Cholesterol taste avoidance in Drosophila melanogaster DOI Creative Commons
Roshani Nhuchhen Pradhan, Craig Montell, Youngseok Lee

et al.

eLife, Journal Year: 2025, Volume and Issue: 14

Published: April 17, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster , capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. cholesterol-responsive GRNs comprise a subset also responds bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies' ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

Context-dependent reversal of odorant preference is driven by inversion of the response in a single sensory neuron type DOI Creative Commons

Munzareen Khan,

Anna H. Hartmann,

Michael P. O’Donnell

et al.

PLoS Biology, Journal Year: 2022, Volume and Issue: 20(6), P. e3001677 - e3001677

Published: June 13, 2022

The valence and salience of individual odorants are modulated by an animal’s innate preferences, learned associations, internal state, as well the context odorant presentation. mechanisms underlying context-dependent flexibility in odor not fully understood. Here, we show that behavioral response Caenorhabditis elegans to bacterially produced medium-chain alcohols switches from attraction avoidance when presented background a subset additional attractive chemicals. This reversal preference is driven cell-autonomous inversion these single AWC olfactory neuron pair. We find while inhibit neurons drive attraction, instead activate promote second AWC-sensed odorant. opposing responses via engagement distinct odorant-directed signal transduction pathways within AWC. Our results indicate recruitment alternative intracellular signaling sensory type conveys opposite hedonic valences, thereby providing robust mechanism for encoding discrimination at periphery.

Language: Английский

Citations

65
A molecular mechanism for high salt taste in Drosophila DOI Creative Commons
Sasha A.T. McDowell, Molly Stanley, Michael D. Gordon

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(14), P. 3070 - 3081.e5

Published: June 29, 2022

Language: Английский

Citations

42
Molecular sensors in the taste system of Drosophila DOI
Bhanu Shrestha, Youngseok Lee

Genes & Genomics, Journal Year: 2023, Volume and Issue: 45(6), P. 693 - 707

Published: Feb. 24, 2023

Language: Английский

Citations

23
Molecular and Cellular Mechanisms of Salt Taste DOI
Akiyuki Taruno, Michael D. Gordon

Annual Review of Physiology, Journal Year: 2022, Volume and Issue: 85(1), P. 25 - 45

Published: Nov. 5, 2022

Salt taste, the taste of sodium chloride (NaCl), is mechanistically one most complex and puzzling among basic tastes. Sodium has essential functions in body but causes harm excess. Thus, animals use salt to ingest right amount salt, which fluctuates by physiological needs: typically, attraction low concentrations rejection high salt. This concentration-valence relationship universally observed terrestrial animals, research revealed peripheral codes for NaCl involving multiple pathways opposing valence. Sodium-dependent -independent mediate aversion NaCl, respectively. Gustatory sensors cells that transduce have been uncovered, along with downstream signal transduction neurotransmission mechanisms. However, much remains unknown. article reviews classical recent advances our understanding molecular cellular mechanisms underlying mammals insects discusses perspectives on human taste.

Language: Английский

Citations

36
Meeting a threat of the Anthropocene: Taste avoidance of metal ions by Drosophila DOI Creative Commons
Shuke Xiao, Lisa Soyeon Baik,

Xueying Shang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(25)

Published: June 14, 2022

The Anthropocene Epoch poses a critical challenge for organisms: they must cope with new threats at rapid rate. These include toxic chemical compounds released into the environment by human activities. Here, we examine elevated concentrations of heavy metal ions as an example anthropogenic stressors. We find that fruit fly Drosophila avoids nine when present flies experienced rarely, if ever, until Anthropocene. characterize avoidance feeding and egg laying on ions, identify receptors, neurons, taste organs contribute to this avoidance. Different subsets including members both Ir (Ionotropic receptor) Gr (Gustatory families different ions. activate certain bitter-sensing neurons inhibit sugar-sensing neurons. Some behavioral responses are mediated largely through pharynx. Feeding remains stable over 10 generations exposure copper zinc conserved across diverse dipteran species, mosquito Aedes albopictus . Our results suggest mechanisms may be essential insects face challenges from environmental changes in

Language: Английский

Citations

35
Complex representation of taste quality by second-order gustatory neurons in Drosophila DOI Creative Commons
Nathaniel J. Snell, Jeremy Fisher, Griffin G. Hartmann

et al.

