Liquid biopsy at the frontier in renal cell carcinoma: recent analysis of techniques and clinical application

Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)

Published: Feb. 21, 2023

Abstract Renal cell carcinoma (RCC) is a major pathological type of kidney cancer and one the most common malignancies worldwide. The unremarkable symptoms early stages, proneness to postoperative metastasis or recurrence, low sensitivity radiotherapy chemotherapy pose challenge for diagnosis treatment RCC. Liquid biopsy an emerging test that measures patient biomarkers, including circulating tumor cells, cell-free DNA/cell-free DNA, RNA, exosomes, tumor-derived metabolites proteins. Owing its non-invasiveness, liquid enables continuous real-time collection information diagnosis, prognostic assessment, monitoring, response evaluation. Therefore, selection appropriate biomarkers crucial identifying high-risk patients, developing personalized therapeutic plans, practicing precision medicine. In recent years, owing rapid development iteration extraction analysis technologies, has emerged as cost, high efficiency, accuracy clinical detection method. Here, we comprehensively review components their applications over past 5 years. Additionally, discuss limitations predict future prospects.

Language: Английский

---

A critical systematic review of extracellular vesicle clinical trials

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(10)

Published: Sept. 27, 2024

Abstract This systematic review examines the landscape of extracellular vesicle (EV)‐related clinical trials to elucidate field's trends in applications and EV‐related methodologies, with an additional focus on acknowledgement EV subpopulations. By analysing data from public reporting repositories, we catalogued 471 date, indications for over 200 diseases. Diagnostics companion diagnostics represented bulk cancer being most frequent application. therapeutics mainly utilized mesenchymal stromal cell (MSC) EVs were frequently used treatment respiratory illnesses. Ultracentrifugation RNA‐sequencing common isolation characterization techniques; however, methodology each was not reported study records. Most relied isolates, only 11% utilizing subpopulations their experimental design. While this may be connected a lack available techniques suitable implementation, it also highlights opportunity use improve translational efforts. As academic research identifies more chemically distinct technologies enrichment, forecast refined near future. emphasizes need meticulous methodological consideration enhance success EV‐based interventions, pointing towards paradigm shift personalized medicine.

Language: Английский

---

Advances in Extracellular Vesicle Research Over the Past Decade: Source and Isolation Method are Connected with Cargo and Function

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(19)

Published: Jan. 25, 2024

Abstract The evolution of extracellular vesicle (EV) research has introduced nanotechnology into biomedical cell communication science while recognizing what is formerly considered “dust” as constituting an entirely new universe signaling particles. To display the global EV landscape, a systematic review 20 364 original articles selected from all 40 684 EV‐related records identified in PubMed 2013–2022 performed. Machine‐learning used to categorize high‐dimensional data and further dissected significant associations between source, isolation method, cargo, function. Unexpected correlations these four categories indicate prevalent experimental strategies based on cargo connectivity with function interest being associated certain sources or strategies. Conceptually relevant association size‐based protein uptake will guide strategic conclusions enhancing future product development. Based this study, open‐source database built facilitate analysis conventional AI tools identify additional causative interest.

Language: Английский

---

Acoustic separation and concentration of exosomes for nucleotide detection: ASCENDx

Science Advances, Journal Year: 2024, Volume and Issue: 10(10)

Published: March 8, 2024

Efficient isolation and analysis of exosomal biomarkers hold transformative potential in biomedical applications. However, current methods are prone to contamination require costly consumables, expensive equipment, skilled personnel. Here, we introduce an innovative spaceship-like disc that allows Acoustic Separation Concentration Exosomes Nucleotide Detection: ASCENDx. We created ASCENDx use acoustically driven rotation on a spinning droplet generate swift separation concentration exosomes from patient plasma samples. Integrated plasmonic nanostars the enable label-free detection enriched via surface-enhanced Raman scattering. Direct circulating microRNA samples by platform facilitated diagnostic assay for colorectal cancer with 95.8% sensitivity 100% specificity. overcomes existing limitations exosome-based molecular diagnostics holds powerful position future research, precision medicine, point-of-care medical diagnostics.

Language: Английский

---

Plant-Derived Extracellular Vesicles: A New Revolutionization of Modern Healthy Diets and Biomedical Applications

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(6), P. 2853 - 2878

Published: Feb. 1, 2024

Plant-derived extracellular vesicles (PDEVs) have recently emerged as a promising area of research due to their potential health benefits and biomedical applications. Produced by various plant species, these EVs contain diverse bioactive molecules, including proteins, lipids, nucleic acids. Increasing in vitro vivo studies shown that PDEVs inherent pharmacological activities affect cellular processes, exerting anti-inflammatory, antioxidant, anticancer activities, which can potentially contribute disease therapy improve human health. Additionally, efficient biocompatible drug delivery vehicles treating diseases. However, while serve rising star modern healthy diets applications, further is needed address underlying knowledge gaps, especially the lack standardized protocols for isolation, identification, large-scale production. Furthermore, safety efficacy clinical applications must be thoroughly evaluated. In this review, we concisely discuss current PDEV field, characteristics, isolation methods, provide an overview state research. Finally, challenges regarding prospective issues PDEVs. This review expected new insights into vegetables fruits, inspiring advances natural food-based science technology.

