Exploring the Potential of Bioactive Peptides: From Natural Sources to Therapeutics

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1391 - 1391

Published: Jan. 23, 2024

Bioactive peptides, specific protein fragments with positive health effects, are gaining traction in drug development for advantages like enhanced penetration, low toxicity, and rapid clearance. This comprehensive review navigates the intricate landscape of peptide science, covering discovery to functional characterization. Beginning a peptidomic exploration natural sources, emphasizes search novel peptides. Extraction approaches, including enzymatic hydrolysis, microbial fermentation, specialized methods disulfide-linked extensively covered. Mass spectrometric analysis techniques data acquisition identification, such as liquid chromatography, capillary electrophoresis, untargeted analysis, bioinformatics, thoroughly outlined. The bioactivity incorporates various methodologies, from vitro assays silico techniques, advanced approaches phage display cell-based assays. also discusses structure–activity relationship context antimicrobial peptides (AMPs), ACE-inhibitory (ACEs), antioxidative (AOPs). Concluding key findings future research directions, this interdisciplinary serves reference, offering holistic understanding their potential therapeutic applications.

Language: Английский

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Analytical techniques for multiplex analysis of protein biomarkers

Expert Review of Proteomics, Journal Year: 2020, Volume and Issue: 17(4), P. 257 - 273

Published: April 2, 2020

Introduction The importance of biomarkers for pharmaceutical drug development and clinical diagnostics is more significant than ever in the current shift toward personalized medicine. Biomarkers have taken a central position either as companion markers to support patient selection, or indicators aiming detect earliest perturbations indicative disease, minimizing therapeutic intervention even enabling disease reversal. Protein are particular interest given their role biochemical pathways. Hence, capabilities analyze multiple protein one assay highly interesting biomedical research.Areas covered We here review methods that suitable robust, high throughput, standardized, affordable analysis multiplex format. describe innovative developments immunoassays, vanguard laboratories, mass spectrometry, increasingly implemented biomarker analysis. Moreover, emerging techniques discussed with potentially improved capture, separation, detection will further boost analyses.Expert commentary clinically applied assays essential multi-protein signatures provide comprehensive information about biological systems single biomarkers, leading insights mechanisms diagnostics, effect

Language: Английский

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Peptidomics: A Review of Clinical Applications and Methodologies

Journal of Proteome Research, Journal Year: 2021, Volume and Issue: 20(8), P. 3782 - 3797

Published: July 16, 2021

Improvements in both liquid chromatography (LC) and mass spectrometry (MS) instrumentation have greatly enhanced proteomic small molecule metabolomic analysis recent years. Less focus has been on the improved capability to detect quantify bioactive peptides, even though exact sequences of peptide species produced can important biological consequences. Endogenous hormones, for example, are generated by targeted regulated cleavage peptides from their prohormone sequence. This process may include organ specific variants, as proglucagon is converted glucagon pancreas but glucagon-like peptide-1 (GLP-1) intestine, with raising, whereas GLP-1, an incretin, lowering blood glucose. Therefore, peptidomics workflows must preserve structure processed products prevent misidentification ambiguous species. The poor vivo vitro stability matrices a major factor that needs be considered when developing methods study them. bioinformatic data sets requires inclusion post-translational modifications, which critical function many peptides. review aims discuss contrast various extraction, analytical, bioinformatics approaches used human studies multitude matrices.

Language: Английский

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Clinical applications of plasma proteomics and peptidomics: Towards precision medicine

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2022, Volume and Issue: 16(6)

Published: May 1, 2022

In the context of precision medicine, disease treatment requires individualized strategies based on underlying molecular characteristics to overcome therapeutic challenges posed by heterogeneity. For this purpose, it is essential develop new biomarkers diagnose, stratify, or possibly prevent diseases. Plasma an available source that greatly reflects physiological and pathological conditions body. An increasing number studies are focusing proteins peptides, including many involving Human Proteome Project (HPP) Organization (HUPO), proteomics peptidomics techniques emerging as critical tools for developing novel medicine preventative measures. Excitingly, plasma toolbox exhibits a huge potential studying pathogenesis diseases (e.g., COVID-19 cancer), identifying valuable improving clinical management. However, enormous complexity wide dynamic range makes proteome profiling challenging. Herein, we summarize recent advances in with focus their roles cancer research, aiming emphasize significance applications medicine.

Language: Английский

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Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography (2019–2022)

Journal of Pharmaceutical Analysis, Journal Year: 2023, Volume and Issue: 13(4), P. 323 - 339

Published: Feb. 6, 2023

Capillary electrochromatography (CEC) plays a significant role in chiral separation via the double principle, partition coefficient difference between two phases, and electroosmotic flow-driven separation. Given distinct properties of inner wall stationary phase (SP), ability each SP differs from one another. Particularly, it provides large room for promising applications open tubular capillary (OT-CEC). We divided OT-CEC SPs developed over past four years into six types: ionic liquids, nanoparticle materials, microporous biomaterials, non-nanopolymers, others, to mainly introduce their characteristics drug There also added few classic that occurred within ten as supplements enrich features SP. Additionally, we discuss metabolomics, food, cosmetics, environment, biology analytes addition drugs. an increasingly may promote development electrophoresis (CE) combined with other instruments recent years, such CE mass spectrometry (CE/MS) ultraviolet light detector (CE/UV).

