Top-Down Computational Design of Molecule Recognition Peptides (MRPs) for Enzyme-Peptide Self-Assembly and Chemiluminescent Biosensing DOI
Lihong Yu,

Chenglin Yang,

Shuting Cheng

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 20, 2024

The recognition of small molecules plays a crucial role in disease diagnosis, environmental assessment, and food safety. Currently, their elements predominantly rely on antibodies aptamers while suffering from limitation the complex screening process due to low immunogenicity molecules. Herein, we present top-down computational design strategy for molecule peptides (MRPs) enzyme-peptide self-assembly chemiluminescence biosensing. Taking ochratoxin A (OTA) as an illustrative example, human serum albumin (HSA) was selected parental protein its high affinity OTA binding. Through iterative simulations involving binding domain HSA-OTA complex, our identified specific 15-mer MRP (RLKCASLKFGERAFK), which possesses excellent (38.02 ± 1.24 nM) against OTA. Molecular dynamics revealed that unfolds into flexible short chain with OTA, but exhibits weak or no five structurally similar mycotoxins. Furthermore, developed novel approach mediated by calcium(II) obtain nanoflowers, integrates both element (MRP) signal translator (enzyme) assembled nanoflowers allow MRPs be directly utilized tracer biosensing without labeling secondary antibodies. This computational-to-application offers new route small-molecule recognition.

Language: Английский

New advances in the diagnosis and treatment of autism spectrum disorders DOI Creative Commons
Lei Qin, Haijiao Wang,

Wenjing Ning

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: June 10, 2024

Language: Английский

Citations

14
Cortisol: Biosensing and detection strategies DOI

Sesuraj Balasamy,

Raji Atchudan, Sandeep Arya

et al.

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 562, P. 119888 - 119888

Published: July 24, 2024

Language: Английский

Citations

10
Molecular modulation of aggregation-induced luminescence for improving response sensing of DNA hydrogels DOI

Xuebing Bai,

Chenxi Wang, Lei Huang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: 276, P. 117254 - 117254

Published: Feb. 12, 2025

Language: Английский

Citations

1
Emerging strategies in fluorescent aptasensor toward food hazard aflatoxins detection DOI

Mengke Zhang,

Xiaodong Guo

Trends in Food Science & Technology, Journal Year: 2022, Volume and Issue: 129, P. 621 - 633

Published: Nov. 1, 2022

Language: Английский

Citations

32
Controllable‐Swelling Microneedle–Assisted Ultrasensitive Paper Sensing Platforms for Personal Health Monitoring DOI

Yi‐Chia Hsieh,

Chih‐Yu Lin, Hsin‐Yao Lin

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(24)

Published: April 11, 2023

Microneedle (MN) patches, which allow the extraction of skin interstitial fluid (ISF) without a pain sensation, are powerful tools for minimally invasive biofluid sampling. Herein, an MN-assisted paper-based sensing platform that enables rapid and painless analysis with ultrasensitive molecular recognition capacity is developed. First, controllable-swelling MN patch constructed through engineering poly(ethylene glycol) diacrylate/methacrylated hyaluronic acid hydrogel; it combines rapid, sufficient ISF excellent structural integrity. Notably, analyte molecules in needles can be recovered into moist cellulose paper spontaneous diffusion. More importantly, functionalized enzymatic colorimetric reagents or plasmonic array, enabling desired detection capacity-for example, use surface-enhanced Raman spectroscopy sensors leads to label-free, trace (sub-ppb level) diverse set (cefazolin, nicotine, paraquat, methylene blue). Finally, nicotine selected as model drug evaluate monitoring three human volunteers. The changes levels tracked, varying significantly response metabolism different This as-designed system should open up new opportunities precision medicine, especially personal healthcare monitoring.

Language: Английский

Citations

22
Integrated lighting ergonomics: A review on the association between non-visual effects of light and ergonomics in the enclosed cabins DOI
Zhiming Gou,

Bingchen Gou,

Wenyi Liao

et al.

