Nicotinamide mononucleotide biosynthesis and the F-actin cytoskeleton regulate spindle assembly and oocyte maturation quality in post-ovulatory aged porcine oocytes

Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 17, 2025

Post-ovulatory aging (POA) is associated with reduced fertilization rates and poor embryo quality both in vivo vitro. However, the relationship between nicotinamide adenine dinucleotide (NAD+) filamentous actin (F-actin) cytoskeleton POA-induced oocytes remains unknown. Here, we investigated mechanisms by which NAD+ salvage pathways function oocyte maturation upon POA through F-actin cytoskeleton. Porcine were aged extending vitro (IVM) for an additional 24 h to create a model. adducin 1 (ADD1)-related spindle assembly analyzed using immunofluorescence, western blotting, RNA sequencing identify key gene categories IVM groups. To assess restoring maturation, mononucleotide (NMN) was added efficiency evaluated. Expression of factors, markers, NAD+-related genes via quantitative polymerase chain reaction, blotting. We revealed unique interactions F-actin/ADD1-related factors (clusterin (CLU) FAM111 trypsin-like peptidase A (FAM111A)) poor-quality oocytes. established extension based on 44 IVM. They exhibited collapses abnormal cortical F-actin, ADD1, acetyl(Ac)-α-tubulin protein levels, resulted defective assembly. analysis performed differentially expressed involved viability response aging, cytoskeleton, NAD metabolic processes and/or This showed that NAD-binding after induction, eight downregulated compared Importantly, activation addition NMN medium at rescued capability perturbations F-actin. polymerization generated from essential factor determining quality. effect mediated microtubules related

Language: Английский

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Day after rescue ICSI: eliminating total fertilization failure after conventional IVF with high live birth rates following cryopreserved blastocyst transfer

Human Reproduction, Journal Year: 2023, Volume and Issue: 38(7), P. 1277 - 1283

Published: May 19, 2023

What is the impact of day after rescue ICSI (r-ICSI) on success fresh and frozen embryo transfers?The use r-ICSI can virtually allay fears total fertilization failure (TFF) conventional IVF (C-IVF) achieve high live birth rates blastocyst transfer.More infertility clinics have resorted to in place C-IVF treatment owing fear TFF or a low rate. has been attempted either after. Day proved unsuccessful past.A retrospective data analysis was performed 16 608 qualifying cases between April 2010 July 2021 conducted at single private academically affiliated fertility clinic.r-ICSI principally patients with >4 metaphase II oocytes, showing no signs 18 h C-IVF. who had million motile sperm preparation. then 18-24 insemination, using sample from previous day. rates, cryopreservation cleavage blastocysts embryos, pregnancy transfer were assessed.r-ICSI 377 (2.3% eligible retrieval cycles) mean (±SD) female male age 35.9 ± 4.5 38.1 9.1 years, respectively. A 5459 oocytes initially retrieved. Of undergoing r-ICSI, 2389 (49.5%) fertilized normally, 205 (54.4%) underwent transfer. The 23/186 (12.3%) for 5/19 (26.3%) stage transfers. In 145 cycles frozen, 137 transfers 64/137 (46.7%) receiving only, 25 failed any fertilization, reducing 25/16 (0.15%).This single-center study specific subset patients, which may limit its generalizability other clinics.r-ICSI allows second opportunity fertilize despite poor initial outcomes. Patients achieved indicating that resynchronization endometrium optimize cases. allays when C-IVF, providing evidence overuse without factor not be warranted.The internally funded by Boston IVF. authors declare they conflict interest relation published article.N/A.

Language: Английский

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Cordycepin delays postovulatory aging of oocytes through inhibition of maternal mRNAs degradation via DCP1A polyadenylation suppression

Cellular and Molecular Life Sciences, Journal Year: 2023, Volume and Issue: 80(12)

Published: Nov. 25, 2023

Abstract Postovulatory aging leads to the decline in oocyte quality and subsequent impairment of embryonic development, thereby reducing success rate assisted reproductive technology (ART). Potential preventative strategies preventing oocytes from associated underlying mechanisms warrant investigation. In this study, we identified that cordycepin, a natural nucleoside analogue, promoted vitro, as indicated by reduced fragmentation, improved spindle/chromosomes morphology mitochondrial function, well increased developmental competence. Proteomic RNA sequencing analyses revealed cordycepin inhibited degradation several crucial maternal proteins mRNAs caused aging. Strikingly, was found suppress elevation DCP1A protein inhibiting polyadenylation during postovulatory aging, consequently impeding decapping mRNAs. humans, total mRNA also cordycepin. Collectively, our findings demonstrate prevents mammalian inhibition via suppressing mRNA, promoting

Language: Английский

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Cumulus Cells Accelerate Postovulatory Oocyte Aging through IL1–IL1R1 Interaction in Mice

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(4), P. 3530 - 3530

Published: Feb. 9, 2023

The oocytes of female mammals will undergo aging after ovulation, also known as postovulatory oocyte (POA). Until now, the mechanisms POA have not been fully understood. Although studies shown that cumulus cells accelerate over time, exact relationship between two is still unclear. In study, by employing methods mouse and transcriptome sequencing experimental verification, we revealed unique characteristics through ligand–receptor interactions. results indicate activated NF-κB signaling in IL1–IL1R1 interaction. Furthermore, it promoted mitochondrial dysfunction, excessive ROS accumulation, increased early apoptosis, ultimately leading to a decline quality appearance POA. Our role accelerating POA, this result lays foundation for an in-depth understanding molecular mechanism Moreover, provides clues exploring oocytes.

