Organ-on-a-chip with integrated semitransparent organic electrodes for barrier function monitoring DOI Creative Commons
Denise Marrero, Anton Guimerà‐Brunet, Laure Maes

et al.

Lab on a Chip, Journal Year: 2023, Volume and Issue: 23(7), P. 1825 - 1834

Published: Jan. 1, 2023

Organs-on-a-chip (OoC) are cell culture platforms that replicate key functional units of tissues in vitro. Barrier integrity and permeability evaluation utmost importance when studying barrier-forming tissues. Impedance spectroscopy is a powerful tool widely used to monitor barrier real-time. However, data comparison across devices misleading due the generation non-homogenous field tissue barrier, making impedance normalization very challenging. In this work, we address issue by integrating PEDOT:PSS electrodes for function monitoring with spectroscopy. The semitransparent cover entire membrane providing homogenous electric area equally accountable measured impedance. To best our knowledge, has never been solely cellular barriers while enabling optical inspection OoC. performance device demonstrated lining intestinal cells where monitored formation under flow conditions, as well disruption recovery exposure enhancer. tightness integrity, intercellular cleft have evaluated analyzing full spectrum. Furthermore, autoclavable paving way toward more sustainable OoC options.

Language: Английский

In vitro models replicating the human intestinal epithelium for absorption and metabolism studies: A systematic review DOI Creative Commons
Arianna Fedi, Chiara Vitale,

Giulia Ponschin

et al.

Journal of Controlled Release, Journal Year: 2021, Volume and Issue: 335, P. 247 - 268

Published: May 24, 2021

Absorption, distribution, metabolism and excretion (ADME) studies represent a fundamental step in the early stages of drug discovery. In particular, absorption orally administered drugs, which occurs at intestinal level, has gained attention since poor oral bioavailability often led to failures for new approval. this context, several vitro preclinical models have been recently developed optimized better resemble human physiology lab serve as an animal alternative accomplish 3Rs principles. However, numerous are ineffective recapitulating key features small intestine epithelium lack prediction potential during stage. review, we provide overview aimed mimicking barrier pharmaceutical screening. After briefly describing how works, present i) conventional 2D synthetic cell-based systems, ii) 3D replicating main architecture, iii) micro-physiological systems (MPSs) reproducing dynamic stimuli cells exposed native microenvironment. will highlight benefits drawbacks leading used studies.

Language: Английский

Citations

149
Advances in Engineering Human Tissue Models DOI Creative Commons
Chrysanthi‐Maria Moysidou, Chiara Barberio, Róisı́n M. Owens

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2021, Volume and Issue: 8

Published: Jan. 28, 2021

Research in cell biology greatly relies on cell-based

Language: Английский

Citations

120
Response of cells and tissues to shear stress DOI Creative Commons
Jaime A. Espina, Marília H. Cordeiro, Milan Milivojević

et al.

Journal of Cell Science, Journal Year: 2023, Volume and Issue: 136(18)

Published: Sept. 15, 2023

ABSTRACT Shear stress is essential for normal physiology and malignancy. Common physiological processes – such as blood flow, particle flow in the gut, or contact between migratory cell clusters their substrate produce shear that can have an impact on behavior of different tissues. In addition, has roles biomedical interest, wound healing, cancer fibrosis induced by soft implants. Thus, understanding how cells react adapt to important. this Review, we discuss vivo vitro data obtained from vascular epithelial models; highlight insights these afforded regarding general mechanisms through which sense, transduce respond at cellular levels; outline changes experience response tissue organization. Finally, role collective migration, only starting be appreciated. We review our current effects context embryo development, fibrosis, invite scientific community further investigate scenarios.

Language: Английский

Citations

52
Dissecting Gut‐Microbial Community Interactions using a Gut Microbiome‐on‐a‐Chip DOI Creative Commons
Jeeyeon Lee, Nishanth Venugopal Menon, Chwee Teck Lim

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(20)

Published: Feb. 27, 2024

Abstract While the human gut microbiota has a significant impact on health and disease, understanding of roles microbes, interactions, collective microbes various aspects is limited by lack suitable in vitro model system that can accurately replicate gut‐like environment enable close visualization causal mechanistic relationships between microbial constitutents gut. , In this study, we present scalable Gut Microbiome‐on‐a‐Chip (GMoC) with great imaging capability scalability, providing physiologically relevant dynamic gut‐microbes interfaces. This chip features reproducible 3D stratified epithelium derived from Caco‐2 cells (µGut), mimicking key intestinal architecture, functions, cellular complexity, physiolocially for residing Incorporating tumorigenic bacteria, enterotoxigenic Bacteroides fragilis (ETBF), into GMoC observation pathogenic behaviors ETBF, leading to µGut disruption pro‐tumorigenic signaling activations. Pre‐treating beneficial microbe Lactobacillus spp., effectively prevent ETBF‐mediated pathogenesis, preserving healthy state through competition‐mediated colonization resistance. The holds potential as valuable tool exploring unknown microbe‐induced pathogenesis microbe‐based therapeutic development.

