LL-37_Renalexin hybrid peptide exhibits antimicrobial activity at lower MICs than its counterpart single peptides DOI Creative Commons
Julius Kwesi Narh, Néstor Casillas-Vega, Xristo Zárate

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Jan. 13, 2024

Abstract An alarming global public health and economic peril has been the emergence of antibiotic resistance resulting from clinically relevant bacteria pathogens, including Enterococcus faecium , Staphylococcus aureus Klebsiella pneumonia Acinetobacter baumannii Pseudomonas aeruginosa Enterobacter species constantly exhibiting intrinsic extrinsic mechanisms against last-resort antibiotics like gentamycin, ciprofloxacin, tetracycline, colistin, standard ampicillin prescription in clinical practices. The discovery applications antimicrobial peptides (AMPs) with antibacterial properties have considered proven as alternative agents to antibiotics. In this study, we designed, produced, purified a recombinant novel multifunctional hybrid peptide LL-37_Renalexin for first time via application newly designed flexible GS linker coupled use our previously characterized small metal-binding proteins SmbP CusF3H+ carrier that allow an enhanced bacterial expression, using BL21(DE3) SHuffle T7(DE3) Escherichia coli strains, purification immobilized metal affinity chromatography. tag-free exhibited above 85% reduction colony-forming units broad-spectrum effects Methicillin-resistant (MRSA), pneumoniae isolates at lower minimum inhibition concentration level (10–33 μM) compared its counterpart single-AMPs LL-37 Renalexin (50–100 μM). Key points • linker. was expressed CusF3H+. shows potency isolates.

Language: Английский

Carbon Dots as Advanced Drug-Delivery Nanoplatforms for Antiinflammatory, Antibacterial, and Anticancer Applications: A Review DOI

Jin Qi,

Ran Zhang, Xiaoming Liu

et al.

ACS Applied Nano Materials, Journal Year: 2023, Volume and Issue: 6(11), P. 9071 - 9084

Published: May 19, 2023

Carbon dots (CDs), as rapidly developing nanomaterials, give new prospects for the treatment of various diseases. Their great biocompatibility and wide specific surface area make CDs excellent drug-delivery vehicles because they improve cellular uptake absorption allow medications to bind more easily. offer enormous potential delivery systems antiinflammatory medications. The activities drugs delivered by are higher than those free drugs. They also have controlled release, thus enhancing cumulative effect at inflammation site improving pharmacokinetics. Compared with small-molecule antibacterial medicines, CD-based efficacies, better targeting, increased internalization efficiency, decreased systemic side effects. benefits using anticancer drug carriers include high loading, lower dosage, regulated synergistic photothermal therapy, which offers a novel approach diagnosis cancer in clinical settings. However, it is important consider biosafety, toxicity, impact on immune system an organism before them therapy. This review discusses use antiinflammatory, antibacterial, summarizes current advantages challenges delivery.

Language: Английский

Citations

27
Sustainable utilization of proteins from fish processing by-products: Extraction, biological activities and applications DOI

Ziyi Yuan,

Xingqian Ye,

Zhiqiang Hou

et al.

Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 143, P. 104276 - 104276

Published: Nov. 24, 2023

Language: Английский

Citations

26
Charged Fibrous Dressing to Promote Diabetic Chronic Wound Healing DOI
Xiaomeng Yang, Wěi Li,

Youmei Liu

et al.

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 13(2)

Published: Oct. 13, 2023

Diabetic chronic wounds cause a significant amount of pain to patients because their low cure rates and high recurrence rates. Traditional approaches treating diabetic often involve the delivery drugs or cytokines that regulate microenvironment eliminate bacterial infection in wound area, but they are passive controlling cell behaviors may lead drug resistance. Emerging drug-free treatments important for convenient, effective, safe treatment strategies. However, current cannot fully promote tissue regeneration prevent infections. Here, efficacy negatively charged fiber dressing promoting healing is investigated. The can generate reactive oxygen species inhibit reproduction with assistance ultrasound during inflammatory phase. Furthermore, provides an electrostatic field regulates cellular behavior proliferative phases. In particular, fibroblast migration induce macrophage polarization neovascularization without any additional drugs. It demonstrated this strategy enables mouse model, achieving effective closure over 12-day cycle providing therapeutic care.

