Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes

Cell, Journal Year: 2016, Volume and Issue: 165(7), P. 1789 - 1802

Published: May 28, 2016

Language: Английский

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Hydrogel machines

Materials Today, Journal Year: 2020, Volume and Issue: 36, P. 102 - 124

Published: Jan. 28, 2020

Language: Английский

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Haematopoietic stem cell activity and interactions with the niche

Nature Reviews Molecular Cell Biology, Journal Year: 2019, Volume and Issue: 20(5), P. 303 - 320

Published: Feb. 11, 2019

Language: Английский

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Shrinkage-mediated imaging of entire organs and organisms using uDISCO

Nature Methods, Journal Year: 2016, Volume and Issue: 13(10), P. 859 - 867

Published: Aug. 22, 2016

Language: Английский

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ScaleS: an optical clearing palette for biological imaging

Nature Neuroscience, Journal Year: 2015, Volume and Issue: 18(10), P. 1518 - 1529

Published: Sept. 14, 2015

Language: Английский

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Tissue clearing and its applications in neuroscience

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 21(2), P. 61 - 79

Published: Jan. 2, 2020

Language: Английский

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Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems

Cell, Journal Year: 2015, Volume and Issue: 163(6), P. 1500 - 1514

Published: Dec. 1, 2015

Language: Английский

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Genetic Dissection of Neural Circuits: A Decade of Progress

Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281

Published: April 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Language: Английский

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A protocol for combining fluorescent proteins with histological stains for diverse cell wall components

The Plant Journal, Journal Year: 2017, Volume and Issue: 93(2), P. 399 - 412

Published: Nov. 24, 2017

Higher plant function is contingent upon the complex three-dimensional (3D) architecture of tissues, yet severe light scattering renders deep, 3D tissue imaging very problematic. Although efforts to 'clear' tissues have been ongoing for over a century, many innovations made in recent years. Among them, protocol called ClearSee efficiently clears and diminishes chlorophyll autofluorescence while maintaining fluorescent proteins - thereby allowing analysis gene expression protein localisation cleared samples. To further increase usefulness this protocol, we developed ClearSee-based toolbox which number classical histological stains lignin, suberin other cell wall components can be used conjunction with reporter lines. We found that dyes are highly soluble solution, old staining protocols enormously simplified; these additionally unsuitable co-visualisation markers due harsh fixation clearing. Consecutive several allows distinct modifications as transcriptional reporters or tools deep within tissues. Moreover, easily applied on hand sections different organs. In combination confocal microscopy, improves image quality decreasing time cost embedding/sectioning. It thus provides low-cost, efficient method studying thick usually cumbersome visualise. Our ClearSee-adapted significantly improve speed up anatomical developmental investigations numerous species, hope they will contribute new discoveries areas research.

Language: Английский

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High-resolution 3D imaging of fixed and cleared organoids

Nature Protocols, Journal Year: 2019, Volume and Issue: 14(6), P. 1756 - 1771

Published: May 3, 2019

Language: Английский

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