Exploring the Benefits of Probiotics in Gut Inflammation and Diarrhea—From an Antioxidant Perspective

Antioxidants, Journal Year: 2023, Volume and Issue: 12(7), P. 1342 - 1342

Published: June 26, 2023

Inflammatory bowel disease (IBD), characterized by an abnormal immune response, includes two distinct types: Crohn’s (CD) and ulcerative colitis (UC). Extensive research has revealed that the pathogeny of IBD encompasses genetic factors, environmental dysfunction, dysbiosis, lifestyle choices. Furthermore, patients with exhibit both local systemic oxidative damage caused excessive presence reactive oxygen species. This exacerbates response imbalances, intestinal mucosal damage, dysbiosis in patients. Meanwhile, weaning period represents a crucial phase for pigs, during which they experience pronounced inflammatory responses, leading to severe diarrhea increased mortality rates. Pigs are highly similar humans terms physiology anatomy, making them potential choice simulating human IBD. Although exact mechanism behind post-weaning remains unclear, its progression pathogenesis, is well acknowledged. Besides conventional anti-inflammatory drugs, certain probiotics, particularly Lactobacillus Bifidobacteria strains, have been found possess antioxidant properties. These include scavenging species, chelating metal ions inhibit Fenton reaction, regulation host enzymes. Consequently, numerous studies last decades committed exploring role probiotics alleviating Here, we sequentially discuss negative consequences stress on IBD, effectiveness treatment, application weaned piglets, mechanisms probiotics.

Language: Английский

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Effects of Probiotic-Fermented Feed on the Growth Profile, Immune Functions, and Intestinal Microbiota of Bamei Piglets

Animals, Journal Year: 2024, Volume and Issue: 14(4), P. 647 - 647

Published: Feb. 17, 2024

Purebred Bamei piglets present problems, including slow growth, respiratory disease, and post-weaning stress. This study investigated the effects of Lactobacillus plantarum QP28-1- Bacillus subtilis QB8-fermented feed supplementation on growth performance, immunity, intestinal microflora from Qinghai, China. A total 48 purebred (25 days; 6.8 ± 0.97 kg) were divided into following four groups for a 28-day diet experiment: basal (CK); containing 10% plantarum-fermented (L); subtilis-fermented (B); mixture 5% + (H). The daily weight gain food intake group H increased (p < 0.05), feed/weight ratios fed with fermented decreased more than that CK group. levels three immune factors, namely immunoglobulin (Ig)M, IgG, interferon-γ, higher whereas those tumor necrosis factor-α, interleukin (IL)-1β, IL-6 lower 0.05) in Total protein was while urea nitrogen, cholesterol triglycerides mixed-fermented Analysis gut microbiota showed addition α-diversity microbiota, increasing abundances probiotics Lactobacillus, Muribaculaceae, Ruminococcaceae, Prevotellaceae, Rikenellaceae. Additionally, correlation analysis demonstrated several these probiotic bacteria closely related to serum immunity. In conclusion, rebuilt piglets, thereby reducing ratio, improving intake, enhancing

Language: Английский

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Bacillus halotolerans SW207 alleviates enterotoxigenic Escherichia coli -induced inflammatory responses in weaned piglets by modulating the intestinal epithelial barrier, the TLR4/MyD88/NF-κB pathway, and intestinal microbiota

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(4)

Published: March 7, 2024

Enterotoxigenic Escherichia coli (ETEC) is one of the major pathogens contributing to piglet diarrhea, with significant implications for both health and economic aspects livestock industry. SW207 an isolate Bacillus halotolerans isolated from cold- disease-resistant Leixiang pigs in Northeastern China. We have discovered that can survive pig's gastrointestinal fluid under conditions high bile salt concentration, displaying potent antagonistic activity against ETEC. In this study, we established a weaned diarrhea model infected ETEC investigate role preventing improving intestinal health. Results indicate upregulates expression tight junction proteins, including claudin-1, occludin, zonula occludens-1, at transcriptional translational levels. Furthermore, reduces serum endotoxin, D-lactic acid, various oxidative stress markers while enhancing mechanical barrier function. terms immune barrier, suppressed activation TLR4/MyD88/NF-κB pathway, reducing inflammatory factors upregulating small intestine mucosal sIgA. Concerning biological significantly content E. intestines promotes abundance beneficial bacteria, thereby mitigating microbiota imbalance caused by summary, has potential prevent ETEC, alleviate inflammation epithelial damage, facilitate changes microbiota. This contributes elucidating mechanisms host-microbe interactions pathogen infections.IMPORTANCEEnterotoxigenic consistently been causing mortality piglets pig farming. The industry traditionally relied on antibiotic administration control ETEC-induced diarrhea. However, overuse antibiotics led emergence drug-resistant zoonotic bacterial pathogens, posing threat public Therefore, there urgent need identify alternatives reduce usage. assessed protective effect novel probiotic analyzed its vivo vitro. study results provide theoretical support reference implementing interventions gut early improve

