Characterisation of the British honey bee metagenome DOI Creative Commons
Tim Regan, Mark Barnett, Dominik R. Laetsch

et al.

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Nov. 20, 2018

The European honey bee (Apis mellifera) plays a major role in pollination and food production. Honey health is complex product of the environment, host genetics associated microbes (commensal, opportunistic pathogenic). Improved understanding these factors will help manage modern challenges to health. Here we used DNA sequencing characterise genomes metagenomes 19 colonies from across Britain. Low heterozygosity was observed many Scottish which had high similarity native dark bee. Colonies exhibited diversity composition relative abundance individual microbiome taxa. Most non-bee sequences were derived known commensal bacteria or pathogens. However, also detected additional fungal, protozoan metazoan species. To classify cobionts lacking genomic information, developed novel network analysis approach for clustering orphan contigs. Our analyses shed light on microbial communities with bees demonstrate power high-throughput, directed metagenomics identifying biological threats agroecosystems.

Language: Английский

Thiacloprid exposure perturbs the gut microbiota and reduces the survival status in honeybees DOI
Yong‐Jun Liu,

Nenghu Qiao,

Qingyun Diao

et al.

Journal of Hazardous Materials, Journal Year: 2019, Volume and Issue: 389, P. 121818 - 121818

Published: Dec. 3, 2019

Language: Английский

Citations

91
Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review DOI Creative Commons
Adriana Nowak,

Daria Szczuka,

Anna Górczyńska

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(3), P. 701 - 701

Published: March 22, 2021

Numerous honeybee (Apis mellifera) products, such as honey, propolis, and bee venom, are used in traditional medicine to prevent illness promote healing. Therefore, this insect has a huge impact on humans’ way of life the environment. While population A. mellifera is large, there concern that widespread commercialization beekeeping, combined with environmental pollution action pathogens, caused significant problems for health populations. One strategies preserve welfare honeybees better understand protect their natural microbiota. This paper provides unique overview latest research features functioning mellifera. Honeybee microbiome analysis focuses both function numerous factors affecting it. In addition, we present characteristics lactic acid bacteria (LAB) an important part gut community special beneficial activities health. The idea probiotics promising tool improve widely discussed. Knowledge microbiota opportunity create broad strategy vitality, including development modern probiotic preparations use instead conventional antibiotics, environmentally friendly biocides, biological control agents.

Language: Английский

Citations

89
The gut microbiota — brain axis of insects DOI
Joanito Liberti, Philipp Engel

Current Opinion in Insect Science, Journal Year: 2020, Volume and Issue: 39, P. 6 - 13

Published: Jan. 28, 2020

Language: Английский

Citations

77
The gut microbiota of bumblebees DOI
Tobin J. Hammer, Eli Le,

A.N. Martin

et al.

Insectes Sociaux, Journal Year: 2021, Volume and Issue: 68(4), P. 287 - 301

Published: Sept. 29, 2021

Language: Английский

Citations

68
Compartmentalization of bacterial and fungal microbiomes in the gut of adult honeybees DOI Creative Commons
Matteo Callegari, Elena Crotti, Marco Fusi

et al.

npj Biofilms and Microbiomes, Journal Year: 2021, Volume and Issue: 7(1)

Published: May 7, 2021

Abstract The core gut microbiome of adult honeybee comprises a set recurring bacterial phylotypes, accompanied by lineage-specific, variable, and less abundant environmental phylotypes. Several mutual interactions functional services to the host, including support provided for growth, hormonal signaling, behavior, are attributed lineage-specific taxa. By contrast, diversity distribution minor phylotypes fungal members in remain overlooked. In present study, we hypothesized that microbial components forager honeybees (i.e., bacteria, members) compartmentalized along portions. such three were investigated context physico-chemical conditions different compartments. We observed changes abundance consistently compartment-specific all components, indicating ecological physiological among host vary with changing metabolic gut.

Language: Английский

Citations

60
The Gut Microbiota Can Provide Viral Tolerance in the Honey Bee DOI Creative Commons

Christopher Dosch,

Anja Manigk,

Tabea Streicher

et al.

Microorganisms, Journal Year: 2021, Volume and Issue: 9(4), P. 871 - 871

Published: April 17, 2021

Adult honey bees host a remarkably consistent gut microbial community that is thought to benefit health and provide protection against parasites pathogens. Currently, however, we lack experimental evidence for the causal role of microbiota in protecting Western (Apis mellifera) their viral Here set out fill this knowledge gap by investigating how modulates virulence major bee pathogen, deformed wing virus (DWV). We found that, upon oral exposure, survival was significantly increased with an experimentally established normal compared control perturbed (dysbiotic) microbiota. Interestingly, titers were similar dysbiotic bees, pointing higher tolerance Taken together, our results positive fitness infection. hypothesize environmental stressors altering composition, e.g., antibiotics beekeeping or pesticides modern agriculture, could interact synergistically pathogens, leading negative effects on epidemiology impact viruses.

