английский

---

Peptidomics and proteomics based on CE‐MS as a robust tool in clinical application: The past, the present, and the future

Electrophoresis, Journal Year: 2019, Volume and Issue: 40(18-19), P. 2294 - 2308

Published: May 6, 2019

Capillary electrophoresis combined with mass spectrometry (CE-MS) has been used for several years the investigation of proteins and peptides as biomarkers diagnosis prognosis diseases. In addition, technology recently introduced to support stratification patients in clinical trials large studies. this review, we aim at presenting development CE-MS over last 20 years, by focusing on potential proteome peptidome analysis highlighting some key technical issues advancements that have made context towards implementation. Based reviewed literature, it become evident is now an accepted tool application disease areas. Apart from a critical overview current state-of-the-art CE-MS, also indicate expected developments future use.

Language: Английский

---

Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research

Mass Spectrometry Reviews, Journal Year: 2019, Volume and Issue: 38(6), P. 445 - 460

Published: Aug. 12, 2019

Progress in proteomics research has led to a demand for powerful analytical tools with high separation efficiency and sensitivity confident identification quantification of proteins, posttranslational modifications, protein complexes expressed cells tissues. This significantly increased interest capillary electrophoresis‐mass spectrometry (CE‐MS) the past few years. review provides highlights recent advances CE‐MS research, including short introduction top‐down mass native (native MS), as well detailed overview CE methods. Both potential limitations these methods analysis proteins peptides synthetic biological samples challenges are discussed, along perspectives about future direction CE‐MS. @ 2019 Wiley Periodicals, Inc. Mass Spec Rev 00:1–16, 2019.

Language: Английский

---

MEATabolomics: Muscle and Meat Metabolomics in Domestic Animals

Metabolites, Journal Year: 2020, Volume and Issue: 10(5), P. 188 - 188

Published: May 11, 2020

In the past decades, metabolomics has been used to comprehensively understand a variety of food materials for improvement and assessment quality. Farm animal skeletal muscles meat are one major targets characterization exploration biomarkers in production system. For identification potential control quality, studies with (MEATabolomics) conducted combination analyses quality traits, focusing on specific factors associated genetic background sensory scores, or conditions feeding system treatments processes such as postmortem storage, processing, hygiene control. Currently, most MEATabolomics approaches combine separation techniques (gas liquid chromatography, capillary electrophoresis)–mass spectrometry (MS) nuclear magnetic resonance (NMR) downstream multivariate analyses, depending polarity and/or hydrophobicity targeted metabolites. Studies employing these provide useful information monitor traits efficiently animals behind is expected improve knowledge methodologies breeding feeding, storage prediction

Language: Английский

---

Mass Spectrometry of Nucleic Acid Noncovalent Complexes

Chemical Reviews, Journal Year: 2021, Volume and Issue: 122(8), P. 7720 - 7839

Published: Sept. 30, 2021

Nucleic acids have been among the first targets for antitumor drugs and antibiotics. With unveiling of new biological roles in regulation gene expression, specific DNA RNA structures become very attractive targets, especially when corresponding proteins are undruggable. Biophysical assays to assess target structure as well ligand binding stoichiometry, affinity, specificity, modes part drug development process. Mass spectrometry offers unique advantages a biophysical method owing its ability distinguish each stoichiometry present mixture. In addition, advanced mass approaches (reactive probing, fragmentation techniques, ion mobility spectrometry, spectroscopy) provide more detailed information on complexes. Here, we review fundamentals all particularities studying noncovalent nucleic acid structures, then what has learned thanks self-assemblies (e.g., duplexes or G-quadruplexes), their complexes with ligands.

Language: Английский

---