Language: Английский

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The Effect of an Essential Oil Blend on Growth Performance, Intestinal Health, and Microbiota in Early-Weaned Piglets

Nutrients, Journal Year: 2023, Volume and Issue: 15(2), P. 450 - 450

Published: Jan. 14, 2023

Essential oils (EO) are promising feed additives for their antibacterial, antioxidant, and immune-enhancing abilities with low toxicity. Carvacrol, thymol, cinnamaldehyde commonly used to synthesize EO. However, few studies focus on combining these three EO in early-weaned piglets. In the present study, 24 piglets weaned at 21 d of age were randomly divided into 2 groups (6 replicate pens per group, pen). The fed a basal diet (the control group) supplemented 400 mg/kg (a blend consisting carvacrol, cinnamaldehyde, 28 days. At end experiment, one piglet pen was chosen be sacrificed. Growth performance, hematology, plasma biochemical indices, antioxidant capacity, intestinal epithelial development immunity, colonic volatile fatty acids (VFA), microbiota determined. results indicated that significantly improved average daily intake (ADFI, p < 0.01) gain (ADG, 0.05) day 0 period. supplementation led significant decrease lysozyme (p cortisol levels 0.01). Additionally, promoted jejunal goblet cells villus, mucosa ZO-1 mRNA expression, ileal villus height, height/crypt depth ratio 0.05). mucosal TLR4 NFκB p-p65/p65 protein expression inhibited group Colonic digesta analysis revealed bacteria involving Erysipelotrichaceae family, Holdemanella genus, Phascolarctobacterium Vibrio genus enriched group. conclusion, findings indicate improves ADG ADFI period, as well immunity piglets, which provides theoretical basis combined use

Language: Английский

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Bacillus amyloliquefaciens SC06 alleviated intestinal damage induced by inflammatory via modulating intestinal microbiota and intestinal stem cell proliferation and differentiation

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 130, P. 111675 - 111675

Published: Feb. 19, 2024

Language: Английский

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Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea

Current Research in Microbial Sciences, Journal Year: 2025, Volume and Issue: 8, P. 100349 - 100349

Published: Jan. 1, 2025

Blastocystis sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on distribution its circulating subtypes (STs) from the terrestrial environment are available, while those marine remain still scare. A 16S rRNA gene sequencing analysis were conducted over period 2022-2024 by screening fecal samples four different species of free-ranging mammals (sperm, fin, long-finned pilot Cuvier's beaked whales) within North-Western Mediterranean Sea. 10 out 43 (23.2 %) found positive to using molecular tools. predominance zoonotic subtype ST3 among as well presence ST1 allele 4 even untypable fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes Proteobacteria Blastocystis-carrier mammal identification Archaebacteria Methanomethylophilaceae family isolate detected Illumina V3-V4 generated data. The present survey presents new insights regarding prevalence ST1-ST3 environment, associated gut microbiome, providing hence baseline for better understanding risk prevent human ecosystem exposure these anthropogenic microorganisms.

Language: Английский

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Integrated multi-omics reveals the Bacillus amyloliquefaciens BA40 against Clostridium perfringens infection in weaned piglets

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Clostridium perfringens (C. perfringens) can cause necrotic enteritis and higher mortality rates in piglets, by impairing the intestinal barrier function. Bacillus amyloliquefaciens 40 (BA40) has showed potential ability to reduce C. infections, but mechanisms responsible for its effectiveness remain unclear. This study aims evaluate impact of BA40 on inflammation induced explain underlying therapeutic effects. We aim show how bolster piglet health strengthening regulating immune responses. used piglets cellular models, alongside microbiomics, metabolomic, transcriptomic analyses, investigate BA40's perfringens-induced inflammation. A model infection was constructed using cells effect phenotype. Microbiomics, metabolomics, transcriptomics analyses were subsequently protection response piglets. Our revealed significant improvements following administration. Notably, strengthened mucosal mitigated inflammatory triggered decreased harmful bacteria increased beneficial bacteria. Metabolite profiles improved, showing a reduction substances. Transscriptomics analysis indicated role TNF/NF-κB signaling pathway, hinting at regulate responses Cellular assays further confirmed capacity diminish cytokine release encourage differentiation anti-inflammatory macrophages. Datasets from present demonstrate that modulates gut microbes metabolites, inhibits inflammation-related pathways, maintains findings not only deepen our understanding also provide theoretical foundation development probiotics alternative therapies aimed improving health.

