The role of confocal laser endomicroscopy in pulmonary medicine

European Respiratory Review, Journal Year: 2023, Volume and Issue: 32(167), P. 220185 - 220185

Published: Jan. 25, 2023

Accurate diagnosis and subsequent therapeutic options in pulmonary diseases mainly rely on imaging methods histological assessment. However, examinations are hampered by the limited spatial resolution of images most procedures that related to assessment invasive with associated complications. As a result, high-resolution technology – confocal laser endomicroscopy (CLE), which is at forefront enables real-time microscopic visualisation morphologies architectures tissues or cells has been developed resolve clinical dilemma pertaining current techniques. The evidence shown CLE potential facilitate advanced diagnostic capabilities, monitor aid tailored treatment regime for patients diseases, as well expand horizon unravelling mechanism targets diseases. In future, if can be combined artificial intelligence, early, rapid accurate will achieved through identifying automatically. promising this technique may be, further investigations required before it enter routine practice.

Language: Английский

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Three-Carbon Linked Dihydroartemisinin-Isatin Hybrids: Design, Synthesis and Their Antiproliferative Anticancer Activity

Frontiers in Pharmacology, Journal Year: 2022, Volume and Issue: 13

Published: Jan. 26, 2022

Fifteen dihydroartemisinin-isatin hybrids ( 5a-e and 6a-j) linked with three-carbon were designed, synthesized. The antiproliferative activity against lung cancer cell lines including drug-sensitive A549, doxorubicin-resistant A549 (A549/DOX) cisplatin-resistant (A549/DDP) was tested. cytotocivity towards normal epithelial BEAS-2B line also investigated. From the structure-activity relationship (SAR), it found that hydrogen bond donors (especially hydroxime thiosemicarbazide) at C-3 position electron-withdrawing groups (fluoro chloro) C-5 of isatin moiety beneficial for activity. A significant part them (half maximal inhibitory concentration/IC 50 : 5.72–55.52 μ M) demonstrated considerable activity, superior to dihydroartemisinin (IC 69.42–88.03 artemisinin >100 M). In particular, two 6a, e 5.72–9.84 not inferior doxorubicin 4.06 cisplatin 9.38 cells more potent than 54.32 15.10 19.74 66.89 multidrug-resistant A549/DOX A549/DDP lines. addition, showed no toxicity cells, proving their excellent selectivity profile. Furthermore, hybrid 6a possessed good stability in mouse human microsomes, as well pharmacokinetic properties. Accordingly, could serve a promising anti-lung chemotherapeutic candidate further preclinical evaluations.

Language: Английский

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Application of bronchoscopy in the diagnosis and treatment of peripheral pulmonary lesions in China: a national cross-sectional study

Journal of Cancer, Journal Year: 2023, Volume and Issue: 14(8), P. 1398 - 1406

Published: Jan. 1, 2023

Background: Bronchoscopy has gradually become valuable armamentarium in evaluating and applying endoscopic therapy to peripheral pulmonary lesions (PPLs) around the world.We aimed make a comprehensive understanding of application bronchoscopy diagnosis treatment PPLs China.Methods: A cross-sectional survey was carried out China between January 2022 March 2022.The form an online questionnaire which filled with real-time data by respondents.Results: total 347 doctors from 284 tertiary hospitals (81.8%) 63 secondary general (18.2%) were included analysis.More than half surveyed (55.0%) had independently performed respiratory endoscopy for 5-15 years.Higher proportions fixed nursing team, anesthesiologists rapid on-site evaluation (ROSE) during bronchoscopic procedures those (P<0.001each).There 316 (91.7%) eligible performing biopsies less 30mm, while more 300 only 78 (24.7%) per year.Radial probe endobronchial ultrasound (r-EBUS) (50.3%) commonest type technique used guidance bronchoscope PPLs, followed navigational (30.3%) cone beam CT (CBCT) (17.0%).Although two thirds at least one devices, actual utilization these devices not high due capital costs absence training.To note, diagnostic allocated concentrated southeast region coastal cities.Furthermore, therapeutic interventions lung cancer and/or high-risk could be 124 (35.7%) involved hospitals.Conclusions: been most yields different regions varied greatly.To date, few can develop PPLs.

Language: Английский

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Transbronchial lung cryobiopsy for peripheral pulmonary lesions. A narrative review

Pulmonology, Journal Year: 2023, Volume and Issue: 30(5), P. 475 - 484

Published: Oct. 31, 2023

An increasing number of peripheral pulmonary lesions (PPLs) requiring tissue verification to establish a definite diagnosis for further individualized management are detected due the growing adoption lung cancer screening by chest computed tomography (CT), especially low-dose CT. However, morphological PPLs remains challenging. Transbronchial cryobiopsy (TBLC) that can retrieve larger specimens with more preserved cellular architecture and fewer crush artifacts in comparison conventional transbronchial forceps biopsy (TBFB), as an emerging technology diagnosing PPLs, has been demonstrated have potential resolve clinical dilemma pertaining currently available sampling devices (e.g., forceps, needle brush) become diagnostic cornerstone PPLs. Of note, introduction 1.1 mm cryoprobe will be compatible advanced bronchoscopic navigation techniques, such radial endobronchial ultrasound (r-EBUS), virtual (VBN) electromagnetic bronchoscopy (ENB), use TBLC is expected gain popularity While much exploration using technique it envisaged emergence additional studies data accrual hopefully add body evidence this field.

