Immunoregulatory role of platelet derivatives in the macrophage-mediated immune response DOI Creative Commons
Eduardo Anitua, María Troya, Mohammad Hamdan Alkhraisat

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 25, 2024

Background Macrophages are innate immune cells that display remarkable phenotypic heterogeneity and functional plasticity. Due to their involvement in the pathogenesis of several human conditions, macrophages considered be an attractive therapeutic target. In line with this, platelet derivatives have been successfully applied many medical fields as active participants immunity, cooperation between platelets is essential. this context, aim review compile current evidence regarding effects on phenotype functions identify advantages shortcomings for feasible future clinical applications. Methods A total 669 articles were identified during systematic literature search performed PubMed Web Science databases. Results 27 met inclusion criteria. Based published findings, may play important role inducing a dynamic M1/M2 balance promoting timely M1-M2 shift. However, differences procedures polarization occasional lack information, makes reproducibility comparison results extremely challenging. Furthermore, understanding those derived from animal models, taking into account peculiarities tissue resident ontogeny seem essential design new strategies. Conclusion Research combination provides relevant information function mechanisms response.

Language: Английский

Engineered Exosome-Functionalized Extracellular Matrix-Mimicking Hydrogel for Promoting Bone Repair in Glucocorticoid-Induced Osteonecrosis of the Femoral Head DOI
Changjun Chen,

Li Fu,

Yue Luo

et al.

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(24), P. 28891 - 28906

Published: June 12, 2023

Glucocorticoid-induced osteonecrosis of the femoral head (GC-ONFH) is a serious bone disease that often affects young individuals. Bone grafting combined with core decompression mainly used in clinic to treat GC-ONFH. However, outcome usually not satisfactory, as expected. Here, we report an engineered exosome-functionalized extracellular matrix-mimicking hydrogel for promoting repair Compared Con-Exo, exosomes secreted by marrow stem cells (BMSCs) conventional culture medium, Li-Exo, derived from stimulated lithium ions, promoted macrophage M2 polarization while inhibiting M1 polarization. Furthermore, inspired fact hydrogels can serve desirable carriers facilitate their release sustained manner improved therapeutic efficiency and vivo application, matrix (ECM)-mimicking (Lightgel) composed methacryloylated type I collagen was employed incorporate Li-Exo/Con-Exo construct Lightgel-Li-Exo hydrogel/Lightgel-Con-Exo hydrogel. In vitro studies showed had most significant pro-osteogenic pro-angiogenic activity. Finally, evaluated effects rat models As result, effect on enhancing polarization, osteogenesis, angiogenesis promote Taken together, this novel ECM-mimicking could be promising strategy treatment.

Language: Английский

Citations

40
Advances in Platelet-Rich Plasma Treatment for Spinal Diseases: A Systematic Review DOI Open Access
Soya Kawabata, Koji Akeda, J. Yamada

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7677 - 7677

Published: April 21, 2023

Spinal diseases are commonly associated with pain and neurological symptoms, which negatively impact patients’ quality of life. Platelet-rich plasma (PRP) is an autologous source multiple growth factors cytokines, the potential to promote tissue regeneration. Recently, PRP has been widely used for treatment musculoskeletal diseases, including spinal in clinics. Given increasing popularity therapy, this article examines current literature basic research emerging clinical applications therapy treating diseases. First, we review vitro vivo studies, evaluating repairing intervertebral disc degeneration, promoting bone union fusion surgeries, aiding recovery from cord injury. Second, address degenerative disease, its analgesic effect on low back radicular pain, as well accelerating during surgery. Basic demonstrates promising regenerative PRP, studies have reported safety efficacy several Nevertheless, further high-quality randomized controlled trials would be required establish evidence therapy.

Language: Английский

Citations

31
An Engineered Bionic Nanoparticle Sponge as a Cytokine Trap and Reactive Oxygen Species Scavenger to Relieve Disc Degeneration and Discogenic Pain DOI Creative Commons
Wenbo Yang, Kanglu Li, Qing Pan

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(4), P. 3053 - 3072

Published: Jan. 18, 2024

The progressive worsening of disc degeneration and related nonspecific back pain are prominent clinical issues that cause a tremendous economic burden. Activation reactive oxygen species (ROS) inflammation is primary pathophysiologic change in degenerative lesions. This pathological state associated with M1 macrophages, apoptosis nucleus pulposus cells (NPC), the ingrowth pain-related sensory nerves. To address discogenic pain, we developed MnO2@TMNP, nanomaterial encapsulated MnO2 nanoparticles TrkA-overexpressed macrophage cell membrane (TMNP). Consequently, this engineered showed high efficiency binding various inflammatory factors nerve growth factors, which inhibited inflammation-induced NPC apoptosis, matrix degradation, ingrowth. Furthermore, provided specific targeting to macrophages for delivery nanoparticles. effectively scavenged intracellular ROS prevented polarization. Supportively, found MnO2@TMNP promoted regeneration, leading downregulated grades rat injured model. Both mechanical thermal hyperalgesia were alleviated by was attributed reduced calcitonin gene-related peptide (CGRP) substance P expression dorsal root ganglion Glial Fibrillary Acidic Protein (GFAP) Fos Proto-Oncogene (c-FOS) signaling spinal cord. We confirmed immune microenvironment intervertebral discs progression degeneration, resulting relieved pain.

