International Immunopharmacology, Journal Year: 2025, Volume and Issue: 152, P. 114452 - 114452
Published: March 16, 2025
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1391 - 1391
Published: Jan. 23, 2024
Bioactive peptides, specific protein fragments with positive health effects, are gaining traction in drug development for advantages like enhanced penetration, low toxicity, and rapid clearance. This comprehensive review navigates the intricate landscape of peptide science, covering discovery to functional characterization. Beginning a peptidomic exploration natural sources, emphasizes search novel peptides. Extraction approaches, including enzymatic hydrolysis, microbial fermentation, specialized methods disulfide-linked extensively covered. Mass spectrometric analysis techniques data acquisition identification, such as liquid chromatography, capillary electrophoresis, untargeted analysis, bioinformatics, thoroughly outlined. The bioactivity incorporates various methodologies, from vitro assays silico techniques, advanced approaches phage display cell-based assays. also discusses structure–activity relationship context antimicrobial peptides (AMPs), ACE-inhibitory (ACEs), antioxidative (AOPs). Concluding key findings future research directions, this interdisciplinary serves reference, offering holistic understanding their potential therapeutic applications.
Language: Английский
Citations
46
Liver International, Journal Year: 2025, Volume and Issue: 45(2)
Published: Jan. 17, 2025
ABSTRACT Background Metabolic Dysfunction‐Associated Steatotic Liver Disease (MASLD) encompasses a spectrum of histological conditions ranging from simple steatosis to fibrosing steatohepatitis, and is risk factor for cardiovascular diseases (CVD). While oxidised apolipoproteins A B have been linked obesity CVD, the association between other MASLD yet be established. To fill this gap, we characterised circulating serum peptidome patients with MASLD. Methods We studied 87 biopsy‐confirmed 20 age‐ sex‐matched control (CTRL) subjects. first employed an untargeted LC‐MS/MS peptidomics approach (9 CTRL, 32 MASLD) identify key hits differentially modulated, subsequently validated most relevant findings through targeted in enlarged study population (87 CTRL). Results Untargeted identified several apolipoprotein peptide fragments, including ApoE ApoC‐III, significantly upregulated compared CTRL. Specifically focusing on oxidative status intact its major glycoforms (ApoC‐III 0 , ApoC‐III i ii ), observed marked reduction non‐oxidised forms these peptides alongside substantially increased levels their proteoforms versus controls (but not within disease stages). Oxidised fragments were also correlated obesity, insulin resistance, dyslipidaemia transaminases, suggesting potential link oxidation systemic/hepatic metabolic dysfunction. Conclusion Our data reveals previously unreported profile associated The functional clinical implications warrant further mechanistic investigation.
Language: Английский
Citations
1
Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 259 - 299
Published: Jan. 1, 2025
Language: Английский
Citations
1
PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2022, Volume and Issue: 16(6)
Published: May 1, 2022
In the context of precision medicine, disease treatment requires individualized strategies based on underlying molecular characteristics to overcome therapeutic challenges posed by heterogeneity. For this purpose, it is essential develop new biomarkers diagnose, stratify, or possibly prevent diseases. Plasma an available source that greatly reflects physiological and pathological conditions body. An increasing number studies are focusing proteins peptides, including many involving Human Proteome Project (HPP) Organization (HUPO), proteomics peptidomics techniques emerging as critical tools for developing novel medicine preventative measures. Excitingly, plasma toolbox exhibits a huge potential studying pathogenesis diseases (e.g., COVID-19 cancer), identifying valuable improving clinical management. However, enormous complexity wide dynamic range makes proteome profiling challenging. Herein, we summarize recent advances in with focus their roles cancer research, aiming emphasize significance applications medicine.
Language: Английский
Citations
37
Journal of Separation Science, Journal Year: 2023, Volume and Issue: 46(12)
Published: Feb. 26, 2023
This review gives a wide overview of recent advances and applications capillary electrophoresis microchip methods in the fields proteomics peptidomics period from mid‐2018 up to end 2022. The methodological topics covering sample preparation concentration techniques, hyphenation with mass spectrometry, multidimensional separations by on‐line or off‐line coupled different liquid chromatography techniques are described new developments both bottom‐up top‐down approaches presented. In addition, various proteomic peptidomic studies demonstrated. They include monitoring protein posttranslational modifications biological biochemical research, clinical proteomics, food analysis.
Language: Английский
Citations
22
Electrophoresis, Journal Year: 2023, Volume and Issue: 45(1-2), P. 165 - 198
Published: Sept. 5, 2023
Abstract This review article brings a comprehensive survey of developments and applications high‐performance capillary microchip electromigration methods (zone electrophoresis in free solution or sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, electrochromatography) for analysis, micropreparation, physicochemical characterization peptides the period from 2021 up to ca. middle 2023. Progress study properties various aspects their such as sample preparation, adsorption suppression, electroosmotic flow regulation, detection, are presented. New particular demonstrated, several types reported. They cover qualitative quantitative analysis synthetic isolated determination complex biomatrices, peptide profiling biofluids tissues, monitoring chemical enzymatic reactions changes peptides. include also amino acid sequence peptides, mapping proteins, separation stereoisomers chiral analyses. In addition, micropreparative separations interactions with other (bio)molecules by above CE described.
