Succinate exacerbates mastitis in mice via gut microbiota-derived extracellular vesicles DOI Creative Commons

Min Qiu,

Yubo Hu, Chen‐Yu Zou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 16, 2023

Abstract Background Ecological imbalance of the gut microbiota may be an important endogenous trigger mastitis, but underlying mechanisms are unclear. Recent findings indicated that selected bacteria maternal gastrointestinal can reach mammary gland through entero-mammary pathway. In addition, metabolic disturbances one most prominent ways in which dysbiosis promote distal organ diseases. Results present study, we found Gastrointestinal-associated mastitis showed significant changes levels, among succinate was significantly increased. treatment exacerbates endotoxemia-induced mice. Meanwhile, induced intestinal ecological dysregulation. Further experiments demonstrated increased production extracellular vesicles containing microbial (mEVs) lipopolysaccharides, could diffuse across damaged barrier into glands. mEVs promotes inflammation mainly activation TLR4/NF-κB Conclusions Our suggest proliferatio enteric pathogens and production, suggesting a potential strategy for intervention based on regulation pathogen inhibition. The role interspecific communication has also been elucidated.

Language: Английский

Unveiling distinct genetic features in multidrug-resistant Escherichia coli isolated from mammary tissue and gut of mastitis induced mice DOI Creative Commons
M. Nazmul Hoque, Golam Mahbub Faisal,

Shobnom Jerin

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e26723 - e26723

Published: Feb. 21, 2024

is one of the major pathogens causing mastitis in lactating mammals. We hypothesized that

Language: Английский

Citations

11
Diosmetin alleviates S. aureus-induced mastitis by inhibiting SIRT1/GPX4 mediated ferroptosis DOI
Lihua Zhao,

Lei Jin,

Bin Yang

et al.

Life Sciences, Journal Year: 2023, Volume and Issue: 331, P. 122060 - 122060

Published: Aug. 29, 2023

Language: Английский

Citations

22
Effect and mechanism of Prunella vulgaris L. extract on alleviating lipopolysaccharide-induced acute mastitis in protecting the blood-milk barrier and reducing inflammation DOI

Hongshan Luo,

Yamei Li, Jingchen Xie

et al.

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 328, P. 117998 - 117998

Published: March 12, 2024

Language: Английский

Citations

5
Diet influences knee osteoarthritis osteophyte formation via gut microbiota and serum metabolites DOI Creative Commons

Dandan Zhu,

Xiaochao Wang,

Zhihui Xi

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 110111 - 110111

Published: May 24, 2024

Osteophyte formation, a key indicator of osteoarthritis (OA) severity, remains poorly understood in its relation to gut microbiota and metabolites knee (KOA). We conducted 16S rDNA sequencing untargeted metabolomics on fecal serum samples from 20 healthy volunteers, 80 KOA patients Guangdong, 100 Inner Mongolia, respectively. Through bioinformatics analysis, we identified 3 genera 5 associated with osteophyte formation. Blautia abundance negatively correlated meat, cheese, bean consumption. The dairy, beef, sugar, salt intake, yet positively age oil Higher levels the may contribute LTB4 PGD2 potentially serving as biomarkers. Mongolia exhibited lower reduced expression metabolites, possibly due cheese consumption habits, resulting less

Language: Английский

Citations

5
Phlorizin Limits Bovine Viral Diarrhea Virus Infection in Mice via Regulating Gut Microbiota Composition DOI
Zhicheng Zhao, Chuang Li,

Jiang Huang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 9906 - 9914

Published: April 16, 2024

Phlorizin (PHZ) is one of the main pharmacologically active ingredients in

Language: Английский

Citations

4
Integrated microbiome and metabolome analysis reveals altered gut microbial communities and metabolite profiles in dairy cows with subclinical mastitis DOI Creative Commons
Jie Yu, Chenhui Liu, Dingfa Wang