Language: Английский

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Kaempferol ameliorates in-vitro and in-vivo postovulatory oocyte ageing in mice

Reproductive BioMedicine Online, Journal Year: 2022, Volume and Issue: 45(6), P. 1065 - 1083

Published: July 19, 2022

Language: Английский

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Lycopene Reduces the In Vitro Aging Phenotypes of Mouse Oocytes by Improving Their Oxidative Status

Veterinary Sciences, Journal Year: 2022, Volume and Issue: 9(7), P. 336 - 336

Published: July 1, 2022

Postovulatory aging is a major problem that limits the success of many assisted reproductive technologies (ARTs). Oxidative stress leading cause oocyte aging. This study investigated effects lycopene supplementation in vitro maturation (IVM) medium during mouse oocytes on oocytes' morphology and oxidative status. Mouse cumulus-oocyte complexes (COCs) were collected cultured IVM either for 17 h, (freshly matured oocytes), or 48 (in vitro-aged with without lycopene. The rate fragmented degenerated levels hydrogen peroxide (H

Language: Английский

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Protocatechuic Acid Delays Postovulatory Oocyte Ageing in Mouse

Molecular Nutrition & Food Research, Journal Year: 2022, Volume and Issue: 67(4)

Published: Dec. 20, 2022

Tea is a popular beverage worldwide and has many health functions. Protocatechuic acid (PCA) an important bioactive component of tea benefit to health. In some cases, oocytes after ovulation may miss the optimal fertilization time enter postovulatory ageing process. Therefore, investigate role PCA in delaying oocyte aimed.Metaphase II (MII) aged vitro are randomly divided into three groups: control, aged, + PCA. treatment (30 µM) reduces fragmentation rate incidence abnormal spindle morphology chromosome misalignment 24 h vitro. The mitochondrial dysfunction oocytes, such as decreased membrane potential excessive accumulation reactive oxygen (ROS), also alleviated by delays apoptosis improves sperm binding capacity. Otherwise, treated with have higher blastocyst compared untreated vitro.PCA ingredient that quality, suggesting available improve quality

Language: Английский

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Ferulic acid ameliorates the quality of in vitro-aged bovine oocytes by suppressing oxidative stress and apoptosis

Aging, Journal Year: 2023, Volume and Issue: 15(21), P. 12497 - 12512

Published: Nov. 8, 2023

Ferulic acid (FA) is a well-known natural antioxidant that scavenges oxygen free radicals and alleviates oxidative stress. This study investigated the chemopreventive potential of FA against bovine oocyte quality decline during in vitro aging. The results showed 5 μM supplementation decreased abnormality rate vitro-aged oocytes. In addition, effectively improved capacity by removing excessive ROS maintaining intracellular GSH levels enzyme activity. mitochondrial activity, membrane ATP aged oocytes were obviously enhanced supplementation. Furthermore, reduced aging-induced DNA damage maintained stability Moreover, sperm binding assay number bound to zona pellucida on was significantly higher supplemented group than Aged group. Therefore, beneficial for could become preventing aging maturation.

Language: Английский

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Diminished NAD+ levels and activation of retrotransposons promote postovulatory aged oocyte (POAO) death

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Feb. 28, 2024

Abstract Death is the fate of postovulatory aged or unfertilized oocytes (POAO) in many animals. However, precise molecular mechanisms are yet to be discovered. Here, we demonstrate that increased amounts reactive oxygen species (ROS), calcium ion (Ca+2) channels, and retrotransposon activity induce apoptosis, which turn causes POAO death. Notably, suppression ROS, Ca+2 retrotransposons delayed Further, found histone H4K12 K16 acetylation via downregulation NAD+ -dependent deacetylase SIRT3. Furthermore, adding NMN, sodium pyruvate, CD38 inhibition death oocytes. Finally, conservation retrotransposon-induced DNA damage-dependent higher-order vertebrates. Our findings suggest mortality caused by cyclic cascade metabolic interactions low levels increase inhibiting deacetylases, resulting an retrotransposons, channel thus contributing damage-induced apoptosis.

Language: Английский

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Arf6 GTPase deficiency leads to porcine oocyte quality decline during aging

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(13)

Published: June 17, 2024

Arf6 is a member of ADP-ribosylation factor (Arf) family, which widely implicated in the regulation multiple physiological processes including endocytic recycling, cytoskeletal organization, and membrane trafficking during mitosis. In this study, we investigated potential relationship between aging-related oocyte quality, its roles on organelle rearrangement cytoskeleton dynamics porcine oocytes. expressed oocytes throughout meiotic maturation, it decreased aged Disruption led to failure cumulus expansion polar body extrusion. Further analysis indicated that modulated ac-tubulin for spindle organization microtubule stability. Besides, regulated cofilin phosphorylation fascin actin assembly, further affected migration, indicating dynamics. Moreover, lack activity caused dysfunction Golgi ER protein synthesis signal transduction. Mitochondrial was also observed Arf6-deficient oocytes, supported by increased ROS level abnormal potential. conclusion, our results reported insufficient related aging-induced quality decline through

Language: Английский

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