Scanning electron microscopic screening; Can it be a taxonomic tool for identification of traditional therapeutic plants DOI

Mehreen Jalal,

Shabnum Shaheen,

Zeb Saddiqe

et al.

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 84(4), P. 730 - 745

Published: Oct. 30, 2020

Abstract The limiting factor in the utilization of herbal medicine is risk replacement or substitution their plant resources. Therefore, authentication medicinal plants before its use medicines essentially required. Hence, prime objective this study was to provide some reliable morpho‐anatomical and morpho‐palynological tools for identification nine traditional therapeutic district Lahore. For study, scanning electron microscopic light approaches has been employed. Results reported great variations qualitative quantitative features epidermal cell, stomatal complexes, trichomes, silica bodies oil droplets. example, Aegle marmelos L. can be distinguished from Cestrum diurnum based on cell shapes, as irregular cells former lobed shape later one. Similarly unicellular, bicellular, multicellular trichomes could an basis Cynodon dactylon L., Lantana camara Pongamia pinnata respectively. In combination with these characters, SEM‐based characterization also diagnostic value. Both possessed similar (irregular) but delimited pollen morphology spheroidal whereas prolate . Moreover, characters aid well species delimitation, such found both Terminalia chebula Retz. varied P/E ratio, that is, 0.97 0.88, leaf correlation morph‐palynological traits showed potential taxonomic resolution at level. Research Highlights This provides valuable district, Lahore, Pakistan. Light studies provided useful systematic data studied plants. include moprho‐anatomical keys used future purposes.

Language: Английский

Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity DOI

Nosheen Feroze,

Bushra Arshad, Mohammad Younas

et al.

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(1), P. 72 - 80

Published: Oct. 16, 2019

Nanoparticles as biomedicine has made a crucial role in health biotechnology. Different transition metals various forms playing nanotechnological advances and biological applications. Silver one of the nontoxic, safe inorganic antibacterial agents can serve replacement antibiotics. Present research is based on biogenic synthesis silver nanoparticles (Ag-NPs) potential antibiotics from fungal metabolites Penicillium oxalicum. We used different analytical techniques X-ray diffraction (XRD) scanning electron microscopy (SEM) for characterization biosynthesized nanoparticles. Furthermore, activity was checked against Staphylococcus aureus, S. dysenteriae, Salmonella typhi by using well diffusion method UV visible spectrophotometer. Maximum zone inhibition recorded Shigella dysenteriae 17.5 ± 0.5 mm (mm) both species 18.3 0.60 typhi. The P. oxalicum showed excellent activity. It concluded our results that have significant might be useful wide range applications such bactericidal agent resistant bacteria, preventing infections, healing wounds, anti-inflammation.

Language: Английский

Citations

185
Comparative morphology, anatomy and chemotaxonomy of two Cissus Linn. species DOI
Matthew Chidozie Ogwu, Moses Edwin Osawaru,

Emmanuel Amodu

et al.

Revista Brasileira de Botânica, Journal Year: 2023, Volume and Issue: 46(2), P. 397 - 412

Published: April 19, 2023

Language: Английский

Citations

53
Taxonomic study of subfamily Nepetoideae (Lamiaceae) by polynomorphological approach DOI
Saba Gul, Mushtaq Ahmad, Muhammad Zafar

et al.

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(7), P. 1021 - 1031

Published: March 12, 2019

The present study is insight into pollen morphology for characterizing species and their utility in the taxonomic separation of certain taxa subfamily Nepetoideae (Lamiaceae) from Pakistan. micromorphology 11 was analyzed documented using light microscopy scanning electron (SEM) both qualitative quantitative characteristics. Most have hexazonocolpate grains but trizonocolpate tetrazonocolpate with circular oval amb were also rarely observed Mentha spicata. basic shape most studied subspheroidal prolate M. spicata, S. coccinea, plebeia. exine sculpturing taxonomically very informative particularly at level. Observations SEM revealed various types grains: reticulate, bireticulate, microreticulate, perforate, aerolate, gammate. bireticulate type further subdivided three subtypes based on number secondary lumina each primary lumen characterized by varying characteristics reticulum muri. A significant variation colpus surface ornamentation. maximum polar diameter found O. americanum (58 ± 5.8 μm) equatorial basilicum (50.25 1.37 μm). Pollen features discussed compared current taxonomical concepts. results showed that traits to classify taxa. Furthermore, provide additional evidence distinguish macromorphologically similar other.

Language: Английский

Citations

60
Foliar micromorphology of Convolvulaceous species with special emphasis on trichome diversity from the arid zone of Pakistan DOI
Shomaila Ashfaq, Mushtaq Ahmad, Muhammad Zafar

et al.

Flora, Journal Year: 2019, Volume and Issue: 255, P. 110 - 124

Published: April 25, 2019

Language: Английский

Citations

59
Taxonomic significance of foliar epidermal morphology in Lamiaceae from Pakistan DOI
Saba Gul, Mushtaq Ahmad, Muhammad Zafar

et al.