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 4, 2025

Dairy cow mastitis is a common and prevalent disease arose by various complicated pathogeny, which poses serious threat to the health of cows, safety dairy product economic benefits for pastures. Due high stealthiness long incubation period, subclinical (SM) cows causes enormous losses. Besides infection exogenous pathogenic microorganisms, previous studies demonstrated that gastrointestinal microbial dysbiosis one crucial occurrence development based on theory entero-mammary axis. Whereas, limited researches have been conducted potential pathological metabolic mechanisms underlying relationship between gut microbiota SM in cows. The differences blood parameters, microbiome, plasma fecal metabolome healthy were compared performing 16 S rDNA sequencing non-targeted metabolomic analysis current study. content total cholesterol (TC), high-density lipoprotein (HDL-C), activity catalase (CAT), antioxidant capacity(T-AOC) significantly decreased, while malondialdehyde (MDA) concentration was dramatically increased serum comparison with harbored more abundant Cyanobacteria, Proteobacteria, Succinivibrio Lactobacillus_iners. Moreover, abundance Paraprevotella, Coprococcus, Succiniclasticum, Desulfovibrio Bifidobacterium_pseudolongum observably reduced Furthermore, higher pro-inflammatory metabolites observed feces (9(S)-HPODE, 25-hydroxycholesterol, dodecanedioic acid, etc.) (9-hydroxy-10,12-octadecadienoic 13,14-dihydro PGF1α, 5,6-dehydro arachidonic myristic histamine, certain anti-inflammatory properties (mandelic gamma-tocotrienol, deoxycholic notably decreased or SM. intestinal composition profiles distinct, characterized symbiotic bacteria, probiotics anti-inflammatory, compounds, along lipid metabolites, oxidative stress suffered from results this study further enriched our understanding correlations SM, provided insight into formulation management strategies

Language: Английский

Citations

0
Rumen microbes affect the somatic cell counts of dairy cows by modulating glutathione metabolism DOI Creative Commons
Hua Zhang,

Tianhang Lu,

Shijiao Guo

et al.

mSystems, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

ABSTRACT Healthy mammary glands are essential for high-quality milk production in the dairy industry. The relationship between somatic cell counts (SCCs), rumen fermentation, and microbiota interactions remains unclear. This study integrated physiological indicators, high-throughput 16S rRNA gene sequencing, metagenomics data analysis to investigate mechanisms linking microbes mastitis evaluate changes serum cytokine levels cows with low (L-SCC) high (H-SCC) counts. Compared L-SCC group, H-SCC group exhibited significantly lower lactose fat contents milk, reduced fermentation product levels, increased abundances of Bacteroidetes, Firmicutes, Lachnospiraceae , Prevotella Rumiclostridium. Elevated IgG2, IgM, IL-1β, IL-6, TNF-ɑ indicated inflammation dysbiosis. Functional microbial communities revealed significant enrichment pathways related glutathione metabolism, thyroid hormone synthesis, hypertrophic cardiomyopathy (HCM), phosphotransferase system (PTS), P53 signaling pathway, Jak-STAT pathway. Correlation network showed that bacterial families, such as Rikenellaceae, Muribaculaceae, Prevotellaceae were associated cytokines, quality. highlights interaction homeostasis gland health, indicating status influences Modulating enhance immune function presents a viable strategy sustainable industry development long-lived, highly productive cows. IMPORTANCE High (SCCs) key biomarker decreased economic losses farming. systematically examines elevated SCCs, dysbiosis, host inflammatory responses, shedding light on intricate interplay ecosystems physiology. findings highlight potential microbiota-targeted interventions reduce inflammation, improve composition, cow productivity. Rather than presuming direct causative link SCC-associated dysbiosis this research focuses their interdependent dynamics, offering nuanced understanding complex biological involved. work advances knowledge host-microbiota livestock, providing practical insights innovative strategies manage overall herd health. By adhering One Health principles, underscores significance agricultural practices prioritize animal welfare, environmental stewardship, food security. These establish robust foundation future into microbiota-driven solutions aimed at enhancing health productivity cattle.

Language: Английский

Citations

0
Dietary Angelica sinensis Enhances Sow Lactation and Piglet Development Through Gut Microbiota and Metabolism DOI Creative Commons
Qian Chen,

Yali Song,

Qinghua Wu

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 370 - 370

Published: April 15, 2025

Piglets weaned per sow year (PSY) is a crucial metric for assessing the reproductive performances of sows and directly affects both productivity economic profitability pig farms [...]

Language: Английский

Citations

0
Angelica sinensis Polysaccharide Alleviates Staphylococcus aureus-Induced Mastitis by Regulating The Intestinal Flora and Gut Metabolites DOI
Xin Ran, Yutao Li, Weiwei Guo

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 25, 2024

The modulation of intestinal flora by various polysaccharides has been shown to mitigate disease progression. Recent research reveals a significant link between and the progression mastitis. This study demonstrates that oral administration

Language: Английский

Citations

3
The rumen microbiota contributed to the development of mastitis induced by subclinical ketosis DOI
Ruibo Tang, Wencheng Yang, Jianhua Song

et al.

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 187, P. 106509 - 106509

Published: Jan. 5, 2024

Language: Английский

Citations

2