Key role of environmental competence in successful use of entomopathogenic fungi in microbial pest control

Journal of Pest Science, Journal Year: 2023, Volume and Issue: 97(1), P. 1 - 15

Published: April 28, 2023

Abstract One of the main negative effects climate change on biological pest control is alteration relationships between insect pests and their natural enemies (both entomophagous entomopathogenic). Indeed, environmental conditions can have multiple success when using entomopathogenic fungi (EPF), where conidial depletion, inactivation, loss in virulence infectivity all occur. Appropriate mass production formulation strategies for EPF partially solve these problems. However, only strategy to guarantee high selection environmentally competent fungal strains that are able persist host environment required infection period. This review examines criteria EPF. While UV radiation, followed by humidity temperature, probably most important propagule depletion inactivation factors epigeal habitats, temperature critical reducing hypogeal habitats. In addition, geographical origin other biotic abiotic an impact which may competence selected and, therefore, farmer willingness replace chemicals with mycoinsecticides. To achieve this, it urgent promote development microbial solutions adapted relatively uniform climatic zones through more simplified, targeted, less costly approval authorization.

Language: Английский

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Molecular basis and regulatory mechanisms underlying fungal insecticides' resistance to solar ultraviolet irradiation

Pest Management Science, Journal Year: 2021, Volume and Issue: 78(1), P. 30 - 42

Published: Aug. 16, 2021

Abstract Resistance to solar ultraviolet (UV) irradiation is crucial for field‐persistent control efficacies of fungal formulations against arthropod pests, because their active ingredients are formulated conidia very sensitive UV wavelengths. This review seeks summarize advances in studies aiming quantify, understand and improve conidial resistance. One focus has been on the many sets genes that have revealed postgenomic era contribute or mediate resistance insect pathogens serving as main sources insecticides. Such genetic unveiled broad basis UV‐resistant molecules including cytosolic solutes, cell wall components, various antioxidant enzymes, numerous effectors signaling proteins, function developmental, biosynthetic stress‐responsive pathways. Another molecular regulatory mechanisms underlying photorepair UV‐induced DNA lesions photoreactivation UV‐impaired conidia. Studies shed light upon a photoprotective mechanism depending not only one two photorepair‐required photolyases, but also white collar proteins other partners play similar more important roles via interactions with photolyases. Research hotspots suggested explore network photoprotection development application strategies © 2021 Society Chemical Industry.

Language: Английский

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Two white collar proteins protect fungal cells from solar UV damage by their interactions with two photolyases in Metarhizium robertsii

Environmental Microbiology, Journal Year: 2021, Volume and Issue: 23(9), P. 4925 - 4938

Published: Jan. 15, 2021

The photolyases PHR1 and PHR2 enable photorepair of fungal DNA lesions in the forms UV-induced cyclobutane pyrimidine dimer (CPD) (6-4)-pyrimidine-pyrimidone (6-4PP) photoproducts, but their regulation remains mechanistically elusive. Here, we report that white collar proteins WC1 WC2 mutually interacting to form a light-responsive transcription factor regulate photolyase expression required for UV resistance insect-pathogenic fungus Metharhizum robertsii. Conidial UVB decreased by 54% Δwc1 67% Δwc2. Five-hour exposure UVB-inactivated conidia visible light resulted photoreactivation rates 30% 9% Δwc2 mutants, contrasting 79%-82% wild-type complemented strains. Importantly, abolished phr1 Δwc-2 phr2 incapable CDP 6-4PP UVB-impaired cells respectively. Yeast two-hybrid assays revealed interactions either WC protein with both PHR2. Therefore, essential roles UVB-induced rely upon with, hence transcriptional activation of, These findings uncover novel WC-cored pathway mediates filamentous response adaptation solar irradiation.

Language: Английский

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Comparative roles of three adhesin genes (adh1–3) in insect-pathogenic lifecycle of Beauveria bassiana

Applied Microbiology and Biotechnology, Journal Year: 2021, Volume and Issue: 105(13), P. 5491 - 5502

Published: June 25, 2021

Language: Английский

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Do the beneficial fungi manage phytosanitary problems in the tea agro-ecosystem?

