Cited by Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing

Effect of eicosapentaenoic acid on innate immune responses in Atlantic salmon cells infected with infectious salmon anemia virus DOI

Ingrid Holmlund,

Samira Ahmadi,

Bente Ruyter

et al.

Virology Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: Jan. 9, 2025

Abstract Aquaculture is one of the world's fastest-growing sectors in food production but with multiple challenges related to animal handling and infections. The disease caused by infectious salmon anemia virus (ISAV) leads outbreaks local epidemics, reducing welfare, causing significant economic losses. composition feed has shifted from marine ingredients such as fish oil meal towards a more plant-based diet reduced levels eicosapentaenoic acid (EPA). aim this study was investigate whether low or high EPA affect expression genes innate immune response 48 h after infection ISAV. includes seven experimental groups: ± ISAV various up 200 µM. Analysis RNA sequencing data showed that than 3000 were affected alone (without additional EPA). In cells increasing EPA, 2500 differentially expressed. This indicates concentration have an independent effect on gene virus-infected cells, not observed at lower EPA. Analyses enriched biological processes molecular functions (GO KEGG analysis) revealed had limited impact system alone, many when infected. Several pathways affected, including protein synthesis (ribosomal transcripts), peroxisome proliferator activated receptor (PPAR) signaling, ferroptosis. Cells exposed both concentrations displayed patterns indicating increased formation oxygen radicals cell death via ferroptosis activated. pattern during Atlantic kidney (ASK) highest level (200 μM) without infection. Cell may therefore be mechanism for controlled thus reduction replication there are enough polyunsaturated fatty acids (PUFAs) membrane.

Language: Английский

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Singapore grouper iridovirus infection counteracts poly I:C induced antiviral immune response in vitro DOI

Wenji Wang, Ya Zhang,

Xixi Guo

et al.

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 135, P. 108685 - 108685

Published: March 13, 2023

Language: Английский

Citations

Functional Characterization of the Cnidarian Antiviral Immune Response Reveals Ancestral Complexity DOI

Magda Lewandowska,

Ton Sharoni,

Yael Admoni

et al.

Molecular Biology and Evolution, Journal Year: 2021, Volume and Issue: 38(10), P. 4546 - 4561

Published: June 26, 2021

Abstract Animals evolved a broad repertoire of innate immune sensors and downstream effector cascades for defense against RNA viruses. Yet, this system varies greatly among different bilaterian animals, masking its ancestral state. In study, we aimed to characterize the antiviral response cnidarian Nematostella vectensis decipher function retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) known detect viral double-stranded (dsRNA) in bilaterians but activate pathways vertebrates nematodes. We show that polyinosinic:polycytidylic acid (poly(I:C)), mimic long dsRNA primary ligand vertebrate RLR melanoma differentiation-associated protein 5 (MDA5), triggers complex bearing features distinctive both invertebrate systems. Importantly, well-characterized agonist RIG-I receptor does not induce significant transcriptomic bears signature response, which experimentally supports results phylogenetic analysis indicating clustering two N. paralogs (NveRLRa NveRLRb) with MDA5. Furthermore, affinity assays reveal NveRLRb binds poly(I:C) knockdown impairs expression putative genes including interference components. Our study provides first time functional evidence conserved role RLRs initiating originated before cnidarian–bilaterian split lay strong foundation future research on evolution responses

Language: Английский

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Overexpression of miR‐155 Modulates Interferon Response and Inhibits Viral Replication of IHNV and IPNV in EPC Cells DOI

Najib Abdellaoui,

I W Park,

Subin Choi

et al.

