Animals
use
acoustic
signals
across
a
variety
of
social
behaviors,
particularly
courtship.
In
Drosophila,
song
is
detected
by
antennal
mechanosensory
neurons
and
further
processed
second-order
aPN1/aLN(al)
neurons.
However,
little
known
about
the
central
pathways
mediating
courtship
hearing.
this
study,
we
identified
male-specific
pathway
for
hearing
via
third-order
ventrolateral
protocerebrum
Projection
Neuron
1
(vPN1)
fourth-order
pC1
Genetic
inactivation
vPN1
or
disrupts
song-induced
male-chaining
behavior.
Calcium
imaging
reveals
that
responds
preferentially
to
pulse
with
long
inter-pulse
intervals
(IPIs),
while
responses
closely
match
behavioral
chaining
at
different
IPIs.
Moreover,
genetic
activation
either
induced
chaining,
mimicking
response
song.
These
results
outline
aPN1-vPN1-pC1
as
labeled
line
processing
transformation
in
males.
Cell,
Journal Year:
2018,
Volume and Issue:
174(3), P. 730 - 743.e22
Published: July 1, 2018
Drosophila
melanogaster
has
a
rich
repertoire
of
innate
and
learned
behaviors.
Its
100,000-neuron
brain
is
large
but
tractable
target
for
comprehensive
neural
circuit
mapping.
Only
electron
microscopy
(EM)
enables
complete,
unbiased
mapping
synaptic
connectivity;
however,
the
fly
too
conventional
EM.
We
developed
custom
high-throughput
EM
platform
imaged
entire
an
adult
female
at
resolution.
To
validate
dataset,
we
traced
brain-spanning
circuitry
involving
mushroom
body
(MB),
which
been
extensively
studied
its
role
in
learning.
All
inputs
to
Kenyon
cells
(KCs),
intrinsic
neurons
MB,
were
mapped,
revealing
previously
unknown
cell
type,
postsynaptic
partners
KC
dendrites,
unexpected
clustering
olfactory
projection
neurons.
These
reconstructions
show
that
this
freely
available
volume
supports
circuits,
will
significantly
accelerate
neuroscience.
VIDEO
ABSTRACT.
Nature,
Journal Year:
2024,
Volume and Issue:
630(8017), P. 686 - 694
Published: June 5, 2024
Abstract
To
convert
intentions
into
actions,
movement
instructions
must
pass
from
the
brain
to
downstream
motor
circuits
through
descending
neurons
(DNs).
These
include
small
sets
of
command-like
that
are
sufficient
drive
behaviours
1
—the
circuit
mechanisms
for
which
remain
unclear.
Here
we
show
DNs
in
Drosophila
directly
recruit
networks
additional
orchestrate
require
active
control
numerous
body
parts.
Specifically,
found
previously
thought
alone
2–4
fact
co-activate
larger
populations
DNs.
Connectome
analyses
and
experimental
manipulations
revealed
this
functional
recruitment
can
be
explained
by
direct
excitatory
connections
between
interconnected
brain.
Descending
population
is
necessary
behavioural
control:
with
many
partners
network
co-activation
complete
only
simple
stereotyped
movements
their
absence.
DN
reside
within
behaviour-specific
clusters
inhibit
one
another.
results
support
a
mechanism
generated
increasingly
large
compose
combining
multiple
subroutines.
Journal of Experimental Biology,
Journal Year:
2017,
Volume and Issue:
220(1), P. 25 - 34
Published: Jan. 1, 2017
ABSTRACT
Recent
developments
in
machine
vision
methods
for
automatic,
quantitative
analysis
of
social
behavior
have
immensely
improved
both
the
scale
and
level
resolution
with
which
we
can
dissect
interactions
between
members
same
species.
In
this
paper,
review
these
methods,
a
particular
focus
on
how
biologists
apply
them
to
their
own
work.
We
discuss
several
components
vision-based
analyses:
record
high-quality
video
automated
analyses,
video-based
tracking
algorithms
estimating
positions
interacting
animals,
learning
recognizing
patterns
interactions.
These
are
extremely
general
applicability,
subset
successful
applications
biological
questions
model
systems
very
different
types
behaviors.
