The Complex Link and Disease Between the Gut Microbiome and the Immune System in Infants DOI Creative Commons
Huan Zhang, Zhilin Zhang, Yiqun Liao

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: June 15, 2022

The human gut microbiome is important for health. development of stable microbial communities in the gastrointestinal tract closely related to early growth and host immunity. After birth a baby, immune cells mature parallel adapt complex environment. linked system influences each other. This interaction associated with various diseases infants young children, such as asthma, food allergies, necrotizing colitis, obesity, inflammatory bowel disease. Thus, composition infant can predict risk disease progression. At same time, be regulated many ways used prevent treat by modulating microbiome. most impacts on microbiota are maternal, including delivery feeding. differences reflect maternal microbiota, which turn reflects given population, clinically significant.

Language: Английский

Probiotics Function in Preventing Atopic Dermatitis in Children DOI Open Access
Caterina Anania, Giulia Brindisi,

Ivana Martinelli

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(10), P. 5409 - 5409

Published: May 12, 2022

Atopic dermatitis (AD) is a chronic inflammatory skin disorder characterized by relapsing eczematous injuries and severe pruritus. In the last few years, AD prevalence has been increasing, reaching 20% in children 10% adults high-income countries. Recently, potential role of probiotics prevention generated considerable interest. As many clinical studies show, gut microbiota able to modulate systemic immune responses influencing development sensitization allergy. Probiotics are used increasingly against AD. However, molecular mechanisms underlying mediated anti-allergic effect remain unclear there controversy about their efficacy. this narrative review, we examine actual evidence on probiotic supplementation for pediatric population, discussing also biological action regard.

Language: Английский

Citations

37
Recent progress on health effects and biosynthesis of two key sialylated human milk oligosaccharides, 3′-sialyllactose and 6′-sialyllactose DOI
Yingying Zhu, Jiameng Zhang, Wenli Zhang

et al.

Biotechnology Advances, Journal Year: 2022, Volume and Issue: 62, P. 108058 - 108058

Published: Nov. 11, 2022

Language: Английский

Citations

37
Pathway Optimization and Uridine 5′-Triphosphate Regeneration for Enhancing Lacto-N-Tetraose Biosynthesis in Engineered Escherichia coli DOI
Zeyu Li, Yingying Zhu, Pan Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(25), P. 7727 - 7735

Published: June 20, 2022

Recently, human milk oligosaccharides (HMOs) have attracted increasing attention and display great commercial importance, especially for the infant formula industry. Lacto-N-tetraose (LNT) is an important neutral HMO commercially added in a core structure synthesizing complex HMOs. Previously, novel LNT-generating β-1,3-galactosyltransferase from Pseudogulbenkiania ferrooxidans was identified used construction of LNT-producing engineered Escherichia coli. In this work, LNT biosynthesis further enhanced by pathway optimization uridine 5'-triphosphate (UTP) regeneration. The main strategies included genomic integration UDP-glucose 4-epimerase-encoding gene, fine-tuning pathway-related genes, blocking competitive pathways related to UDP-galactose, overexpression UTP supply genes. maximal titer reached 6.16 57.5 g/L shake-flask fed-batch fermentation, respectively.

Language: Английский

Citations

31
Effects of Human Milk Oligosaccharides in Infant Health Based on Gut Microbiota Alteration DOI
Miaomiao Hu, Mengli Li, Chenchen Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(2), P. 994 - 1001

Published: Jan. 5, 2023

The primary active components of breast milk are human oligosaccharides (HMOs). HMOs provide many benefits to infants, including regulating their metabolism, immune system, and brain development. Recent studies have emphasized that act as prebiotics by the metabolism intestinal microorganisms produce short-chain fatty acids, which crucial for infant In addition, with different structural characteristics can form microbial compositions. HMOs-induced predominant microbes, Bifidobacterium infantis, B. bifidum, breve, longum, metabolites demonstrated pertinent health-promoting properties. Meanwhile, could also directly reduce occurrence diseases through effects preventing pathogen infection. this review, we address probable function inside HMOs-gut microbiota-infant network, describing physiological functions implications diet on network.