Language: Английский

Citations

18
Gut-on-a-chip: Mimicking and monitoring the human intestine DOI Creative Commons
Denise Marrero, Ferran Pujol‐Vila, Daniel Vera

et al.

Biosensors and Bioelectronics, Journal Year: 2021, Volume and Issue: 181, P. 113156 - 113156

Published: March 9, 2021

Over the last few years, intestine has been extensively studied using in vitro microfluidic systems, commonly known as gut-on-a-chip (GOC) devices. This interest due not only to importance of intestine's proper functions but also relationship that this organ and microbiota inhabits it with rest body's organs. The increased complexity these together need improve our understanding intestinal physiology interdependencies, led greater focus on integration biosensors within However, current number GOC devices integrated sensors for monitoring relevant physiological parameters are very limited demand use external analytical techniques delay analysis prevent real-time decision-making. paper reviews various materials, technologies, structures have used both mimicking parameters, such permeability gut barrier, dissolved oxygen concentration, cytokines profile production microbial short-chain fatty acids. We propose alternative biosensing demonstrated other lab-on-a-chip could be translated models. A critical requirements, limitations, challenges microenvironment replication monitorization models is included, a particular biomarkers should detected simultaneously get framework function until now, received necessary attention.

Language: Английский

Citations

98
Developing an advanced gut on chip model enabling the study of epithelial cell/fibroblast interactions DOI Creative Commons

Marine Verhulsel,

Anthony Simon,

Moencopi Bernheim-Dennery

et al.

Lab on a Chip, Journal Year: 2020, Volume and Issue: 21(2), P. 365 - 377

Published: Dec. 11, 2020

We report on a new gut chip combining the co-culture of primary epithelial and stromal cells in 3D biomimetic scaffold. Proper segregation dividing differentiated along crypt-villus axis was achieved these unique conditions.

Language: Английский

Citations

71
Pulmonary delivery nanomedicines towards circumventing physiological barriers: Strategies and characterization approaches DOI
Wenhao Wang, Zhengwei Huang, Ying Huang

et al.

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 185, P. 114309 - 114309

Published: April 22, 2022

Language: Английский

Citations

57
Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro DOI Creative Commons
Perizat Kanabekova,

Adina Kadyrova,

Gulsim Kulsharova

et al.

Micromachines, Journal Year: 2022, Volume and Issue: 13(3), P. 428 - 428

Published: March 10, 2022

Mortality from liver disease conditions continues to be very high. As diseases manifest and progress silently, prompt measures after diagnosis are essential in the treatment of these conditions. Microfluidic organs-on-chip platforms have significant potential for study pathophysiology vitro. Different liver-on-a-chip microphysiological been reported cell-signaling pathways such as those activating stellate cells within diseases. Moreover, drug efficacy might evaluated on a cellular metabolic level. Here, we present comprehensive review used modelling First, briefly introduce concept importance organs-on-a-chip studying vitro, reflecting existing reviews healthy platforms. Second, techniques cell cultures microfluidic devices, including 2D, 3D, spheroid cells, explained. Next, types (NAFLD, ALD, hepatitis infections, injury) on-chip explained further overview design methods developing Finally, some challenges solutions them reviewed.

Language: Английский

Citations

45
Organ-on-a-chip: A new tool for in vitro research DOI
Jiasheng Yan, Ziwei Li, Jiuchuan Guo

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 216, P. 114626 - 114626

Published: Aug. 10, 2022

Language: Английский

Citations

43
Integrated-gut-liver-on-a-chip platform as an in vitro human model of non-alcoholic fatty liver disease DOI Creative Commons
Jiandong Yang, Yoshikazu Hirai, Kei Iida

et al.

Communications Biology, Journal Year: 2023, Volume and Issue: 6(1)

Published: March 23, 2023

Non-alcoholic fatty liver disease (NAFLD) afflicts a significant percentage of the population; however, no effective treatments have yet been established because unsuitability in vitro assays and animal experimental models. Here, we present an integrated-gut-liver-on-a-chip (iGLC) platform as human model gut-liver axis (GLA) by co-culturing gut cell lines interconnected via microfluidics closed circulation loop, for initiation progression NAFLD treatment with free acids (FFAs) 1 7 days, respectively. Co-cultured Caco-2 gut-mimicking cells HepG2 hepatocyte-like demonstrate protective effects from apoptosis against FFAs treatment, whereas mono-cultured exhibit induced apoptosis. Phenotype gene expression analyses reveal that FFAs-treated accumulated intracellular lipid droplets show increase associated cellular response to copper ions endoplasmic reticulum stress. As GLA model, iGLC may serve alternative experiments investigating mechanisms NAFLD.

Language: Английский

Citations

42