Current Biology, Journal Year: 2022, Volume and Issue: 32(17), P. 3758 - 3772.e4

Published: Aug. 15, 2022

Language: Английский

Citations

32
Taste coding of heavy metal ion-induced avoidance in Drosophila DOI Creative Commons
Xiaonan Li, Yuanjie Sun, Shan Gao

et al.

iScience, Journal Year: 2023, Volume and Issue: 26(5), P. 106607 - 106607

Published: April 7, 2023

Increasing pollution of heavy metals poses great risks to animals globally. Their survival likely relies on an ability detect and avoid harmful metal ions (HMIs). Currently, little is known about the neural mechanisms HMI detection. Here, we show that Drosophila related species Drosophilidae actively toxic HMIs at micromolar concentrations. The high sensitivity biologically relevant. Particularly, their cadmium as most bitter substance, denatonium. Detection in food requires Gr66a+ gustatory neurons but independent bitter-taste receptors. In these neurons, ionotropic receptors IR76b, IR25a, IR7a are required for perception metals. Furthermore, IR47a mediates activation a distinct group non-Gr66a+ elicited by HMIs. Together, our findings reveal surprising taste quality represented noxious ions.

Language: Английский

Citations

20
Molecular Basis of Hexanoic Acid Taste in Drosophila melanogaster DOI Open Access
Roshani Nhuchhen Pradhan, Bhanu Shrestha, Youngseok Lee

et al.

Molecules and Cells, Journal Year: 2023, Volume and Issue: 46(7), P. 451 - 460

Published: May 19, 2023

Animals generally prefer nutrients and avoid toxic harmful chemicals.Recent behavioral physiological studies have identified that sweet-sensing gustatory receptor neurons (GRNs) in Drosophila melanogaster mediate appetitive behaviors toward fatty acids.Sweet-sensing GRN activation requires the function of ionotropic receptors IR25a, IR56d, IR76b, as well GR64e.However, we reveal hexanoic acid (HA) is rather than nutritious to D. melanogaster.HA one major components fruit Morinda citrifolia (noni).Thus, analyzed responses noni acids, HA, via electrophysiology proboscis extension response (PER) assay.Electrophysiological tests show this reminiscent arginine-mediated neuronal responses.Here, determined a low concentration HA induced attraction, which was mediated by GRNs, high aversion, bitter-sensing GRNs.We also demonstrated elicits attraction mainly GR64d IR56d expressed but activates three (GR32a, GR33a, GR66a) GRNs.The mechanism sensing biphasic dose dependent manner.Furthermore, inhibit sugar-mediated like other bitter compounds.Taken together, discovered binary HA-sensing may be evolutionarily meaningful foraging niche insects.

Language: Английский

Citations

20
A single pair of pharyngeal neurons functions as a commander to reject high salt in Drosophila melanogaster DOI Creative Commons
Jiun Sang, Subash Dhakal, Bhanu Shrestha

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 27, 2023

Salt (NaCl), is an essential nutrient for survival, while excessive salt can be detrimental. In the fruit fly, Drosophila melanogaster , internal taste organs in pharynx are critical gatekeepers impacting decision to accept or reject a food. Currently, our understanding of mechanism through which pharyngeal gustatory receptor neurons (GRNs) sense high rudimentary. Here, we found that member ionotropic family, Ir60b expressed exclusively pair GRNs activated by salt. Using two-way choice assay (DrosoX) measure ingestion volume, demonstrate IR60b and two co-receptors IR25a IR76b required prevent consumption. Mutants lacking external but retaining exhibit much higher avoidance than flies with all missing three IRs. Our findings highlight vital role IRs GRN control

Language: Английский

Citations

17
Requirement for an Otopetrin-like protein for acid taste in Drosophila DOI Creative Commons
Anindya Ganguly, Avinash Chandel, Heather N. Turner

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(51)

Published: Dec. 15, 2021

Significance The taste of acids is critical for animal survival since it enables them to differentiate potentially dangerous from nutritious foods. Due the general requirement acid survival, we tested idea that receptor mechanism functioning in may be evolutionarily conserved. Here, demonstrate mutation a Drosophila gene, Otopetrin-Like A ( OtopLA ), encoding protein distantly related recently identified mammalian receptor, OTOP1, essential both strong repulsion highly acidic food and mild attraction low acidity. aversion requires expression distinct neurons fly equivalent vertebrate tongue.

Language: Английский

Citations

34