Language: Английский

Citations

11
A new strategy for intervertebral disc regeneration: The synergistic potential of mesenchymal stem cells and their extracellular vesicles with hydrogel scaffolds DOI Open Access
Yan Zhao,

Huaize Dong,

Qiuqiu Xia

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 172, P. 116238 - 116238

Published: Feb. 3, 2024

Intervertebral disc degeneration (IDD) is a disease that severely affects spinal health and prevalent worldwide. Mesenchymal stem cells (MSCs) their derived extracellular vesicles (EVs) have regenerative potential emerged as promising therapeutic tools for treating degenerative discs. However, challenges such the harsh microenvironment of degenerated intervertebral discs EVs' limited stability efficacy hindered clinical application. In recent years, hydrogels attracted much attention in field IDD therapy because they can mimic physiologic provide solution by providing suitable growth environment MSCs EVs. This review introduced biological properties EVs, summarized research on application EVs IDD, current trial studies also explored mechanism action addition, plenty elaborated different classified tissue engineering, synergistic effect promoting regeneration, wide IDD. Finally, problems still faced hydrogel-loaded treatment are summarized, solutions proposed. paper outlines effects combination with aims to theoretical references future related studies.

Language: Английский

Citations

11
Platelet-rich plasma-derived extracellular vesicles inhibit NF-κB/NLRP3 pathway-mediated pyroptosis in intervertebral disc degeneration via the MALAT1/microRNA-217/SIRT1 axis DOI

Xueqiang Tao,

Fen Xue,

Jiayuan Xu

et al.

Cellular Signalling, Journal Year: 2024, Volume and Issue: 117, P. 111106 - 111106

Published: Feb. 17, 2024

Language: Английский

Citations

10
Dissolvable microneedle-based wound dressing transdermally and continuously delivers anti-inflammatory and pro-angiogenic exosomes for diabetic wound treatment DOI Creative Commons
Yanpeng Cao, Bei Chen,

Qixing Liu

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 42, P. 32 - 51

Published: Aug. 27, 2024

Due to overactive inflammation and hindered angiogenesis, self-healing of diabetic wounds (DW) remains challenging in the clinic. Platelet-derived exosomes (PLT-Exos), a novel exosome capable anti-inflammation pro-angiogenesis, show great potential DW treatment. However, previous administration into skin is topical daub or intradermal injection, which cannot intradermally deliver PLT-Exos dermis layer, thus impeding its long-term efficacy pro-angiogenesis. Herein, dissolvable microneedle-based wound dressing (PLT-Exos@ADMMA-MN) was developed for transdermal delivery PLT-Exos. Firstly, photo-crosslinking methacrylated acellular dermal matrix-based hydrogel (ADMMA-GEL), showing physiochemical tailorability, fast-gelling performance, excellent biocompatibility, pro-angiogenic capacities, synthesized as base material our dressing. For endowing with were encapsulated ADMMA-GEL minimum effective concentration determined by in-vitro experiments. Then, results that this exhibits properties Lastly, in-vivo experiments showed could continuously transdermally switch local macrophage M2 phenotype while stimulating neovascularization, proving low-inflammatory microenvironment healing. Collectively, study provides suppressing vascularization

Language: Английский

Citations

9
Role of macrophage in intervertebral disc degeneration DOI Creative Commons

Yiming Dou,

Yiming Zhang, Yang Liu

et al.

Bone Research, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 23, 2025

Language: Английский

Citations

1
MALAT1 participates in the role of platelet-rich plasma exosomes in promoting wound healing of diabetic foot ulcer DOI
Changhong Chen, Qinghua Wang,

Daibin Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 238, P. 124170 - 124170

Published: March 22, 2023

Language: Английский

Citations

20
Macrophages and platelets in liver fibrosis and hepatocellular carcinoma DOI Creative Commons
Martina Casari,

Dominik Siegl,

Carsten Deppermann

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 5, 2023

During fibrosis, (myo)fibroblasts deposit large amounts of extracellular matrix proteins, thereby replacing healthy functional tissue. In liver this leads to the loss hepatocyte function, portal hypertension, variceal bleeding, and increased susceptibility infection. At an early stage, fibrosis is a dynamic reversible process, however, from cirrhotic there significant progression hepatocellular carcinoma. Both liver-resident macrophages (Kupffer cells) monocyte-derived are important drivers progression, but can also induce its regression once triggers chronic inflammation eliminated. cancer, they attracted tumor site become tumor-associated (TAMs) polarized towards M2- anti-inflammatory/tumor-promoting phenotype. Besides their role in thrombosis hemostasis, platelets stimulate development by secreting profibrogenic factors regulating innate immune response, e.g., interacting with monocytes macrophages. Here, we review recent literature on interplay

Language: Английский

Citations

19
Platelet, a key regulator of innate and adaptive immunity DOI Creative Commons

Cheng Yan,

Haojie Wu,

Xianchun Fang

et al.

Frontiers in Medicine, Journal Year: 2023, Volume and Issue: 10

Published: March 10, 2023

Platelets, anucleate blood components, represent the major cell type involved in regulation of hemostasis and thrombosis. In addition to performing haemostatic roles, platelets can influence both innate adaptive immune responses. this review, we summarize development their functions hemostasis. We also discuss interactions between platelet products or cells, including neutrophils, monocytes, macrophages, T B cells dendritic cells. Activated released molecules regulate differentiation function these via platelet-derived receptors secreting molecules. Platelets have dual effects on nearly all Understanding exact mechanisms underlying will enable further application transfusion.

Language: Английский

Citations

18