bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 6, 2024
A
gold
nanoparticle
(AuNP)-supported
rapid,
electronic
detection
(NasRED)
platform
is
demonstrated
with
high
specificity
to
differentiate
SARS-CoV-2
from
human
coronaviruses
while
achieving
sensitivity.
Uniquely,
active
fluidic
force
exerted
on
AuNPs
in
a
microcentrifuge
tube,
through
engineered
centrifugation
and
vortex
agitation,
accelerate
signal
transduction
biochemical
protein
binding
physical
AuNP
aggregation
precipitation
finally
signals
customized,
stabilized
circuitry.
The
limits
of
are
found
<80
aM
(12
fg/mL)
∼700
(105
detect
antibodies
pooled
serum
20%
diluted
whole
blood,
<900
(45
4.1
fM
(200
nucleocapsid
proteins
saliva
nasal
fluid,
respectively.
With
small
test
volume
(6
µL),
rapid
turnaround
time
(as
fast
as
<15
min),
low
reagent
cost
(estimated
∼$2),
system
footprint,
this
novel
digital
can
be
utilized
for
accurate
diagnosis
screening
wide
range
infectious
chronic
diseases.
Chemical Science,
Journal Year:
2023,
Volume and Issue:
14(23), P. 6149 - 6206
Published: Jan. 1, 2023
The
disastrous
spread
of
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
has
induced
public
healthcare
issues
and
weakened
the
global
economy
significantly.
Although
SARS-CoV-2
infection
is
not
as
fatal
initial
outbreak,
many
infected
victims
suffer
from
long
COVID.
Therefore,
rapid
large-scale
testing
critical
in
managing
patients
alleviating
its
transmission.
Herein,
we
review
recent
advances
techniques
to
detect
SARS-CoV-2.
sensing
principles
are
detailed
together
with
their
application
domains
analytical
performances.
In
addition,
advantages
limits
each
method
discussed
analyzed.
Besides
molecular
diagnostics
antigen
antibody
tests,
also
neutralizing
antibodies
emerging
variants.
Further,
characteristics
mutational
locations
different
variants
epidemiological
features
summarized.
Finally,
challenges
possible
strategies
prospected
develop
new
assays
meet
diagnostic
needs.
Thus,
this
comprehensive
systematic
detection
technologies
may
provide
insightful
guidance
direction
for
developing
tools
diagnosis
analysis
support
effective
long-term
pandemic
management
control.
Health and Technology,
Journal Year:
2023,
Volume and Issue:
13(2), P. 273 - 284
Published: Jan. 6, 2023
Abstract
This
scoping
review
compiled
information
concerning
digital
health
technologies
(DHTs)
evolution
to
support
primary
care
(PHC)
during
COVID-19
and
lessons
for
the
future
of
PHC.
The
identified
literature
was
published
peak
years
(2019–2021),
retrieved
from
PubMed,
Scopus,
Google
Scholar,
as
well
hand
searched
on
internet.
Predefined
inclusion
criteria
were
used,
thematic
analysis
applied,
reporting
followed
PRISMA
Scoping
Reviews.
A
total
46
studies
included
in
final
synthesis
(40
articles,
one
book,
two
book
chapters,
working
paper,
technical
reports).
These
scrutinized
various
aspects
DHTs,
entailing
19
types
DHTs
with
20
areas
use
that
can
be
compressed
into
five
bigger
PHC
functions:
general
service
delivery
(teleconsultations,
e-diagnosis,
e-prescription,
etc.);
behavior
promotion
literacy
(e.g.,
combating
vaccine
hesitancy);
surveillance
functions;
vaccination
drugs;
enhancing
system
decision-making
proper
follow-up
ongoing
interventions
COVID-19.
have
potential
solve
some
problems
plagued
us
even
prior
Therefore,
this
study
uses
a
forward-looking
viewpoint
further
stimulate
evidence-based
DHT,
making
it
more
inclusive,
educative,
satisfying
people’s
needs,
both
under
normal
conditions
outbreaks.
More
research
narrowed
questions
is
needed,
particular
emphasis
quality
assurance
(standards
tools,
infrastructure,
platforms),
financial
perspectives
(payment
services
adoption
incentives).
Nature Communications,
Journal Year:
2023,
Volume and Issue:
14(1)
Published: July 10, 2023
Abstract
Real-time
surveillance
of
airborne
SARS-CoV-2
virus
is
a
technological
gap
that
has
eluded
the
scientific
community
since
beginning
COVID-19
pandemic.