Language: Английский

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The Effect of an Early-Life Lactiplantibacillus plantarum LPJZ-658 Intervention on Performance and Gut Microbiota in Suckling Piglets

Microbiology Research, Journal Year: 2025, Volume and Issue: 16(2), P. 41 - 41

Published: Feb. 7, 2025

This study aimed to investigate the effects of early-life supplementation with Lactiplantibacillus plantarum LPJZ-658 on growth performance and gut microbiota newborn piglets. Twelve one-day-old suckling piglets were randomly divided into two groups: control group (C) was orally administered saline (LP) gavaged 1.0 × 1010 cfu LPJZ-658. The carried out once daily for 28 days. Fresh fecal samples collected at 7 days, respectively. composition (16S rRNA gene amplicon sequencing) its predicted functions (PICRUSt2) analyzed. body weight average gain significantly increased in LP group. Statistically significant differences observed bacterial diversity microbial community between C groups. predominant phylum changed from Firmicutes, Bacteroidetes, Proteobacteria day Spirochaetota both We found that suppressed a decrease relative abundance Bacteroidota At genus level, reduced pathogenic genera such as Clostridium sensu stricto 1, beneficial Ruminococcus, Christensenellaceae_R-7_group, Turicibacter, UCG-002. KEGG metabolic pathway analysis showed may improve amino acid metabolism by regulating intestinal In summary, interventions modulation microbiota.

Language: Английский

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Comprehensive Phenotypic Characterization and Genomic Analysis Unveil the Probiotic Potential of Bacillus velezensis K12

Animals, Journal Year: 2025, Volume and Issue: 15(6), P. 798 - 798

Published: March 11, 2025

Bacillus spp. have emerged as pivotal sources of probiotic preparations, garnering considerable attention in recent years owing to their vigorous bacteriostatic activity and antimicrobial resistance. This study aimed investigate these characteristics depth verify the safety velezensis K12, a strain isolated from broiler intestine. The K12 was identified based on its morphology 16S rDNA sequence homology analysis. Subsequently, B. evaluated for acid resistance, bile salt gastrointestinal tolerance, drug sensitivity, activity. Additionally, whole-genome sequencing technology employed dissect genomic components further, aiming explore potential applications strain. sensitive six antibiotics had tolerance. Furthermore, it showed potent against wide range pathogenic bacteria, including Escherichia coli (E. coli), Staphylococcus aureus, Salmonella, Clostridium perfringens, cereus, Vibrio parahaemolyticus. complete genome revealed length 3,973,105 base pairs containing 4123 coding genes, among which 3973 genes were functionally annotated. analysis associated with adhesion, antioxidants, secondary metabolite production, whereas no functional related enterotoxins or transferable antibiotic resistance detected, thereby confirming properties K12. exhibits broad-spectrum vitro safety, positioning candidate developing preparations.

Language: Английский

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Effects of Saccharomyces boulardii cell wall polysaccharide on growth performance, immunity, antioxidant ability and ileal mucosal morphology of Jinhua weaned piglets in Northern Xinjiang region

Frontiers in Animal Science, Journal Year: 2025, Volume and Issue: 6

Published: March 17, 2025

Background The high rates of diarrhea, mortality, and feed conversion ratio (F/G) among weaned piglets have significantly hindered the development pig feeding practices in Jinhua, located North Xinjiang region. Saccharomyces boulardii cell wall polysaccharide (SBWP) is a probiotic that has potential to enhance gastrointestinal immunity health, while also reducing systemic inflammation, diarrhea rates, mortality. This study investigates effects SBWP on performance, immune capacity, antioxidant mucosal morphology from region, through 28-day trial. Methods A total 64 male Jinhua piglets, aged 28 days with mean body weight 4.23 ± 0.53 kg, were utilized this study. randomly assigned four experimental groups, each consisting pens per pen, based varying percentages as supplement. supplementation concentrations for groups 0% (control group), 0.2%, 0.5%, 1.0% SBWP, respectively. Results Compared CON group, 0.5% demonstrated increase average daily intake (ADFI) gain (ADG), alongside decrease feed-to-gain (F/G). Additionally, fecal score frequency reduced, no mortality recorded these which represented 37.50% reduction compared group. In terms ileal immunity, reduced pro-inflammatory cytokines, including interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), well diamine oxidase (DAO). Conversely, both exhibited elevated anti-inflammatory cytokine interleukin-10 (IL-10). Furthermore, addition increased secretory immunoglobulin (SIgA). serum had TNF-α, IL-1β, IL-6 DAO. Regarding capacity mucosa, content malondialdehyde (MDA) hydrogen peroxide (H 2 O ) decreased, catalase (CAT), glutathione peroxidase (GSH-Px), (T-AOC) increased, superoxide dismutase (SOD) activity was enhanced. Lastly, basal diet resulted promotion villus height crypt depth (VCR), thereby improving mucosa. conclusion, growth performance northern improved by 0.5-1.0% when supplemented diet. enhancement attributed function, enhanced morphology, inflammatory factors mucosa serum.

Language: Английский

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