BioControl, Journal Year: 2021, Volume and Issue: 66(4), P. 445 - 462

Published: March 15, 2021

Language: Английский

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Current developments in the resistance, quality, and production of entomopathogenic fungi

World Journal of Microbiology and Biotechnology, Journal Year: 2022, Volume and Issue: 38(7)

Published: May 18, 2022

Language: Английский

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Optional strategies for low‐risk and non‐risk applications of fungal pesticides to avoid solar ultraviolet damage

Pest Management Science, Journal Year: 2022, Volume and Issue: 78(11), P. 4660 - 4667

Published: July 22, 2022

Solar ultraviolet (UV) irradiation is harmful to formulated conidia as active ingredients of fungal pesticides and hence restrains their field application in sunny days summer, a season requiring frequent pest controls. This conflict makes it necessary explore optimal strategies for the suppress populations but avoid solar UV damage during summer.The Beauveria bassiana, wide-spectrum pesticide, were tolerable UVB (major wavelengths) ≤0.5 J cm-2 . The this upper limit caused loss conidial viability infectivity if not photoreactivated by light exposure after irradiation. Intriguingly, resulted high photoreactivation rate UVB-inactivated an insignificant or marginal difference insecticidal activity between normal those photoreactivated. Modeling analysis intensity recorded hourly over daylight five summer from 5:00 am 7:00 pm at 30° 17'57'' N 120°5'7'' E revealed variation daily accumulated dose 2.07 2.78 , which was far beyond limit. A more ~0.2 appeared 3:00 pm, no pm.Fungal tolerance, capability accumulation pattern are based propose optional strategy low-risk non-risk summer. © 2022 Society Chemical Industry.

Language: Английский

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Timing of Fungal Insecticide Application to Avoid Solar Ultraviolet Irradiation Enhances Field Control of Rice Planthoppers

Insects, Journal Year: 2023, Volume and Issue: 14(4), P. 307 - 307

Published: March 23, 2023

Thechemical control of rice planthoppers (RPH)is prohibited in annual rice–shrimp rotation paddy fields. Here, the fungal insecticides Beauveria bassiana ZJU435 and Metarizhium anisoplae CQ421 were tested for RPH populations dominated by Nilaparvata lugens three field trials. During four-week trials initiated from harsh weather high temperatures strong sunlight, crop at stages tillering to flowering was effectively protected sprays applied 14-day intervals. The either insecticide after 5:00 p.m. (solar UV avoidance) suppressed population better than those before 10 a.m. avoidance versus exposure resulted mean efficacies 60% 56% 41% 45% on day 7, 77% 78% 63% 67% 14, 84% 82% 80% 79% 21, 81% 75 28, respectively. These results indicate that can fields offer a novel insight into significance solar-UV-avoiding application improved pest during sunny summers.

Language: Английский

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Roles of six Hsp70 genes in virulence, cell wall integrity, antioxidant activity and multiple stress tolerance of Beauveria bassiana

Fungal Genetics and Biology, Journal Year: 2020, Volume and Issue: 144, P. 103437 - 103437

Published: July 24, 2020

Language: Английский

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Roles of BrlA and AbaA in Mediating Asexual and Insect Pathogenic Lifecycles of Metarhizium robertsii

Journal of Fungi, Journal Year: 2022, Volume and Issue: 8(10), P. 1110 - 1110

Published: Oct. 21, 2022

BrlA and AbaA are key activators of the central developmental pathway (CDP) that controls asexual development in Aspergillus but their roles remain insufficiently understood hypocerealean insect pathogens. Here, regulatory orthologs Metarhizium robertsii (Clavicipitaceae) were characterized for comparison to those elucidated previously Beauveria bassiana (Cordycipitaceae) at phenotypic transcriptomic levels. Time-course transcription profiles brlA, abaA, other CDP activator gene wetA revealed they not so sequentially activated M. as learned Aspergillus. Aerial conidiation essential fungal infection dispersal, submerged blastospore production mimicking yeast-like budding proliferation hemocoel, pathogenicity via cuticular penetration all abolished a consequence brlA or abaA disruption, which had little impact on normal hyphal growth. The disruptants severely compromised virulence cuticle-bypassing (intrahemocoel injection) differentially impaired cellular tolerance oxidative cell wall-perturbing stresses. ΔbrlA ΔabaA mutant shad 255 233 dysregulated genes (up/down ratios: 52:203 101:122) respectively, including 108 co-dysregulated. These counts small compared with 1513 2869 707:806 1513:1356) identified mutants B. bassiana. Results only conserved control also indispensable adaptation insect-pathogenic lifecycle host habitats. Intriguingly, BrlA- AbaA-controlled expression networks largely different between two pathogens, similar phenotypes absence either abaA.

Language: Английский

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