Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137376 - 137376
Published: Feb. 1, 2025
Language: Английский
Nanomaterials, Journal Year: 2024, Volume and Issue: 14(6), P. 531 - 531
Published: March 16, 2024
Biomimetic scaffolds imitate native tissue and can take a multidimensional form. They are biocompatible influence cellular metabolism, making them attractive bioengineering platforms. The use of biomimetic adds complexity to traditional cell cultivation methods. most commonly used technique involves cultivating cells on flat surface in two-dimensional format due its simplicity. A three-dimensional (3D) provide microenvironment for surrounding cells. There two main techniques obtaining 3D structures based the presence scaffolding. Scaffold-free consist spheroid technologies. Meanwhile, scaffold contain organoids all constructs that various types scaffolds, ranging from decellularized extracellular matrix (dECM) through hydrogels one extensively studied forms potential culture up 4D bioprinted biomaterials. bioprinting is important create scaffolds. versatility this allows many different inks, mainly hydrogels, as well inorganic substances. Increasing amounts data evidence vast usage engineering personalized medicine, with area application being regeneration skin musculoskeletal systems. Recent papers also indicate increasing vivo tests products which further strengthen importance branch emphasize need extensive research safe humansbiomimetic tissues organs. In review article, we recent advancements field preceded by an overview technologies led development complex type culture.
Language: Английский
Citations
20
Bioengineering, Journal Year: 2023, Volume and Issue: 10(3), P. 287 - 287
Published: Feb. 23, 2023
The global development of technologies now enters areas related to human health, with a transition from conventional personalized medicine that is based significant extent on (bio)printing. goal this article review some the published scientific literature and highlight importance potential benefits using 3D (bio)printing techniques in contemporary also offer future perspectives research field. prepared according Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) guidelines. Web Science, PubMed, Scopus, Google Scholar, ScienceDirect databases were used search. Six authors independently performed search, study selection, data extraction. This focuses bio(printing) provides classification several categories: overcoming shortage organs transplantation, elimination problems due difference between sexes organ reducing cases rejection transplanted organs, enhancing survival patients drug development, genetic/congenital defects tissues surgery planning medical training young doctors. In particular, we each applications included along associated reports recent literature. addition, present an overview challenges need be overcome bioprinting medicine. reviewed articles lead conclusion may adopted as revolution personalized, it has huge near become gold standard healthcare world.
Language: Английский
Citations
29
Nature Reviews Bioengineering, Journal Year: 2024, Volume and Issue: 2(6), P. 486 - 500
Published: April 2, 2024
Language: Английский
Citations
14
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(15)
Published: Feb. 23, 2024
Abstract A 3D bioprinted neurovascular unit (NVU) model is developed to study glioblastoma (GBM) tumor growth in a brain‐like microenvironment. The NVU includes human primary astrocytes, pericytes and brain microvascular endothelial cells, patient‐derived cells (JHH‐520) are used for this study. Fluorescence reporters with confocal high content imaging quantitate real‐time network formation growth. Extensive validation of the NVU‐GBM immunostaining relevant cellular markers extracellular matrix components; single cell RNA sequencing (scRNAseq) establish physiologically transcriptomics changes; secretion GBM‐relevant cytokines. scRNAseq reveals changes gene expression cytokines associated wound healing/angiogenesis, including appearance an mesenchymal transition population. test 18 chemotherapeutics anti‐cancer drugs assess pharmacological relevance robustness throughput screening.
Language: Английский
Citations
13
ACS Nano, Journal Year: 2024, Volume and Issue: 18(19), P. 12168 - 12186
Published: April 30, 2024
Assessment of hypoxia, nutrients, metabolite gradients, and other hallmarks the tumor microenvironment within 3D multicellular spheroid organoid models represents a challenging analytical task. Here, we report red/near-infrared (NIR) emitting cell staining with O2-sensitive nanoparticles, which enable measurements oxygenation on conventional fluorescence microscope. Nanosensor probes, termed "MMIR" (multimodal infrared), incorporate an NIR metalloporphyrin (PtTPTBPF) deep red aza-BODIPY reference dyes biocompatible polymer shell, allowing for oxygen gradient quantification via ratio phosphorescence lifetime readouts. We optimized techniques evaluated nanosensor probe characteristics cytotoxicity. Subsequently, applied nanosensors to live based HCT116, DPSCs, SKOV3 cells, at rest, treated drugs affecting respiration. found that growth medium viscosity, size, formation method influenced oxygenation. Some spheroids produced from HCT116 dental pulp stem cells exhibited "inverted" higher core levels than periphery. This contrasted frequently encountered "normal" hypoxia toward caused by diffusion. Further microscopy analysis demonstrated metabolic stratification spheroids: thus, autofluorescence FLIM NAD(P)H indicated glycolytic localization OxPhos-active Collectively, demonstrate strong potential NIR-emitting ratiometric advanced studies targeting quantitative real-time monitoring metabolism in complex tissue models.
