New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple DOI Creative Commons

Diana J. McHenry,

Srđan G. Aćimović

Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 878 - 878

Published: April 27, 2024

Bitter rot of apple is an economically important worldwide disease caused by different Colletotrichum species, depending on many factors such as climate, geography, other hosts, and crop management practices. Culture, morphology, single-locus sequencing-based methods for identifying the species are severely limited in effectiveness, while multilocus sequence typing available delineating costly, time-intensive, require high expertise. We developed species-specific hydrolysis probe real-time PCR assays following nine causing bitter Mid-Atlantic U.S.A.: C. fructicola, chrysophilum, noveboracense, gloeosporioides s.s., henanense, siamense theobromicola from complex, fioriniae nymphaeae acutatum complex. After searching 14 gene regions, we designed primers probes 5 them target species. Four primer–probe set pairs were able to be duplexed. Sensitivity tests showed little 0.5 pg DNA detectable. These will provide rapid reliable identification these key critically studies aiming elucidate their biology, epidemiology, apples number one produced consumed tree fruit U.S.A.

Language: Английский

Development of a model for Colletotrichum diseases with calibration for phylogenetic clades on different host plants DOI Creative Commons
Irene Salotti, Yujie Liang, Tao Ji

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: March 29, 2023

Fungi in the genus

Language: Английский

Citations

11
Evolutionary history of the cytochrome P450s from Colletotrichum species and prediction of their putative functional roles during host-pathogen interactions DOI Creative Commons
Jossue Ortíz-Álvarez, Sioly Becerra, Riccardo Baroncelli

et al.

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: Jan. 12, 2024

The genomes of species belonging to the genus Colletotrichum harbor a substantial number cytochrome P450 monooxygenases (CYPs) encoded by broad diversity gene families. However, biological role their CYP complement (CYPome) has not been elucidated. Here, we investigated putative evolutionary scenarios that occurred during evolution CYPome Graminicola complex (s.c.) and implications. study revealed most families s.c. experienced contractions. reductive resulted in restricted CYPs are predominant each members from s.c., whereas only 18 absolutely conserved among these species. displayed notably different phylogenetic relationship at tertiary structure level, suggesting convergent scenario. Most enzymes share redundant functions secondary metabolite biosynthesis xenobiotic metabolism. Hence, this current work suggests presence plays critical optimization colonization capability virulence.

Language: Английский

Citations

4
Colletotrichum kahawae subsp. cigarro causing leaf spot disease on arecanut, Areca catechu L. in India: A first report DOI

R. Thava Prakasa Pandian,

Shivaji Hausrao Thube,

Bhavishya Bhavishya

et al.

Crop Protection, Journal Year: 2024, Volume and Issue: 179, P. 106607 - 106607

Published: Jan. 29, 2024

Language: Английский

Citations

4
MdWRKY71 promotes the susceptibility of apple to Glomerella leaf spot by controlling salicylic acid degradation DOI Creative Commons
Tingting Pei,

Dongshan Niu,

Yongxin Ma

et al.

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(4)

Published: April 1, 2024

Abstract Glomerella leaf spot (GLS), a fungal disease caused by Colletotrichum fructicola , severely affects apple ( Malus domestica ) quality and yield. In this study, we found that the transcription factor MdWRKY71 was significantly induced C. infection in GLS‐susceptible cultivar Royal Gala. The overexpression of leaves resulted increased susceptibility to whereas RNA interference showed opposite phenotypes. These findings suggest functions as for apple— interaction. Furthermore, directly bound promoter salicylic acid (SA) degradation gene Downy Mildew Resistant 6 DMR6 ‐Like Oxygenase 1 DLO1 promoted its expression, resulting reduced SA level. sensitivity 35S: can be effectively alleviated knocking down MdDLO1 confirming critical role MdWRKY71‐mediated via regulating expression GLS susceptibility. summary, identified factor, MdWRKY71, targets pathway promote infection.

Language: Английский

Citations

4
New Species-Specific Real-Time PCR Assays for Colletotrichum Species Causing Bitter Rot of Apple DOI Creative Commons

Diana J. McHenry,

Srđan G. Aćimović

Microorganisms, Journal Year: 2024, Volume and Issue: 12(5), P. 878 - 878

Published: April 27, 2024

Bitter rot of apple is an economically important worldwide disease caused by different Colletotrichum species, depending on many factors such as climate, geography, other hosts, and crop management practices. Culture, morphology, single-locus sequencing-based methods for identifying the species are severely limited in effectiveness, while multilocus sequence typing available delineating costly, time-intensive, require high expertise. We developed species-specific hydrolysis probe real-time PCR assays following nine causing bitter Mid-Atlantic U.S.A.: C. fructicola, chrysophilum, noveboracense, gloeosporioides s.s., henanense, siamense theobromicola from complex, fioriniae nymphaeae acutatum complex. After searching 14 gene regions, we designed primers probes 5 them target species. Four primer–probe set pairs were able to be duplexed. Sensitivity tests showed little 0.5 pg DNA detectable. These will provide rapid reliable identification these key critically studies aiming elucidate their biology, epidemiology, apples number one produced consumed tree fruit U.S.A.

Language: Английский

Citations

4