Offline
air
sampling
techniques
for
detection
suffer
from
longer
turnaround
times
and
require
skilled
labor.
Here,
we
present
proof-of-concept
pathogen
Air
Quality
(pAQ)
monitor
real-time
(5
min
time
resolution)
direct
aerosols.
The
system
synergistically
integrates
high
flow
(~1000
lpm)
wet
cyclone
sampler
nanobody-based
ultrasensitive
micro-immunoelectrode
biosensor.
showed
comparable
or
better
performance
than
commercially
available
samplers.
Laboratory
experiments
demonstrate
device
sensitivity
77–83%
limit
7-35
viral
RNA
copies/m
3
air.
Our
pAQ
suited
point-of-need
variants
in
indoor
environments
can
be
adapted
multiplexed
other
respiratory
pathogens
interest.
Widespread
adoption
such
technology
could
assist
public
health
officials
with
implementing
rapid
disease
control
measures.
Science Advances,
Journal Year:
2025,
Volume and Issue:
11(2)
Published: Jan. 10, 2025
Measuring
virus
in
biofluids
is
complicated
by
confounding
biomolecules
coisolated
with
viral
nucleic
acids.
To
address
this,
we
developed
an
affinity-based
microfluidic
device
for
specific
capture
of
intact
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2).
Our
approach
used
engineered
angiotensin-converting
enzyme
to
from
plasma
and
other
complex
biofluids.
leverages
a
staggered
herringbone
pattern,
nanoparticle
surface
coating,
processing
conditions
achieve
detection
as
few
3
copies
per
milliliter.
We
further
validated
our
assay
on
103
plasma,
36
saliva,
29
stool
samples
collected
unique
patients
COVID-19,
showing
SARS-CoV-2
72%
samples.
Longitudinal
monitoring
the
revealed
device’s
capacity
ultrasensitive
active
infections
over
time.
technology
can
be
adapted
target
viruses
using
relevant
cell
entry
molecules
affinity
capture.
This
versatility
underscores
potential
widespread
application
load
disease
management.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(33), P. 11591 - 11599
Published: Aug. 10, 2022
The
COVID-19
pandemic
has
led
to
a
global
crisis
with
devastating
effects
on
public
healthcare
and
the
economy.
Sensitive
detection
of
SARS-CoV-2
is
key
diagnose
control
its
spread.
spike
(S)
protein
an
abundant
viral
transmembrane
suitable
target
for
selective
recognition
SARS-CoV-2.
Here,
we
report
that
bovine
serum
albumin
prescreening,
specific
phage
peptide
targeting
S1
was
biopanned
pIII
display
library.
identified
#2
expressing
(amino
acid
sequence:
NFWISPKLAFAL)
shows
high
affinity
dissociation
constant
3.45
±
0.58
nM.
Furthermore,
good
specificity
binding
site
at
N-terminal
domain
subunit
through
hydrogen
bond
network
hydrophobic
interaction,
supported
by
molecular
docking.
Then,
sandwiched
phage-based
enzyme-linked
chemiluminescence
immunoassay
(ELCLIA)
established
using
as
bifunctional
probe
capable
antigen
signal
amplification.
After
optimizing
conditions,
proposed
ELCLIA
exhibited
sensitivity,
low
78
pg/mL
could
be
detected.
This
method
can
applied
detect
60
transducing
units
(TU)/mL
pseudovirus
in
50%
saliva.
Therefore,
peptides
have
prospects
powerful
biological
probes
biomedical
applications.
Biosensors,
Journal Year:
2022,
Volume and Issue:
12(9), P. 762 - 762
Published: Sept. 16, 2022
Nanomechanical
sensors
have
gained
significant
attention
as
powerful
tools
for
detecting,
distinguishing,
and
identifying
target
analytes,
especially
odors
that
are
composed
of
a
complex
mixture
gaseous
molecules.
their
arrays
promising
platform
artificial
olfaction
in
combination
with
data
processing
technologies,
including
machine
learning
techniques.
This
paper
reviews
the
background
nanomechanical
sensors,
conventional
cantilever-type
sensors.
Then,
we
focus
on
one
optimized
structures
static
mode
operation,
Membrane-type
Surface
stress
Sensor
(MSS),
discuss
recent
advances
MSS
applications
towards
olfaction.