Language: Английский

Citations

22
Antimicrobial peptides as antimicrobials for wound care management: A comprehensive review DOI
Kunal Agam Kanaujia, Nidhi Mishra,

P.S. Rajinikanth

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 95, P. 105570 - 105570

Published: March 12, 2024

Language: Английский

Citations

10
Directed osteogenic differentiation of human bone marrow mesenchymal stem cells via sustained release of BMP4 from PBVHx-based nanoparticles DOI
Xiaoyun Huang,

Xiaoxiang Zhou,

Hui Yang

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 265, P. 130649 - 130649

Published: March 5, 2024

Language: Английский

Citations

9
A Bodipy‐Based Aggregation‐Induced Emission Nanoagent for Sonodynamic Antibacterial Studies DOI
Yanchao Xu, Dongsheng Tang, Lei‐Jiao Li

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(33)

Published: March 22, 2024

Abstract Sonodynamic therapy (SDT) has demonstrated significant potential in addressing multidrug‐resistant bacterial infections due to its noninvasive nature and independence from antibiotics light sources. However, the majority of current sonosensitizers exhibit aggregation‐caused quenching (ACQ), leading low reactive oxygen species (ROS) generation efficiency. Herein, a novel nonplanar structured sonodynamic (4,4‐difluoro‐4‐boron‐3a,4‐diaza‐indandiene) BODIPY derivative, BODN, with aggregation‐induced emission (AIE) properties, rapid substantial ROS by ultrasound is introduced. The BODN co‐assembled commercial amphiphilic polymer, mDSPE‐PEG 2000 , forming nanoparticles (BODN‐NP). Under conditions, BODN‐NP demonstrates potent antimicrobial activity improves inflammatory response promote healing wounds infected both Staphylococcus aureus methicillin‐resistant . This work expands use AIE materials provides new perspectives for innovative solutions against infections.

Language: Английский

Citations

9
Antimicrobial Peptides, Their Production, and Potential in the Fight Against Antibiotic-Resistant Pathogens DOI Creative Commons
Margarita Saubenova, Alexander Rapoport, Zhanerke Yermekbay

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(1), P. 36 - 36

Published: Jan. 17, 2025

The article reviews the literature on antimicrobial peptides (AMPs) that exhibit unique mechanisms, such as broad-spectrum activity, low development of resistance, and ability to modulate immune response host organism. Information is provided significant potential AMPs in fight against pathogens threatening human health food safety. Enrichment diet with biologically active obtained using proteolytic activity lactic acid bacteria (LAB) proposed a simple, accessible, viable alternative antibiotics does not have harmful side effect. review briefly covers methods for obtaining features LAB system responsible producing bioactive environment. It has been shown various strains makes it possible produce high-quality whey-based beverages different directions antagonistic opportunistic helps optimize gastrointestinal microbiota. assumed drinks can reduce dose antimicrobials combined therapy infectious diseases be preventive measure contagion spread resistance.

Language: Английский

Citations

1
Carbon Cycle of Polyhydroxyalkanoates (CCP): Biosynthesis and Biodegradation DOI
Siqin Zhang, Huishu Yuan, Xue Ma

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 120904 - 120904

Published: Jan. 1, 2025

Language: Английский

Citations

1
Polyhydroxyalkanoates: the natural biopolyester for future medical innovations DOI
Zi-Wei Ren, Zeyu Wang,

Yanwen Ding

et al.

Biomaterials Science, Journal Year: 2023, Volume and Issue: 11(18), P. 6013 - 6034

Published: Jan. 1, 2023

Polyhydroxyalkanoates (PHAs), a family of natural microbial biopolyesters via with excellent biodegradability and biosafety, can be produced optimally synthetic biology designed to various medical devices for applications.

Language: Английский

Citations

16
Diversity of Antimicrobial Peptides in Silkworm DOI Creative Commons
Pooja Makwana, Kamidi Rahul, Katsuhiko Ito

et al.

Life, Journal Year: 2023, Volume and Issue: 13(5), P. 1161 - 1161

Published: May 11, 2023

Antimicrobial resistance is a phenomenon that the present-day world witnessing poses serious threat to global health. The decline in development of novel therapeutics over last couple decades has exacerbated situation further. In this scenario, pursuit new alternative commonly used antibiotics gained predominance amongst researchers across world. peptides (AMPs) from natural sources have drawn significant interest recent years as promising pharmacological substitutes conventional antibiotics. most notable advantage AMPs microorganisms cannot develop them. Insects represent one potential AMPs, which are synthesized part an innate immune defence against invading pathogens. different insects been extensively studied, and silkworm Diverse classes (including attacins, cecropins, defensins, enbocins, gloverins, lebocins moricins) were identified exhibit antimicrobial property bacteria, fungi viruses, indicating their therapeutic benefits. This review briefs about responses pathogens, isolation silkworms, reported silkworms activity various microorganisms.

Language: Английский

Citations

13