Nitric Oxide: Physiological Functions, Delivery, and Biomedical Applications

Advanced Science, Journal Year: 2023, Volume and Issue: 10(30)

Published: Aug. 26, 2023

Nitric oxide (NO) is a gaseous molecule that has central role in signaling pathways involved numerous physiological processes (e.g., vasodilation, neurotransmission, inflammation, apoptosis, and tumor growth). Due to its form, NO short half-life, physiology concentration dependent, often restricting function target site. Providing from an external source beneficial promoting cellular functions treatment of different pathological conditions. Hence, the multifaceted pathology garnered massive interest developing strategies deliver exogenous for various regenerative biomedical complexities. NO-releasing platforms or donors capable delivering controlled sustained manner tissues organs have advanced past few decades. This review article discusses detail generation via enzymatic synthase as well multiple biological modulates. The methods incorporating into diverse biomaterials including physical, chemical, supramolecular techniques are summarized. Then, these highlighted terms advancing medical problems.

Language: Английский

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Microenvironment responsive nanocomposite hydrogel with NIR photothermal therapy, vascularization and anti-inflammation for diabetic infected wound healing

Bioactive Materials, Journal Year: 2023, Volume and Issue: 26, P. 306 - 320

Published: March 14, 2023

Bacterial infection, excessive inflammation and damaging blood vessels network are the major factors to delay healing of diabetic ulcer. At present, most wound repair materials passive can't response microenvironment, resulting in a low utilization bioactive substances hence poor therapeutic effect. Therefore, it's essential design an intelligent dressing responsive microenvironment achieve release drugs on-demand on basis multifunctionality. In this work, metformin-laden CuPDA NPs composite hydrogel (Met@ NPs/HG) was fabricated by dynamic phenylborate bonding gelatin modified dopamine (Gel-DA), Cu-loaded polydopamine nanoparticles (CuPDA NPs) with hyaluronic acid phenyl boronate (HA-PBA), which possessed good injectability, self-healing, adhesive DPPH scavenging performance. The slow metformin achieved interaction NPs, boric groups (B-N coordination) constraint network. Metformin had pH glucose behavior treat different intelligently. Moreover, endowed excellent photothermal responsiveness kill bacteria >95% within 10 min also Cu2+ protect from infection for long time. Met@ NPs/HG recruited cells certain direction promoted vascularization releasing Cu2+. More importantly, Met@CuPDA effectively decreased eliminating ROS inhibiting activation NF-κB pathway. Animal experiments demonstrated that significantly SD rats killing bacteria, inflammation, improving angiogenesis accelerating deposition ECM collagen. great application potential healing.

Language: Английский

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Multifunctional Hydrogels for the Healing of Diabetic Wounds

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 13, 2023

Abstract The healing of diabetic wounds is hindered by various factors, including bacterial infection, macrophage dysfunction, excess proinflammatory cytokines, high levels reactive oxygen species, and sustained hypoxia. These factors collectively impede cellular behaviors the process. Consequently, this review presents intelligent hydrogels equipped with multifunctional capacities, which enable them to dynamically respond microenvironment accelerate wound in ways, stimuli ‐responsiveness, injectable self‐healing, shape ‐memory, conductive real‐time monitoring properties. relationship between multiple functions also discussed. Based on wounds, antibacterial, anti‐inflammatory, immunomodulatory, antioxidant, pro‐angiogenic strategies are combined hydrogels. application repair systematically discussed, aiming provide guidelines for fabricating exploring role therapeutic processes.

Language: Английский

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Reactive Oxygen Species‐Scavenging Nanosystems in the Treatment of Diabetic Wounds

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(25)

Published: April 13, 2023

Diabetic wounds are characterized by drug-resistant bacterial infections, biofilm formation, impaired angiogenesis and perfusion, oxidative damage to the microenvironment. Given their complex nature, diabetic remain a major challenge in clinical practice. Reactive oxygen species (ROS), which have been shown trigger hyperinflammation excessive cellular apoptosis, play pivotal role pathogenesis of wounds. ROS-scavenging nanosystems recently emerged as smart multifunctional nanomedicines with broad synergistic applicability. The documented anti-inflammatory pro-angiogenic ability treatments predestines these promising options for treatment Yet, this context, therapeutic applicability efficacy be elucidated. Herein, ROS is deciphered, properties strengths capacity summarized. In addition, current challenges such potential future directions discussed through clinical-translational lens.

