A single-dose of intranasal vaccination with a live-attenuated SARS-CoV-2 vaccine candidate promotes protective mucosal and systemic immunity DOI Open Access

Awadalkareem Adam,

Birte Kalveram,

John Yun-Chung Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 18, 2023

An attenuated SARS-CoV-2 virus with modified viral transcriptional regulatory sequences and deletion of open-reading frames 3, 6, 7 8 (∆3678) was previously reported to protect hamsters from infection transmission. Here we report that a single-dose intranasal vaccination ∆3678 protects K18-hACE2 mice wild-type or variant challenge. Compared infection, the induces equivalent higher levels lung systemic T cell, B IgA, IgG responses. The results suggest as an attractive mucosal vaccine candidate boost pulmonary immunity against SARS-CoV-2.

Language: Английский

Microbiota and Immunity during Respiratory Infections: Lung and Gut Affair DOI Open Access
Veronica Marrella,

Federico Nicchiotti,

Barbara Cassani

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4051 - 4051

Published: April 5, 2024

Bacterial and viral respiratory tract infections are the most common infectious diseases, leading to worldwide morbidity mortality. In past 10 years, importance of lung microbiota emerged in context pulmonary although mechanisms by which it impacts intestinal environment have not yet been fully identified. On contrary, gut microbial dysbiosis is associated with disease etiology or/and development lung. this review, we present an overview microbiome modifications occurring during infections, namely, reduced community diversity increased burden, downstream consequences on host–pathogen interaction, inflammatory signals, cytokines production, turn affecting progression outcome. Particularly, focus role gut–lung bidirectional communication shaping inflammation immunity context, resuming both animal human studies. Moreover, discuss challenges possibilities related novel microbial-based (probiotics dietary supplementation) microbial-targeted therapies (antibacterial monoclonal antibodies bacteriophages), aimed remodel composition resident communities restore health. Finally, propose outlook some relevant questions field be answered future research, may translational relevance for prevention control infections.

Language: Английский

Citations

18
Immune Response to Respiratory Viral Infections DOI Open Access
Antonella Gambadauro, Francesca Galletta, Alessandra Li Pomi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6178 - 6178

Published: June 4, 2024

The respiratory system is constantly exposed to viral infections that are responsible for mild severe diseases. In this narrative review, we focalized the attention on syncytial virus (RSV), influenza virus, and acute syndrome-coronavirus-2 (SARS-CoV-2) infections, high morbidity mortality in last decades. We reviewed human innate adaptive immune responses airways following infection, focusing a particular population: newborns pregnant women. recent Coronavirus disease-2019 (COVID-19) pandemic has highlighted how our interest pathologies must not decrease. Furthermore, increase knowledge of infection mechanisms improve future defense strategies.

Language: Английский

Citations

18
Tissue‐resident memory T cells and lung immunopathology DOI Creative Commons
In Su Cheon, Young Min Son, Jie Sun

et al.

Immunological Reviews, Journal Year: 2023, Volume and Issue: 316(1), P. 63 - 83

Published: April 4, 2023

Rapid reaction to microbes invading mucosal tissues is key protect the host against disease. Respiratory tissue-resident memory T (T

Language: Английский

Citations

34
Three SARS-CoV-2 spike protein variants delivered intranasally by measles and mumps vaccines are broadly protective DOI Creative Commons

Yuexiu Zhang,

Michelle Chamblee,

Jiayu Xu

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: July 3, 2024

Abstract As the new SARS-CoV-2 Omicron variants and subvariants emerge, there is an urgency to develop intranasal, broadly protective vaccines. Here, we developed highly efficacious, intranasal trivalent vaccine candidates (TVC) based on three components of MMR vaccine: measles virus (MeV), mumps (MuV) Jeryl Lynn (JL1) strain, MuV JL2 strain. Specifically, MeV, MuV-JL1, MuV-JL2 strains, each expressing prefusion spike (preS-6P) from a different variant concern (VoC), were combined generate TVCs. Intranasal immunization IFNAR1 −/− mice female hamsters with TVCs generated high levels S-specific serum IgG antibodies, broad neutralizing mucosal IgA antibodies as well tissue-resident memory T cells in lungs. The immunized protected challenge original WA1, B.1.617.2, B.1.1.529 strains. preexisting MeV immunity does not significantly interfere efficacy TVC. Thus, platform promising next-generation candidate.