Language: Английский

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miR-199a: A Tumor Suppressor with Noncoding RNA Network and Therapeutic Candidate in Lung Cancer

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(15), P. 8518 - 8518

Published: July 31, 2022

Lung cancer is the leading cause of death worldwide. miR-199a, which has two mature molecules: miR-199a-3p and miR-199a-5p, plays an important biological role in genesis development tumors. We collected recent research results on lung miR-199a from Google Scholar PubMed databases. The functions are reviewed detail, its potential roles diagnosis treatment discussed. With as core point a divergence outward, interplay between other ncRNAs reviewed, regulatory network covering various cancers depicted, can help us to better understand mechanism occurrence provide means for developing novel therapeutic strategies. In addition, current methods reviewed. Finally, conclusion was drawn: inhibits cancer, especially by inhibiting proliferation, infiltration, migration cells, tumor angiogenesis, increasing apoptosis affecting drug resistance cells. This review aims new insights into therapy prevention.

Language: Английский

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The anti-lung cancer activity of propylene tethered dihydroartemisinin-isatin hybrids

Arabian Journal of Chemistry, Journal Year: 2022, Volume and Issue: 15(4), P. 103721 - 103721

Published: Jan. 25, 2022

Nineteen propylene tethered dihydroartemisinin-isatin hybrids 5a-g and 6a-l were designed, synthesized, screened for their in vitro antiproliferative activity against three lung cancer cell lines, inclusive of drug-sensitive (A549), doxorubicin-resistant A549 (A549/DOX) cisplatin-resistant (A549/DDP) lines. The cytotoxicity the synthesized towards normal epithelial line (BEAS-2B) was also assessed to evaluate selectivity. structure-activity relationship (SAR) elucidated that (1) alkyloxylimino fragment at C-3 position isatin moiety more favorable than carbonyl benzoxylimino, relative contricution order methoxylimino > ethoxylimino benzoxylimino; halogen atom C-5 or C-6 could enhance activity. Among them, hybrid 6f (IC50: 21.7–28.9 μM) showed promising tested not inferior cisplatin 19.7 66.9 doxorubicin 54.3 15.1 multidrug-resistant A549/DOX A549/DDP In addition, >100 non-cytotoxic (BEAS-2B), RI values 1.12 1.33. Further, possessed acceptable stability mouse human microsomes. Accordingly, 6a a anti-lung chemotherapeutic candidate merited further evaluations.

Language: Английский

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Robotic bronchoscopic needle‐based confocal laser endomicroscopy to diagnose peripheral lung nodules

Respirology, Journal Year: 2022, Volume and Issue: 28(5), P. 475 - 483

Published: Dec. 19, 2022

Robotic bronchoscopy has demonstrated high navigational success in small peripheral lung nodules but the diagnostic yield is discrepantly lower. Needle based confocal laser endomicroscopy (nCLE) enables real-time microscopic imaging at needle tip. We aim to assess feasibility, safety and repositioning on nCLE-guidance during robotic nodules.

Language: Английский

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Ablation for Single Pulmonary Nodules, Primary or Metastatic. Εndobronchial Ablation Systems or Percutaneous

Journal of Cancer, Journal Year: 2024, Volume and Issue: 15(4), P. 880 - 888

Published: Jan. 1, 2024

Single pulmonary nodules are a difficult to diagnose imagining artifact.Currently novel diagnostic tools such as Radial-EBUS with or not C-ARM flouroscopy, electromagnetic navigation systems, robotic bronchoscopy and cone beam-compuer tomography (CBCT) can assist in the optimal guidance of biopsy equipment.After diagnosis lung cancer metastatic disease nodule, then surgery ablation methods local treatment be applied.The percutaneous systems under computed radiofrequency, microwave, cryo thermosphere have been used for several years.In past 10 years extensive research has made endobronchial methods.We will present comment on two different up date data.

Language: Английский

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Bibliometric and visual analysis of photodynamic therapy for lung cancer from 2010 to 2022

Translational Cancer Research, Journal Year: 2024, Volume and Issue: 13(2), P. 738 - 751

Published: Feb. 1, 2024

Background: A growing body of research shows that photodynamic therapy (PDT) has become an important mode treatment in the field lung cancer (LC) recent years. Although numerous papers related to PDT LC have been published, no bibliometric studies conducted summarize prospects and highlight trends hotspots this field. This analysis was investigate provide a systematic understanding development for over past 13 years with aim providing reference new directions strategies LC. Methods: In May 2023, relevant publications from Web Science Core Collection were downloaded, CiteSpace VOSviewer used conduct scientometrics study. Results: total 2,932 articles reviews met inclusion criteria retrieved, China dominated volume 2010 2022 most prolific institution Shanghai Jiao Tong University. Conclusions: can be utilized diagnosis treat Bronchoscopic fluorescence using photosensitizers helps detect tumor changes large bronchial mucosa. future, we should actively explore use photosensitizers, including nanomaterials, addition, combined other treatments significantly prolong survival. combination may also direction future research.

Language: Английский

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Inheritance and innovation of the diagnosis of peripheral pulmonary lesions

Therapeutic Advances in Chronic Disease, Journal Year: 2023, Volume and Issue: 14

Published: Jan. 1, 2023

As the leading cause of cancer-related deaths worldwide, early detection and diagnosis are crucial to reduce mortality lung cancer. To date, peripheral pulmonary lesions (PPLs) remains a major unmet clinical need. The urgency diagnosing PPLs has driven series development advanced bronchoscopy-guided techniques in past decades, such as radial probe-endobronchial ultrasonography (RP-EBUS), virtual bronchoscopy navigation (VBN), electromagnetic (ENB), bronchoscopic transparenchymal nodule access (BTPNA), robotic-assisted bronchoscopy. However, these also have their own limitations. In this review, we would like introduce diagnostic for PPLs, with special focus on biopsy approaches guided by discussing advantages, limitations, future prospects.

Language: Английский

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