Language: Английский

Citations

17
Efficient Biosynthesis of Difucosyllactose via De Novo GDP-l-Fucose Pathway in Metabolically Engineered Escherichia coli DOI

Shanquan Liang,

Zi He,

Dan Liu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(8), P. 4367 - 4375

Published: Feb. 20, 2024

Difucosyllactose (DFL) is an important component of human milk oligosaccharides (HMOs) and has significant benefits for the growth development infants. So far, a few microbial cell factories have been constructed production DFL, which still problems low high cost. Herein, high-level de novo pathway DFL-producing strain was by multistep optimization strategies in Escherichia coli BL21star(DE3). We first efficiently synthesized intermediate 2′-fucosyllactose (2′-FL) E. BL21star(DE3) advisable stepwise strategy. The truncated α-1,3/4-fucosyltransferase (Hp3/4FT) then introduced into engineered to achieve biosynthesis DFL. ATP-dependent protease (Lon) GDP-mannose hydrolase (NudK) were deleted, mannose-6-phosphate isomerase (ManA) overexpressed improve GDP-l-fucose accumulation. regulator RcsA fine-tune expression level genes, thereby increasing synthesis final produced 6.19 g/L DFL shake flask 33.45 5 L fermenter, highest reported titers so far. This study provides more economical, sustainable, effective strategy produce fucosylated (HMOs).

Language: Английский

Citations

8
Metabolic Engineering of Escherichia coli for High-Titer Biosynthesis of 3′-Sialyllactose DOI
Chenchen Li, Mengli Li, Miaomiao Hu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(10), P. 5379 - 5390

Published: Feb. 29, 2024

3′-Sialyllactose (3′-SL) is among the foremost and simplest sialylated breast milk oligosaccharides. In this study, an engineered Escherichia coli for high-titer 3′-SL biosynthesis was developed by introducing a multilevel metabolic engineering strategy, including (1) introduction of precursor CMP-Neu5Ac synthesis pathway high-performance α2,3-sialyltransferase (α2,3-SiaT) genes into strain BZ to achieve de novo 3′-SL; (2) optimizing expression glmS-glmM-glmU involved in UDP-GlcNAc pathways, constructing glutamine cycle system, balancing pools; (3) analysis critical intermediates inactivation competitive redirect carbon flux biosynthesis; (4) enhanced catalytic performance rate-limiting enzyme α2,3-SiaT RBS screening, protein tag cloning. The final BZAPKA14 yielded 9.04 g/L shake flask. 3 L bioreactor, fed-batch fermentation generated 44.2 3′-SL, with overall yield lactose conversion 0.53 g/(L h) 0.55 mol 3′-SL/mol, respectively.

Language: Английский

Citations

8
Gut microbiota: A key role for human milk oligosaccharides in regulating host health early in life DOI

Qingxue Chen,

Ma Xinming,

Zhengtao Guo

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2024, Volume and Issue: 23(5)

Published: Aug. 21, 2024

Human milk oligosaccharides (HMOs) are an evolutionarily significant advantage bestowed by mothers for facilitating the development of infant's gut microbiota. They can avoid absorption in stomach and small intestine, reaching colon successfully, where they engage close interactions with microbes. This process also enables HMOs to exert additional prebiotic effects, including regulating mucus layer, promoting physical growth brain development, as well preventing mitigating conditions such NEC, allergies, diarrhea. Here, we comprehensively review primary ways which microbiota, Bifidobacteria other genera, utilize HMOs, classify them into five central pathways. Furthermore, emphasize metabolic benefits bacteria consuming particularly recently identified intrinsic link between conversion tryptophan indole its derivatives. We examine extensive probiotic roles their recent research advancements, specifically concentrating on unsummarized role interaction microbiota becomes crucial. Additionally, delve principal tools used functional mining new HMOs. In conclusion, our study presents a thorough analysis mechanism emphasizing cooperative utilization provides overview subsequent effects this interaction. insights will inform mechanisms function.

