Advances in cancer research, Journal Year: 2024, Volume and Issue: unknown, P. 137 - 185
Published: Jan. 1, 2024
Language: Английский
Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)
Published: June 14, 2024
Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.
Language: Английский
Citations
35
Cell Communication and Signaling, Journal Year: 2025, Volume and Issue: 23(1)
Published: Feb. 11, 2025
Extracellular vesicles (EVs) are relevant elements for cell-to-cell communication and considered crucial in host-pathogen interactions by transferring molecules between the pathogen host during infections. These structures participate various physiological pathological processes promising candidates as disease markers, therapeutic reagents, drug carriers. Both H. pylori epithelial cells infected secrete EVs, which contribute to inflammation development of phenotypes. However, many aspects cellular molecular biology EV functions remain incompletely understood due methodological challenges studying these small structures. This review also highlights roles EVs derived from pylori-infected pathogenesis gastric extragastric diseases. Understanding specific infections, whether advantageous or pathogen, may help new approaches prevent disease.
Language: Английский
Citations
2
International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 3657 - 3675
Published: April 1, 2024
Abstract: Exosomes are extracellular vesicles that originate from various cells and mediate intercellular communication, altering the behavior or fate of recipient cells. They carry diverse macromolecules, such as lipids, proteins, carbohydrates, nucleic acids. Environmental stressors can change exosomal contents many cells, making them useful for diagnosing chronic disorders, especially neurodegenerative, cardiovascular, cancerous, diabetic diseases. Moreover, exosomes be engineered therapeutic agents to modulate disease processes. State-of-art techniques employed separate including ultracentrifugation, size-exclusion chromatography immunoaffinity. However, modern technologies aqueous two-phase system well microfluidics gaining attention in recent years. The article highlighted composition, biogenesis, implications exosomes, standard novel methods isolating applying biomarkers cargo carriers. Keywords: Exosomes, vesicles, biomarkers, carriers, separation
Language: Английский
Citations
12
RSC Advances, Journal Year: 2024, Volume and Issue: 14(42), P. 30807 - 30829
Published: Jan. 1, 2024
Modified exosomes are a smart tool for the upcoming precision cancer therapeutic era.
Language: Английский
Citations
9
Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 7, 2025
Language: Английский
Citations
1
The Journal of Liquid Biopsy, Journal Year: 2025, Volume and Issue: unknown, P. 100293 - 100293
Published: March 1, 2025
Liquid biopsy (LB) has emerged as a transformative tool in oncology, providing minimally invasive approach for tumor detection, molecular characterization, and real-time treatment monitoring. By analyzing circulating DNA (ctDNA), cells (CTCs), extracellular vesicles (EVs), microRNA (miRNA), LB enables comprehensive profiling without the need traditional tissue biopsies. Over past decade, research this field expanded exponentially, leading to integration of into clinical practice specific cancer types, including lung breast cancer. In 2024, Journal Biopsy (JLB) published innovative studies exploring latest advancements technologies, biomarkers, their applications minimal residual disease (MRD) monitoring, therapy response assessment. This review synthesizes recent findings on role monitoring across different with particular focus newly context within translational research. Additionally, it highlights emerging techniques such fragmentomics, artificial intelligence, multiomics, paving way more precise, personalized decisions. Despite these advancements, challenges remain standardizing methodologies, optimizing validation, integrating routine oncological workflows. mini-review evolving landscape its potential revolutionize diagnosis, therapeutic decision-making, ushering new era precision oncology.
Language: Английский
Citations
1
ACS Applied Bio Materials, Journal Year: 2024, Volume and Issue: 7(3), P. 1400 - 1415
Published: Feb. 23, 2024
Exosomes are a subpopulation of extracellular vesicles (EVs) secreted by cells. In cancer, they key cellular messengers during cancer development and progression. Tumor-derived exosomes (TEXs) promote oral the major complication is squamous cell carcinoma (OSCC). show strong participation in several OSCC-related activities such as uncontrolled growth, immune suppression, angiogenesis, metastasis, drug therapeutic resistance. It also potential biomarker source for cancer. Some exosome sources stem cells, plants (it more effective compared to others), engineered reduce development. This approach because its specificity, biocompatibility, cell-free therapy reduced side effects treatment). article highlights exosome-based theranostics signatures clinical trials, challenges research, future improvements. future, may become an affordable solution
Language: Английский
Citations
5
Cytopathology, Journal Year: 2024, Volume and Issue: 35(6), P. 664 - 670
Published: June 1, 2024
The transformative role of artificial intelligence (AI) and multiomics could enhance the diagnostic prognostic capabilities liquid biopsy (LB) for lung cancer (LC). Despite advances, transition from tissue biopsies to more sophisticated, non-invasive methods like LB has been impeded by challenges such as heterogeneity biomarkers low concentration tumour-related analytes. advent - enabled deep learning algorithms offers a solution allowing simultaneous analysis various analytes across multiple biological fluids, presenting paradigm shift in diagnostics. Through multi-marker, multi-analyte multi-source approaches, this review showcases how AI are identifying clinically valuable biomarker combinations that correlate with patients' health statuses. However, path towards clinical implementation is fraught challenges, including study reproducibility lack methodological standardization, thus necessitating urgent solutions solve these common issues.
Language: Английский
Citations
4
FEBS Letters, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 17, 2025
Extracellular vesicles (EVs) are released by all kind of cells into the extracellular space, where they shuttle parental cell‐derived molecular cargoes (DNA, RNA, proteins) to both adjacent and distant cells, influencing physiology target cells. Their specific cargo content abundance in liquid biopsies make them excellent candidates for biomarker studies. Indeed, EVs isolated from various body fluids, including blood, pleural fluid, urine, cerebrospinal saliva, milk, ascites, tears, have been recognized their potential as biomarkers diagnosis, monitoring treatment, predicting outcomes diseases. Increasing studies suggest that tears great promise a noninvasive biopsy source EVs. Our aim here is provide comprehensive review exploration reservoir contents, evaluating accessibility utility method. Additionally, tear cancers, ocular cancer, discussed. Finally, advantages challenges employing tear‐based disease ' s evaluated.
Language: Английский
Citations
0
Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown
Published: March 17, 2025
Abstract Neurodegenerative diseases (NDs) like Alzheimer’s, Parkinson’s, and ALS rank among the most challenging global health issues, marked by substantial obstacles in early diagnosis effective treatment. Current diagnostic techniques frequently demonstrate inadequate sensitivity specificity, whilst conventional treatment strategies encounter challenges related to restricted bioavailability insufficient blood–brain barrier (BBB) permeability. Recently, exosomes—nanoscale vesicles packed with proteins, RNAs, lipids—have emerged as promising agents potential reshape therapeutic approaches these diseases. Unlike drug carriers, they naturally traverse BBB can deliver bioactive molecules affected neural cells. Their molecular cargo influence cell signaling, reduce neuroinflammation, potentially slow neurodegenerative progression. Moreover, exosomes serve non-invasive biomarkers, enabling precise while allowing real-time disease monitoring. Additionally, engineered exosomes, loaded molecules, enhance this capability targeting diseased neurons overcoming barriers. By offering enhanced reduced immunogenicity, an ability bypass physiological limitations, exosome-based present a transformative advantage over existing approaches. This review examines multifaceted role of NDDs, emphasizing their capabilities, intrinsic functions, advanced vehicles.
Language: Английский
Citations
0