Language: Английский

Citations

58
Pesticide-induced disturbances of bee gut microbiotas DOI Open Access
Michelle Hotchkiss, Alexandre J. Poulain, Jessica R. K. Forrest

et al.

FEMS Microbiology Reviews, Journal Year: 2021, Volume and Issue: 46(2)

Published: Nov. 18, 2021

ABSTRACT Social bee gut microbiotas play key roles in host health and performance. Worryingly, a growing body of literature shows that pesticide exposure can disturb these microbiotas. Most studies examine changes taxonomic composition Western honey (Apis mellifera) caused by insecticide exposure. Core microbiota taxa shift abundance after but are rarely eliminated, with declines Bifidobacteriales Lactobacillus near melliventris being the most common shifts. Pesticide concentration, duration, season concurrent stressors all influence whether how disturbed. Also, mechanism disturbance—i.e. directly affects microbial growth or indirectly altering health—likely disturbance consistency. Despite interest this topic, important questions remain unanswered. Specifically, metabolic shifts largely uninvestigated, as do effects pesticide-disturbed on Furthermore, few species have been studied other than A. mellifera, herbicides fungicides examined. We call for knowledge gaps to be addressed so we may obtain comprehensive picture pesticides alter microbiotas, functional consequences changes.

Language: Английский

Citations

56
The gut microbiota affects the social network of honeybees DOI
Joanito Liberti, Tomas Kay, Andrew Quinn

et al.

Nature Ecology & Evolution, Journal Year: 2022, Volume and Issue: 6(10), P. 1471 - 1479

Published: Aug. 22, 2022

Language: Английский

Citations

56
Distinct Roles of Honeybee Gut Bacteria on Host Metabolism and Neurological Processes DOI
Zijing Zhang, Xiaohuan Mu, Shi Yao

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(2)

Published: March 10, 2022

The honeybee possesses a limited number of bacterial phylotypes that play essential roles in host metabolism, hormonal signaling, and feeding behavior. However, the contribution individual gut members shaping brain profiles remains unclear. By generating gnotobiotic bees which were mono-colonized by single bacterium, we revealed different species regulated specific modules metabolites hemolymph. Circulating involved carbohydrate glycerophospholipid metabolism pathways mostly Gilliamella, while Lactobacillus Firm4 Firm5 mainly altered amino acid pathways. We then analyzed transcriptomes with these three bacteria. These showed distinctive gene expression profiles, genes related to olfactory functions labor division upregulated Lactobacillus. Interestingly, differentially spliced brains largely overlapped those unresponsive social stimuli. enriched neural development synaptic transmission. bacteria neurotransmitter levels brain. In particular, dopamine serotonin, show inhibitory effects on sensory sensitivity bees, downregulated bacteria-colonized bees. proboscis extension response normal microbiota is for taste-related behavior honeybees, suggesting potential interactions among host's physiology. Our findings provide fundamental insights into diverse likely contribute neurological processes. IMPORTANCE simple host-restricted community contributes metabolic health its host, symbionts remain elusive. found colonization bee regulates distinct circulating carbohydrate, acid, colonized display transcriptomic crucial behaviors labor. Alternative splicing disordered also mediated. presence promotes sucrose major neurotransmitters being demonstrate how affect behaviors, highlighting gut-brain connections important neurobiological physiological states.

Language: Английский

Citations

50
Mild chronic exposure to pesticides alters physiological markers of honey bee health without perturbing the core gut microbiota DOI Creative Commons
Hanine Almasri, Joanito Liberti, Jean‐Luc Brunet

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: March 11, 2022

Abstract Recent studies highlighted that exposure to glyphosate can affect specific members of the core gut microbiota honey bee workers. However, in this study, bees were exposed relatively high concentrations. Here, we chronically newly emerged imidacloprid, and difenoconazole, individually a ternary mixture, at an environmental concentration 0.1 µg/L. We studied effects these exposures on establishment microbiota, physiological status, longevity, food consumption host. The bacterial species not affected by three pesticides. Negative observed but they restricted few transient non-core species. absence pesticides induced disruption directly altering detoxification system, antioxidant defenses, metabolism Our study indicates even mild alter homeostasis particularly if individuals exhibit dysbiosis (i.e. mostly lack microbiota). This highlights importance early healthy community strengthen natural defenses against xenobiotic stressors.

Language: Английский

Citations

46