Language: Английский

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Capillary electrophoresis‐mass spectrometry for intact protein analysis: Pharmaceutical and biomedical applications (2018–March 2023)

Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(15)

Published: May 26, 2023

Capillary electrophoresis is recognized as a valued separation technique for its high efficiency, low sample consumption, good economic and ecological aspects, reproducibility, complementarity to traditional liquid chromatography techniques. experiments are generally performed utilizing optical detection, such ultraviolet or fluorescence detectors. However, in order provide structural information, capillary hyphenated highly sensitive selective mass spectrometry has been developed overcome the limitations of detections. electrophoresis‐mass increasingly popular protein analysis, including biopharmaceutical biomedical research. It frequently applied determination physicochemical biochemical parameters proteins, offers excellent performance in‐depth characterizations biopharmaceuticals at various levels also already proven promising tool biomarker discovery. In this review, we focus on possibilities analysis their intact level. Various modes interfaces, well approaches prevent adsorption enhance loading capacity, discussed recent (2018–March 2023) developments applications field summarized.

Language: Английский

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Urine proteomics for prediction of disease progression in patients with IgA nephropathy

Nephrology Dialysis Transplantation, Journal Year: 2020, Volume and Issue: 37(1), P. 42 - 52

Published: Nov. 11, 2020

Abstract Background Risk of kidney function decline in immunoglobulin A (IgA) nephropathy (IgAN) is significant and may not be predicted by available clinical histological tools. To serve this unmet need, we aimed at developing a urinary biomarker-based algorithm that predicts rapid disease progression IgAN, thus enabling personalized risk stratification. Methods In multicentre study, urine samples were collected 209 patients with biopsy-proven IgAN. Progression was defined tertiles the annual change estimated glomerular filtration rate (eGFR) during follow-up. Urine analysed using capillary electrophoresis coupled mass spectrometry. The area under receiver operating characteristic curve (AUC) used to evaluate prediction models. Results Of patients, 64% male. Mean age 42 years, mean eGFR 63 mL/min/1.73 m2 median proteinuria 1.2 g/day. We identified 237 peptides showing difference abundance according tertile change. These included fragments apolipoprotein C-III, alpha-1 antitrypsin, different collagens, fibrinogen alpha beta, titin, haemoglobin subunits, sodium/potassium-transporting ATPase subunit gamma, uromodulin, mucin-2, fractalkine, polymeric Ig receptor insulin. An based on these protein (IgAN237) showed added value for IgAN [AUC 0.89; 95% confidence interval (CI) 0.83–0.95], as compared parameters (age, gender, proteinuria, arterial pressure) alone (0.72; CI 0.64–0.81). Conclusions peptide classifier progressive loss significantly better than alone.

Language: Английский

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Urinary peptides in heart failure: a link to molecular pathophysiology

European Journal of Heart Failure, Journal Year: 2021, Volume and Issue: 23(11), P. 1875 - 1887

Published: April 22, 2021

Abstract Aims Heart failure (HF) is a major public health concern worldwide. The diversity of HF makes it challenging to decipher the underlying complex pathological processes using single biomarkers. We examined association between urinary peptides and with reduced (HFrEF), mid‐range (HFmrEF) preserved (HFpEF) ejection fraction, defined based on European Society Cardiology guidelines, links these peptide biomarkers molecular pathophysiology. Methods results Analysable data from 5608 participants were available in Human Urinary Proteome database. profiles diagnosed HFrEF, HFmrEF, HFpEF controls matched for sex, age, estimated glomerular filtration rate, systolic diastolic blood pressure, diabetes hypertension compared applying Mann–Whitney test, followed by correction multiple testing. Unsupervised learning algorithms applied investigate groups similar profiles. A total 577 significantly associated sequenced, 447 which (77%) collagen fragments. In silico analysis suggested that biomarker abnormalities principally reflect changes turnover immune response, both fibrosis. clustering separated study into two clusters, 83% non‐HF allocated cluster 1, while 65% patients 2 ( P < 0.0001). No separation subtype was detectable. Conclusions failure, irrespective fraction subtype, differences abundance reflecting inflammation. These should be studied as tools early detection, prognostication, prediction therapeutic response.

Language: Английский

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Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Electrophoresis, Journal Year: 2021, Volume and Issue: 43(1-2), P. 82 - 108

Published: Oct. 11, 2021

Abstract The review provides a comprehensive overview of developments and applications high performance capillary microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrokinetic chromatography, electrochromatography) for analysis, microscale isolation, physicochemical characterization peptides from 2019 up to approximately the middle 2021. Advances in investigation electromigration properties methodology their such as sample preparation, sorption suppression, EOF control, detection, are presented. New individual CE CEC demonstrated several types shown. They include qualitative quantitative determination complex biomatrices, monitoring chemical enzymatic reactions changes, amino acid, sequence, chiral analyses, peptide mapping proteins. In addition, micropreparative separations significant parameters by described.

Language: Английский

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A urinary peptidomic profile predicts outcome in SARS-CoV-2-infected patients

EClinicalMedicine, Journal Year: 2021, Volume and Issue: 36, P. 100883 - 100883

Published: May 3, 2021

Language: Английский

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