Language: Английский

Citations

7
An In Vitro Colonic Fermentation Study of the Effects of Human Milk Oligosaccharides on Gut Microbiota and Short-Chain Fatty Acid Production in Infants Aged 0–6 Months DOI Creative Commons
Menglu Li, Han Lu, Yuling Xue

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(6), P. 921 - 921

Published: March 18, 2024

The impact of five human milk oligosaccharides (HMOs)—2′-fucosyllactose (2FL), 3′-sialyllactose (3SL), 6′-sialyllactose (6SL), lacto-N-tetraose (LNT), and lacto-N-neotetraose (LNnT)—on the gut microbiota short-chain fatty acid (SCFA) metabolites in infants aged 0–6 months was assessed through vitro fermentation. Analyses influence different HMOs on composition distribution infant SCFA levels were conducted using 16S rRNA sequencing, quantitative real-time PCR (qPCR), gas chromatography (GC), respectively. findings indicated crucial role initial shaping fermentation outcomes. Fermentation maintained dominant genera species intestine but influenced their abundance distribution. Most 10 Bifidobacteria strains effectively utilized or degradation products, particularly demonstrating proficiency utilizing 2FL sialylated compared to non-fucosylated neutral HMOs. Moreover, our study B. infantis-dominant breve-dominant as inocula revealed varying acetic produced by upon HMO degradation. Specifically, strain yielded notably higher than (p = 0.000), with minimal propionic butyric production observed at fermentation’s conclusion. These suggest potential utilization developing microbiota-targeted foods for infants.

Language: Английский

Citations

6
Strategies for Enhancing Microbial Production of 2′-Fucosyllactose, the Most Abundant Human Milk Oligosaccharide DOI
Yuanlin Liu, Yingying Zhu, Hao Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2022, Volume and Issue: 70(37), P. 11481 - 11499

Published: Sept. 12, 2022

Human milk oligosaccharides (HMOs), a group of structurally diverse unconjugated glycans in breast milk, act as important prebiotics and have plenty unique health effects for growing infants. 2′-Fucosyllactose (2′-FL) is the most abundant HMO, accounting approximately 30%, among 200 identified HMOs with different structures. 2′-FL can be enzymatically produced by α1,2-fucosyltransferase, using GDP-l-fucose donor lactose acceptor. Metabolic engineering strategies been widely used enhancement supply microbial production high productivity. enhanced two main pathways, including de novo salvage pathways. 2′-FL-producing α1,2-fucosyltransferases from various microorganisms. pathways synthesis basically constructed enhancing introducing α1,2-fucosyltransferase. Various attempted to enhance production, such acceptor enhancement, improvement functional expression In this review, current progress bacterial described detail, metabolic are comprehensively reviewed, future research focuses biotechnological suggested.

Language: Английский

Citations

28
Efficient Production of a Functional Human Milk Oligosaccharide 3′-Sialyllactose in Genetically Engineered Escherichia coli DOI
Jiameng Zhang, Yingying Zhu, Wenli Zhang

et al.

ACS Synthetic Biology, Journal Year: 2022, Volume and Issue: 11(8), P. 2837 - 2845

Published: July 8, 2022

3′-Sialyllactose (3′-SL) is one of the most important and simplest sialylated human milk oligosaccharides. In this study, a plasmid-based pathway optimization along with chromosomal integration strategies was applied for 3′-SL production. Specifically, precursor CMP-Neu5Ac synthesis genes α2,3-sialyltransferase-encoding gene were introduced into Escherichia coli BL21(DE3)ΔlacZ to realize synthesis. Genes nanA nanK involved in Neu5Ac catabolism further deleted reduce metabolic flux competitive pathway. Several α2,3-sialyltransferases from different species selected evaluate sialylation effect. The pools balanced improved by optimizing key enzyme expression UDP-GlcNAc Finally, an additional α2,3-sialyltransferase cassette integrated chromosome maximize synthesis, 4.5 g/L extracellular produced at shake-flask level. concentration raised 23.1 5 L bioreactor fermentation, which represents highest value ever reported.

Language: Английский

Citations

26