Journal of Ethnopharmacology,
Journal Year:
2024,
Volume and Issue:
326, P. 117901 - 117901
Published: Feb. 8, 2024
Wuji
Wan
(WJW)
is
a
traditional
Chinese
medicine
formula
that
can
be
found
in
the
"Prescriptions
of
Taiping
Benevolent
Dispensary"
has
been
employed
treating
gastric
discomfort,
burning
epigastric
pain,
and
reflux
for
hundreds
years
shown
promise
ulcers
(GUs).
However,
active
components
mechanism
action
against
GUs
remain
unclear.
The
aim
this
study
was
to
explore
WJW
elucidate
underlying
involved
GUs.
Initially,
cell
viability
measured
by
counting
kit
8
(CCK-8)
assay
evaluate
efficacy
WJW-containing
serum
vitro.
ulcer
index,
inhibition
rate,
hematoxylin
staining
(H&E),
periodic
acid-Schiff
(PAS)
were
used
therapeutic
effect
vivo.
Subsequently,
levels
inflammatory
factors
oxidative
stress
determined
using
an
enzyme-linked
immunosorbent
assays
(ELISA)
on
vitro
vivo
samples.
Additionally,
UPLC-Q
Exactive
Plus
Orbitrap
HRMS
analyze
absorbed
into
blood
its
metabolites.
Network
pharmacology
metabolomics
subsequently
identify
targets
pathways.
Real-time
quantitative
PCR
(RT‒qPCR)
Western
blotting
verify
mRNA
protein
key
Finally,
identified
molecular
docking
binding
stability
targets.
ameliorated
ethanol-induced
damage
GES-1
cells
promoted
healing.
reduced
IL-6,
TNF-α,
MDA,
LDH
while
increasing
IL-10,
SOD,
T-AOC
cells.
Moreover,
treatment
resulted
decreased
MDA
increased
PGE2,
NO
rats.
In
addition,
eight
blood.
network
results
revealed
192
common
entry
GUs,
KEGG
analysis
apoptosis
signaling
pathways
main
activity
Metabolomic
screening
13
differential
There
23
components,
metabolites,
with
TNF
(TNF-α),
AKT1,
PTGS2
(COX2)
MAPK1.
significantly
inhibited
expression
Bax,
Caspase-9,
Caspase-3,
cleaved
COX2,
p-p44/42
MAPK
promoting
Bcl-2
p-AKT1.
Molecular
are
berberine,
palmatine,
coptisine,
evodiamine,
rutaecarpine,
evocarpine,
paeoniflorin.
reduces
inflammation
injury
inhibits
paper,
we
provide
new
strategy
exploring
formulas
diseases
based
target
mechanisms.
Biomedicine & Pharmacotherapy,
Journal Year:
2022,
Volume and Issue:
151, P. 113119 - 113119
Published: May 24, 2022
Heavy
metal
Chromium
(Cr),
can
adversely
affect
humans
and
their
health
if
accumulated
in
organs
of
the
body,
such
as
lungs,
liver,
kidneys.
Cr
(VI)
is
highly
toxic
has
a
higher
solubility
water
than
(III).
One
most
common
routes
for
exposure
through
inhalation
associated
with
lung,
kidney
damage,
widespread
dermatitis,
GI
tract
human
lung
cancer,
cardiomyopathies,
cardiovascular
disease.
The
increase
ROS
production
been
attributed
to
damage
caused
by
toxicity.
Cr-induced
ROS-mediated
oxidative
stress
seen
cause
redox
imbalance
affecting
antioxidant
system
balance
body.
Nrf2
pathway
dysregulation
implicated
same.
Deregulation
histone
acetylation
methylation
observed,
together
gene
genes
p16,
MGMT,
APC,
hMLH1,
also
miR-143
repression.
Several
ultra-structural
changes
have
observed
following
(VI)-toxicity,
including
rough
ER
dilation,
alteration
mitochondrial
membrane
nuclear
membrane,
pycnotic
nuclei
formation,
cytoplasm
vacuolization.
A
significant
change
was
metabolism
lipid,
glucose,
protein
after
Cr.
Cr-toxicity
leads
immune
dysregulations
expression
IL-8,
IL-4,
IgM,
lymphocytes,
leukocytes
among
others.
P53,
well
pro-and
anti-apoptotic
proteins,
are
involved
apoptosis.
These
damages
be
alleviated
via
agents
that
restore
balance,
regulate
Nrf-2
levels,
or
proteins
while
decreasing
pro-apoptotic
proteins.
Ecotoxicology and Environmental Safety,
Journal Year:
2020,
Volume and Issue:
207, P. 111501 - 111501
Published: Nov. 3, 2020
Deltamethrin
(DLM)
is
widely
used
in
agriculture
and
the
prevention
of
human
insect-borne
diseases.
However,
molecular
mechanism
DLM
induced
liver
injury
remains
unclear
to
date.
This
study
investigated
potential
that
fibrosis
quails.
Japanese
quails
received
resveratrol
(500
mg/kg)
daily
with
or
without
(45
exposure
for
12
weeks.
Histopathology,
transmission
electron
microscopy,
biochemical
indexes,
TUNEL,
quantitative
real-time
PCR,
western
blot
analysis
were
performed.
hepatic
steatosis,
oxidative
stress,
inflammation,
apoptosis.
Most
importantly,
Nrf2/TGF-β1/Smad3
signaling
pathway
played
an
important
role
on
DLM-induced
Interestingly,
addition
resveratrol,
Nrf2
activator,
alleviates
stress
inflammation
response
by
activating
Nrf2,
thereby
inhibits
Collectively,
these
findings
demonstrate
chronic
induces
via
expression
inhibition
apoptosis,
then
results
activation
NF-κB/TNF-α
TGF-β1/Smad3
pathway.
Journal of Advanced Research,
Journal Year:
2021,
Volume and Issue:
35, P. 129 - 140
Published: April 8, 2021
Hexavalent
chromium
(Cr(VI)),
one
of
the
toxic
heavy
metals,
poses
a
serious
threat
to
human
and
animal
health.
Protein
acetylation
regulates
structure
function
most
proteins
in
variety
ways.
However,
hepatotoxicity
Cr(VI)
whether
it
is
related
deacetylation
remains
largely
unknown.We
aimed
explore
link
between
silent
information
regulator
two
ortholog
1
(Sirt1)
induced
by
exposure,
better
clarify
biological
mechanism
liver
injury
Cr(VI).We
established
model
K2Cr2O7
injecting
rats
intraperitoneally
for
35
days
continuously
adding
resveratrol
(Res)
further
hepatotoxicity.The
results
revealed
that
inflammatory
response
apoptosis
hepatocytes.
Furthermore,
reduced
Sirt1
expression
inhibited
downstream
key
transcription
factors,
including
nuclear
factor
erythroid
2-related
2
(Nrf2),
Forkhead
box
O3
(FOXO3),
factor-kappa
B
(NF-κB).
Conversely,
when
Res
was
administered
as
an
activator
Sirt1,
enhanced,
were
significantly
alleviated.In
summary,
this
work
firstly
demonstrates
induces
rat
inhibiting
which
positive
significance
protecting
natural
environment
health
from
chronic
Cr
poisoning.