Language: Английский
Citations
18
BME Frontiers, Journal Year: 2023, Volume and Issue: 4
Published: Jan. 1, 2023
Extensive effort has been devoted to the discovery, development, and validation of biomarkers for early disease diagnosis prognosis as well rapid evaluation response therapeutic interventions. Genomic transcriptomic profiling are well-established means identify disease-associated biomarkers. However, analysis peptidomes can also novel peptide or signatures that provide sensitive specific diagnostic prognostic information malignant, chronic, infectious diseases. Growing evidence suggests peptidomic changes in liquid biopsies may more effectively detect pathophysiology than other molecular methods. Knowledge gained from peptide-based diagnostic, therapeutic, imaging approaches led promising new theranostic applications increase their bioavailability target tissues at reduced doses decrease side effects improve treatment responses. despite major advances, multiple factors still affect utility data. This review summarizes several remaining challenges biomarker discovery use diagnostics, with a focus on technological advances detection, identification, monitoring personalized medicine.
Language: Английский
Citations
17
The Analyst, Journal Year: 2021, Volume and Issue: 147(1), P. 40 - 47
Published: Nov. 16, 2021
Metal-organic frameworks (MOFs) have attracted extensive attention for the development of colorimetric detection methods due to their ease modification and high density active sites. However, most reported sensors based on MOFs show only a single nanozyme activity. Herein, bifunctional enzyme activities hexagonal prism Cu MOF with fumaric acid as ligand (Cu FMA), namely laccase-like activity under alkaline conditions (pH = 8) peroxidase-like acidic 4), were verified. The specificity FMA at different pH values may be presence Cu+ center introduced by weak reducibility FMA. At 8, centers are beneficial dissociating H-O bonds phenolic compounds laccase system. In contrast, dissociation is weakened 4, which prompts breaking O-O H2O2 Fenton-like reaction peroxidase Based dual activities, exhibit outstanding detectability epinephrine glucose linear ranges 2.7-54.6 μM 0.01-0.8 mM limits 1.1 2.28 × 10-7 M, respectively. sensor can applied detecting measuring in human serum samples. This work paves way used basis rational regulation nanozymes multifunctional applications completely independent conditions.
Language: Английский
Citations
38
PROTEOMICS, Journal Year: 2021, Volume and Issue: 21(23-24)
Published: Sept. 30, 2021
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is now the main analytical method for identification and quantification of peptides proteins in biological samples. In modern research, biomarkers their quantitative comparison between samples are becoming increasingly important discovery, validation, monitoring. Such data can be obtained following specific signals after fragmentation using multiple reaction monitoring (MRM) parallel (PRM) methods, with high specificity, accuracy, reproducibility. addition, these methods allow measurement amount post-translationally modified forms isoforms proteins. This review article describes basic principles MRM assays, guidelines sample preparation, recent advanced MRM-based strategies, applications illustrative perspectives MRM/PRM clinical research molecular biology.
Language: Английский
Citations
36
Journal of Inflammation Research, Journal Year: 2022, Volume and Issue: Volume 15, P. 1457 - 1470
Published: Feb. 1, 2022
GLP-1 based therapy represents a new treatment option for inflammatory bowel disease. Ban-Lan-Gen (BLG) granule, known anti-viral TCM formulation, exhibits potential anti-inflammatory activities in treating various kinds of inflammation. However, its effect on colitis and the underlying mechanisms remain unknown.Dextran sulfate sodium (DSS)-induced chronic relapsing mice was established. The disease activity index, histological sign damage, levels proinflammatory cytokines were performed to assess protective effects BLG. Serum level colonic Gcg, GPR41 GRP43 expression, community compositions gut microbiota, SCFAs feces release from primary murine colon epithelial cells characterize BLG microbiota SCFA derived-GLP-1 production.BLG significantly alleviated body weight loss, DAI, shortening, tissue pro-inflammatory cytokine TNF-α, IL-1β IL-6 tissues. Moreover, could observably restore expression serum colitic mice, as well correct alteration by increasing abundances SCFA-producing bacteria, eg, Akkermansia Prevotellaceae_UCG-001, decreasing Eubacterium_xylanophilum_group, Ruminococcaceae_UCG-014, Intestinimonas, Oscillibacter. Furthermore, markedly increase mice. In parallel, ex vivo assay also showed that extract BLG-treatment greatly stimulate secretion cells.These findings suggest anti-colitis are achieved at least partly regulating restoring production, has be developed promising agent colitis.
Language: Английский
Citations
28