Arabian Journal for Science and Engineering, Journal Year: 2024, Volume and Issue: unknown
Published: Aug. 8, 2024
Language: Английский
Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(3)
Published: Feb. 14, 2024
Abstract A biofilm is a collection of microorganisms organized in matrix extracellular polymeric material. Biofilms consist microbial cells that attach to both surfaces and each other, whether they are living or non-living. These biofilms can lead hospital-acquired infections generally detrimental. They possess the ability resist human immune system antibiotics. The National Institute Health (NIH) states formation associated with 65% all illnesses 80% chronic illnesses. Additionally, non-device-related include conditions like cystic fibrosis, otitis media, infective endocarditis, inflammatory disorders. This review aims provide an overview research on caused by biofilms, methods used for detection, recent approaches combat future perspectives, including development innovative antimicrobial strategies such as peptides, bacteriophages, agents disrupt biofilms.
Language: Английский
Citations
28
Biomedicines, Journal Year: 2023, Volume and Issue: 11(5), P. 1442 - 1442
Published: May 14, 2023
The repurposing of drugs is one the most competent strategies for discovering new antimicrobial agents. Vildagliptin a dipeptidyl peptidase-4 inhibitor (DPI-4) that used effectively in combination with metformin to control blood glucose levels diabetic patients. This study was designed evaluate anti-virulence activities this against clinically important pathogens, Pseudomonas aeruginosa. current findings show significant ability vildagliptin-metformin diminish biofilm formation, bacterial motility, and production virulent extracellular enzymes pyocyanin pigment. Furthermore, drug significantly increased susceptibility P. aeruginosa oxidative stress, indicating immunity enhancement eradication cells. In compliance vitro findings, histopathological photomicrographs mice showed considerable protective effect metformin-vildagliptin aeruginosa, revealing relief inflammation due aeruginosa-induced pathogenesis. mainly employs quorum sensing (QS) systems its huge arsenal virulence factors. can be interrupted by anti-QS both vildagliptin, as exhibited affinity QS receptors. Additionally, downregulated expression main three QS-encoding genes These at very low concentrations (10, 1.25 mg/mL, respectively) compared (850, 50 diabetes.
Language: Английский
Citations
25
Saudi Pharmaceutical Journal, Journal Year: 2024, Volume and Issue: 32(5), P. 102041 - 102041
Published: March 18, 2024
The rise of antibiotic resistance in bacteria is becoming a global concern, particularly due to the dwindling supply new antibiotics. This situation mandates discovery antimicrobial candidates. Plant-derived natural compounds have historically played crucial role development antibiotics, serving as rich source substances possessing properties. Numerous studies supported reputation 6-gingerol, prominent compound found ginger family, for its antibacterial In this study, activities 6-gingerol were evaluated against Gram-negative bacteria, Acinetobacter baumannii and Klebsiella pneumoniae, with particular focus on clinically significant Pseudomonas aeruginosa Gram-positive Staphylococcus aureus. Furthermore, anti-virulence assessed vitro, vivo, silico. current findings showed that 6-gingerol's activity effect disruption bacterial cell membrane efflux pumps, it significantly decreased disrupted S. aureus P. aeruginosa. biofilm formation production virulence factors concentrations below MICs. properties could be attributed capacity disrupt virulence-regulating systems; quorum sensing (QS). 6-Gingerol was interact QS receptors downregulate genes responsible QS. addition, molecular docking, dynamics (MD) simulation results indicated comparable binding affinity co-crystalized ligands different targets well stable interactions during 100 ns MD simulations. These suggest holds promise an agent can combined antibiotics treatment severe infections.
Language: Английский
Citations
13
Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)
Published: Jan. 5, 2024
Abstract With the inappropriate use of antibiotics, antibiotic resistance has emerged as a major dilemma for patients infected with Pseudomonas aeruginosa . Elastase B (LasB), crucial extracellular virulence factor secreted by P. , been identified key target antivirulence therapy. Quercetin, natural flavonoid, exhibits promising potential an agent. We aim to evaluate impact quercetin on LasB and elucidate underlying mechanism. Molecular docking molecular dynamics simulation revealed rather favorable intermolecular interaction between LasB. At sub-MICs ≤256 μg/ml, was found effectively inhibit production activity elastase, well downregulate transcription level lasB gene in both PAO1 clinical strains Through correlation analysis, significant positive correlations were shown QS system regulatory genes lasI lasR rhlI rhlR Then, we expression significantly deficient ΔlasI ΔlasIΔrhlI mutants. In addition, downregulated levels regulated rhlI, pqsA pqsR attenuated synthesis signaling molecules 3-oxo-C12-HSL C4-HSL PAO1. Quercetin also able compete ligands OdDHL, BHL, PQS binding receptor proteins LasR, RhlR, PqsR, respectively, resulting formation more stabilized complexes. Taken together, enormous combating disrupting vitro thereby offering alternative approach therapy infections. Key points • diminished content elastase aeruginosa. inhibited acted based system.