Journal of Fish Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

ABSTRACT Infections caused by RNA viruses, including infectious haematopoietic necrosis virus (IHNV) and pancreatic (IPNV), result in substantial economic losses the aquaculture industry. MicroRNAs (miRNAs), particularly miR‐155, play crucial roles regulating host immune responses viral infections. In this study, we investigated overexpression effect of miR‐155 Epithelioma papulosum cyprini (EPC) cells infected with IHNV IPNV analysed mechanisms underlying these antiviral activities. EPC were transfected at 40 pmol then or an MOI 0.01. The cytopathogenic (CPE) was observed for 5 days post‐infection. did not show any signs CPE exhibit growth over time after infection both viruses. Additionally, real‐time qPCR type I interferon‐related genes ( ISG15 Mx1 ) showed upregulation 0, 24, 48 h.p.i miR‐155. At h post‐infection, bands protein western blot. Furthermore, significantly enhanced expression interferon response targeting BCL2 CYLD suppressed replication through directly genes, L gene VP2 IPNV. These findings elucidate dual mechanism miR‐155's modulation direct targeting, offering insights developing novel strategies aquaculture.

Language: Английский

Citations

Infectious Salmon Anemia: The Current State of Knowledge Regarding ISAV DOI

Christopher J. Engert, Lucia Belen Armas Gastulo, Jefferson Yunis‐Aguinaga

et al.

Reviews in Fisheries Science & Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 28

Published: Feb. 18, 2025

Infectious Salmon Anemia (ISA), first reported in Norway 1984, is caused by Isavirus salaris (ISAV), which has two phenotypes: pathogenic (HPRΔ), causing high mortality farmed Atlantic salmon and significant economic losses, nonpathogenic (HPR0), widespread but non-lethal. However, HPR0 poses a persistent threat due to its potential mutate into forms, complicating detection, study, containment. This challenge exacerbated presence farming sites, hatcheries, wild fish populations. Effective control relies on strict regulatory policies, while future strategies focus developing biotechnological vaccines genetically resistant through selective breeding. review synthesizes peer-reviewed literature governmental data evaluate the global status of ISAV impact industry.

Language: Английский

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A synergistic blend of dietary organic acids, monoglycerides and phytobiotics enhance growth performance, intestinal mucosal height, and anti-viral immune gene expression in juvenile Barramundi (Lates calcarifer) DOI

Chern-Sing Goh,

Susan Gibson‐Kueh,

David Bal

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 41, P. 102692 - 102692

Published: Feb. 21, 2025

Language: Английский

Citations

Understanding host response to infectious salmon anaemia virus in an Atlantic salmon cell line using single-cell RNA sequencing DOI

Ophélie Gervais, Carolina Peñaloza, Remi L. Gratacap

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: March 29, 2023

Infectious Salmon Anaemia Virus (ISAV) is an Orthomixovirus that represents a large problem for salmonid aquaculture worldwide. Current prevention and treatment methods are only partially effective. Genetic selection genome engineering have the potential to develop ISAV resistant salmon stocks. Both strategies can benefit from improved understanding of genomic regulation pathogenesis. Here, we used single-cell RNA sequencing Atlantic cell line provide first high dimensional insight into transcriptional landscape underpins host-virus interaction during early infection.Salmon head kidney (SHK-1) cells were sequenced at 24, 48 96 h post-ISAV challenge. At 24 post infection, showed expression signatures consistent with viral entry, genes such as PI3K, FAK or JNK being upregulated relative uninfected cells. h, infected clear anti-viral response, characterised by IFNA2 IRF2. Uninfected bystander also differences, potentially suggesting paracrine signalling These expressed pathways mRNA sensing, degradation, ubiquitination proteasome; up-regulation mitochondrial ribosome seemed play role in host response infection. Correlation between revealed novel key this fish-virus interaction.This study has increased our cellular infection interactions level. Our results highlight various interaction, which be manipulated future functional studies increase resistance ISAV.