Language: Английский

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Multifunctional PtCuTe Nanosheets with Strong ROS Scavenging and ROS‐Independent Antibacterial Properties Promote Diabetic Wound Healing

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(8)

Published: Sept. 19, 2023

Abstract Nanozymes, as one of the most efficient reactive oxygen species (ROS)‐scavenging biomaterials, are receiving wide attention in promoting diabetic wound healing. Despite recent attempts at improving catalytic efficiency Pt‐based nanozymes (e.g., PtCu, best systems), they still display quite limited ROS scavenging capacity and ROS‐dependent antibacterial effects on bacteria or immunocytes, which leads to uncontrolled poor Hence, a new class multifunctional PtCuTe nanosheets with excellent catalytic, ROS‐independent antibacterial, proangiogenic, anti‐inflammatory, immuno‐modulatory properties for boosting healing, is reported. The show stronger better than PtCu. It also revealed that can enhance vascular tube formation, stimulate macrophage polarization toward M2 phenotype improve fibroblast mobility, outperforming conventional Moreover, promotes crosstalk between different cell types form positive feedback loop. Consequently, stimulates proregenerative environment relevant populations ensure normal tissue repair. Utilizing mouse model, it demonstrated significantly facilitated regeneration highly vascularized skin, percentage closure being over 90% 8th day, among reported comparable biomaterials.

Language: Английский

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Mild Photothermal‐Stimulation Based on Injectable and Photocurable Hydrogels Orchestrates Immunomodulation and Osteogenesis for High‐Performance Bone Regeneration

Small, Journal Year: 2023, Volume and Issue: 19(28)

Published: May 16, 2023

Abstract A photoactivated bone scaffold integrated with minimally invasive implantation and mild thermal‐stimulation capability shows great promise in the repair regeneration of irregularly damaged tissues. Developing multifunctional photothermal biomaterials that can simultaneously serve as both controllable thermal stimulators biodegradable engineering scaffolds for immunomodulation, infection therapy, impaired remains an enormous challenge. Herein, injectable photocurable hydrogel therapeutic platform (AMAD/MP) based on alginate methacrylate, alginate‐graft‐dopamine, polydopamine (PDA)‐functionalized Ti3C2 MXene (MXene@PDA) nanosheets is rationally designed near‐infrared (NIR)‐mediated synergistic osteogenesis, bacterial elimination. The optimized AMAD/MP exhibits favorable biocompatibility, osteogenic activity, immunomodulatory functions vitro. proper immune microenvironment provided by could further modulate balance M1/M2 phenotypes macrophages, thereby suppressing reactive oxygen species‐induced inflammatory status. Significantly, this stimulation efficiently attenuates local reactions promotes new formation without addition exogenous cells, cytokines, or growth factors. This work highlights potential application advanced providing on‐demand cues tissue regenerative medicine.

Language: Английский

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A tough, antibacterial and antioxidant hydrogel dressing accelerates wound healing and suppresses hypertrophic scar formation in infected wounds

Bioactive Materials, Journal Year: 2024, Volume and Issue: 34, P. 269 - 281

Published: Jan. 1, 2024

Wound management is an important issue that places enormous pressure on the physical and mental health of patients, especially in cases infection, where increased inflammatory response could lead to severe hypertrophic scars (HSs). In this study, a hydrogel dressing was developed by combining high strength toughness, swelling resistance, antibacterial antioxidant capabilities. The matrix composed double network polyvinyl alcohol (PVA) agarose with excellent mechanical properties. Hyperbranched polylysine (HBPL), highly effective cationic polymer, tannic acid (TA), strong molecule, were added as functional components. Examination properties confirmed full play efficacy HBPL TA. vivo studies methicillin-resistant Staphylococcus aureus (MRSA) had shown obvious promotion wound healing, more profoundly, significant suppression scar formation. Due common raw materials simple preparation methods, can be mass produced used for accelerating healing while preventing HSs infected wounds.