Language: Английский
Citations
12
Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(36)
Published: April 18, 2024
Abstract Decellularized small intestine submucosa (dSIS) is a promising biomaterial for promoting tissue regeneration. Isolated from the submucosal layer of animal jejunum, SIS rich in extracellular matrix (ECM) proteins, including collagen, laminin, and fibronectin. Following mild decellularization, dSIS becomes an acellular that supports cell adhesion, proliferation, differentiation. Conventional usually obtained by thermal crosslinking, which yields soft scaffold with low stability. To address these challenges, modified methacrylate groups photocrosslinking into stable hydrogels. However, has not been clickable handles orthogonal crosslinking. Here, development norbornene‐modified dSIS, named dSIS‐NB, via reacting amine carbic anhydride acidic aqueous reaction conditions reported. Using triethylamine (TEA) as base catalyst, high degrees NB substitution on are obtained. In addition to describing synthesis its adaptability hydrogel crosslinking cancer vascular engineering explored. Impressively, compared physically crosslinked collagen matrices, orthogonally dSIS‐NB hydrogels supported rapid dissemination cells superior vasculogenic angiogenic properties. also exploited versatile bioink 3D bioprinting.
Language: Английский
Citations
9
Engineering, Journal Year: 2024, Volume and Issue: unknown
Published: June 1, 2024
Organ damage or failure arising from injury, disease, and aging poses challenges due to the body's limited regenerative capabilities. transplantation presents issues of donor shortages immune rejection risks, necessitating innovative solutions. The 3D bioprinting organs on demand offers promise in tissue engineering medicine. In this review, we explore state-of-the-art technologies, with a focus bioink cell type selections. We follow discussions advances solid organs, such as heart, liver, kidney, pancreas, highlighting importance vascularization integration. Finally, provide insights into key future directions context clinical translation bioprinted their large-scale production.
Language: Английский
Citations
9
Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 257 - 269
Published: Sept. 4, 2024
Language: Английский
Citations
9
Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 100, P. 106033 - 106033
Published: Aug. 10, 2024
In recent years, three-dimensional (3D) spheroid technology has emerged as a crucial instrument in the realm of cancer research, offering near-physiological model for probing human cancers. By faithfully replicating organ architecture and functionality, spheroids furnish versatile platform addressing spectrum clinical biomedical inquiries, encompassing pharmacology disease pathophysiology. Their distinct advantage over conventional two-dimensional (2D) cell cultures lies their capacity to emulate 3D extracellular microenvironment attributes characteristic solid tumors, including architectural intricacies, gene expression profiles, secretion soluble mediators. Derived effectively from both normal malignant patient tissues, facilitate modeling progression, mutation dynamics, carcinogenesis pathways. Moreover, expedites drug screening processes personalized therapeutic interventions. Although challenges persist accurately recapitulating immune system within models, co-culturing with lymphocytes holds significant promise immunotherapy applications. This comprehensive examination outlines diverse methodologies establishing characterizing spheroids, highlighting extensive utilization oncology. The manuscript underscores immense paving way an uprising understanding management cancer, abundant opportunities further investigation progress treatment approaches.
Language: Английский
Citations
8
Biofabrication, Journal Year: 2024, Volume and Issue: 16(4), P. 045029 - 045029
Published: Aug. 12, 2024
Abstract Multicellular spheroids such as microtissues and organoids have demonstrated great potential for tissue engineering applications in recent years these 3D cellular units enable improved cell–cell cell–matrix interactions. Current bioprinting processes that use multicellular building blocks limited control on post printing distribution of cell or moderate throughput efficiency. In this work, we presented a laser-assisted approach able to transfer larger structures. Cartilaginous formed by human periosteum derived cells (hPDCs) were successfully bioprinted possessing high viability the capacity undergo chondrogenic differentiation printing. Smaller hPDC with diameters ranging from ∼100 150 µ m through laser-induced forward method (LIFT) however constituted challenge. For reason novel alternative was developed termed laser induced propulsion mesoscopic objects (LIPMO) whereby bioprint up 300 m. Moreover, combined process computer aided image analysis demonstrating ‘target shoot’, automated selection, multiple large single sequence. By taking advantage target shoot system, multilayered constructs containing density fabricated.
Language: Английский
Citations
8