Language: Английский

Citations

6
Systemic prime mucosal boost significantly increases protective efficacy of bivalent RSV influenza viral vectored vaccine DOI Creative Commons
Cameron Bissett, Sandra Belij‐Rammerstorfer, Marta Ulaszewska

et al.

npj Vaccines, Journal Year: 2024, Volume and Issue: 9(1)

Published: June 26, 2024

Abstract Although licensed vaccines against influenza virus have been successful in reducing pathogen-mediated disease, they less effective at preventing viral infection of the airways and current seasonal updates to do not always successfully accommodate drift. Most recently RSV are administered via intramuscular route. Alternative immunisation strategies, such as intranasal vaccinations, “prime-pull” regimens, may deliver a more sterilising form protection respiratory viruses. A bivalent ChAdOx1-based vaccine (ChAdOx1-NP + M1-RSVF) encoding conserved nucleoprotein matrix 1 proteins from modified pre-fusion stabilised F protein, was designed, developed tested preclinical animal models. The aim induce broad, cross-protective tissue-resident T cells heterotypic viruses neutralising antibodies mucosa systemically. When an prime-intranasal boost (IM-IN) regimen mice, superior generated challenge with either A, Influenza H3N2 or H1N1. These results support further clinical development pan & prime-pull regimen.

Language: Английский

Citations

4
Viral-vectored respiratory mucosal vaccine strategies DOI

Mangalakumari Jeyanathan,

Sam Afkhami,

Alisha Kang

et al.

Current Opinion in Immunology, Journal Year: 2023, Volume and Issue: 84, P. 102370 - 102370

Published: July 25, 2023

Language: Английский

Citations

8
Organoid modeling of lung-resident immune responses to SARS-CoV-2 infection DOI Creative Commons
Calvin J. Kuo, S. S. Choi, Vincent van Unen

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 5, 2023

Abstract Tissue-resident immunity underlies essential host defenses against pathogens, but analysis in humans has lacked vitro model systems where epithelial infection and accompanying resident immune cell responses can be observed en bloc. Indeed, human primary organoid cultures typically omit cells, tissue resident-memory lymphocytes are conventionally assayed without an component, for instance from peripheral blood, or after extraction organs. Further, the study of animals complicated by interchange between compartments. To tissue-resident infectious isolation secondary lymphoid organs, we generated adult lung three-dimensional air-liquid interface (ALI) organoids intact fragments that co-preserve stromal architecture alongside endogenous lung-resident subsets. These included CD69 + CD103 CCR7- and/or CD45RA - TRM, B, NK myeloid with conservation T receptor repertoires, all corresponding to matched fresh tissue. SARS-CoV-2 vigorously infected epithelium, induction innate cytokine production was inhibited antiviral agents. Notably, SARS-CoV-2-infected manifested adaptive virus-specific activation specific seropositive previously donor individuals. This holistic non-reconstitutive system demonstrates sufficiency autonomously mount memory a represents enabling method immunity.

Language: Английский

Citations

7
Development of T cell antigen-based human coronavirus vaccines against nAb-escaping SARS-CoV-2 variants DOI
Hao Zhou, Ping Leng, Yang Wang

et al.

Science Bulletin, Journal Year: 2024, Volume and Issue: 69(15), P. 2456 - 2470

Published: June 12, 2024

Language: Английский

Citations

2
Regional specialization within the mammalian respiratory immune system DOI
David P. Hoytema van Konijnenburg,

Peter A. Nigrovic,

Ivan Zanoni

et al.

Trends in Immunology, Journal Year: 2024, Volume and Issue: 45(11), P. 871 - 891

Published: Oct. 21, 2024

Language: Английский

Citations

2
Triphasic production of IFNγ by innate and adaptive lymphocytes following influenza A virus infection DOI Creative Commons
George Finney, Kerrie E Hargrave, Marieke Pingen

et al.

Discovery Immunology, Journal Year: 2023, Volume and Issue: 2(1)

Published: Jan. 1, 2023

Abstract Interferon gamma (IFNγ) is a potent antiviral cytokine that can be produced by many innate and adaptive immune cells during infection. Currently, our understanding of which produce IFNγ where they are located at different stages an infection limited. We have used reporter mice to investigate in vivo expression Ifnγ mRNA the lung secondary lymphoid organs following influenza A virus (IAV) observed triphasic production expression. Unconventional T cells, particularly NK were dominant producers early Ifnγ, while CD4 CD8 main day 10 post-infection. Following viral clearance, some memory continued express lungs draining lymph node. Interestingly, node natural killer (NK), NKT, type 1 also above naïve levels, suggesting memory-like phenotypes for these cells. Analysis localization Ifnγ+ demonstrated cytokine+ near airways parenchyma. second IAV challenge, IAV-specific rapidly increased their response. Together, data suggest fluctuates based on cellular source location, both could impact subsequent responses.

Language: Английский

Citations

6