Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
12(4)
Published: Feb. 20, 2024
ABSTRACT
Given
the
burgeoning
Nyctereutes
procyonoides
breeding
industry
and
its
growing
scale,
it
is
imperative
to
investigate
impact
of
high-fat
diets
on
health
these
animals.
This
study
involved
30
male
comparable
weights
(3
kg
±0.5),
randomly
assigned
either
a
control
group
or
diet
(
n
=
15
each).
The
latter
was
fed
mixture
lard
basal
in
2:5
ratio,
establishing
model
procyonoides.
induced
diarrhea
histopathological
changes
Analysis
small
intestine
contents
using
16S
rRNA
sequencing
revealed
diet-induced
disruption
gut
microbiota.
Specifically,
Escherichia-Shigella
emerged
as
biomarker
P
0.049),
while
Vagococcus
prevalent
indicating
significant
increase
harmful
bacteria
group.
Furthermore,
this
disrupted
flora
correlated
with
inflammation
oxidative
stress,
evidenced
by
marked
increases
TNF-α
<
0.01),
IL-1β
0.05),
IL-6
0.05)
levels,
measured
via
q-PCR,
Western
blot,
stress
assays.
In
addition,
q-PCR
analysis
upregulation
apoptosis
necrosis
markers,
including
Bax,
Caspase3,
Caspase9,
Caspase12,
RIPK3,
RIPK1
0.01
0.001),
concurrent
downregulation
anti-apoptotic
gene
Bcl-2
0.01)
group,
consistent
protein
expression
trends.
These
findings
suggest
that
alters
microbiome
toward
more
bacterial
composition,
escalating
inflammatory
responses
intestinal
tissue
permeability,
culminating
cell
necrosis.
IMPORTANCE
examines
Our
research
established
diet,
revealing
impacts,
such
diarrhea,
histological
anomalies,
alterations
emphasize
importance
preventing
issues
promoting
sustainable
growth.
They
highlight
microbiota
overall
animal
health.
Ecotoxicology and Environmental Safety,
Journal Year:
2024,
Volume and Issue:
278, P. 116415 - 116415
Published: May 3, 2024
The
combined
pollution
of
microplastics
(MPs)
and
sulfamethoxazole
(SMZ)
often
occurs
in
aquatic
ecosystems,
posing
a
serious
threat
to
animal
human
health.
However,
little
is
known
about
the
liver
damage
caused
by
single
or
co-exposure
MPs
SMZ,
its
specific
mechanisms
are
still
poorly
understood.
In
this
study,
we
investigated
effects
20
μm
80
nm
SMZ
both
larval
adult
zebrafish
models.
Firstly,
observed
significant
decrease
number
hepatocytes
worsened
following
MPs.
Additionally,
macrophages
neutrophils
decreased,
while
expression
inflammatory
cytokines
antioxidant
enzyme
activities
increased
after
zebrafish.
Transcriptome
analysis
revealed
changes
gene
co-exposed
groups,
particularly
processes
related
oxidation-reduction,
response,
MAPK
signaling
pathway
Co-exposure
also
promoted
hepatocyte
apoptosis
inhibited
proliferation
levels,
which
was
associated
with
translocation
Nrf2
from
cytoplasm
nucleus
an
increase
protein
levels
NF-kB
p65
Furthermore,
our
pharmacological
experiments
demonstrated
that
inhibiting
ROS
blocking
partially
rescued
injury
induced
conclusion,
findings
suggest
induces
hepatic
dysfunction
through
ROS-mediated
This
information
provides
novel
insights
into
potential
environmental
risk
hazardous
pollutants
co-existence
ecosystems.
Nano Letters,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 29, 2025
To
explore
the
intergenerational
cardiotoxicity
of
nanoplastics,
maternal
mice
were
exposed
to
60
nm
polystyrene
nanoplastics
(PS-NP)
during
pregnancy
and
lactation.
