miR-214-3p Promotes ox-LDL-Induced Macrophages Ferroptosis and Inflammation via GPX4 DOI Creative Commons
Xueliang Pei, Facai Cui, Yu‐Kuo Chen

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 3937 - 3950

Published: March 1, 2025

Atherosclerosis (AS) is a chronic inflammatory disease caused by the dysregulation of lipid metabolism. It has been established that oxidized low-density lipoprotein (ox-LDL)-induced macrophage inflammation and ferroptosis play important roles in AS. However, mechanism which ox-LDL induces macrophages requires further investigation. A foam cell model derived from ox-LDL-induced was constructed to study its action. The levels interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α were evaluated using an Enzyme-Linked Immunosorbent Assay (ELISA). Oil Red O staining used detect intracellular accumulation levels. Lactate dehydrogenase (LDH), malondialdehyde (MDA), reactive oxygen species (ROS), Fe2+ assessed. Dual-luciferase RNA-binding protein immunoprecipitation (RIP) experiments validated binding relationship between microRNA (miR)-214-3p glutathione peroxidase 4 (GPX4). IL-6, TNF-α significantly increased macrophages, accompanied accumulation, indicating promotion formation. Additionally, LDH, MDA, ROS, Fe2+. expression miR-214-3p positively correlated with concentration macrophages. Treatment inhibitor reduces responses, RIP confirmed regulates GPX4 transcription. Silenced reversed effects on Low also promotes induced ox-LDL. This may be associated miR-214-3p-induced expression, underscores therapeutic significance targeting addressing

Language: Английский

Molecular mechanisms of ferroptosis and relevance to inflammation DOI Open Access

Liyan Deng,

Shasha He,

Nuoqing Guo

et al.

Inflammation Research, Journal Year: 2022, Volume and Issue: 72(2), P. 281 - 299

Published: Dec. 19, 2022

Language: Английский

Citations

139
The emerging role of ferroptosis in female reproductive disorders DOI Creative Commons
Min Liu, WU Ke-ming,

Yeke Wu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2023, Volume and Issue: 166, P. 115415 - 115415

Published: Sept. 4, 2023

Iron, as an essential trace element for the organism, is vital maintaining organism's health. Excessive iron can promote reactive oxygen species (ROS) accumulation, thus damaging cells and tissues. Ferroptosis a novel form of programmed cell death distinguished by overload lipid peroxidation, which unique from autophagy, apoptosis necrosis, more studies are focusing on ferroptosis. Recent evidence suggests that ferroptosis associated with development female reproductive disorders (FRDs), including polycystic ovary syndrome (PCOS), premature ovarian insufficiency (POI), endometriosis (EMs), cancer (OC), preeclampsia (PE) spontaneous abortion (SA). Pathways genes may participate in processes regulate granulosa proliferation secretion, oocyte development, reserve function, early embryonic placental oxidative stress. However, its exact mechanism has not been fully revealed. Therefore, our review systematically elaborates occurrence research progress FRDs, view to providing literature references clinical targeting -related pathways regulatory factors management FRDs.

Language: Английский

Citations

42
The protective effect of Enteromorpha prolifera polysaccharide on alcoholic liver injury in C57BL/6 mice DOI
Tingting Yan, Yuying Zhang, Hengyu Lu

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 261, P. 129908 - 129908

Published: Feb. 4, 2024

Language: Английский

Citations

22
Astaxanthin activates the Nrf2/Keap1/HO-1 pathway to inhibit oxidative stress and ferroptosis, reducing triphenyl phosphate (TPhP)-induced neurodevelopmental toxicity DOI Creative Commons
Qiong Zhang,

Congying Luo,

Zhikang Li

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 271, P. 115960 - 115960

Published: Jan. 16, 2024

Triphenyl phosphate (TPhP) serves as a major organophosphorus flame retardant, and its induced neurodevelopmental toxicity has attracted widespread attention, but the mechanism remains unclear. In this study, we involved zebrafish to explore new of TPhP inducing oxidative stress ferroptosis promote toxicity. The results suggested that affected embryonic development, reduced number neurons, led abnormal neural behavior in larvae. also ROS accumulation, activated antioxidant defense signal Nrf2 Keap1, significantly changed activities Acetylcholinesterase (AChE), Adenosine triphosphatase (ATPase) glutathione S-transferase (GST). addition, zebrafish, which was reflected increase Fe2+ content, expression GPX4 protein genes related iron metabolism (gpx4a, slc7a11, acsl4b, tfa, slc40a1, fth1b, tfr2, tfr1a, tfr1b ncoa4). Astaxanthin intervention specifically inhibited levels, reversed SLC7A11 levels thus alleviating by TPhP. partially activity AChE, GST neurodevelopmental-related (gap43, gfap, neurog1 syn2a), so rescue developmental abnormalities motor disorders More interestingly, mitochondrial apoptosis-related BAX, anti-apoptotic BCL-2, Caspase3 Caspase9 altered exposed group, could be intervention. summary, our exposure can induce ferroptosis, thereby causing neurodevelopment while reverse reduce larvae activating Nrf2/Keap1/HO-1 signaling pathway.

