Biological processes and factors involved in soft and hard tissue healing

Periodontology 2000, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 20, 2024

Abstract Wound healing is a complex and iterative process involving myriad cellular biologic processes that are highly regulated to allow satisfactory repair regeneration of damaged tissues. This review intended be an introductory chapter in volume focusing on the use platelet concentrates for tissue regeneration. In order fully appreciate clinical utility these preparations, sound understanding factors involved soft hard healing. encompasses appreciation biological mediators both tissues general as well specific consideration periodontal light good advances this basic knowledge, there have been improvements strategies therapeutic management wound The offers one such strategy based principles discussed review.

Language: Английский

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Antibiotic-Loaded Platelet-Rich Fibrin (AL-PRF) as a New Carrier for Antimicrobials: A Systematic Review of In Vitro Studies

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2140 - 2140

Published: Feb. 27, 2025

Platelet-rich fibrin (PRF) has emerged as a promising scaffold for drug delivery, particularly in the context of antimicrobial therapies. This systematic review evaluates incorporation antibiotics into PRF to determine its efficacy localized delivery system compared plain without antibiotics. A was conducted according Preferred Reporting Items Systematic Reviews and Meta-Analyses (PRISMA) guidelines, including 13 vitro studies with moderate risk bias. Antibiotics were incorporated using different methodologies, systemic administration before blood collection, addition centrifugation, injection formed matrices. Outcomes analyzed regarding antibacterial efficacy, structural integrity PRF, release kinetics. Antibiotic-enhanced demonstrated significant activity against various bacterial strains. The enhanced dependent on type antibiotic, concentration, method. Encapsulation approaches facilitated sustained antibiotic release, while higher concentrations occasionally disrupted integrity. Systemic collection enriches effectively, producing inhibition zones. effects outperformed alternative carriers, such collagen sponges. Antibiotic-loaded is potent tool applications clinical settings. Further research needed standardize preparation protocols explore impact methods PRF's regenerative properties.

Language: Английский

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Barrier Membrane in Regenerative Therapy: A Narrative Review

Membranes, Journal Year: 2022, Volume and Issue: 12(5), P. 444 - 444

Published: April 20, 2022

Guided bone and tissue regeneration remains an integral treatment modality to regenerate surrounding teeth dental implants. Barrier membranes have been developed produced commercially allow space for prevent the migration of unwanted cells. Ideal membrane properties, including biocompatibility, sufficient structural integrity suitable shelf life with easy clinical application, are important ensure good regenerative outcomes. Membranes various types, their application depends on origin, material, structure properties. This narrative review aims describe currently available barrier in terms history, main features, indication classify them into groups. Various membranes, those which resorbable non-resorbable, synthetic, added growth factors composed modern materials, such as high-grade polymer (Polyetheretherketone), explored this review.

Language: Английский

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A comprehensive review of platelet-rich plasma for the treatment of dermatologic disorders

Journal of Dermatological Treatment, Journal Year: 2022, Volume and Issue: 34(1)

Published: Nov. 1, 2022

Platelet-rich plasma (PRP) offers anti-inflammatory and regenerative properties through angiogenesis, cell differentiation, proliferation. Although studied in many dermatologic conditions, its efficacy is not well-understood. Our objective to review the use effectiveness of PRP for conditions. A literature search was performed PubMed yielded 54 articles published between January 2000 November 2021; written English were reviewed. Intradermal injections associated with increased hair density androgenic alopecia. Successful treatment inflammatory nail diseases has been reported. Improvement psoriasis described, but only two studies available. higher patient self-assessment scores photoaging fine lines. Treatment melasma improved subjective satisfaction, measures disease improvement. can serve as a safe potentially effective adjunct loss, vitiligo, nonhealing wounds, photoaging, acne scars. An important barrier interpreting research lack standardization preparation protocols, inconsistent clinical endpoints, frequent combination treatments. However, relatively noninvasive, well-established safety profile, satisfaction often high patients perceive great benefit from PRP.

Language: Английский

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Can platelet-rich fibrin act as a natural carrier for antibiotics delivery? A proof-of-concept study for oral surgical procedures

BMC Oral Health, Journal Year: 2023, Volume and Issue: 23(1)

Published: March 9, 2023

Evaluate the role of platelet-rich fibrin (PRF) as a natural carrier for antibiotics delivery through analysis drug release and antimicrobial activity.PRF was prepared according to L-PRF (leukocyte- fibrin) protocol. One tube used control (without drug), while an increasing amount gentamicin (0.25 mg, G1; 0.5 G2; 0.75 G3; 1 G4), linezolid (0.5 L1; L2; 1.5 L3; 2 L4), vancomycin (1.25 V1; 2.5 V2; 3.75 V3; 5 V4) added other tubes. At different times supernatant collected analyzed. Strains E. coli, P. aeruginosa, S. mitis, H. influenzae, pneumoniae, aureus were assess effect PRF membranes with same compared PRF.Vancomycin interfered formation. Gentamicin did not change physical properties released from in time intervals examined. The inhibition area showed that had slight antibacterial activity against all tested microorganisms. Gentamicin-PRF massive Results similar linezolid-PRF, except its coli aeruginosa comparable PRF.PRF loaded allowed drugs effective concentration. Using after oral surgery may reduce risk post-operative infection, replace or enhance systemic antibiotic therapy preserving healing PRF. Further studies are needed prove represents topical tool surgical procedures.