Language: Английский

Citations

Transcriptomics of early responses to purified Piscine orthoreovirus-1 in Atlantic salmon (Salmo salar L.) red blood cells compared to non-susceptible cell lines DOI

Thomais Tsoulia, Arvind Y. M. Sundaram,

Stine Braaen

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 14, 2024

Piscine red blood cells (RBC) are nucleated and have been characterized as mediators of immune responses in addition to their role gas exchange. Salmonid RBC major target orthoreovirus-1 (PRV-1), the etiological agent heart skeletal muscle inflammation (HSMI) farmed Atlantic salmon (Salmo salar). PRV-1 replicates ex vivo, but no viral amplification has possible available A. cell lines. To compare basal transcripts transcriptional early phase infection with non-susceptible cells, we exposed RBC, kidney (ASK) Salmon head (SHK-1) for 24 h. The RNA-seq analysis supported previous characterization pluripotent they expressed a wide repertoire genes encoding pattern recognition receptors (PRRs), cytokine receptors, implicated antiviral activities. comparison ASK SHK-1 revealed features exclusively such involved chemotactic activity response inflammation. Differential expression showed 46 significantly induced (≥ 2-fold upregulation) linked pathway, including RNA-specific PRRs interferon (IFN) factors. In SHK-1, PRV more potent or faster (213 induced). differential (12 induced, 18 suppressed) less by dsRNA-induced pathway. Despite these differences, RIG-I-like receptor 3 (RLR3) family cytosolic dsRNA was all cells. IFN regulatory factor 1 (IRF1) only, contrast IRF3/IRF7 SHK-1. Differences IRF may potentially affect propagation.

Language: Английский

Citations

Exploring genetic resistance to infectious salmon anaemia virus in Atlantic salmon by genome-wide association and RNA sequencing DOI

Ophélie Gervais, Agustín Barría, A. Papadopoulou

et al.

BMC Genomics, Journal Year: 2021, Volume and Issue: 22(1)

Published: May 13, 2021

Abstract Background Infectious Salmonid Anaemia Virus (ISAV) causes a notifiable disease that poses large threat for Atlantic salmon ( Salmo salar ) aquaculture worldwide. There is no fully effective treatment or vaccine, and therefore selective breeding to increase resistance ISAV promising avenue prevention. Genomic selection potentially genome editing can be applied enhance host resistance, these approaches benefit from improved knowledge of the genetic functional basis target trait. The aim this study was characterise architecture in commercial population its underlying genomic using RNA Sequencing. Results A total 2833 parr belonging 194 families were exposed cohabitation challenge which cumulative mortality reached 63% over 55 days. 1353 animals genotyped K SNP array, estimate heritability trait binary survival 0.13–0.33 (pedigree-genomic). genome-wide association analysis confirmed polygenic trait, albeit region chromosome Ssa13 significantly associated with explained 3% variance. sequencing heart 16 infected (7 14 days post infection) 8 control fish highlighted 4927 2437 differentially expressed genes at 7 infection respectively. complement coagulation pathway down-regulated fish, while several metabolic pathways up-regulated. interferon showed little evidence up-regulation but mildly activated days, suggesting potential crosstalk between virus. Comparison transcriptomic response high low values TRIM25 as being up-regulated resistant fish. Conclusions shows moderate architecture, significant QTL detected on 13. mild characterises virus samples salmon, candidate showing differential expression identified.

Language: Английский

Citations

Effects of eicosapentaneoic acid on innate immune responses in an Atlantic salmon kidney cell line in vitro DOI

Tor Gjøen,

Bente Ruyter,

Tone‐Kari Østbye

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0302286 - e0302286

Published: May 28, 2024

Studies of the interplay between metabolism and immunity, known as immunometabolism, is steadily transforming immunological research into new understandings how environmental cues like diet are affecting innate adaptive immune responses. The aim this study was to explore antiviral transcriptomic responses under various levels polyunsaturated fatty acid. Atlantic salmon kidney cells (ASK cell line) were incubated for one week in different unsaturated n-3 eicosapentaneoic acid (EPA) resulting cellular ranging from 2–20% total These then stimulated with viral mimic interferon inducer poly I:C (30 ug/ml) 24 hours before RNA isolated sequenced analyses. Up 200 uM EPA had no detrimental effects on viability induced very few transcriptional changes these cells. However, combination I:C, our results shows that level membranes exert profound dose dependent profiles by treatment. Metabolic pathways autophagy, apelin VEGF signaling attenuated whereas transcripts related metabolism, ferroptosis PPAR upregulated. suggests heavily influenced profile salmonid constitute another example strong linkage general metabolic inflammatory

Language: Английский

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