Language: Английский

---

Extracellular vesicles as personalized medicine

Molecular Aspects of Medicine, Journal Year: 2022, Volume and Issue: 91, P. 101155 - 101155

Published: Nov. 28, 2022

Extracellular vesicles (EVs) are released from all cells in the body, forming an important intercellular communication network that contributes to health and disease. The contents of EVs cell source-specific, inducing distinct signaling responses recipient cells. specificity their accumulation fluid spaces accessible for liquid biopsies make them highly attractive as potential biomarkers therapies duality favorable (therapeutic) or unfavorable (pathological) messengers is context dependent remains be fully determined homeostasis various disease states. This review describes use biomarkers, drug delivery vehicles, regenerative therapeutics, highlighting examples involving viral infections, cancer, neurological diseases. There growing interest provide personalized therapy based on individual patient characteristics. Increasing evidence suggests EV therapeutic approaches ideal medicine due diversity multifunctionality EVs.

Language: Английский

---

Reproducibility of extracellular vesicle research

European Journal of Cell Biology, Journal Year: 2022, Volume and Issue: 101(3), P. 151226 - 151226

Published: April 15, 2022

Cells release membrane-delimited particles into the environment. These are called "extracellular vesicles" (EVs), and EVs present in fluids contacting cells, including body conditioned culture media. Because change contribute to health disease, have become a hot topic. From thousands of papers now published on annually, one easily gets impression that provide biomarkers for all diseases, carriers relevant biomolecules omnipotent therapeutics. At same time, heterogeneous, elusive difficult study due their physical properties complex composition This overview addresses current challenges encountered when working with EVs, how we envision most these will be overcome near future. Right now, an infrastructure is being developed improve reproducibility EV measurement results. comprises expert task forces International Society Extracellular Vesicles (ISEV) developing guidelines recommendations, instrument calibration, standardized transparent reporting, education. Altogether, developments support credibility research by introducing robust reproducibility, which prerequisite understanding biological significance biomarker potential.

Language: Английский

---

Tumor-derived extracellular vesicles as messengers of natural products in cancer treatment

Theranostics, Journal Year: 2022, Volume and Issue: 12(4), P. 1683 - 1714

Published: Jan. 1, 2022

Extracellular vesicles (EVs) are kinds of two-layer secreted by cells.They play significant roles in mediating component exchange between cells, signal transduction, and pathological development.Among them, the tumor-derived EVs (TDEVs) found related to tumor microenvironment cancer development.TDEVs can be designed as a natural drug carrier with high targeting permeability.In recent years, delivery systems (DDS) based on TDEVs for treatments have received considerable attention.In this review, biological characteristics introduced, especially effect tumor.Furthermore, various approaches constructing DDS summarized.Then we listed examples successfully treatment systems.The use chemical drugs, engineered drugs encapsulated respectively particularly application progress active ingredients traditional Chinese medicine.Finally, article introduces latest clinical research progress, marketed preparations challenges TDEVs.

Language: Английский

---

Towards artificial intelligence-enabled extracellular vesicle precision drug delivery

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 199, P. 114974 - 114974

Published: June 24, 2023

Language: Английский

---

Cascaded microfluidic circuits for pulsatile filtration of extracellular vesicles from whole blood for early cancer diagnosis

Science Advances, Journal Year: 2023, Volume and Issue: 9(16)

Published: April 21, 2023

Tumor-derived extracellular vesicles (EVs) hold the potential to substantially improve noninvasive early diagnosis of cancer. However, analysis nanosized EVs in blood samples has been hampered by lack effective, rapid, and standardized methods for isolating detecting EVs. To address this difficulty, here we use electric-hydraulic analogy design cascaded microfluidic circuits pulsatile filtration via integration a cell-removal circuit an EV-isolation circuit. The device is solely driven pneumatic clock pulse generator, allowing preprogrammed, clog-free, gentle, high-yield, high-purity isolation directly from within 30 minutes. We demonstrate its clinical utility protein markers isolated patient using polyethylene glycol–enhanced thermophoretic aptasensor, with 91% accuracy early-stage breast can have broad applications field EV research.

Language: Английский

---