Platelet extracellular vesicles-loaded hydrogel bandages for personalized wound care

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Metabolic Reprogramming of CD4+ T Cells by Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuates Autoimmune Hepatitis Through Mitochondrial Protein Transfer

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 9799 - 9819

Published: Sept. 1, 2024

Autoimmune hepatitis (AIH) is a serious liver disease characterized by immune disorders, particularly effector T-cell overactivation. This study aimed to explore the therapeutic effect and underlying mechanism of mesenchymal stem cell-derived extracellular vesicle (MSC-EV) treatment on CD4

Language: Английский

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Unveiling the molecular mechanisms of Danggui-Shaoyao-San against Alzheimer’s disease in APP/PS1 mice via integrating proteomic and metabolomic approaches

Alzheimer s Research & Therapy, Journal Year: 2024, Volume and Issue: 16(1)

Published: Nov. 19, 2024

Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder for which no effective therapy currently available. Given that various attempts to target beta-amyloid (Aβ) have been unsuccessful in clinical trials, other potential pathogenic factors such as brain energy metabolism (EM) attracted increasing attention. Traditional Chinese medicines, including danggui-shaoyao-san (DSS), play a notable role AD. However, it remains unclear whether DSS exerts therapeutic effects on AD through EM regulation. In this study, we conducted behavioural tests, Nissl staining, haematoxylin and eosin thioflavin S APP/PS1 mice assess pharmacodynamic effect of Subsequently, integrated drug network herbal ingredients evaluated their absorption, distribution, metabolism, excretion, toxicity properties identify core ingredients. We used proteomic metabolomic approaches explore mechanisms action against Consequently, verified mechanism underlying using qPCR, western blotting, ELISA. vivo experimental results revealed ameliorated cognitive impairment mice, attenuated neuronal apoptosis, reduced Aβ burden. Furthermore, drug-target comprised 6,514 interactions involving 1,118 218 genes, 253 were identified DSS. The implied could act alleviate AD, targeted metabolomics suggested regulated 47 metabolites associated with EM. Mechanistically, found regulate GSK3β/PGC1α signalling pathway improve glucose uptake mitigate mitochondrial dysfunction oxidative stress, ultimately promoting treat Our study first integrate multi-omics reveal exert promotion EM, thereby providing new insights into

Language: Английский

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Umbilical Cord Mesenchymal Stem Cell-Derived Apoptotic Extracellular Vesicles Improve 5-FU-Induced Delayed Wound Healing by Mitochondrial Transfer

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(4), P. 453 - 453

Published: April 1, 2025

Background/Objectives: This study aimed to explore the therapeutic potential of umbilical mesenchymal stem cell-derived apoptotic vesicles (UMSC-apoVs) in a 5-Fluorouracil (5-FU)-induced impairment skin wound healing. Methods: UMSC-apoVs were isolated from UMSCs using differential centrifugation after induction apoptosis. A murine model was established by administering 5-FU via intravenous tail injection, followed full-thickness creation. Mice received local injections at lesion site. Wound healing evaluated based on closure rates, histological analysis, and vivo/in vitro functional assays. Rotenone (Rot)-pretreated used role mitochondrial transfer between cells (SMSCs) Results: significantly accelerated 5-FU-treated mice, as demonstrated enhanced rates findings reduced inflammatory infiltration increased collagen deposition. transferred mitochondria SMSCs, enhancing viability, proliferation, migration while reducing reactive oxygen species (ROS) production SMSCs. Rot pretreatment inhibited effects inducing dysfunction UMSC-apoVs. Conclusions: Our indicate that improve 5-FU-induced impaired facilitating transfer, suggesting novel strategy for alleviating chemotherapy-induced

Language: Английский

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Platelet Extracellular Vesicles as Natural Delivery Vehicles for Mitochondrial Dysfunction Therapy?

ACS Biomaterials Science & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: April 25, 2025

Mitochondria are vital for energy production, metabolic regulation, and cellular signaling. Their dysfunction is strongly implicated in neurological, cardiovascular, muscular degenerative diseases, where deficits oxidative stress accelerate disease progression. Platelet extracellular vesicles (PEVs), once called "platelet dust", have emerged as promising agents mitigating mitochondrial dysfunction. Like other (EVs), PEVs carry diverse molecular cargo surface markers processes therapeutic efficacy. Notably, they may possibly contain intact or partially functional components, making them tentatively attractive targeting damage. Although direct research on PEVs-mediated rescue remains limited, current evidence suggests that can modulate diseases associated with potentially enhance health. This review explores the potential of neurodegenerative cardiovascular disorders, highlighting their role restoring By examining recent advancements research, we aim to shed light novel strategies utilizing agents. Our goal underscore importance further fundamental applied into PEVs-based interventions, innovative tools combating a wide range linked

Language: Английский

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The role of mitochondrial transfer in the suppression of CD8+ T cell responses by Mesenchymal stem cells

Stem Cell Research & Therapy, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 4, 2024