Building and Environment, Journal Year: 2023, Volume and Issue: 243, P. 110616 - 110616

Published: July 13, 2023

Language: Английский

Citations

19
Advanced biosensors for mycotoxin detection incorporating miniaturized meters DOI

Mengke Zhang,

Xiaodong Guo, Jiaqi Wang

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 224, P. 115077 - 115077

Published: Jan. 11, 2023

Language: Английский

Citations

16
Progress in salivary glands: Endocrine glands with immune functions DOI Creative Commons

Yu Feng Shang,

Yi Shen,

Meng Chen Zhang

et al.

Frontiers in Endocrinology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 3, 2023

The production and secretion of saliva is an essential function the salivary glands. Saliva a complicated liquid with different functions, including moistening, digestion, mineralization, lubrication, mucosal protection. This review focuses on mechanism neural regulation secretion, secreted in response to various stimuli, odor, taste, vision, mastication. chemical physical properties change dynamically during physiological pathophysiological processes. Moreover, central nervous system modulates via neurotransmitters neuroreceptors. Smell, taste have been investigated for connection between salivation brain function. immune endocrine functions glands explored recently. Salivary play role innate adaptive immunity Various cells such as B cells, T macrophages, dendritic well immunoglobins like IgA IgG found Evidence supports synthesis corticosterone, testosterone, melatonin contains many potential biomarkers derived from epithelial gingival crevicular fluid, serum. High level matrix metalloproteinases cytokines are markers oral carcinoma, infectious disease cavity, systemic disease. Further research required monitor predict health clinical practice precision medicine.

Language: Английский

Citations

15
Is burning mouth syndrome associated with stress? A meta‐analysis DOI Creative Commons
André Luís Porporatti, Ângela Graciela Deliga Schröder,

Ashley Lebel

et al.

Journal of Oral Rehabilitation, Journal Year: 2023, Volume and Issue: 50(11), P. 1279 - 1315

Published: June 19, 2023

Some studies have shown burning mouth syndrome (BMS) as comorbid psychosocial and psychiatric disorders, well, pointed at stress a major risk factor.The aim of this meta-analysis was to answer the following question: 'Is there an association between BMS stress, compared healthy controls?'Two reviewers searched for effect in published on five main databases three from grey literature. Various questionnaires biomarkers were analysed. Of 2489 selected articles, 30 met inclusion criteria. Studies englobed questionnaires, such Perceived Stress Questionnaire, Lipp Symptoms Inventory, Holmes-Rahe scale, Depression, Anxiety, Scale (DASS-21), Recent Experience Test; various biomarkers, cortisol, opiorphin, IgA, α-amylase interleukins.In all with significantly increased group vs. control. Patients presented 25.73% higher cortisol levels, 28.17% IgA levels 40.62% than controls. Meta-analysis found that subjects 3.01 nmoL/L [0.53; 5.50] 84.35 kU/L [15.00; 153.71] 29.25 mg/mL [9.86; 48.64] 258.59 pg/mL [59.24; 457.94] IL-8 No differences opiorphin concentration ng/mL [-0.96; 2.53]. For interleukins, no founded IL-1 β, IL-2, IL-4, IL-6, IL-8, IL-10 TNF-α.Based available evidence, suggests more factors questionnaire-based studies, α-amylase,

Language: Английский

Citations

13
Integrating Assessment of Circadian Rhythmicity to Improve Treatment Outcomes for Circadian Rhythm Sleep-Wake Disorders: Updates on New Treatments DOI Creative Commons

Gabrielle F. Gloston,

Kristina M. Ward,

G. Carolina Rodriguez-Torres

et al.

Current Sleep Medicine Reports, Journal Year: 2025, Volume and Issue: 11(1)

Published: Feb. 17, 2025

Abstract Purpose of Review Despite advancements in basic circadian research, development new diagnostic and treatment strategies for rhythm sleep-wake disorders (CRSWDs) has been slow. Here, we review the most recent innovations human assessment emerging therapies CRSWDs. Recent Findings Researchers have improved existing methods to overcome logistical barriers developed novel methods. New treatments CRSWDs involve pharmacological behavioral that modulate phase, amplitude, and/or robustness central clock. Summary Commercialization these tools will require overcoming barriers, such as additional testing confirm underlying pathology mechanism action potential treatments. Clinicians scientists are also called survey adjacent fields adopt may offer clarity Lastly, must continue advocate medical insurance coverage current future technologies improve patient care.

Language: Английский

Citations

0