Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 419, P. 136423 - 136423
Published: Aug. 6, 2024
Language: Английский
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 173, P. 117629 - 117629
Published: March 5, 2024
Language: Английский
Citations
20
Chemical Science, Journal Year: 2023, Volume and Issue: 14(23), P. 6149 - 6206
Published: Jan. 1, 2023
The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal initial outbreak, many infected victims suffer from long COVID. Therefore, rapid large-scale testing critical in managing patients alleviating its transmission. Herein, we review recent advances techniques to detect SARS-CoV-2. sensing principles are detailed together with their application domains analytical performances. In addition, advantages limits each method discussed analyzed. Besides molecular diagnostics antigen antibody tests, also neutralizing antibodies emerging variants. Further, characteristics mutational locations different variants epidemiological features summarized. Finally, challenges possible strategies prospected develop new assays meet diagnostic needs. Thus, this comprehensive systematic detection technologies may provide insightful guidance direction for developing tools diagnosis analysis support effective long-term pandemic management control.
Language: Английский
Citations
38
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(33), P. 12532 - 12540
Published: Aug. 8, 2023
Rapid and sensitive antigen detection using a lateral flow immunoassay (LFIA) is crucial for diagnosing infectious diseases due to its simplicity, speed, user-friendly features. However, it remains critical issue explore specific biorecognition elements powerful signal amplification. In this study, taking SARS-CoV-2 as proof of concept, peptide, WFLNDSELIML, binding the spike (S) was identified by nonamplified biopanning method, which exhibited high affinity target, with dissociation constant 9.29 ± 1.55 nM. Molecular docking analysis reveals that peptide binds N-terminal domain S antigen. Then, capture probe angiotensin-converting enzyme 2 probe, peptide-based (pLFIA) without any antibody developed, polydopamine nanosphere (PDA)@MnO2 nanocomposite excellent oxidase-like activity used colorimetric label, exhibiting dual-mode remarkable amplification natural melanin on-demand nanozyme catalytic enhancement. The PDA@MnO2-based pLFIA capable detecting limit 8.01 pg/mL, 18.7 times lower than conventional tagged gold nanoparticles. Additionally, as-proposed can detect up 150 transduction units/mL pseudoviruses spiked in saliva samples. Given outstanding analytical performance, proposed may offer reliable option rapid diagnosis SARS-CoV-2.
Language: Английский
Citations
33
Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1), P. 36 - 45
Published: Jan. 17, 2024
As the most complex post-translational modification, glycosylation involves covalent linking of glycan(s) to a biomolecule. The proteins, peptides, lipids, and nucleic acids results in formation diverse glycoconjugates, like glycoproteins, peptidoglycans, glycopeptides, glycolipids, lipopolysaccharides, glycoRNAs, glycoconjugates are also ubiquitous on surface extracellular vesicles (EVs), cells, bacteria, viruses. conjugated glycans integral cell recognition (e.g., adhesion homing), trafficking, immune recognition, hormone-receptor binding, protein folding stabilization, many other biological processes. Currently, glycan-conjugated objects have found widespread applications disease diagnosis exosomes, circulating tumor cells) therapy therapeutic monoclonal antibodies). Over past decades, variety methods been established for quantitative assay objects, light their significant diagnostic values, using antibodies, aptamers, or affinity peptides as ligands. Relative methods, aptamer-based electrochemical biosensors (i.e., aptasensors) attracted much attention recent years detection targets, by virtue intrinsic advantages such low cost, good portability, simple instrument, superior stability anti-interference capability, high sensitivity selectivity. This review summarizes biological, diagnostic, significance then presents progress aptasensing including lipopolysaccharides (LPSs), cells (CTCs), pathogenic microbes bacteria viruses). Moreover, challenges future prospects discussed.
Language: Английский
Citations
9
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(24), P. 10064 - 10073
Published: June 6, 2024
The global spread of monkeypox has become a worldwide public healthcare issue. Therefore, there is an urgent need for accurate and sensitive detection methods to effectively control its spreading. Herein, we screened by phage display two peptides M4 (sequence: DPCGERICSIAL) M6 SCSSFLCSLKVG) with good affinity specificity virus (MPXV) B21R protein. To simulate the state peptide in avoid spatial obstacles peptide, GGGSK was added at C terminus named as M4a. Molecular docking shows that M4a are bound different epitopes hydrogen bonds salt-bridge interactions, respectively. Then, selected capture probe, carbonized polymer dots (CPDs) fluorescent colorimetric double-signal peptide/antigen/signal peptide-displayed sandwich ELISA triggered horseradish peroxidase (HRP) through simple internal filtration effect (IFE) constructed. HRP catalyzes H2O2 oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) generate blue oxidized TMB, which can further quench fluorescence CPDs IFE, enabling detect MPXV modes. proposed immunoassay platform sensitivity reliability detection. limit modes 27.8 9.14 pg/mL B21R, Thus, established double-peptide sandwich-based dual-signal provides guidance development reliable antigen capable mutual confirmation, also great potential exploring various analytical strategies other respiratory surveillance.
Language: Английский
Citations
9
Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 222, P. 114909 - 114909
Published: Nov. 15, 2022
Language: Английский
Citations
31
Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 387, P. 133746 - 133746
Published: March 29, 2023
Language: Английский
Citations
22
Analytical and Bioanalytical Chemistry, Journal Year: 2023, Volume and Issue: 415(18), P. 3927 - 3944
Published: March 3, 2023
Language: Английский
Citations
21
Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1264, P. 341300 - 341300
Published: April 29, 2023
Language: Английский
Citations
16
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(30), P. 11359 - 11364
Published: July 19, 2023
Signal amplification techniques are highly desirable for the analysis of low-level targets that closely related with diseases and monitoring important biological processes. However, it is still challenging to achieve this goal in a facile economical way. Herein, we developed novel dual signal strategy by combining urease catalysis release Ag+ from silver nanoparticles (AgNPs). This was used quantifying DNA sequence (HIV-1) human immunodeficiency virus (HIV). target HIV-1 hybridizes capture probe on magnetic beads reporter AgNPs, forming sandwich complex. The captured AgNPs then transformed into numerous ions inactivate ureases. Without catalytic production ammonia urea, substrate solution shows low pH 5.8 will increase otherwise. change monitored indicator (phenol red), which allows colorimetric detection. proposed approach sensitive, easy use, economic, universal, exhibiting detection limit 9.7 fM (i.e., 1.94 attomoles) dynamic linear range 4 orders
Language: Английский
Citations
16