. CD8+ Cytotoxic T lymphocytes play a key role in the pathogenesis of autoimmune diseases and clinical conditions such as graft versus host disease rejection. Mesenchymal Stromal Cells (MSCs) are multipotent cells with tissue repair immunomodulatory capabilities. Since they able to suppress multiple pathogenic immune responses, MSCs have been proposed cellular therapy for treatment immune-mediated diseases. However, mechanisms underlying their immunosuppressive properties not yet fully understood. remarkable ability sense injury inflammation respond by donating own mitochondria neighboring cells. Whether mitochondrial transfer has any repression responses is unknown. We utilized from Clone 4 TCR transgenic mice that differentiate into effector upon activation vitro vivo address this question. Allogeneic bone marrow derived MSCs, co-cultured activated cells, decreased expansion, production cytokine IFNγ diabetogenic potential vivo. Notably, we found during interaction leading suppression, transferred evidenced FACS confocal microscopy. Transfer MSC also resulted expansion activation. These effects overlapped were additive those prostaglandin E2 secreted MSCs. Furthermore, preventing co-cultures diminished inhibit production. Finally, demonstrated both downregulated T-bet Eomes expression, transcription factors CTL differentiation, on In report showed interact them mitochondria. Mitochondrial contributed global suppressive effect cell downregulating expression resulting impaired

Language: Английский

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Mitochondria transfer for myelin repair

Journal of Cerebral Blood Flow & Metabolism, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Demyelination is a common feature of neuroinflammatory and degenerative diseases the central nervous system (CNS), such as multiple sclerosis (MS). It often linked to disruptions in intercellular communication, bioenergetics metabolic balance accompanied by mitochondrial dysfunction cells oligodendrocytes, neurons, astrocytes, microglia. Although current MS treatments focus on immunomodulation, they fail stop or reverse demyelination’s progression. Recent advancements highlight exchange promising therapeutic target, with potential restore homeostasis, enhance promote myelin repair. With this review we will provide insights into CNS decoupling, focusing role demyelinating conditions. We then discuss emerging cell-free biotherapies exploring transferring mitochondria via biogenic carriers like extracellular vesicles (EVs) synthetic liposomes, aimed at enhancing function support for Lastly, address key challenges clinical application these strategies future directions optimize biotherapies. The field hold promise restoring repair, potentially transforming landscape diseases.

Language: Английский

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Potential Role of Menstrual Fluid‐Derived Small Extracellular Vesicle Proteins in Endometriosis Pathogenesiss

Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(3)

Published: March 1, 2025

ABSTRACT Endometriosis, a chronic debilitating disease affects 1 in 7–10 girls and women, who have symptoms of severe pain subfertility significantly impacts the overall quality life. Currently, no effective early diagnostic methods are available for stages endometriosis. We used menstrual fluid‐derived small extracellular vesicles (MF‐sEVs) from women with self‐reported endometriosis (laparoscopically diagnosed, n = 8) without painful periods ( 9). MF‐sEVs were separated using differential ultracentrifugation characterised nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Western Blot, flow cytometry, mass‐proteomics functional assays. Spherical‐shaped sEVs identified median diameter ∼120 nm, expressing sEV marker proteins. The MF‐sEV proteins classified as endometrial origin. Over 5000 identified, ∼77% which decreased whilst only 22 (largely comprising immunoglobulins) increased endometriosis/MF‐sEVs compared to control/MF‐sEVs. Decreased involved nitrogen compound metabolism, immune response, intracellular signal transduction, regulation programmed cell death, maintenance polarity actin cytoskeleton organisation. Flow cytometry demonstrated significant increase CD86 expression (immune activation marker) endometriosis/MF‐sEVs. Mesothelial cells showed decrease cellular resistance junctional protein expression. possible contributors pathogenesis may potential detection disease.

Language: Английский

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Integrating Mitochondrial Biology into Innovative Cell Therapies for Neurodegenerative Diseases

Brain Sciences, Journal Year: 2024, Volume and Issue: 14(9), P. 899 - 899

Published: Sept. 5, 2024

The role of mitochondria in neurodegenerative diseases is crucial, and recent developments have highlighted its significance cell therapy. Mitochondrial dysfunction has been implicated various disorders, including Alzheimer's, Parkinson's, amyotrophic lateral sclerosis, Huntington's diseases. Understanding the impact mitochondrial biology on these conditions can provide valuable insights for developing targeted therapies. This mini-review refocuses emphasizes potential therapies leveraging mesenchymal stem cells, embryonic induced pluripotent cell-derived secretions, extracellular vesicles. Mesenchymal cell-mediated transfer restoring health cells with dysfunctional mitochondria. Additionally, attention paid to gene-editing techniques such as mito-CRISPR, mitoTALENs, mito-ZNFs, DdCBEs ensure safety efficacy treatments. Challenges future directions are also discussed, possible tumorigenic effects off-target effects, disease targeting, immune rejection, ethical issues.

Language: Английский

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Inter-organ communication is a critical machinery to regulate metabolism and aging

Trends in Endocrinology and Metabolism, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Inter-organ communication (IOC) is a complex mechanism involved in maintaining metabolic homeostasis and healthy aging. Dysregulation of distinct forms IOC linked to derangements age-related pathologies, implicating these processes as potential target for therapeutic intervention promote In this review, we delve into mediated by hormonal signaling, circulating factors, organelle neuronal networks examine their roles regulating metabolism Given the role hypothalamus high-order control center aging longevity, particularly emphasize importance its with peripheral organs pave way better understanding critical machinery

Language: Английский

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