Chemical Engineering Journal,
Journal Year:
2022,
Volume and Issue:
454, P. 140066 - 140066
Published: Oct. 29, 2022
•
The
3D
interior
organo-hotspots
was
demonstrated
via
electrochemical
deposition
onto
Au
nanodimple
platforms
with
viral
lysates.
proposed
platform
exhibited
the
label-free
SERS
detection
of
SARS-CoV-2
traced
real
clinical
samples
in
a
sensitive,
quantitative,
and
reliable
manner.
In
recent
decades,
biomedical
sensors
based
on
surface-enhanced
Raman
spectroscopy
(SERS),
which
reveals
unique
spectral
features
corresponding
to
individual
molecular
vibrational
states,
have
attracted
intensive
attention.
However,
lack
system
for
precisely
guiding
biomolecules
active
hotspot
regions
has
impeded
broad
application
techniques.
Herein,
we
demonstrate
irreversible
engineering
three-dimensional
(3D)
(EC)
metal
(ECOMD)
This
approach
enables
organic
seed-programmable
growth
spontaneous
bottom-up
formation
simultaneously.
Because
net
charge
effect
participation
rate
lysates,
number
ECOMDs
increases
increasingly
positive
polarity.
lysates
embedded
function
as
both
dielectric
medium
field
confinement
an
analyte,
enabling
highly
specific
sensitive
(SLs)
at
concentrations
low
10
–2
plaque
forming
unit/mL.
ECOMD
used
trace
detect
SLs
human
saliva
diagnose
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2);
results
indicate
that
can
provide
point-of-care
diagnoses
infectious
diseases.
Journal of Nanobiotechnology,
Journal Year:
2023,
Volume and Issue:
21(1)
Published: May 6, 2023
Abstract
Surface-Enhanced
Raman
Scattering
(SERS)
technology,
as
a
powerful
tool
to
identify
molecular
species
by
collecting
spectral
signals
at
the
single-molecule
level,
has
achieved
substantial
progresses
in
fields
of
environmental
science,
medical
diagnosis,
food
safety,
and
biological
analysis.
As
deepening
research
is
delved
into
SERS
sensing,
more
high-performance
or
multifunctional
substrate
materials
emerge,
which
are
expected
push
sensing
application
fields.
Especially
field
analysis,
intrinsic
extrinsic
schemes
have
been
widely
used
explored
due
their
fast,
sensitive
reliable
advantages.
Herein,
recent
developments
substrates
applications
biomolecular
detection
(SARS-CoV-2
virus,
tumor
etc.),
imaging
pesticide
summarized.
The
concepts
(including
its
basic
theory
mechanism)
important
strategies
(extending
from
nanomaterials
with
tunable
shapes
nanostructures
surface
bio-functionalization
modifying
affinity
groups
specific
biomolecules)
for
improving
biosensing
performance
comprehensively
discussed.
For
data
analysis
identification,
machine
learning
methods
software
acquisition
sources
diagnosing
discussed
detail.
In
conclusion,
challenges
perspectives
future
presented.
Chemical Society Reviews,
Journal Year:
2023,
Volume and Issue:
52(24), P. 8500 - 8530
Published: Jan. 1, 2023
This
review
explores
various
point-of-care
optical
diagnostic
systems
combined
with
microdevices
developed
during
the
recent
COVID-19
pandemic
for
clinical
diagnostics.
ACS Sensors,
Journal Year:
2024,
Volume and Issue:
9(9), P. 4860 - 4869
Published: Sept. 5, 2024
Exosomes,
nanosized
extracellular
vesicles
containing
biomolecular
cargo,
are
increasingly
recognized
as
promising
noninvasive
biomarkers
for
cancer
diagnosis,
particularly
their
role
in
carrying
tumor-specific
molecular
information.
Traditional
methods
exosome
detection
face
challenges
such
complexity,
time
consumption,
and
the
need
sophisticated
equipment.
This
study
addresses
these
by
introducing
a
novel
droplet
microfluidic
platform
integrated
with
surface-enhanced
Raman
spectroscopy
(SERS)-based
aptasensor
rapid
sensitive
of
HER2-positive
exosomes
from
breast
cells.
Our
approach
utilized
an
on-chip
salt-induced
gold
nanoparticles
(GNPs)
aggregation
process
presence
HER2
aptamers
exosomes,
enhancing
hot
spot-based
SERS
signal
amplification.
achieved
limit
4.5
log
Nano Convergence,
Journal Year:
2024,
Volume and Issue:
11(1)
Published: April 30, 2024
This
review
reports
diverse
microfluidic
systems
utilizing
surface-enhanced
Raman
scattering
(SERS)
detection
for
disease
diagnosis.
Integrating
SERS
technology,
providing
high-sensitivity
detection,
and
technology
manipulating
small
liquid
samples
in
microdevices
has
expanded
the
analytical
capabilities
previously
confined
to
larger
settings.
study
explores
principles
uses
of
various
SERS-based
devices
developed
over
last
two
decades.
Specifically,
we
investigate
operational
documented
devices,
including
continuous-flow
channels,
microarray-embedded
droplet
digital
gradient
channels.
We
also
examine
their
applications
biomedical
diagnostics.
In
conclusion,
summarize
areas
requiring
further
development
translate
these
technologies
into
practical
clinical