Language: Английский

Citations

18
Hyperoxia-activated Nrf2 regulates ferroptosis in intestinal epithelial cells and intervenes in inflammatory reaction through COX-2/PGE2/EP2 pathway DOI Creative Commons
Yanping Liu,

Tianming Li,

Changping Niu

et al.

Molecular Medicine, Journal Year: 2025, Volume and Issue: 31(1)

Published: Jan. 3, 2025

Language: Английский

Citations

2
Stigmasterol from Prunella vulgaris L. Alleviates LPS-Induced Mammary Gland Injury by Inhibiting Inflammation and Ferroptosis DOI
Mingyang Sun, Dianwen Xu, Dianfeng Liu

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 137, P. 156362 - 156362

Published: Jan. 5, 2025

Language: Английский

Citations

2
Tangeretin alleviates sepsis-induced acute lung injury by inhibiting ferroptosis of macrophage via Nrf2 signaling pathway DOI Creative Commons
Hui Zhang, Yan Wang, Shenghua Wang

et al.

Chinese Medicine, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 15, 2025

Abstract Background Sepsis-induced acute lung injury (ALI) is a severe clinical condition accompanied with high mortality. Tangeretin, which widely found in citrus fruits, has been reported to exert antioxidant and anti-inflammatory properties. However, whether tangeretin protects against sepsis-induced ALI the potential mechanisms remain unclear. Methods We established an model via intraperitoneally injected 5 mg/kg lipopolysaccharides (LPS) for 12 h. Tangeretin was applied 30 min before LPS treatment. Dexamethasone (Dex) used as positive control. Hematoxylin eosin (HE) staining protein content bronchoalveolar lavage fluid (BALF) were determined detect degree of injury. RNA-seq also explore effect on ALI. In vitro, RAW264.7 treated Nrf2 siRNA, expression ferroptosis-associated biomarkers, including glutathione peroxidase 4 (GPX4) prostaglandin-endoperoxide synthase 2 (PTGS2) assessed. Glutathione (GSH), malondialdehyde (MDA) levels, reactive oxygen species (ROS) inflammatory factors both vivo vitro. Furthermore, mice inhibitor (ML385) verify mechanism inhibiting ferroptosis. Data analyzed using one way analysis variance or two-tailed unpaired t tests. Results Our study demonstrated that significantly alleviated injury, reversed LPS-induced reduction GPX4 GSH, mitigates elevation PTGS2 MDA levels. reduced 4-HNE iron Besides, levels LPS-stimulated IL-6, IL-1β TNF-α decreased by tangeretin. bioinformatics inhibited response. Mechanistically, we identified GPX4-dependent lipid peroxidation through activation Nrf2. The silence abolished inhibitory oxidative stress, response ferroptosis cells. Additionally, all protective effects inhibitor-treated mice. Conclusion critical contributing promising therapeutic candidate, effectively upregulating signaling pathway.

Language: Английский

Citations

2
Butyrate ameliorated ferroptosis in ulcerative colitis through modulating Nrf2/GPX4 signal pathway and improving intestinal barrier DOI Creative Commons

Hangping Chen,

Yifan Qian,

Chensheng Jiang

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2023, Volume and Issue: 1870(2), P. 166984 - 166984

Published: Dec. 6, 2023

Oxidative stress and intestinal inflammation are main pathological features of ulcerative colitis (UC). Ferroptosis, characterized by iron accumulation lipid peroxidation, is closely related to the pathologic process UC. 16S rRNA sequencing for microbiota analysis gas chromatography-mass spectrometry (GC-MS) short-chain fatty acid (SCFA) contents clearly demonstrated lower amounts butyrate-producing bacteria butyrate in mice. However, precise mechanisms sodium (NaB) treating UC remain largely unclear. We found that ferroptosis occurred models, as evidenced inflammatory response, intracellular level, mitochondria ultrastructural observations associated protein expression. NaB inhibited colitis, significantly rescued weight loss colon shortening mice reduced lesions mitochondrial damage. Furthermore, improved barrier integrity markedly suppressed expression pro-ferroptosis proteins. Conversely, anti-ferroptosis markers including nuclear factor erythroid-related Factor 2 (Nrf2) glutathione peroxidase 4 (GPX4), was upregulated with treatment. Moreover, knockdown Nrf2 reversed anti-colitis effect NaB. Taken together, exhibited a protective ameliorating experimental through Nrf2/GPX4 signaling improving integrity, which provides novel mechanism prevention

Language: Английский

Citations

34
Activation of Nrf2 pathway as a protective mechanism against oxidative stress-induced diseases: Potential of astaxanthin DOI
Osman N. Kanwugu, Т. В. Глухарева

Archives of Biochemistry and Biophysics, Journal Year: 2023, Volume and Issue: 741, P. 109601 - 109601

Published: April 20, 2023

Language: Английский

Citations

26
The Keap1/Nrf2/ARE/HO-1 axis in epilepsy: Crosstalk between oxidative stress and neuroinflammation DOI
Mohammad Amin Manavi, Razieh Mohammad Jafari,

Hamed Shafaroodi

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 153, P. 114304 - 114304

Published: March 20, 2025

Language: Английский

Citations

1