Review: Recent progress in fluorescent molecular systems for the detection of disease-related biomarkers in biofluids

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 494, P. 215336 - 215336

Published: July 16, 2023

Language: Английский

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The role of the oral cavity in SARS-CoV-2- and other viral infections

Clinical Oral Investigations, Journal Year: 2023, Volume and Issue: 27(S1), P. 15 - 22

Published: June 13, 2023

Abstract Objective This study aims to review the role of oral cavity in SARS-CoV-2- and other viral upper respiratory tract infections. Material methods Data reviewed text have been researched online also reflect personal expertise. Results Numerous viruses replicate are transmitted via aerosols (< 5 µm) droplets (> µm). SARS-CoV-2 replication has documented airways as well mucosa salivary glands. These sites virus reservoirs that can infect organs, e.g., lungs gastrointestinal tract, individuals. Laboratory diagnosis focuses on real-time PCR; antigen tests less sensitive. For screening monitoring infections, nasopharyngeal swabs tested; saliva is a good more comfortable alternative. Physical means like social distancing or masks proven successful reduce risk infection. Both wet-lab clinical studies confirm mouth rinses effective against viruses. Antiviral inactivate all cavity. Conclusions The plays an important infections tract: it serves portal entry, site replication, source infection by aerosols. but antiviral help spread contribute control.

Language: Английский

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Advances in human papillomavirus detection and molecular understanding in head and neck cancers: Implications for clinical management

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(6)

Published: June 1, 2024

Abstract Head and neck cancers (HNCs), primarily head squamous cell carcinoma (HNSCC), are associated with high‐risk human papillomavirus (HR HPV), notably HPV16 HPV18. HPV status guides treatment predicts outcomes, distinct molecular pathways in HPV‐driven HNSCC influencing survival rates. HNC incidence is rising globally, regional variations reflecting diverse risk factors, including tobacco, alcohol, infection. Oropharyngeal attributed to have significantly increased, particularly regions like the United States. The genome, characterized by oncoproteins E6 E7, disrupts crucial cycle regulators, tumor protein p53 (TP53) retinoblastoma (Rb), contributing pathogenesis. P16 immunohistochemistry (IHC) a reliable surrogate marker for positivity, while situ hybridization polymerase chain reaction (PCR) techniques, reverse transcription‐quantitative PCR (RT‐qPCR), offer sensitive detection. Liquid‐based RT‐qPCR, especially saliva, shows promise noninvasive detection, offering simplicity, cost‐effectiveness, patient compliance. These advancements enhance diagnostic accuracy, guide decisions, improve outcomes management. In conclusion, advances detection understanding significant clinical management implications. Integrating these into routine practice could ultimately outcomes.

Language: Английский

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Diagnostic Accuracy of Liquid Biomarkers in Airway Diseases: Toward Point-of-Care Applications

Frontiers in Medicine, Journal Year: 2022, Volume and Issue: 9

Published: June 6, 2022

Background Liquid biomarkers have shown increasing utility in the clinical management of airway diseases. Salivary and blood samples are particularly amenable to point-of-care (POC) testing due simple specimen collection processing. However, very few POC tests successfully progressed application uncertainty unpredictability surrounding their diagnostic accuracy. Objective To review liquid diseases with well-established accuracies discuss prospects for future applications. Methodology A literature publications indexed Medline or Embase was performed evaluate accuracy chronic obstructive pulmonary disease (COPD), asthma, laryngopharyngeal reflux (LPR), COVID-19. Results Of 3,628 studies, 71 fulfilled inclusion criteria. Sputum eosinophils were most frequently investigated asthma COPD. pepsin only biomarker a well-documented diagnosis LPR. Inflammatory (e.g., CRP, D-dimers, ferritin) found be useful predict severity, complications, mortality related COVID-19 infection. Conclusion Multiple thus settings.

Language: Английский

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New Frontiers in Autoimmune Diagnostics: A Systematic Review on Saliva Testing

International Journal of Environmental Research and Public Health, Journal Year: 2023, Volume and Issue: 20(10), P. 5782 - 5782

Published: May 10, 2023

(1) Background: Immunological laboratory testing is known to be complex, and it usually performed in tertiary referral centers. Many criticalities affect diagnostic immunological testing, such as limited availability, the need for specifically trained staff, potential difficulties collecting blood samples, especially most vulnerable patients, i.e., elderly children. For this reason, identification of a new feasible reliable methodology autoantibody detection urgently needed. (2) Methods: We designed systematic review investigate available literature on utilization saliva samples testing. (3) Results: A total 170 articles were identified. Eighteen studies met inclusion criteria, accounting 1059 patients 671 controls. The collection method was mostly represented by passive drooling (11/18, 61%), frequently described antibody ELISA (12/18, 67%). analysis included 392 with rheumatoid arthritis, 161 systemic lupus erythematosus, 131 type 1 diabetes mellitus, 116 primary biliary cholangitis, 100 pemphigus vulgaris, 50 bullous pemphigoids, 49 Sjogren syndrome, 39 celiac disease, 10 antiphospholipid syndromes, 8 undifferentiated connective tissue 2 sclerosis, autoimmune thyroiditis. majority reviewed involved adequate controls, allowed clear distinction (10/12 studies, 83%). More than half papers showed correlation between serum results (10/18, 55%) detection, varying rates correlation, sensitivity, specificity. Interestingly, many clinical manifestations. (4) Conclusions: Saliva might represent an appealing alternative serum-based considering correspondence Nonetheless, standardization sample processing, maintenance, has yet fully addressed.

Language: Английский

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Parent Attitudes regarding Orthodontists’ Role as Potential Administrators of Human Papilloma Virus (HPV) Vaccines

International Journal of Dentistry, Journal Year: 2022, Volume and Issue: 2022, P. 1 - 7

Published: June 6, 2022

To assess parent attitudes regarding orthodontists' role as potential administrators of human papilloma virus (HPV) vaccines.275 parents adolescents, aged 11-17, who attended the orthodontic clinic at an American university for adjustment visits and met inclusion criteria were given information about HPV vaccines. A paper questionnaire was administered to comfort level with orthodontists vaccinators. Demographic other explanatory characteristics collected. Descriptive, bivariate, multivariate ordinal logistic regression analyses performed SPSS statistical software v25.The majority participants between 31 40 years old, 79.6% identifying female. 54.3% subjects' children identified Although 71.3% Hispanic, 55.3% total chose respond in Spanish. 66.7% reported education high school degree or less. Overall, 52.4% responded that they would be comfortable administering vaccines their children. Bivariate analysis suggested a significant association (p < 0.05) taking survey Spanish parents' educational attainment vaccine administration level. Multivariate indicates (adjusted OR: 2.42, 95% CI: 1.24-4.72; p .01) male 1.66, 1.01-2.73; 047) vaccine.The language influenced vaccinators, having positive correlation Parents more vaccinator.

Language: Английский

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Application of functional proteomics in understanding RNA virus-mediated infection

Advances in protein chemistry and structural biology, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

Language: Английский

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