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(9), P. 1507 - 1528

Published: June 7, 2019

Foliar micromorphological features are useful to elucidate the taxonomy and systematics of Lamiaceae species. Leaf epidermal morphology using scanning electron microscopy light 22 species from 15 genera have been investigated with an aim solve its taxonomic problem in correct identification. Various foliar were observed explain their importance resolving identification taxa. Two main types trichomes observed; glandular (GTs) nonglandular (NGTs). GTs further divided into seven subtypes including capitate, subsessile sessile sunken, barrel, peltate, clavate. Similarly, NGTs also simple unicellular multicellular conical, falcate, cylindrical, dendrite, papillose, short hook shape. Quantitative measurement includes length width trichomes, stomatal complex, cells, stomata, index. Based on characters, a key was developed delimit correctly identify studied Further molecular, other anatomical phylogenetic studies recommended strengthen Lamiaceae.

Language: Английский

Citations

59
Taxonomic study of one generic and two new species record to the flora of Pakistan using multiple microscopic techniques DOI
Saraj Bahadur, Mushtaq Ahmad, Muhammad Zafar

et al.

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(4), P. 345 - 353

Published: Dec. 2, 2019

Abstract Recent field exploration and collections has led to the findings of several new species in Pakistan. Here we reported two Ajuga reptance L Sphagneticola trilobata (L.) Prusk for first time Pakistan flora as these were neither listed any other literature nor identified before These found a result taxonomic studies performed year 2019 District Rawalpindi Islamabad, Microscopic techniques used confirmation foliar epidermal pollen micromorphological features. Detailed study (morphological, palynological, epidermal) was provided correct identification delimitation using both light scanning electron microscopy. Morphological results compared with Taiwan China.

Language: Английский

Citations

41
The quest for some novel antifertility herbals used as male contraceptives in district Shangla, Pakistan DOI
Wajid Zaman, Mushtaq Ahmad, Muhammad Zafar

et al.

Acta Ecologica Sinica, Journal Year: 2019, Volume and Issue: 40(1), P. 102 - 112

Published: May 27, 2019

Language: Английский

Citations

40
Using microscopic techniques for taxonomic implications of seed and fruits of Delphinium L. (sensu lato) (Ranunculaceae) DOI

Anahita Hadidchi,

Farideh Attar, Fazal Ullah

et al.

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 83(2), P. 99 - 117

Published: Nov. 11, 2019

In present research, micromorphological characters of the genus Delphinium L. (sensu lato) Ranunculaceae seeds and fruits were studied using microscopic techniques. A total 37 species light (LM) scanning electron microscopy (SEM) to evaluate characteristics for taxonomic identification relationship in genus. For each taxon, 33 observed studied. All analyzed MVSP software UPGMA method by dissimilarity index (average distance). Considerable differences trichomes shape fruit seed color features. The results revealed that all taxa are separated into three clades. first clade consists D. pallidiflorum semibarbatum, which have black two types glandular crispate fruit. second speciosum, szowitsianum, dasystachyson, aquilegifolium, ursinum, C. trigonelloides, camptocarpa, had a brown seeds, villous on also been observed. remaining classified as third having various colors including yellow, black, trichome shapes included one following forms strigose, villous, less, glandular, hooked, fruits. aim study is use delimitation Delphinium.

Language: Английский

Citations

30
Taxonomic importance of spore morphology in Thelypteridaceae from Northern Pakistan DOI
Syed Nasar Shah, Mushtaq Ahmad, Muhammad Zafar

et al.

Microscopy Research and Technique, Journal Year: 2019, Volume and Issue: 82(8), P. 1326 - 1333

Published: May 14, 2019

Abstract Spore morphology of Thelypteridaceae species growing in Malakand Division, Northern Pakistan, was studied using both light microscopy and scanning electron microscopy. The taxa are Christella dentata Glaphyropteridopsis erubescens the subfamily Thelypteridoideae, Phegopteris connectilis , Pseudophegopteris pyrrhorhachis levingei Phegopteridoideae. exhibit differences spore size, exospore thickness, color, ornamentation. Spores monolete medium‐sized, shape is ellipsoidal polar equatorial views. average measurement diameter ranges from 27 μm to 31 μm, whereas direction it varied 20 40 μm. thickness 1.2 2.4 Reticulate, laevigate with microgranules, cristate, coarsely echinate surface ornamentation observed among species. Multivariate analysis including unweighted pair group method arithmetic mean principal component used for grouping discrimination genera.

Language: Английский

Citations

28
Foliar micromorphology and its role in identification of the Apocynaceae taxa DOI

Kalsoom Bashir,

Aamir Sohail, Usman Ali

et al.

Microscopy Research and Technique, Journal Year: 2020, Volume and Issue: 83(7), P. 755 - 766

Published: March 2, 2020

Abstract In the present study, we evaluate importance of foliar epidermal micromorphological characteristics Apocyanaceae for accurate identification and classification. The species were collected from University Peshawar's main campus in spring season to observe its qualitative quantitative features. length width guard cells, stomatal pore subsidiary trichomes, crypts on both sides leaf examined. Many observed be hypostomatic. Plumeria rubra , Raulfia serpentine Thevetia peruviana Trachelospermum lucidum Alstonia scholaris Catharanthus roseus demonstrated hypostomatic leaves. Nearly all investigated had anisocytic type stomata only or combination with other types upper lower epidermis. Carissa carandas anomocytic, anisocytic, cyclocytic epidermis, epidermis showed variations type, such as stephanocytic, brachyparacytic, hemiparacytic. Nerium oleander no specific shape but which enclosed inside many trichomes. taxonomic key based types, index value, statistical analysis, along shows link between selected plants species, will provide a baseline future anatomical studies. This study highlights undocumented characteristics. this helpful delimitation family Apocynaceae.

Language: Английский

Citations

23