The
results
showed
that
PS-NP
can
enter
hearts
offspring
induce
myocardial
fiber
arrangement
disorder,
acidophilic
degeneration
cardiomyocytes,
elevated
creatine
kinase
isoenzymes
(CK-MB)
lactate
dehydrogenase
(LDH)
levels
after
exposure
at
100
mg/kg
Mechanistically,
KEGG
analysis
RNA
sequencing
participation
hypoxia-inducible
factor-1
(HIF-1)
ferroptosis
in
PS-NP-induced
cardiotoxicity.
Key
features
ferroptosis,
including
Fe2+
accumulation,
mitochondrial
injury,
oxidative
stress,
GPX4
downregulation,
FTH1,
ACSL4,
SLC7A11
upregulation,
detected.
Furthermore,
treatment
upregulated
expressions
HIF-1α
HO-1,
be
alleviated
by
inhibition
using
si-HIF-1α.
This
study
provided
an
insightful
reference
for
assessment
PS-NP.
Food & Function,
Journal Year:
2024,
Volume and Issue:
15(7), P. 3411 - 3419
Published: Jan. 1, 2024
Tetrabromobisphenol
A
(TBBPA)
is
a
global
pollutant.
When
TBBPA
absorbed
by
the
body
through
various
routes,
it
can
have
wide
range
of
harmful
effects
on
body.
Green
tea
polyphenols
(GTPs)
act
as
antioxidants,
resisting
toxic
animals.
The
and
mechanisms
GTP
oxidative
stress,
inflammation
apoptosis
in
mouse
lung
are
unknown.
Therefore,
we
established
vivo
vitro
models
exposure
antagonism
using
C57
mice
A549
cells
examined
expression
factors
related
to
autophagy,
apoptosis.
results
study
showed
that
increase
reactive
oxygen
species
(ROS)
levels
after
decreased
autophagy-related
Beclin1,
LC3-II,
ATG3,
ATG5,
ATG7
ATG12
increased
p62;
stress
inhibits
autophagy
levels.
pro-inflammatory
IL-1β,
IL-6
TNF-α
anti-inflammatory
factor
IL-10
activation
NF-κB
p65/TNF-α
pathway.
Bax,
caspase-3,
caspase-7
caspase-9
Bcl-2
activate
apoptosis-related
pathways.
addition
attenuated
levels,
restored
inhibition
reduced
Our
suggest
attenuate
modulating
ROS,
reducing
increasing
attenuating
cells.
These
provide
fundamental
information
for
exploring
antioxidant
mechanism
further
studying
TBBPA.
Poultry Science,
Journal Year:
2024,
Volume and Issue:
103(6), P. 103674 - 103674
Published: March 16, 2024
Microplastics
biological
toxicity,
environmental
persistence
and
chemicals
have
been
paid
widespread
attention.
exposed
to
chicken
spleen
injury
of
the
specific
mechanism
is
unclear.
Thus,
we
randomly
assigned
chickens
4
groups:
C
(normal
diet),
L-MPs
(1
mg/L),
M-MPs
(10
H-MPs
(100
assessed
damage
after
42
d
exposure.
Morphologically,
boundary
between
red
white
pulp
was
blurred,
along
with
expansion
pulp.
It
further
speculated
that
microplastics
induced
mitochondrial
dynamic
homeostasis
(Drp1
upgraded,
Mfn1,
Mfn2,
OPA1
reduced),
provoked
apoptotic
pathway
(Bcl-2/Bax
decreased,
cytc,
caspase3,
caspase9
raised),
resulting
in
redox
imbalance
lipid
peroxide
accumulation
(MDA
increased,
CAT,
GSH,
T-AOC
plummeted),
stimulated
ferroptosis
(FTH1,
GPX4,
SLC7A11
decreased).
Here
explored
impact
polystyrene
on
spleen,
as
well
programmed
death
(apoptosis
ferroptosis)
involved,
regulative
role
mitochondria
this
process.
This
could
be
significant
importance
bridging
gap
laboratory
research
microplastics-induced
chicken.