Language: Английский

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Optimization of platelet‐rich fibrin

Periodontology 2000, Journal Year: 2023, Volume and Issue: 94(1), P. 79 - 91

Published: Sept. 8, 2023

Abstract The use of platelet‐rich fibrin (PRF) has gained tremendous popularity in recent years owing to its ability speed wound healing postsurgery. However, date, many clinicians are unaware methods designed optimize the technology. This overview article will discuss advancements and improvements made over aimed at maximizing cell growth factor concentrations. First, a general understanding explaining differences between RPM RCF (g‐force) is introduced. Then, low‐speed centrifugation concept, fixed angle versus horizontal centrifugation, maximize platelet concentrations using optimized protocols be discussed detail. Thereafter, importance chemically modified PRF tubes without addition chemical additives, as well regulation temperature induce/delay clotting, thoroughly described. first kind summarizing all literature on production for clinical treatment.

Language: Английский

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Optimized bone grafting

Periodontology 2000, Journal Year: 2023, Volume and Issue: 94(1), P. 143 - 160

Published: Aug. 23, 2023

Abstract Bone grafting is routinely performed in periodontology and oral surgery to fill bone voids. While autogenous considered the gold standard because of its regenerative properties, allografts xenografts have more commonly been utilized owing their availability as well differential regenerative/biomechanical properties. In particular, are sintered at high temperatures, which allows for slower degradation resorption rates and/or nonresorbable features. As a result, clinicians combined with other classes grafts (most notably autografts various ratios) procedures requiring better long‐term stability, such contour grafting, sinus elevation procedures, vertical augmentations. This review addresses properties each class then highlights importance understanding biomechanical clinical applications, including extraction site management, augmentation, horizontal augmentation procedures. Thereafter, an introduction toward novel production (NRBAs) via high‐temperature sintering presented. These NRBAs not only pose advantage being biocompatible than origin (human vs. animal bone) but also display similar those xenografts. Thus, while packaging premixtures specific indications has never permitted cross‐species contamination FDA/CE requirements, discovery premixing ratios without regulatory restrictions. Therefore, can be produced (e.g., 1:1 ratio allograft/xenograft mixture grafting) need purchasing separate grafts. optimized form could theoretically provide precise purchase future potential simplified implant dentistry.

Language: Английский

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Advances in Regenerative Dentistry Approaches: An Update

International Dental Journal, Journal Year: 2023, Volume and Issue: 74(1), P. 25 - 34

Published: Aug. 2, 2023

Regenerative dentistry is a rapidly evolving field in dentistry, which has been driven by advancements biomedical engineering research and the rising treatment expectations demands that exceed scope of conventional approaches. Tissue engineering, foundation regenerative mainly focuses on 3 key components: stem cells, bioactive molecules, scaffolds. Dental tissue-derived cells are especially significant this regard due to their remarkable properties. techniques have provided novel approaches many strategies various disciplines dentistry. For instance, endodontic procedures such as pulp revascularisation an alternative approach root canal treatment. In addition, surgical nonsurgical periodontal being taken over modified guided tissue regeneration with aid 3-dimensional bioprinting computer-aided design, revolutionised oral maxillofacial engineering. This review presents concise overview latest emerged into clinical practice, potential future technologies, role dental

Language: Английский

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Ten years of injectable platelet‐rich fibrin

Periodontology 2000, Journal Year: 2023, Volume and Issue: 94(1), P. 92 - 113

Published: Nov. 30, 2023

Abstract The use of platelet‐rich fibrin (PRF) has seen widespread advantages over plasma (PRP) in many fields medicine. However, until 2014, PRF remained clinically available only its solid clotted form. Modifications to centrifugation protocols and tube technology have led the development a liquid injectable version (i‐PRF). This narrative review takes look back at technological developments made throughout past decade further elaborates on their future clinical applications. Topics covered include improvements isolation techniques protocols, ways concentrate i‐PRF, impact relevance cooling i‐PRF. Next, various uses i‐PRF are discussed, including regenerative periodontology, implantology, endodontics, temporomandibular joint injections, orthodontic tooth movement. Furthermore, indications medicine also covered, sports injuries osteoarthritis joints, treatment diabetic ulcers/wound care, facial esthetics hair regrowth. Finally, applications mainly as drug delivery vehicle for small biomolecules, such growth factors, antibiotics, exosomes, other medications that may benefit from controlled gradual release biomolecules time.

Language: Английский

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From Basic Science to Clinical Practice: A Review of Current Periodontal/Mucogingival Regenerative Biomaterials

Advanced Science, Journal Year: 2024, Volume and Issue: 11(17)

Published: Feb. 21, 2024

Abstract Periodontitis is a dysbiosis‐driven inflammatory disease affecting the tooth‐supporting tissues, characterized by their progressive resorption, which can ultimately lead to tooth loss. A step‐wise therapeutic approach employed for periodontitis. After an initial behavioral and non‐surgical phase, intra‐bony or furcation defects may be amenable regenerative procedures. This review discusses technologies periodontal regeneration, highlighting current limitations future research areas. The search, performed on MEDLINE database, has identified available biomaterials, including biologicals (autologous platelet concentrates, hydrogels), bone grafts (pure putty), membranes. Biologicals have been critically analyzed in terms of composition, mechanism action, clinical applications. Although certain degree regeneration predictable class II defects, complete defect closure hardly achieved. Moreover, treating III remains challenging. key properties required functional are discussed, none commercially biomaterials possess all ideal characteristics. Therefore, needed promote advancement more effective targeted therapies Lastly, improving design reporting studies suggested strictly adhering Consolidated Standards Reporting Trials (CONSORT) 2010 statement.

Language: Английский

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