Language: Английский
Citations
12
Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15
Published: April 5, 2024
Proteus mirabilis is a Gram-negative bacterium with exclusive molecular and biological features. It versatile pathogen acclaimed for its distinct urease production, swarming behavior, rapid multicellular activity. Clinically, P. frequent of the human urinary system where it causes tract infections (UTIs) catheter-associated (CAUTIs). This review explores epidemiology, risk factors, clinical manifestations, treatment infections, emphasizing association UTIs. The bacterium’s genome analysis revealed presence resistance genes against commonly used antibiotics, an antibiotic-resistant phenotype that poses serious challenge. Particularly, emergence extended-spectrum β-lactamases (ESBLs) carbapenemases resistant strains. On level, possesses wide array virulence factors including production fimbriae, urease, hemolysins, metallophores, biofilm formation. thoroughly tackles substantial gap in understanding role metallophores shaping virulence. Siderophores, iron metal chelating transporting particularly contribute to complex pathogenic strategies, displaying potential target therapeutic intervention.
Language: Английский
Citations
12
Nanotechnology Reviews, Journal Year: 2024, Volume and Issue: 13(1)
Published: Jan. 1, 2024
Abstract Bacterial biofilms contribute to increased pathogenesis and bacterial resistance. Biofilms can enhance pathogenicity by shielding bacteria from the immune system antibiotics, they are associated with persistent infections. Additionally, antibiotic resistance mechanisms within make them challenging treat, emphasizing need for strategies be addressed. Mitigating virulence is a promising strategy that could ease their eradication host immunity without stressing induce The merits of this augmented when using safe anti-virulence candidates in proper formulations. current study aimed evaluate antibiofilm efficacy curcumin–zein nanoparticles against Pseudomonas aeruginosa . In vitro investigations were performed assess effect on biofilm formation, motility, production factors, including proteases, hemolysins, pyocyanin, comparison bulk curcumin. Furthermore, expression genes encode quorum sensing (QS) systems regulate was assessed. An silico done affinity curcumin QS receptors. an vivo protection assay inhibitory our preparation diminishing P. aeruginosa’s capacity pathogenesis. results showed significant activities compared These attributed curcumin’s interfering its virulence. conclusion, acquires anti-QS, anti-virulence, vastly enhanced upon loading zein nanoparticles.
Language: Английский
Citations
11
Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(3)
Published: Feb. 27, 2024
Language: Английский
Citations
8
Critical Reviews in Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 15
Published: Feb. 20, 2025
Quorum sensing (QS) is a bacterial communication method closely linked with population density and regulates biofilm formation the secretion of virulence factors through release, recognition, prompt response to small molecule signals. At low cell density, each bacterium produces concentration QS signals that diffuse or are actively transported into external environment. The accumulated in environment reach threshold when attains certain enabling effective recognition interaction their receptors. This leads coordinated gene expression various biological activities across population. Targeting system presents promising strategy hinder factor secretion, providing potential approach control growth reproduction. study aims analyze intercellular mechanisms quorum quenching (QQ), which focuses on disrupting signal molecules keep below preventing specific pathogenic factors. applications QQ different fields also reviewed, underscoring its as novel treatment for infections.
Language: Английский
Citations
1
Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15
Published: March 12, 2024
The development of resistance to carbapenems in Klebsiella pneumoniae due the production metallo-β-lactamases (MBLs) is a critical public health problem because are last-resort drugs used for treating severe infections extended-spectrum β-lactamases (ESBLs) producing K. . Restoring activity by inhibition valuable approach combat carbapenem resistance. In this study, two well-characterized clinical multidrug and carbapenem-resistant isolates were used. sub-inhibitory concentrations pantoprazole well-reported metallo-β-lactamase inhibitor captopril inhibited hydrolytic activities metallo-β-lactamases, with having more inhibiting activities. Both drugs, when combination meropenem, exhibited synergistic Pantoprazole could also downregulate expression genes bla NDM VIM A docking study revealed that bind chelate zinc ions New Delhi Verona integron-encoded MBL (VIM) enzymes higher affinity than control drug comparable natural ligand indicating significant inhibitory against metallo-β-lactamases. conclusion, can be meropenem as new strategy serious caused
Language: Английский
Citations
7
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: April 4, 2024
Pseudomonas aeruginosa belongs to the critical pathogens that represent a global public health problem due their high rate of resistance as listed by WHO. P. can result in many nosocomial infections especially individuals with compromised immune systems. Attenuating virulence factors interference quorum sensing (QS) systems is promising approach treat -resistant infections. Thymoquinone natural compound isolated from Nigella sativa (black seed) essential oil. In this study, minimum inhibitory concentration thymoquinone was detected followed investigating antibiofilm and antivirulence activities subinhibitory against PAO1. The effect on expression QS genes assessed quantitative real-time PCR, protective pathogenesis PAO1 mice mouse survival test. significantly inhibited biofilm, pyocyanin, protease activity, swarming motility. At molecular level, markedly downregulated lasI , l asR rhlI rhlR . Moreover, could protect pathologic effects increasing 20% 100%. conclusion, agent be used an adjunct therapeutic antibiotics attenuate pathogenicity
Language: Английский
Citations
7