An assay for chemical nociception inDrosophilalarvae DOI Creative Commons
Roger Lopez-Bellido, Nathaniel J. Himmel, Howard B. Gutstein

et al.

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2019, Volume and Issue: 374(1785), P. 20190282 - 20190282

Published: Sept. 23, 2019

Chemically induced nociception has not yet been studied intensively in genetically tractable models. Hence, our goal was to establish a Drosophila assay that can be used study the cellular and molecular/genetic bases of chemically nociception. larvae exposed increasing concentrations hydrochloric acid (HCl) produced an increasingly intense aversive rolling response. HCl (0.5%) subthreshold provoked no All classes peripheral multidendritic (md) sensory neurons (classes I–IV) are required for full responsiveness acid, with class IV making largest contribution. At level, IV, III I showed increases calcium following exposure. In central nervous system, Basin-4 second-order key regulators nociception, slight contribution from other types. Finally, chemical sensitized by tissue damage. Subthreshold allodynia 4 h after physical puncture wounding. Pinch wounding UV irradiation, which do compromise cuticle, did cause allodynia. sum, we developed novel larvae. This assay, combined high genetic resolving power Drosophila, should improve basic understanding fundamental mechanisms article is part Theo Murphy meeting issue ‘Evolution behaviour important pain’.

Language: Английский

Cellular and Neural Responses to Sour Stimuli Require the Proton Channel Otop1 DOI Creative Commons
Bochuan Teng,

Courtney Wilson,

Yu-Hsiang Tu

et al.

Current Biology, Journal Year: 2019, Volume and Issue: 29(21), P. 3647 - 3656.e5

Published: Sept. 19, 2019

The sense of taste allows animals to sample chemicals in the environment prior ingestion. Of five basic tastes, sour, acids, had remained among most mysterious. Acids are detected by type III receptor cells (TRCs), located buds across tongue and palate epithelium. first step sour transduction is believed be entry protons into cell cytosol, which leads cytosolic acidification generation action potentials. proton-selective ion channel Otop1 expressed TRCs a candidate receptor. Here, we tested contribution gustatory nerve responses acids mice was genetically inactivated (Otop1-KO mice). We show that required for inward proton current from different parts otherwise molecularly heterogeneous. next Otop1-KO mice, intracellular pH does not track with extracellular moderately acidic stimuli do elicit trains potentials, as they wild-type mice. Moreover, were severely selectively attenuated stimuli, including citric acid HCl. These results establish plays critical role detection mouse system, evidence it bona fide

Language: Английский

Citations

176
Recent advances in the genetic basis of taste detection in Drosophila DOI
Yu‐Chieh Chen, Anupama Dahanukar

Cellular and Molecular Life Sciences, Journal Year: 2019, Volume and Issue: 77(6), P. 1087 - 1101

Published: Oct. 9, 2019

Language: Английский

Citations

110
Drosophilasensory receptors—a set of molecular Swiss Army Knives DOI Open Access
Craig Montell

Genetics, Journal Year: 2021, Volume and Issue: 217(1), P. 1 - 34

Published: Jan. 1, 2021

Genetic approaches in the fruit fly, Drosophila melanogaster, have led to a major triumph field of sensory biology-the discovery multiple large families receptors and channels. Some these families, such as transient receptor potential channels, are conserved from animals ranging worms humans, while others, "gustatory receptors," "olfactory "ionotropic restricted invertebrates. Prior identification flies, it was widely assumed that proteins function just one modality vision, smell, taste, hearing, somatosensation, which includes thermosensation, light, noxious mechanical touch. By employing vast combination genetic, behavioral, electrophysiological, other concept emerge is many multitaskers. The earliest example this idea individual channels senses. It now clear multitasking exhibited by including gustatory receptors, ionotropic epithelial Na+ (also referred Pickpockets), even opsins, were formerly thought exclusively light sensors. characterizations neurons express them also reveal mechanisms through flies can accurately differentiate between different stimuli when they activate same receptor, well adaptation, amplification, integration. insights gleaned studies been highly influential directing investigations animal models.

Language: Английский

Citations

101
The Structure and Function of Ionotropic Receptors in Drosophila DOI Creative Commons
Lina Ni

Frontiers in Molecular Neuroscience, Journal Year: 2021, Volume and Issue: 13

Published: Feb. 1, 2021

Ionotropic receptors (IRs) are a highly divergent subfamily of ionotropic glutamate (iGluR) and conserved across Protostomia, major branch the animal kingdom that encompasses both Ecdysozoa Lophothrochozoa. They broadly expressed in peripheral sensory systems, concentrated dendrites, function chemosensation, thermosensation, hygrosensation. As iGluRs, four IR subunits form functional ion channel to detect environmental stimuli. Most comprise individual stimulus-specific tuning one or two coreceptors. This review summarizes discoveries structure complexes expression each IR, as well discusses future direction for studies.

Language: Английский

Citations

61
Molecular and neuronal mechanisms for amino acid taste perception in the Drosophila labellum DOI Creative Commons
Binod Aryal, Subash Dhakal, Bhanu Shrestha

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(6), P. 1376 - 1386.e4

Published: Feb. 16, 2022

Language: Английский

Citations

51
A molecular mechanism for high salt taste in Drosophila DOI Creative Commons
Sasha A.T. McDowell, Molly Stanley, Michael D. Gordon

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(14), P. 3070 - 3081.e5

Published: June 29, 2022

Language: Английский

Citations

42
Molecular sensors in the taste system of Drosophila DOI
Bhanu Shrestha, Youngseok Lee

Genes & Genomics, Journal Year: 2023, Volume and Issue: 45(6), P. 693 - 707

Published: Feb. 24, 2023

Language: Английский

Citations

23
Avoiding cantharidin through ionotropic receptors DOI
Roshani Nhuchhen Pradhan, Bhanu Shrestha, Youngseok Lee

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 466, P. 133497 - 133497

Published: Jan. 11, 2024

Language: Английский

Citations

11
Acetic acid activates distinct taste pathways in Drosophila to elicit opposing, state-dependent feeding responses DOI Creative Commons
Anita V. Devineni, Bei Sun, Anna Zhukovskaya

et al.

eLife, Journal Year: 2019, Volume and Issue: 8

Published: June 17, 2019

Taste circuits are genetically determined to elicit an innate appetitive or aversive response, ensuring that animals consume nutritious foods and avoid the ingestion of toxins. We have examined response Drosophila melanogaster acetic acid, a tastant can be metabolic resource but also toxic fly. Our data reveal flies accommodate these conflicting attributes acid by virtue hunger-dependent switch in their behavioral this stimulus. Fed show taste aversion whereas starved robust response. These opposing responses mediated two different classes neurons, sugar- bitter-sensing neurons. Hunger shifts from attraction enhancing sugar pathway as well suppressing bitter pathway. Thus single drive behaviors activating distinct pathways modulated internal state.

Language: Английский

Citations

67
Mechanisms of lactic acid gustatory attraction in Drosophila DOI Creative Commons
Molly Stanley, Britya Ghosh, Zachary F. Weiss

et al.

Current Biology, Journal Year: 2021, Volume and Issue: 31(16), P. 3525 - 3537.e6

Published: June 30, 2021

Language: Английский

Citations

55