Language: Английский

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3D‐Printed Functional Hydrogel by DNA‐Induced Biomineralization for Accelerated Diabetic Wound Healing

Advanced Science, Journal Year: 2023, Volume and Issue: 10(17)

Published: April 19, 2023

Chronic wounds in diabetic patients are challenging because their prolonged inflammation makes healing difficult, thus burdening patients, society, and health care systems. Customized dressing materials needed to effectively treat such that vary shape depth. The continuous development of 3D-printing technology along with artificial intelligence has increased the precision, versatility, compatibility various materials, providing considerable potential meet abovementioned needs. Herein, functional inks comprising DNA from salmon sperm DNA-induced biosilica inspired by marine sponges, developed for machine learning-based wound dressings. biomineralized silica incorporated into hydrogel a fast, facile manner. 3D-printed generates provided appropriate porosity, characterized effective exudate blood absorption at sites, mechanical tunability indicated good fidelity printability during optimized 3D printing. Moreover, act as nanotherapeutics, enhancing biological activity dressings terms reactive oxygen species scavenging, angiogenesis, anti-inflammation activity, thereby accelerating acute healing. These bioinspired hydrogels produce using biomineralization strategy an excellent platform clinical applications chronic repair.

Language: Английский

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Amorphizing Metal Selenides-Based ROS Biocatalysts at Surface Nanolayer toward Ultrafast Inflammatory Diabetic Wound Healing

ACS Nano, Journal Year: 2023, Volume and Issue: 17(3), P. 2943 - 2957

Published: Jan. 23, 2023

The microenvironments with high reactive-oxygen-species (ROS) levels, inflammatory responses, and oxidative-stress effects in diabetic ulcer wounds, leading to poor proliferation differentiation of stem cells, severely inhibit their efficient healing. Here, overcome the unbalanced multielectron reactions ROS catalysis, we develop a cobalt selenide-based biocatalyst an amorphous Ru@CoSe nanolayer for ultrafast broad-spectrum catalytic ROS-elimination. Owing enriched electrons more unoccupied orbitals Ru atoms, nanolayer-equipped displays excellent catalase-like kinetics (maximal reaction velocity, 23.05 μM s–1; turnover number, 2.00 s–1), which exceeds most currently reported metal compounds. theoretical studies show that atoms act as "regulators" tune electronic state Co sites modulate interaction oxygen intermediates, thus improving reversible redox properties active sites. Consequently, can efficiently rescue mesenchymal cells maintain angiogenic potential oxidative stress environment. In vivo experiments reveal superior ROS-elimination ability on wound. This study offers effective nanomedicine ROS-scavenging healing wounds also provides strategy design biocatalytic compounds via bringing structures.

Language: Английский

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Advances in Nanotechnology for Biofilm Inhibition

ACS Omega, Journal Year: 2023, Volume and Issue: 8(24), P. 21391 - 21409

Published: June 7, 2023

Biofilm-associated infections have emerged as a significant public health challenge due to their persistent nature and increased resistance conventional treatment methods. The indiscriminate usage of antibiotics has made us susceptible range multidrug-resistant pathogens. These pathogens show reduced susceptibility intracellular survival. However, current methods for treating biofilms, such smart materials targeted drug delivery systems, not been found effective in preventing biofilm formation. To address this challenge, nanotechnology provided innovative solutions formation by clinically relevant Recent advances nanotechnological strategies, including metallic nanoparticles, functionalized dendrimers, polymeric cyclodextrin-based delivery, solid lipid polymer conjugates, liposomes, may provide valuable technological against infectious diseases. Therefore, it is imperative conduct comprehensive review summarize the recent advancements limitations advanced nanotechnologies. present Review encompasses summary agents, mechanisms that lead formation, impact on human health. In nutshell, offers survey managing infections. A detailed presentation how these strategies improve control prevent key objective mechanisms, applications, prospects nanotechnologies better understanding

Language: Английский

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